Advanced in vitro exposure systems.

3. Sep. 2013

Characterisation of a Vitrocell® VC 10 in vitro smoke exposure system using dose tools and biological analysis

Chemistry Central Journal 2013, 7:146

doi:10.1186/1752-153X-7-146

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Jason Adamson1, Ken Scott1, Annette Dalrymple1, Clive Meredith1 and Deborah Dillon1

1 British American Tobacco, Group R&D, Southampton, United Kingdom
2 Covance Laboratories Ltd, Harrogate, North Yorkshire, United Kingdom

 

The study demonstrated that the VC 10® can produce stable tobacco smoke dilutions by particulate deposition, measured vapour phase, smoke marker delivery and biological responses from two independent in vitro test systems. 

Read more

Comments (0) Number of views (1428)

29. Jul. 2013

Evaluation method for the cytotoxicity of cigarette smoke byin vitro whole smoke exposure

https://doi.org/10.1016/j.etp.2013.07.004

Xiang Li, Cong Nie, Pingping Shang, Fuwei Xie, Huimin Liu, Jianping Xie

Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China

With the development of direct exposure technology at the air/liquid interface, the toxicological responses of fresh WS can be evaluated. The whole smoke of the 3R4F reference cigarette and two experimental cigarettes were tested on Chinese hamster ovary (CHO) cells to evaluate the endpoints Ames Salmonella reversemutagenicity assay and in vitro micronucleus assay for genotoxicity and neutral red uptake (NRU)assay for cytotoxicity.

 

Read more

Comments (0) Number of views (446)

12. Mar. 2013

Assessment of cigarette smoke particle deposition within the Vitrocell® exposure module using quartz crystal microbalances

Chemistry Central Journal 2013, 7:50

doi:10.1186/1752-153X-7-50

Authors
Jason Adamson, David Thorne, Annette Dalrymple, Debbie Dillon, Clive Meredith

British American Tobacco, Group R&D, Southampton SO15 8TL, UK

 

A successfull technology to quantify the whole smoke particle deposition in vitro, in real-time, is given by the installation of Quartz crystal microbalances in a Vitrocell® Module.

Read more

Comments (0) Number of views (1374)

15. Mar. 2012

Dosimetry Methods for Tobacco Smoke Aersosol In Vitro Exposures

SOT San Francisco, USA

March 11-15, 2012. Poster #1841

Authors
Rainy Marsh1, Jessica Perkins2, Ken Scott2, Debbie Dillon2 and Clive Meredith2

1 British American Tobacco, Group R & D, Southampton, United Kingdom
2 Covance Laboratories Ltd., Harrogate, United Kingdom

 

Successfull experiments in dosimetry of particulate concentration entering the exposure modules to test tobacco smoke on the AMES assay.

Read more

Comments (0) Number of views (1549)

15. Mar. 2012

Impact of test conditions on in vitro cytotoxicity measurements of mainstream cigarette smoke using a whole smoke exposure system

SOT San Francisco, USA

March 11-15, 2012; Poster #1843

Authors
Xiang Li, Pingping Shang, Cong Nie, Fuwei Xie, Huimin Liu, Jianping Xie


Key Laboratory of Tobacco Chemistry, 

Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China

 

This article shows the influence of whole smoke on A549 cells under ISO and HC regimen. The results are shown on the NRU assay. 

Read more

Comments (0) Number of views (1175)

15. Mar. 2012

Utilization of a whole smoke exposure system for the comparison of mainstream cigarette smoke, gas vapor phase and tar mutagenicity

SOT San Francisco, USA

March 11-15, 2012; Poster #1843

Authors
Robert D. Leverette

Life Sciences, A.W. Spears Research Center, Lorillard Tobacco Company, Greensboro, NC 27405, USA

 

This study was conducted to optimize smoke exposure procedures, measure the mutagenicity of mainstream cigarette whole smoke, the gas vapor phase and wet total particulate matter (WTPM) as determined by the Ames Assay.

Read more

Comments (0) Number of views (1438)

16. Dec. 2011

Effects of smoking regimens and test material format on the cytotoxicity of mainstream cigarette smoke

Food Chem Toxicol

2011 Dec 16; 50(3-4):545-551. [Epub ahead of print]

Authors
Li X, Shang P, Peng B, Nie C, Zhao L, Liu H, Xie J.

Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China

 

Total particulate matter or whole smoke generated from different test cigarettes under ISO or HCI regimens were assessed for cytotoxicity using the neutral red uptake cytotoxicity assay. Cytotoxicity among the test cigarettes were greater for whole smoke and the cytotoxicity was decreased going from ISO regimen to HCI regimen. 

Read more

Comments (0) Number of views (1324)

10. Mar. 2011

Comparison of the bacterial mutagenicity of mainstream whole smoke from cigarettes with different levels of menthol

SOT Washington DC, USA

March 06-10, 2011

Authors
Robert D. Leverette

Life Sciences, A. W. Spears Research Center, Lorillard Tobacco Company, Greensboro, NC 27405, USA

 

This study was conducted to compare the mutagenicity of mainstream whole-smoke and wet total particulate matter from two sets of experimental cigarettes to determine if menthol has an effect on this endpoint. All cigarettes were smoked under ISO on a VITROCELL® VC10 smoking robot. TA98 and TA100 were exposed in the VITROCELL® Ames exposure modules.

Read more

Comments (0) Number of views (1379)

10. Mar. 2011

In vitro exposure of organotypical 3D epithelial tissues to cigarette smoke as a potential alternative to rodent inhalation studies

SOT Washington DC, USA

March 06-10, 2011

Authors
Weisensee, D., Kurkowsky, B., Hebestreit, M., Wagner, S. and Schueller J. 

Philip Morris Research Laboratories GmbH, Cologne, Germany, Abstract #998

 

Here we describe an in vitro test system that examines the toxicological effects of single or repeated cigarette smoke exposure on human organo- typically reconstituted 3D epithelial tissues. These tissues were exposed at the air liquid interface to either fresh air or to mainstream smoke from the Reference Cigarette 3R4F in the VITROCELL® system. 

Read more

Comments (0) Number of views (1214)

6. Aug. 2008

News Release

New VITROCELL® cigarette smoking machine for puff by puff analysis

The new development opens up advanced possibilities for research.

Read more

Comments (0) Number of views (799)
RSS
1234567
Back to Top