DOI: https://doi.org/10.1016/j.mrgentox.2020.503230 

Authors
Thorne D¹, Hollings M², Kilford J², Clements J², Payne R², Ballantyne M², Dalrymple, A¹, Dillon, D¹, Meredith, C¹, Gaҫa, M^{1  
 
1} British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK. 
² Covance Laboratories Ltd, Otely Road, Harrogate, HG1 PY, UK

Highlights

• An experimental concept of exposing L5178Y cells at the air-agar-interface
• Cells were lightly seeded on an agar surface to achieve exposure
• Flowing air, plating and recovery efficiencies from the agar-surface were investigated
• Positive controls were responsive, suggesting a clear cellular interaction with the agar-matrix 
• Cigarette smoke aerosol showed a dose-response relationship with increasing mutant frequencies

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https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keyser^a, Robert Leverette^a, Michael Hollings^b, Adam Seymour^b, Lesley Reeve^b, Wanda Fields^a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
^b Covance Laboratories Ltd., North Yorkshire, UK

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

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Bastian Gutmann, Tobias Krebs
VITROCELL Systems GmbH 79183 Waldkirch, Germany

The poster shows the new holder for ENDS products

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https://doi.org/10.1016/j.tiv.2018.12.010

Brian M. Keyser^a, Robert Leverette^a, Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a
a RAI Services Company1, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
^b Covance Laboratories Ltd., North Yorkshire, UK

The aims of this study were to investigate dosimetry during whole smoke exposure in the VITROCELL® VC10® smoking robot using QCMs and to determine whether photometers could be used for concurrent assessment of dose during in vitro whole smoke exposures.

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https://doi.org/10.1016/j.yrtph.2018.05.004

Shinkichi Ishikawa∗, Kazushi Matsumura, Nobumasa Kitamura, Kanae Ishimori, Yuichiro Takanami, Shigeaki Ito

The experimental setup for the aerosol exposure consisted of a VC 10 smoking robot, a dilution system, and a Vitrocell 12/3 glass module the exposure of nicotine and TPM in the mainstream aerosols from 3R4F and novel tobacco vapor on MucilAir™ cells. The results are shown in cytotoxicity, IL-8, IL-1ß and gene expression.

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