Advanced in vitro exposure systems.

11. Mar. 2018

Update on FTIR integration

User Group Meeting 2018

Dr. Martin Kieninger1, Tobias Krebs2

1ANSYCO GmbH, Gasmet Group, 76131 Karlsruhe, Germany

2VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

The poster shows a reliable, easy to use online analysis tool with fast response and a wide range of components due to Calcmet library. Ideal for quality control and dose monitoring.


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11. Mar. 2018

New Products at VITROCELL® User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2018.

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18. Jan. 2018

Extreme testing of undiluted e-cigarette aerosol in vitro using an Ames air-agar-interface technique

https://doi.org/10.1016/j.mrgentox.2018.01.008

D. Thornea, M. Hollingsb, A. Seymourb, J. Adamsona, A. Dalrymplea, M. Ballantyneb, M. Gacaa

a British American Tobacco, R&D, Southampton, Hampshire, SO15 8TL, UK
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

This study investigated the in vitro mutagenicity of e-cigarettes and designed a study to deliver e-cigarette aerosol on an undiluted per puff basis under extreme testing conditions, using the Ames assay across a broader range of bacterial tester strains. The Vitrocell VC 10 smoking robot, previously used to generate and dilute cigarette smoke aerosols was modified to enable the testing of an undiluted e-cigarette aerosol.

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15. Jan. 2018

An approach to testing undiluted e-cigarette aerosol in vitro using 3D reconstituted human airway epithelium

https://doi.org/10.1016/j.tiv.2018.01.010
E. Bishop, L. Haswell, J. Adamson, S. Costigan, D. Thorne, M. Gaca
British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK

A reconstituted 3D human airway epithelium was exposed to undiluted aerosols at the air-liquid interface, using a Vitrocell VC 10 under ISO and HCI conditions. A 3R4F reference cigarette and a commercially available e-cigarette product were tested on their effect on CBF and TEER.

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5. Sep. 2017

Role of Smad3 and p38 Signalling in Cigarette Smoke-induced CFTR and BK dysfunction in Primary Human Bronchial Airway Epithelial Cells

DOI:10.1038/s41598-017-11038-x
Juliette Sailland1, Astrid Grosche1,3, Nathalie Baumlin1, John S. Dennis1, Andreas Schmid1, Stefanie Krick1,2 & Matthias Salathe1
1 Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
2 Division of Pulmonary, Allergy & Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
3 Department of Radiation Oncology, University of Florida, Gainesville, FL, 32610, USA.

The aim of the study is to examine the effects of cigarette smoke on voltage dependent K+ channels, cystic fibrosis transmembrane conductance regulator activity and airway surface liquid volume on primary human bronchial epithelial cells from non-smokers and smokers, cultured at the air-liquid interface to full differentiation.

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27. Jun. 2017

Evaluation of whole cigarette smoke induced oxidative stress in A549 and BEAS–2B cells

DOI:10.1016/j.etap.2017.06.023 

Shimin Zhanga,b, Xiang Lia, Fuwei Xiea, Kejian Liua, Huimin Liua, Jianping Xiea

a Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China
b Technique Center of Tobacco Production, PingDingshanTobacco Company of Henan Tobacco Monopoly Bureau, PingDingshan 467000, China

The purpose of this study is to evaluate oxidative stress induced by whole cigarette smoke based on the air-liquid interface exposure system. The cytotoxicity of whole smoke were measured by MTT and NRU assays, and several intracellular and extracellular oxidative stress biomarkers such as GSH and GSSG, MDA, HNE, EC-SOD and 8-OHdG were investigated in A549 and BEAS–2B cells.

 

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15. Mar. 2017

New Products 2017 presented at VITROCELL® User Group Meeting

10 March 2017, Hilton Baltimore Inner Harbor Hotel, USA

Following the VITROCELL® User Group Meeting 2016 in New Orleans, international scientists from Canada, Europe, Japan, Korea and USA reconvened in March 2017.

The meeting offered an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange experiences in working with VITROCELL® devices and to meet fellow researchers. It was held prior to the International Society of Toxicology 56th Annual Meeting and ToxExpo – one of the largest international conferences related to toxicology.

Focus of the event was to exchange latest research results, to give an update on VITROCELL’s participation in major European and German research programs as well as the presentation of new products for 2017.

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31. Jan. 2017

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

DOI: 10.1089/aivt.2016.0025

Jason Adamson1,* Xiang Li2,* Huapeng Cui2, David Thorne1, Fuwei Xie2, and Marianna Gaca1
1British American Tobacco, R&D, Southampton, United Kingdom.
2Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, PR China.
*Joint first authors.

A VC 10 Smoking Robot was designated to look at the generated aerosol nicotine concentration from different e-cigarettes compared with the 3R4F reference cigarette in two different laboratories. The aerosol was trapped and the nicotine concentration was analysed by different kinds of mass spectometry.
Nicotine assessment across the tested e-cigarette categories showed consistent delivery of nicotine per puff within products and that the method was sensitive enough to detect different levels of nicotine across products. Good overlap in nicotine results were obtained in the two different laboratories.

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3. Jan. 2017

Development, qualification, validation and application of the neutral red uptake assay in Chinese Hamster Ovary (CHO) cells using a VITROCELL® VC10® smoke exposure system

Wanda Fields a, Kathy Fowler a, Victoria Hargreaves b, Lesley Reeve b, Betsy Bombick a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street,Winston-Salem, NC 27101, USA
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG1 3PY, UK

This study discribes the development, validation and application of the NRU assay in Chinese Hamster Ovary (CHO) cells, following exposure to fresh whole smoke generated with the VITROCELL®VC10®system. The VITROCELL® VC10® successfully provided consistent generation and delivery of whole smoke from a cigarette (3R4F) and a primarily “heat-not-burn” cigarette (Eclipse).

 

 

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7. Dec. 2016

The comparative in vitro assessment of e-cigarette and cigarette smoke aerosols using the gH2AX assay and applied dose measurements

http://dx.doi.org/10.1016/j.toxlet.2016.12.006

David Thorne, Sophie Larard, Andrew Baxter, Clive Meredith, Marianna Gaҫa

British American Tobacco, R&D Centre, Southampton, Hampshire, SO15 8TL, UK

 

H I G H L I G H T S
Cigarette smoke generates a dose dependent increase in double strand breaks.
E-cigarette aerosols did not generate any increases in double strand breaks above control levels.
E-cigarette exposures were approximately 28-fold greater than that of cigarette smoke exposures.
In vitro dosimetry ensures accurate interpretation of the data, and confirms delivery of test agent.

In vitro dosimetry allows cross-product, study and system comparisons.

 

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