Sonja Mülhopt¹, Christoph Schlager², Markus Berger², Sivakumar Murugadoss³, Peter H. Hoet³, Tobias Krebs², Hanns-Rudolf paur¹ & Dieter Stapf^1
1Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, Eggenstein-Leopoldshafen, 76344, Germany. 
²Vitrocell Systems GmbH, Waldkirch, 79183, Germany. 
³KU Leuven, Environment and Health, Leuven, 3000, Belgium.

The surface dose and the spatial distribution on the membrane delivers important data for  measuring dose-response relationships in toxicity studies.  Image evaluation of transmission electron  microscopy (TEM) samples is a highly sensitive method for determination of deposition. This paper reports  the development and characterization of a novel holder for film coated TEM copper grids, which allows for  sampling under identical geometric and ambient conditions as in a cell culture chamber. 

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https://doi.org/10.1021/acs.chemrestox.9b00489

Matthias Hufnagel, Sarah Schoch, Johanna WallBettina, Maria Strauch, Andrea Hartwig
Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, 76131 Karlsruhe, Germany

In this study an ALI exposure was combined with a high-throughput RT-qPCR approach to evaluate the toxicological potential nanoparticles in A549 cells.

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DOI: 10.1016/j.chemosphere.2019.125126 

Autors

Benjamin M King ¹ , Nathan J Janechek ¹ , Nathan Bryngelson ¹ , Andrea Adamcakova-Dodd ² , Traci Lersch ³ , Kristin Bunker ³ , Gary Casuccio ³ , Peter S Thorne ² , Charles O Stanier ⁴ , Jennifer Fiegel ⁵ 

¹Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.
²Department of Occupational and Environmental Health, The University of Iowa, 145 N. Riverside Dr., Iowa City, IA, 52242, USA.
³RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
⁴Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: charles-stanier@uiowa.edu.
⁵Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: jennifer-fiegel@uiowa.edu.

A549 Lung cells were exposed to the secondary organosilicon aerosols using the Vitrocell 6 air-liquid interface system.

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doi: 10.1016/j.tiv.2020.104798

Autors

Medina-Reyes EI¹, Delgado-Buenrostro NL², Leseman DL³, Déciga-Alcaraz A², He R4, Gremmer ER³, Fokkens PHB³, Flores-Flores JO⁵, Cassee FR⁴, Chirino YI².
¹ Laboratorio de Carcinogénesis y Toxicología, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Estado de México, Mexico. Electronic address: medinaingrid0@gmail.com.
² Laboratorio de Carcinogénesis y Toxicología, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Estado de México, Mexico.
³ National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
⁴ National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands; Institute of Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands.
⁵ Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, CP 04510 Ciudad de México, Mexico

The Air-liquid interface (ALI) model has emerged as a closer physiological system that mimics exposure in gaseous and liquid phases. This Study shows that the exposure to TiO₂ nanofibers and nanoparticles displays similar toxicity both the ALI and submerged cell cultures, using lung epithelial A549 cells. Additionally, they detected for the first time that TiO₂ nanofibers were located into the nucleus.

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DOI: 10.1016/j.chemosphere.2019.125126

Autors

King BM¹, Janechek NJ¹, Bryngelson N¹, Adamcakova-Dodd A², Lersch T³, Bunker K³, Casuccio G³, Thorne PS², Stanier CO⁴, Fiegel J⁵.

¹ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.
² Department of Occupational and Environmental Health, The University of Iowa, 145 N. Riverside Dr., Iowa City, IA, 52242, USA.
³ RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
⁴ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: charles-stanier@uiowa.edu.
⁵ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: jennifer-fiegel@uiowa.edu.

Highlights
• Oxidative flow reactor used to study effects of secondary aerosols on lung cells.
• Nanoparticulate aerosols generated from OH oxidation of D5, a cyclic siloxane.
• Acute exposures to 54–116 ng/cm² achieved using the air-liquid interface (ALI) system.
• Cytotoxic and proinflammatory effects marginal or absent at these doses.

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