https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keyser^a, Robert Leverette^a, Michael Hollings^b, Adam Seymour^b, Lesley Reeve^b, Wanda Fields^a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
^b Covance Laboratories Ltd., North Yorkshire, UK

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

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https://doi.org/10.1007/s00204-019-02565-9

Anita R. Iskandar, Filippo Zanetti, Diego Marescotti, Bjorn Titz, Alain Sewer, Athanasios Kondylis, Patrice Leroy, Vincenzo Belcastro, Laura Ortega Torres, Stefano Acali, Shoaib Majeed, Sandro Steiner, Keyur Trivedi, Emmanuel Guedj, Celine Merg, Thomas Schneider, Stefan Frentzel, Florian Martin, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

Previous experimental setups shows the effects of e-liquids on cell viability (first layer), followed by investigating the potential mechanisms of toxicity elicited by e-liquids (second layer) and finally assessing the impacts of aerosols (third layer). In this present work shows how the three-layer framework is leveraged to evaluate the potential toxicity and biological effects of the MESH Classic Tobacco and Base e-liquids/aerosols compared with those of 3R4F CS.

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Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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DOI:10.1007/s11739-019-02055-x
Filippo Zanetti¹, Athanasios Kondylis¹, Florian Martin¹, Patrice Leroy¹, Shoaib Majeed¹, Sandro Steiner¹, Yang Xiang¹, Laura Ortega Torres¹, Keyur Trivedi¹, Emmanuel Guedj¹, Celine Merg¹, Stefan Frentzel1, Nikolai V. Ivanov¹, Utkarsh Doshi², Kyeonghee Monica Lee², Willie J. McKinneyJr², Manuel C. Peitsch¹, Julia Hoeng¹

¹ Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
² Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

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https://doi.org/10.1016/j.tiv.2018.12.010

Brian M. Keyser^a, Robert Leverette^a, Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a
a RAI Services Company1, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
^b Covance Laboratories Ltd., North Yorkshire, UK

The aims of this study were to investigate dosimetry during whole smoke exposure in the VITROCELL® VC10® smoking robot using QCMs and to determine whether photometers could be used for concurrent assessment of dose during in vitro whole smoke exposures.

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