Advanced in vitro exposure systems.

27. Jul. 2020

Vitrocell® holder systems for e-cigarettes

Secure and tight connection of any device to the smoking machine.

New designs of electronic cigarettes such as ENDS (Electronic Nicotine Delivery Systems) products or HTP (Heated Tobacco Products) lead to a large variety of different shapes which make the insertion into conventional holders with labyrinth seals impossible. VITROCELL® has developed a new holder system which is flexible to adjust to different shapes.

VITROCELL Application Note

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13. Jul. 2020

An experimental aerosol air–agar interface mouse lymphoma assay methodology

DOI: https://doi.org/10.1016/j.mrgentox.2020.503230 

Authors
Thorne D1, Hollings M2, Kilford J2, Clements J2, Payne R2, Ballantyne M2, Dalrymple, A1, Dillon, D1, Meredith, C1, Gaҫa, M1  
 
1 British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK. 
2 Covance Laboratories Ltd, Otely Road, Harrogate, HG1 PY, UK

 

Highlights

  • An experimental concept of exposing L5178Y cells at the air-agar-interface
  • Cells were lightly seeded on an agar surface to achieve exposure
  • Flowing air, plating and recovery efficiencies from the agar-surface were investigated
  • Positive controls were responsive, suggesting a clear cellular interaction with the agar-matrix 
  • Cigarette smoke aerosol showed a dose-response relationship with increasing mutant frequencies

 

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24. Feb. 2020

In vitro advances in whole aerosol approaches for e-cigarette testing

IIVS Workshop 3, Feb 24-26th 2020

Autor

David Thorne, British American Tobacco

 

Workshop series to identify, discuss and develop recommendations for the optimal generation and use of in vitro data for product regulation.

 

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4. Nov. 2019

Investigation of multiple whole smoke dosimetry techniques using a VITROCELL®VC10® smoke exposure system

https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keysera, Robert Leverettea, Michael Hollingsb, Adam Seymourb, Lesley Reeveb, Wanda Fieldsa
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
b Covance Laboratories Ltd., North Yorkshire, UK

 

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

 

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25. Oct. 2019

VITROCELL® High Throughput Exposure Module: Deposition and Cytotoxicity of Smoke/Aerosol from Different Tobacco Product Types

User Group Meeting 2019

1Leverette R, 1Keyser B, 2Seymour A, 2Hollings M and 1Bombick B
1Scientific & Regulatory Affairs, RAI Services Company Winston-Salem, NC 27102
2Covance Laboratories Ltd Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

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25. Oct. 2019

New holder for ENDS products for VITROCELL® Smoking Machines and Robots

User Group Meeting 2019

Bastian Gutmann, Tobias Krebs
VITROCELL Systems GmbH 79183 Waldkirch, Germany

The poster shows the new holder for ENDS products

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10. Mar. 2019

New Products at VITROCELL® User Group Meeting 2019

09 March 2019, Hilton Inner Harbour Hotel, Baltimore, USA

Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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18. Dec. 2018

Development of a quantitative method for assessment of dose in in vitro evaluations using a VITROCELL® VC10® smoke exposure system

https://doi.org/10.1016/j.tiv.2018.12.010

Brian M. Keysera, Robert Leverettea, Kathy Fowlera, Wanda Fieldsa, Victoria Hargreavesb, Lesley Reeveb, Betsy Bombicka
a RAI Services Company1, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
b Covance Laboratories Ltd., North Yorkshire, UK

The aims of this study were to investigate dosimetry during whole smoke exposure in the VITROCELL® VC10® smoking robot using QCMs and to determine whether photometers could be used for concurrent assessment of dose during in vitro whole smoke exposures.

 

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28. Oct. 2018

Big tobacco embraces in vitro toxicology

Manufacturers invest in nonanimal tests to evaluate health risks of e-cigarettes and other next-generation tobacco products

c&en, Volume 96, Issue 43

Britt E. Erickson

The complexity of next-generation tobacco products has increased by a number of factors since FDA was given responsibility to regulate tobacco. Testing e-liquids with animals is not practical, economically and not that accurate. The Vitrocell system generates airborne materials from test products, such as smoke from reference cigarettes and vapor from e-cigarettes, in a manner that simulates how a person would puff. This artcile shows, how IIVS scientists use human donor tissues, obtained from organs, to create cell cultures and in vitro test systems.


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3. May. 2018

Application of a direct aerosol exposure system for the assessment of biological effects of cigarette smoke and novel tobacco product vapor on human bronchial epithelial cultures

https://doi.org/10.1016/j.yrtph.2018.05.004

Shinkichi Ishikawa∗, Kazushi Matsumura, Nobumasa Kitamura, Kanae Ishimori, Yuichiro Takanami, Shigeaki Ito

Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2 Umegaoka, Aoba-ku, Yokohama, Kanagawa 227-8512, Japan

The experimental setup for the aerosol exposure consisted of a VC 10 smoking robot, a dilution system, and a Vitrocell 12/3 glass module the exposure of nicotine and TPM in the mainstream aerosols from 3R4F and novel tobacco vapor on MucilAir™ cells. The results are shown in cytotoxicity, IL-8, IL-1ß and gene expression.


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