Advanced in vitro exposure systems.

31. Oct. 2016

Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

doi: 10.1155/2016/9461289
Patrick Geraghty,1,2 Nathalie Baumlin,3 Matthias A. Salathe,3 Robert F. Foronjy,1,2 and Jeanine M. D’Armiento4
1Division of Pulmonary & Critical Care Medicine, Department of Medicine, State University of New York Downstate Medical Center, Brooklyn, NY, USA
2Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY, USA
3Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Miami, Miami, FL, USA
4Center for Pulmonary Disease, Department of Anesthesiology, College of Physicians and Surgeons,Columbia University, New York, NY, USA

 

The Expression of ER stress markers after exposure to cigarette smoke was investigated in fully differentiated normal human bronchial epithelial (NHBE) cells isolated from nonsmoking, smoking, and COPD donors and redifferentiated at the air liquid interface.

 

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9. Oct. 2016

The mutagenic assessment of electronic-cigarettes and tobacco smoke using the Ames assay in strains TA98 and TA100

CORESTA Congress 2016. 9-13 October 2016 - Berlin, Germany

CORESTA Congress, 9-13th October 2016, Germany
Poster No:STPOST34

 

Thorne, D1., Crooks, I1., Hollings, M2., Seymour, A2., Meredith, C1., Gaa, M1.

1 British American Tobacco, R&D Centre, Southampton, SO15 8TL, UK

2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK.

 

The Aim of this study was to assess the mutagenicity of an e-cigarette aerosol, compared to cigarette smoke in tester strains TA98 and TA100 using two different exposure matrices, TPM/eTPM (or ACM – aerosol collected matter) and whole aerosol. A Vitrocell® VC 10 Smoking Robot was used to generate aerosol streams from a traditional reference cigarette (3R4F) and e-cigarette (Vype® ePen). 

 

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9. Oct. 2016

5 out of 7 Tobacco Companies have chosen VITROCELL® for research on combustion and e-cigarettes: A review of aerosol exposure systems relative to the analysis of cytotoxicity

CORESTA Congress 2016. 9-13 October 2016 - Berlin, Germany

CORESTA Congress, 9-13th October 2016, Germany

Poster No:STPOST26

 

David Thorne1, Roman Wieczorek2,Toshiro Fukushima3, Han-Jae Shin4, Robert Leverette5, Mark Ballantyne6, Xiang Li 7, Betsy Bombick5

1 British American Tobacco, Group R&D, Southampton, Hampshire, SO15 8TL, UK;
2 Imperial Brands PLC Company, Reemtsma Cigarettenfabriken GmbH, Albert-Einstein-Ring 7, 22761 Hamburg, Germany;
3 Japan Tobacco Inc, Scientific Product Assessment Centre, 6-2 Umegaoka, Aoba-ku Yokohama, Kanagawa 227-8512, Japan;
4 Korean Tobacco & Ginseng Corporation, 30 Gajeong-ro, Yuseong-gu, Daejeon, 305-805, Republic of Korea;
5 RAI Services Company, 401North Main Street, Winston-Salem, NC 27101, USA;
6 Covance Laboratories Ltd, Otley Road, Harrogate HG3 1PY, UK;
7 Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, No.2 Fengyang Street, High-tech Zone, Zhengzhou, PR China

 

Cytotoxicity data (NRU and MTT) from Kentucky reference 3R4F cigarette smoke were assessed on Balb/C 3T3, CHO-K1, A549, Beas-2B, NCI-H292 and HepG2 cell lines on the Vitrocell Exposure System.

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30. Apr. 2016

Assessing the mutagenic activities of smoke from different cigarettes in direct exposure experiments using the modified Ames Salmonella assay

Shinkichi Ishikawa, Yuki Kanemaru, Hidenori Nara, Kazuo Erami, Yasufumi Nagata

Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2 Umegaoka, Aoba-ku, Yokohama, Kanagawa 227-8512, Japan

 

This article shows the successful exposition exposure of cigarette smoke on two different Ames bacteria strains by using the Vitrocell exposure system including the VC10 smoking robot. Conventional 1 mg tar commercial cigarettes (CC1), 1R5F reference cigarettes (University of Kentucky,Lexington, KY, USA), and 3R4F reference cigarettes (University of Kentucky) were compared with a prototype heated cigarette (HC).

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31. Oct. 2015

Roflumilast partially reverses smoke-induced mucociliary dysfunction

DOI 10.1186/s12931-015-0294-3

Andreas Schmid, Nathalie Baumlin, Pedro Ivonnet, John S. Dennis, Michael Campos, Stefanie Krick and Matthias Salathe*

* Correspondence: msalathe@med.miami.edu
Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Miami Miller School of Medicine, 1600 NW 10th Ave, RMSB #7058, Miami, FL 33136, USA

 

Primary normal human bronchial epithelial cells (NHBE) from non-smokers, cultured at the air-liquid interface (ALI), were exposed to cigarette smoke in a Vitrocell VC-10 smoking robot. The results show that roflumilast can rescue smoke-induced mucociliary dysfunction.Therefor the ELIZA Assay and qPCR were used.

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23. Sep. 2015

A comparative assessment of cigarette smoke aerosols using an in vitro air-liquid interface cytotoxicity test.

DOI:10.3109/08958378.2015.1080773

Authors

Thorne D, Dalrymple A, Dillon D, Duke M, Meredith C

British American Tobacco, Group R&D, Southampton, Hampshire, UK.

 

Evaluation of a modified air-liquid interface BALB/c 3T3 cytotoxicity method for the assessment of smoke aerosols in vitro. 

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15. Jun. 2015

Presentation of new Smoking Machine VITROCELL VC 10® S-TYPE

Cutting edge technologies make the VITROCELL® smoking machine a perfect tool for evaluation of conventional and e-cigarettes.

This smoking machine features all special requirements for high-class research: compliance with ISO, Health Canada and square puff profile protocols as well as ease of handling, fast cleaning and high speed product change capabilities.

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2. Jun. 2015

VITROCELL User Group Meeting 2015

Presentation of new VITROCELL® products and experience exchange among international scientists from Europe, Japan, Korea and USA.

The focus of the event was to present new routes for dosimetry, to give an update on VITROCELL’s participation in major European research programs related to nanoparticles as well as the presentation of new products for 2015.

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22. May. 2015

Testing particle delivery of e-cigarettes

VITROCELL® Application Note for VC 10® S-TYPE Smoking Machine and Particle Photometers

The new application note shows a successfull monitoring of the particle delivery of different e-cigarettes on the setup of a VITROCELL VC 10® S-TYPE Smoking Machine combined with the VITROCELL® Particle Photometer and Monitor Software.

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5. Mar. 2015

The mutagenic assessment of mainstream cigarette smoke using the Ames assay: A multi-strain approach

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

doi:10.1016/j.mrgentox.2015.03.006

Authors
David Thornea, Joanne Kilfordb, Michael Hollingsb, Annette Dalrymplea, Mark Ballantyneb, Clive Mereditha, Deborah Dillona

a British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

 

Wholesmoke Exposure with the VITROCELL® smoking robot VC 10® and the quartz crystal microbalance technology for maintaining the standard Ames Assay

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