Advanced in vitro exposure systems.

30. Oct. 2014

Comparative In Vitro Toxicity Profile of Electronic and Tobacco Cigarettes, Smokeless Tobacco and Nicotine Replacement Therapy Products: E-Liquids, Extracts and Collected Aerosols

International Journal of Environmental Research and Public Health 2014

11325-11347; doi:10.3390/ijerph111111325

Authors
Manoj Misra, Robert D. Leverette, Bethany T. Cooper, Melanee B. Bennett and Steven E. Brown

Lorillard Tobacco Company, A.W. Spears Research Center, 420 North English Street, Greensboro, North Carolina 27405, USA

 

The in vitro exposure of tobacco and e-cigarettes that were smoked on a VITROCELL VC 10® smoking robot. The Results are shown with the NRU, IL-8, Ames and Micronucleus assay.

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30. Oct. 2014

An inter-machine comparison of tobacco smoke particle deposition in vitro from six independent smoke exposure systems

Toxicology in Vitro

Volume 28, Issue 7, October 2014, Pages 1320-1328 (doi: 10.1016/j.tiv.2014.06.012)

Authors
J. Adamsona, D. Thornea, G. Erringtona, W. Fieldsb, X. Lic, , R. Payned, T. Krebse, A. Dalrymplea, K. Fowlerb, D. Dillona, F. Xiec, , C. Mereditha

a British American Tobacco, Group R&D, Southampton SO15 8TL, UK
b R.J. Reynolds Tobacco Co., P.O. Box 1487, Winston-Salem, NC 27102, USA
c Zhengzhou Tobacco Research Institute of China National Tobacco Corporation,
No.2 Fengyang Street, High-Tech Zone, Zhengzhou, PR China
d Covance Laboratories Ltd., Otley Road, Harrogate HG3 1PY, UK
e Vitrocell® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany

 

Comparison of different smoke exposure systems to demonstrate VC 10® suitability for tobacco smoke exposure in vitro. The results are shown in the particle deposition across the modules.

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15. Jul. 2014

A method for assessment of the genotoxicity of mainstream cigarette-smoke by use of the bacterial reverse-mutation assay and an aerosol-based exposure system

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Volume 769, 15 July 2014, Pages 20–28, [epub ahead of print]

Authors
Joanne Kilforda, David Thorneb, Rebecca Paynea, Annette Dalrympleb, Julie Clementsa, Clive Meredithb, Debbie Dillonb

a Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK
b British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK

Successfull modifikation of the Ames assay for the testing of a mainstream cigarette smoke aerosol that is generated with a VC 10® exposure system. The reproducebility of the response on a second VC 10® system is shown.

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30. Jun. 2014

Transforming Growth Factor β1 and Cigarette Smoke Inhibit the Ability of β2-Agonists to Enhance Epithelial Permeability

www.atsjournals.org published on June 30, 2014 (doi: 10.1165/rcmb.2013-0538OC)

Authors
Hoshang J. Unwalla1, Pedro Ivonnet1, John S. Dennis, Gregory E. Conner1,2, and Matthias Salathe1
1Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, and
2Department of Cell Biology, University of Miami, Miller School of Medicine, Miami, Florida

Exposition of whole smoke on human bronchial epithelial cells in the air-liquid interface with attention on growth factor TGF-β1.

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17. Jun. 2014

Development of a BALB/c 3T3 neutral red uptake cytotoxicity test using a mainstream cigarette smoke exposure system

BioMed Central

BMC Research Notes 2014, 7:367 (doi:10.1186/1756-0500-7-367)

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Linsey Haswell1, Annette Dalrymple1, Clive Meredith1 and Deborah Dillon1

1 British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

Mainstream cigarette smoke and the gase vapour phase, generated with the VC 10® smoke exposure system, indicates significant differently in-vitro cytotoxicity profiles. The used method on the air-liquid interface takes into account the synergies of both the particulate and vapour phase of tobacco smoke in the NRU assay.

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2. Jun. 2014

Application of a modified gaseous exposure system to the in vitro toxicological assessment of tobacco smoke toxicants

Environmental and Molecular Mutagenesis

Volume 55, Issue 8, Article first published online: 2 JUN 2014 (doi: 10.1002/em.21876)

Authors
Damien Breheny1, Fiona Cunningham1, Joanne Kilford2, Rebecca Payne2, Deborah Dillon1 and Clive Meredith1

1 British American Tobacco, Group R&D, Southampton, Hampshire, United Kingdom
2 Covance Laboratories Ltd, North Yorkshire, United Kingdom

Demonstration of the suitability of the VC 10® exposure system for the assesment of the mutagenic potential of gases using the AMES Assay. Not only cigarette smoke, also a wide range of gaseous compounds and aerosol mixtures can be studied.

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27. Mar. 2014

Potential toxicity of electronic cigarette liquids and aerosols as measured by four in vitro assays

SOT Phoenix, USA

24-27 March, 2014

Authors
Leverette, R.D., Misra, M., Cooper, B.T. and Bennett, M.B.

Lorillard Tobacco Company, A.W. Spears Research Center, Greensboro, NC 27405, USA

Exposition of tobacco and e-cigarette smoke, that were smoked on a VC 10® smoking robot in combination with a Vitrocell laser photometer for caculation of the delivered dose. The cytotoxicity was measured in four different endpoints: AMES, NRU, IL-8 Release and Micronucleus Assay.

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1. Nov. 2013

A review of in vitro cigarette smoke exposure systems

Experimental and Toxicologic Pathology

Volume 65, Issues 7-8, November 2013, Pages 1183-1193. pii: S0940-2993(13)00081-X. doi: 10.1016/j.etp.2013.06.001. [Epub ahead of print]

Authors
Thorne D, Adamson J.

British American Tobacco, Group R&D, Southampton SO15 8TL, UK.

In this review, the authors present a summary of the major tobacco smoke exposure systems available and critically review their function, set-up and application for in vitro exposure scenarios.

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3. Oct. 2013

A modified Ames methodology for the assessment of mainstream cigarette smoke genotoxicity using an aerosol-based exposure system

Poster CORESTA SSPT Meeting. Seville, Spain.

29 September - 3 October 2013

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Annette Dalrymple1, Julie Clements2, Clive Meredith1, Debbie Dillon1

1 British American Tobacco, Group R&D, Southampton, United Kingdom
2 Covance Laboratories Ltd, Harrogate, North Yorkshire, United Kingdom

 

The successfull exposure of whole smoke on a modified AMES assay using the the air-liquid interface exposure system. 

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6. Sep. 2013

Comet assay and air-liquid interface exposure system: A new combination to evaluate genotoxic effects of cigarette whole smoke in human lung cell lines

Toxicology in Vitro

Volume 27, Issue 6, September 2013, Pages 1987-1991. pii: S0887-2333(13)00168-9. doi: 10.1016/j.tiv.2013.06.016. [Epub ahead of print]

Authors
Weber S.1, Hebestreit M.1,3, Conroy L.L.1, Rodrigo G.2

1 Philip Morris International R&D, Philip Morris Research Laboratories GmbH, Fuggerstr.3, 51149 Cologne, Germany
2 Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
3 Current address: VITROCELL Systems GmbH, Germany

 

Human lung cell lines A549 and BEAS-2B were exposed to diluted whole smoke in the VITROCELL® 24 and DNA damage was evaluated using the comet assay.

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