Advanced in vitro exposure systems.

26. Sep. 2022

Product News 06-9/2022

VITROCELL® Cloud Alpha MOVE

For fully automated and repeated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts. Testing of up to 12 dose ranges in one experiment.

The VITROCELL® Cloud Alpha MOVE is our newest innovation and presents another great leap forward in fully automated exposure of cell cultures. It is the ideal device to obtain a dose-response relationship in one experiment.

VITROCELL® Cloud Alpha MOVE  (PDF)

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1. Jun. 2022

Product News 05-4/2022

VITROCELL® PowderX

For cell culture exposure to smallest quantities of dry powders

The VITROCELL® PowderX allows for aerosolisation of small quantities of dry powders and particle deposition on cells cultured on 12- or 24-well sized inserts. The system features biological barrier integration and continuous sampling from the basolateral compartment.

VITROCELL® PowderX  (PDF)

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15. Apr. 2022

Aerosol–Cell Exposure System Applied to Semi-Adherent Cells for Aerosolization of Lung Surfactant and Nanoparticles Followed by High Quality RNA Extraction

https://doi.org/10.3390/nano12081362


Mélanie M. Leroux 1 , Romain Hocquel 1, Kevin Bourge 1 , Boštjan Kokot 2 , Hana Kokot 2 , Tilen Kokliˇc 2, Janez Štrancar 2, Yaobo Ding 3 , Pramod Kumar 3, Otmar Schmid 3 , Bertrand H. Rihn 1, Luc Ferrari 1 and Olivier Joubert 1
1 Institut Jean Lamour, UMR CNRS 7198, Université de Lorraine, CNRS, IJL, F-54000 Nancy, France
2 Jožef Stefan Institute, Department of Condensed Matter Physics, 1000 Ljubljana, Slovenia
3 Institute of Lung Health and Immunity, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany

The goal of this work was to refine an ALI pre-established protocol for the VITROCELL® Cloud 6 system. Therefore, after addressing each crucial step, (i.e., the use of very low concentrations of NP, the deposition of a homogeneous layer of surfactant, the specific use of semi-adherent cells, and finally the enhancement of the extraction yield of RNA to carry out transcriptomic studies), we present here for the first time a robust almost ready-to-use protocol. This one will be helpful for nanotoxicologists interested in developing such methodologies.

 

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11. Apr. 2022

Product News 04-3/2022

VITROCELL® Cloud Alpha AX12

Aerosol exposure for breathing AX Lung-on-Chip

The VITROCELL® Cloud Alpha AX12 is our newest innovation in the Cloud family and presents a great leap forward in automated exposure of cell cultures with breathing function. It combines highly efficient testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12-, 24- and 96-well).
Its functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the Air/Liquid Interface have never been easier.

It is suitable for the nebulization of solutions and suspensions. Fields of application are, but are not limited to, screening of inhaled drugs and toxicity testing of inhaled substances such as chemicals, nanoparticles and airborne pathogens.

VITROCELL® Cloud Alpha AX 12  (PDF)

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6. Apr. 2022

Product News 04-2/2022

VITROCELL® Smoking Machine VC 1 2.0

Manual smoking machine with high tech features

The VC 1 smoking machine is used for a controlled aerosol generation of combustion cigarettes as well as of next generation devices such as Heated Tobacco Products (HTP) and electronic nicotine delivery systems (ENDS). Special attention was given to guarantee very flexible programming of the smoking parameters. The properties of the aerosol are maintained due to small dead volumes of the system.

VITROCELL® Smoking Machine VC 1 2.0  (PDF)

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4. Apr. 2022

Product News 04-1/2022

VITROCELL® Cloud Alpha PowerVent 

Increase of nebulizations and evacuation of residual gaseous compounds

A typical Cloud exposure allows 3 consecutive nebulizations. The PowerVent option enables an increase of the dose by adding further nebulizations. After each nebulization the humidity is removed by a short venting period before starting the new one. The venting function can be edited in the software. The PowerVent unit is delivered ready-to-use with an integrated vacuum pump.

VITROCELL® Cloud Alpha PowerVent  (PDF)

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3. Feb. 2022

Product News 02-1/2022

VITROCELL® Cloud Advanced Nebulizer

For liquid droplet sizes 9 – 12 μm with diffusor

Certain chemicals or formulations, for example nasal sprays, often have larger particle sizes than the mesh size of standard nebulizers (4.0 – 6.0 μm). The VITROCELL Cloud Advanced Nebulizer is enlarging the application areas of Cloud Exposure Systems. This special type offers the possibility to produce an aerosol containing particles in the range of 9.0 – 12.0 μm.
Our patented Cloud diffusor ensures a homogeneous distribution of even the larger droplet sizes in the Cloud Exposure Chamber. To avoid effects from larger droplet formations it has an integrated drain channel.

VITROCELL® Cloud Advanced Nebulizer  (PDF)

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1. Feb. 2022

Automation and Standardization—A Coupled Approach Towards Reproducible Sample Preparation Protocols for Nanomaterial Analysis

https://doi.org/10.3390/molecules27030985

Jörg Radnik 1, Vasile-Dan Hodoroaba 1, Harald Jungnickel 2, Jutta Tentschert 2, Andreas Luch 2, Vanessa Sogne 3, Florian Meier 3, Loïc Burr 4, David Schmid 4, Christoph Schlager 5, Tae Hyun Yoon 6,7, Ruud Peters 8, Sophie M. Briffa 9 and Eugenia Valsami-Jones 9


1 Division 6.1, Federal Institute for Material Testing and Research (BAM), Unter den Eichen 44–46, 12203 Berlin, Germany;
2 Department of Chemical & Product Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Strasse 8–10, 10589 Berlin, Germany; 
3 Postnova Analytics GmbH, Rankine-Strasse 1, 86899 Landsberg, Germany;
4 Centre Suisse d’Electronique et de Microtechnique (CSEM), Bahnhofstrasse 1, 7302 Landquart, Switzerland;
5 Vitrocell Systems GmbH, Fabrik Sonntag 3, 78193 Waldkirch, Germany; c.schlager@vitrocell.com
6 Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, Korea;
7 Institute of Next Generation Material Design, Hanyang University, Seoul 04673, Korea
8 Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 Wageningen, The Netherlands;
9 School of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UK;

 

The aim of this publication is to discuss how and when automated preparation can enhance the quality of the measurement results. For modern apparatuses, the measurement conditions are recorded and saved in the metadata automatically. As a result, the main reason for varying results is the different sample preparation. An actual interlaboratory comparison is ongoing to investigate the effect of different preparation methods systematically for ToF-SIMS. An air–liquid interface was developed to show that automation is possible for rather complex samples. Biological samples can be prepared in a reproducible manner, under exactly the same conditions on a TEM grid for the analysis of size and shape. Furthermore, chemical analysis can be performed by means of mass spectrometry.

 

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19. Jan. 2022

Product News 01/2022

VITROCELL® Photometer 2.0

Online measurement of particle concentrations

VITROCELL® Photometers are specifically designed and developed to enable online measurements of particle concen­trations. The working principe is light-scattering. They may be placed inline at various locations of the exposure system without particles losses – even at low flow rates.

VITROCELL® Photometer 2.0  (PDF)

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27. Nov. 2021

Agglomeration State of Titanium-Dioxide (TiO2) Nanomaterials Influences the Dose Deposition and Cytotoxic Responses in Human Bronchial Epithelial Cells at the Air-Liquid Interface

https://doi.org/10.3390/nano11123226

Sivakumar Murugadoss 1, Sonja Mülhopt 2, Silvia Diabaté 3, Manosij Ghosh 1, Hanns-Rudolf Paur 2,
Dieter Stapf 2, Carsten Weiss 3, and Peter H. Hoet 1,

1 Laboratory of Toxicology, Unit of Environment and Health, Department of Public Health and Primary Care, KU Leuven, 3000 Leuven, Belgium
2 Institute for Technical Chemistry, Karlsruhe Institute of Technology, 76021 Karlsruhe, Germany
3 Institute of Biological and Chemical Systems—Biological Information Processing, Karlsruhe Institute of Technology, 76021 Karlsruhe, Germany

 

In this study, they investigated the influence of agglomeration on the deposition and cytotoxic potency of TiO2 NMs at the ALI. Our results indicate that dose deposition and the cytotoxic potential are influenced by agglomeration, particularly for nano-sized TiO2 particles.

 

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