DOI:10.1007/s11739-019-02055-x
Filippo Zanetti¹, Athanasios Kondylis¹, Florian Martin¹, Patrice Leroy¹, Shoaib Majeed¹, Sandro Steiner¹, Yang Xiang¹, Laura Ortega Torres¹, Keyur Trivedi¹, Emmanuel Guedj¹, Celine Merg¹, Stefan Frentzel1, Nikolai V. Ivanov¹, Utkarsh Doshi², Kyeonghee Monica Lee², Willie J. McKinneyJr², Manuel C. Peitsch¹, Julia Hoeng¹

¹ Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
² Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

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https://doi.org/10.1016/j.tiv.2018.12.010

Brian M. Keyser^a, Robert Leverette^a, Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a
a RAI Services Company1, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
^b Covance Laboratories Ltd., North Yorkshire, UK

The aims of this study were to investigate dosimetry during whole smoke exposure in the VITROCELL® VC10® smoking robot using QCMs and to determine whether photometers could be used for concurrent assessment of dose during in vitro whole smoke exposures.

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DOI: 10.1016/j.tiv.2018.06.024

Steiner S, Diana P, Dossin E, Guy P, Vuillaume G, Kondylis A, Majeed S, Frentzel S, Hoeng J

PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland.

Highlights
•Different smoke constituents are delivered with different efficiencies during in vitro exposures.
•This indicates considerable differences in the composition of the applied and the absorbed smoke.
•The composition of the applied smoke is of limited value for describing the exposure.

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https://doi.org/10.1016/j.toxrep.2018.04.003

Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a,
^a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA2
^b Covance Laboratories Ltd., North Yorkshire, UK

The article shows the successfull qualification of the Vitrocell VC10 smoking robot. The robot was used to  expose bacterial cells to cigarette whole smoke generated from 3R4F reference cigarettes and Eclipse cigarettes. A comparison was made between whole smoke from a tobacco heating and a combustible cigarette.

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DOI: 10.1089/aivt.2018.0001
Holger Behrsing,¹ Mario Aragon,¹ Jason Adamson,² Devin Sheehan,¹ Marianna Gaca,² Rodger Curren,¹ and Erin Hill^1
1Respiratory Toxicology, Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.
²R&D, British American Tobacco, Southampton, United Kingdom.

The study shows an assessment of VC1 aerosol generation that was conducted by measuring puff-by-puff in vitro nicotine concentration of 3R4F cigarettes (ISO and HCI regimes), e-cigarette (CRM81 puffing profile), and THP (HCIm regime). The aerosols were applied to in vitro or ex vivo cellular and tissue systems that model the respiratory tract, such as reconstructed human airways (RHuA), which are grown at the air–liquid interface (ALI). Additionally a comparison between the Vitrocell VC1 (U.S. Laboratory) and the VC10 (UK Laboratory) Smoking Robot was done.

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