https://doi.org/10.1016/j.envres.2020.110350

Emeline Seurat ^a, Anthony Verdin ^b, Fabrice Cazier ^c, Dominique Courcot ^b, Richard Fitoussi ^d, Katell Vi´e ^d, Val´erie Desauziers ^e, Isabelle Momas ^a, Nathalie Seta ^a, Sophie Achard ^a
a Laboratoire de Sant´e Publique et Environnement, Hera “Health Environmental Risk Assessment”, Inserm UMR1153-CRESS (Centre de Recherche en Epid´emiologie et StatistiqueS), Universit´e de Paris, Facult´e de Pharmacie de Paris, 4, Avenue de L’Observatoire, 75006, Paris, France
^b Unit´e de Chimie Environnementale et Interactions sur le Vivant UR4492, SFR Condorcet FR CNRS 3417, Maison de La Recherche en Environnement Industriel 2, Universit´e Du Littoral Cˆote D’Opale, 189A Avenue Maurice Schumann, 59140, Dunkerque, France
^c Centre Commun de Mesures (CCM), Universit´e Du Littoral-Cˆote D’Opale, 145 Avenue Maurice Schumann, 5914, Dunkerque, France
^d Laboratoires Clarins, 5 Rue Amp`ere, 95300, Pontoise, France
^e IPREM, IMT Mines Ales, Universit´e de Pau et des Pays de L’Adour, E2S UPPA, CNRS, Pau, France

In the course of this study, they tested various pollutants with different chemical compositions, applying them to the apical side of Reconstructed Human Epidermis and being particularly interested in the effect relative humidity has on the reaction to pollutants. Investigating several cytokines and chemokines, they showed that IL-1α, IL-6, IL-8, and RANTES are the cytokines/chemokines almost systematically induced by most pollutants.

Read more

New designs of electronic cigarettes such as ENDS (Electronic Nicotine Delivery Systems) products or HTP (Heated Tobacco Products) lead to a large variety of different shapes which make the insertion into conventional holders with labyrinth seals impossible. VITROCELL® has developed a new holder system which is flexible to adjust to different shapes.

VITROCELL Application Note

Read more

Autor

David Thorne, British American Tobacco

Workshop series to identify, discuss and develop recommendations for the optimal generation and use of in vitro data for product regulation.

Read more

¹Leverette R, ¹Keyser B, ²Seymour A and ²Hollings M
¹Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC 27101
²Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

• Freshly generated whole smoke from the 3R4F reference cigarette (HCI; TPM) and aerosol from either a commercially available THP (HCI; glycerol) or ENDS (CRM81; glycerol) were delivered consistently within the Vitrocell® AMES 48 High Throughput exposure module.
• Coefficients of variation (CV) for whole smoke/aerosol deposition within each dose (row) were < 20% (3R4F; TPM) and < 15% (THP and ENDS; glycerol).
• Overall, the data presented demonstrate the consistent delivery of whole smoke/aerosol under controlled conditions and a reproducible in vitro biological response (Ames) with the Vitrocell® AMES 48 High Throughput exposure module. HCI 3R4F whole smoke exposures do require additional range finding experiments for optimization.
• Ames activity of 3R4F whole smoke, when generated under similar conditions, was comparable when using either the Vitrocell® AMES 48 High Throughput exposure module or the Vitrocell® Standard exposure modules (Figure 6).
• The Vitrocell® AMES 48 exposure module is a useful tool to increase sample throughput for the in vitro toxicological assessment of freshly generated whole smoke and aerosols from different tobacco product types (combustible, THP and ENDS). The AMES 48 module allows 7 smoke/aerosol doses (with 6 cultures per dose) per exposure versus only 2 (HCI) - 4 (ISO) doses (with 3 cultures per dose) for the standard exposure modules (when using a VC10® smoking machine).

Read more

https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keyser^a, Robert Leverette^a, Michael Hollings^b, Adam Seymour^b, Lesley Reeve^b, Wanda Fields^a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
^b Covance Laboratories Ltd., North Yorkshire, UK

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

Read more