Advanced in vitro exposure systems.

29. Sep. 2020

Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives

Xuefei Cao1, Jayme P. Coyle2, Rui Xiong1, Yiying Wang1, Robert H. Heflich1, Baiping Ren1, William M. Gwinn3, Patrick Hayden4, Liying Rojanasakul2

1 Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Rd., AR Jefferson, USA
2 Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers forDisease Control and Prevention,Morgantown,WV, USA
3 Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Durham, NC, USA
4 BioSurfaces Inc., Ashland, MA, USA

One important element for validating any new assay for making regulatory decisions is determining its performance relative to an accepted standard. Conducting in vivo inhalation toxicity studies using whole-body or nose-only exposure systems is expensive and time-consuming and typically requires a large number of animals. The goal of using alternative methods, like human in vitro ALI airway cultures, ultimately is to replace inhalation toxicity testing in animals with in vitro approaches. Transition from animal- to human-based models is ultimately expected to lead to faster and better predictive toxicity assessments and therapeutic development at lower cost.  This study shows the development and validation of alternative in vitro methods for acute toxicity testing, including acute inhalation toxicity testing.

Read more

Comments (0) Number of views (639)

24. Mar. 2020

Comparison of Vapor and Liquid Phase Acrolein Exposures to Air Liquid Interface (ALI) Cell Cultures

David H. Brandwein, F. Adam Bettmann, Michael P. DeLorme, Alan T. Eveland, Lawrence M. Milchak 
3M Corporate Toxicology and Environmental Science, St. Paul, MN

The STL is working to develop an in vitro screening ALI model to assess the acute respiratory irritation potential for new chemicals. These experiments examined multiple aspects of the model, including different cell culture systems (A549 and EpiAirway), different exposure methods (dynamic vapor and liquid phase), and different post exposure periods, all using acrolein as a model respiratory irritant. The goal was to better understand the critical parameters of the cell systems and exposure methods to enable the development of a consistent screening model, while gaining clarity of the dosimetry. 

Read more

Comments (0) Number of views (1281)

20. Mar. 2016

VITROCELL® User Group Meeting 2016

11 March 2016, Hilton New Orleans Riverside, USA prior to the Society of Toxicology 55th Annual Meeting and ToxExpo

After a successful User Group Meeting 2015 in the EUROPA-PARK in Germany, International scientists from Europe, Japan and USA followed the invitation of VITROCELL® Systems to the 2016 User Group Meeting.

It offered an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange experiences in working with VITROCELL® devices and to meet fellow researchers. The meeting took place prior to the international Society of Toxicology 55th Annual Meeting and ToxExpo – one of the largest international conferences related to toxicology.

The focus of the event was to present new routes dosimetry, to give an update on VITROCELL’s participation in major European research programs related to nanoparticles as well as the presentation of new products for 2016.

Read more

Comments (0) Number of views (2032)

31. Aug. 2011

A novel dynamic gas mixing system to assess the human lung cells response to BTXE exposure at low levels

EUROTOX Paris, France

August 28-31, 2011; Poster #P2056

Sophie ACHARD 1, Mathilde DELAVAL 1, Tobias KREBS 2, Isabelle MOMAS 1, Nathalie SETA 1

1 Laboratory of Public Health and Environment, EA 4064, Paris Descartes University, F-75006 Paris
2 VITROCELL Systems GmbH, Waldkirch, Germany


To set up and validate the use of the Vitrocell® Spiking System (VSS), a novel gas mixing system generating low doses of VOCs, coupled to a system of exposure in air/liquid interface, to estimate the VOCs impact on the inflammatory response of airways cells.

Read more

Comments (0) Number of views (1838)

30. Jul. 2010

New on IUTOX

VITROCELL® Spiking Systems

The new tool to produce gas mixtures in the ppb range.

Read more

Comments (0) Number of views (1850)
Back to Top