https://doi.org/10.1016/j.toxlet.2020.11.006

Albert Giralt^a, Anita R Iskandar^a, FlorianMartin^a, ElisaMoschini^b, TomassoSerchi^b, AthanasiosKondylis^a, DiegoMarescotti^a, PatriceLeroy^a, LauraOrtega-Torres^a, ShoaibMajeed^a, CelineMerg^a, KeyurTrivedi^a, EmmanuelGuedj^a,StefanFrentzel^a, Nikolai V Ivanov^a, Manuel C Peitsch^a, Arno C Gutleb^b, JuliaHoeng^a

^a Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland
^b Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Luxembourg

Highlights
• Biological impact of exposure to an aerosol of an e-vapor device was investigated using human bronchial and alveolar models.
• The biological impact of e-vapor aerosol exposure was lower than that of cigarette smoke at similar nicotine levels.
• The exposure effects at the molecular levels were evaluated using a systems toxicology approach.

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https://doi.org/10.1016/j.envres.2020.110350

Emeline Seurat ^a, Anthony Verdin ^b, Fabrice Cazier ^c, Dominique Courcot ^b, Richard Fitoussi ^d, Katell Vi´e ^d, Val´erie Desauziers ^e, Isabelle Momas ^a, Nathalie Seta ^a, Sophie Achard ^a
a Laboratoire de Sant´e Publique et Environnement, Hera “Health Environmental Risk Assessment”, Inserm UMR1153-CRESS (Centre de Recherche en Epid´emiologie et StatistiqueS), Universit´e de Paris, Facult´e de Pharmacie de Paris, 4, Avenue de L’Observatoire, 75006, Paris, France
^b Unit´e de Chimie Environnementale et Interactions sur le Vivant UR4492, SFR Condorcet FR CNRS 3417, Maison de La Recherche en Environnement Industriel 2, Universit´e Du Littoral Cˆote D’Opale, 189A Avenue Maurice Schumann, 59140, Dunkerque, France
^c Centre Commun de Mesures (CCM), Universit´e Du Littoral-Cˆote D’Opale, 145 Avenue Maurice Schumann, 5914, Dunkerque, France
^d Laboratoires Clarins, 5 Rue Amp`ere, 95300, Pontoise, France
^e IPREM, IMT Mines Ales, Universit´e de Pau et des Pays de L’Adour, E2S UPPA, CNRS, Pau, France

In the course of this study, they tested various pollutants with different chemical compositions, applying them to the apical side of Reconstructed Human Epidermis and being particularly interested in the effect relative humidity has on the reaction to pollutants. Investigating several cytokines and chemokines, they showed that IL-1α, IL-6, IL-8, and RANTES are the cytokines/chemokines almost systematically induced by most pollutants.

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New designs of electronic cigarettes such as ENDS (Electronic Nicotine Delivery Systems) products or HTP (Heated Tobacco Products) lead to a large variety of different shapes which make the insertion into conventional holders with labyrinth seals impossible. VITROCELL® has developed a new holder system which is flexible to adjust to different shapes.

VITROCELL Application Note

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DOI: https://doi.org/10.1016/j.mrgentox.2020.503230 

Authors
Thorne D¹, Hollings M², Kilford J², Clements J², Payne R², Ballantyne M², Dalrymple, A¹, Dillon, D¹, Meredith, C¹, Gaҫa, M^{1  
 
1} British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK. 
² Covance Laboratories Ltd, Otely Road, Harrogate, HG1 PY, UK

Highlights

• An experimental concept of exposing L5178Y cells at the air-agar-interface
• Cells were lightly seeded on an agar surface to achieve exposure
• Flowing air, plating and recovery efficiencies from the agar-surface were investigated
• Positive controls were responsive, suggesting a clear cellular interaction with the agar-matrix 
• Cigarette smoke aerosol showed a dose-response relationship with increasing mutant frequencies

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Robert Leverette¹, Brian Keyser¹, Michael Hollings² and Adam Seymour^2
1RAI Services Company, Winston−Salem, NC 27101 USA
²Covance Laboratories Ltd., Harrogate, North Yorkshire HG3 1PY, UK

• Freshly generated whole smoke / aerosol from three different tobacco product types (3R4F, THP and ENDS) was consistently delivered within the AMES 48 and 6/48 exposure modules.
• Biological endpoints: 3R4F whole smoke induced increased revertant counts (Ames) and cytotoxicity (NRU) in the AMES 48 and 6/48 modules, respectively.
• Revertant counts (Ames) and cytotoxicity (IC50) values were comparable to those from the standard exposure modules when run under similar exposure conditions.
• Overall, the AMES 48 and 6/48 modules are deemed “Fit for Use" for the in vitrO evaluation of different tobacco product types (combustible, THP, ENDS).
 

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