Advanced in vitro exposure systems.

5. Mar. 2015

The mutagenic assessment of mainstream cigarette smoke using the Ames assay: A multi-strain approach

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

doi:10.1016/j.mrgentox.2015.03.006

Authors
David Thornea, Joanne Kilfordb, Michael Hollingsb, Annette Dalrymplea, Mark Ballantyneb, Clive Mereditha, Deborah Dillona

a British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

 

Wholesmoke Exposure with the VITROCELL® smoking robot VC 10® and the quartz crystal microbalance technology for maintaining the standard Ames Assay

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12. Feb. 2015

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

New video protocol Exposure of 3D cell cultures using VITROCELL® 24/48 system

jove, 12 Februray 2015, Issue 96 (doi: 10.3791/52325)

Authors
Diana Kuehn1, Shoaib Majeed1, Emmanuel Guedj1, Remi Dulize1, Karine Baumer1, Anita Iskandar1, Stephanie Boue1, Florian Martin1, Radina Kostadinova1, Carole Mathis1, Nikolai V. Ivanov1, Stefan Frentzel1, Julia Hoeng1, Manuel C. Peitsch1

1 Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A

The aim of this video protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke at the air-liquid interface to measure ciliary beating and TEER.

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12. Nov. 2014

Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke

Chemistry Central Journal 2014, 8:62

doi:10.1186/s13065-014-0062-3

Authors
Shoaib Majeed1, Stefan Frentzel1, Sandra Wagner2, Diana Kuehn1, Patrice Leroy1, Philippe A. Guy1, Arno Knorr1, Julia Hoeng1 and Manuel C. Peitsch1

1 Philip Morris Research and Development, Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
2 Eurofins Umwelt West GmbH, Vorgebirgsstraße20, D-50389 Wesseling, Germany

 

The connection of a VITROCELL® 24/48 system to a 30-port carousel smoking machine is shown. The exposure is to verify the cigarette smoke aerosol distribution, the utility of VITROCELL® crystal quartz microbalances and the amount of particles deposited per surface area. The results are shown in cell viability and WST-1 oxidation.

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30. Oct. 2014

An inter-machine comparison of tobacco smoke particle deposition in vitro from six independent smoke exposure systems

Toxicology in Vitro

Volume 28, Issue 7, October 2014, Pages 1320-1328 (doi: 10.1016/j.tiv.2014.06.012)

Authors
J. Adamsona, D. Thornea, G. Erringtona, W. Fieldsb, X. Lic, , R. Payned, T. Krebse, A. Dalrymplea, K. Fowlerb, D. Dillona, F. Xiec, , C. Mereditha

a British American Tobacco, Group R&D, Southampton SO15 8TL, UK
b R.J. Reynolds Tobacco Co., P.O. Box 1487, Winston-Salem, NC 27102, USA
c Zhengzhou Tobacco Research Institute of China National Tobacco Corporation,
No.2 Fengyang Street, High-Tech Zone, Zhengzhou, PR China
d Covance Laboratories Ltd., Otley Road, Harrogate HG3 1PY, UK
e Vitrocell® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany

 

Comparison of different smoke exposure systems to demonstrate VC 10® suitability for tobacco smoke exposure in vitro. The results are shown in the particle deposition across the modules.

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8. Oct. 2014

The Response of Human Nasal and Bronchial Organotypic Tissue Cultures to Repeated Whole Cigarette Smoke Exposure

International Journal of Toxicology (October 8, 2014)

Authors
Marja Talikka1, Radina Kostadinova1, Yang Xiang1, Carole Mathis1, Alain Sewer1, Shoaib Majeed1, Diana Kuehn1, Stefan Frentzel1, Celine Merg1, Marcel Geertz1, Florian Martin1, Nikolai V. Ivanov1, Manuel C. Peitsch1, Julia Hoeng1

1 Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

 

Whole Smoke Exposure on nasal and bronchial cells using a VITROCELL®24 system in comparison to animal testings.

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21. Jul. 2014

In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

informa healthcare

informa healthcare, posted online on July 21, 2014. (doi:10.3109/15376516.2014.943441)

Authors
Walter K. Schlage*, Anita R. Iskandar*, Radina Kostadinova*, Yang Xiang*, Alain Sewer*, Shoaib Majeed, Diana Kuehn, Stefan Frentzel, Marja Talikka, Marcel Geertz, Carole Mathis, Nikolai Ivanov, Julia Hoeng, and Manuel C. Peitsch

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

*Equal contribution

Exposition of cigarette smoke to tissue cultures at the air–liquid interface shows in combination of the microarray technology, gene-set analysis and a novel computational modeling an increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity. The usefulness of in vitro exposure to buccal and gingival organotypic epithelial tissue cultures for the impact assessment of cigarette smoke is demonstrated. 

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15. Jul. 2014

A method for assessment of the genotoxicity of mainstream cigarette-smoke by use of the bacterial reverse-mutation assay and an aerosol-based exposure system

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Volume 769, 15 July 2014, Pages 20–28, [epub ahead of print]

Authors
Joanne Kilforda, David Thorneb, Rebecca Paynea, Annette Dalrympleb, Julie Clementsa, Clive Meredithb, Debbie Dillonb

a Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK
b British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK

Successfull modifikation of the Ames assay for the testing of a mainstream cigarette smoke aerosol that is generated with a VC 10® exposure system. The reproducebility of the response on a second VC 10® system is shown.

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30. Jun. 2014

Transforming Growth Factor β1 and Cigarette Smoke Inhibit the Ability of β2-Agonists to Enhance Epithelial Permeability

www.atsjournals.org published on June 30, 2014 (doi: 10.1165/rcmb.2013-0538OC)

Authors
Hoshang J. Unwalla1, Pedro Ivonnet1, John S. Dennis, Gregory E. Conner1,2, and Matthias Salathe1
1Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, and
2Department of Cell Biology, University of Miami, Miller School of Medicine, Miami, Florida

Exposition of whole smoke on human bronchial epithelial cells in the air-liquid interface with attention on growth factor TGF-β1.

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17. Jun. 2014

Development of a BALB/c 3T3 neutral red uptake cytotoxicity test using a mainstream cigarette smoke exposure system

BioMed Central

BMC Research Notes 2014, 7:367 (doi:10.1186/1756-0500-7-367)

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Linsey Haswell1, Annette Dalrymple1, Clive Meredith1 and Deborah Dillon1

1 British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

Mainstream cigarette smoke and the gase vapour phase, generated with the VC 10® smoke exposure system, indicates significant differently in-vitro cytotoxicity profiles. The used method on the air-liquid interface takes into account the synergies of both the particulate and vapour phase of tobacco smoke in the NRU assay.

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2. Jun. 2014

Application of a modified gaseous exposure system to the in vitro toxicological assessment of tobacco smoke toxicants

Environmental and Molecular Mutagenesis

Volume 55, Issue 8, Article first published online: 2 JUN 2014 (doi: 10.1002/em.21876)

Authors
Damien Breheny1, Fiona Cunningham1, Joanne Kilford2, Rebecca Payne2, Deborah Dillon1 and Clive Meredith1

1 British American Tobacco, Group R&D, Southampton, Hampshire, United Kingdom
2 Covance Laboratories Ltd, North Yorkshire, United Kingdom

Demonstration of the suitability of the VC 10® exposure system for the assesment of the mutagenic potential of gases using the AMES Assay. Not only cigarette smoke, also a wide range of gaseous compounds and aerosol mixtures can be studied.

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