¹Leverette R, ¹Keyser B, ²Seymour A, ²Hollings M and ¹Bombick B
¹Scientific & Regulatory Affairs, RAI Services Company Winston-Salem, NC 27102
²Covance Laboratories Ltd Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

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Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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c&en, Volume 96, Issue 43

The complexity of next-generation tobacco products has increased by a number of factors since FDA was given responsibility to regulate tobacco. Testing e-liquids with animals is not practical, economically and not that accurate. The Vitrocell system generates airborne materials from test products, such as smoke from reference cigarettes and vapor from e-cigarettes, in a manner that simulates how a person would puff. This artcile shows, how IIVS scientists use human donor tissues, obtained from organs, to create cell cultures and in vitro test systems.

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https://doi.org/10.1016/j.toxrep.2018.04.003

Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a,
^a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA2
^b Covance Laboratories Ltd., North Yorkshire, UK

The article shows the successfull qualification of the Vitrocell VC10 smoking robot. The robot was used to  expose bacterial cells to cigarette whole smoke generated from 3R4F reference cigarettes and Eclipse cigarettes. A comparison was made between whole smoke from a tobacco heating and a combustible cigarette.

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DOI: 10.1089/aivt.2018.0001
Holger Behrsing,¹ Mario Aragon,¹ Jason Adamson,² Devin Sheehan,¹ Marianna Gaca,² Rodger Curren,¹ and Erin Hill^1
1Respiratory Toxicology, Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.
²R&D, British American Tobacco, Southampton, United Kingdom.

The study shows an assessment of VC1 aerosol generation that was conducted by measuring puff-by-puff in vitro nicotine concentration of 3R4F cigarettes (ISO and HCI regimes), e-cigarette (CRM81 puffing profile), and THP (HCIm regime). The aerosols were applied to in vitro or ex vivo cellular and tissue systems that model the respiratory tract, such as reconstructed human airways (RHuA), which are grown at the air–liquid interface (ALI). Additionally a comparison between the Vitrocell VC1 (U.S. Laboratory) and the VC10 (UK Laboratory) Smoking Robot was done.

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