https://doi.org/10.1016/j.impact.2022.100439
Anne Bannuscher ^a, Otmar Schmid ^b,c, Barbara Drasler ^a, Alain Rohrbasser ^a, Hedwig M. Braakhuis ^d, Kirsty Meldrum ^e, Edwin P. Zwart ^d, Eric R. Gremmer ^d, Barbara Birk ^f, Manuel Rissel ^f, Robert Landsiedel ^f,g, Elisa Moschini ^h, Stephen J. Evans ^e, Pramod Kumar ^b,c, Sezer Orak ^b,c, Ali Doryab ^b,c, Johanna Samulin Erdem ⁱ, Tommaso Serchi ^h, Rob J. Vandebriel ^d, Flemming R. Cassee ^d,j, Shareen H. Doak ^e, Alke Petri-Fink ^a, Shanbeh Zienolddiny ⁱ, Martin J. D. Clift ^e, Barbara Rothen-Rutishauser ^a,
^a Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland
^b Comprehensive Pneumology Center (CPC-M), Helmholtz Zentrum München - Member of the German Center for Lung Research (DZL), Max-Lebsche-Platz 31, 81377 Munich, Germany
^c Institute of Lung Health and Immunity, Helmholtz Zentrum München – German Research Center for Environmental Health, 85764 Neuherberg, Germany
^d National Institute for Public Health and the Environment (RIVM), PO Box 1, 3720, BA, Bilthoven, the Netherlands
^e In Vitro Toxicology Group, Faculty of Medicine, Health and Life Sciences, Medical School, Institute of Life Sciences, Centre for NanoHealth, Swansea University, Singleton Campus, Wales SA2 8PP, UK
^f BASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen am Rhein, Germany
^g Free University of Berlin, Pharmacy, Pharmacology and Toxicology, 14195 Berlin, Germany.
^h Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology (LIST), 41 rue du Brill, L4422 Belvaux, Grand-Duchy of Luxembourg, Luxembourg
ⁱ National Institute of Occupational Health (STAMI), N-0033 Oslo, Norway
^j Institute for Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands

Harmonization of SOP for (nano)material aerosolization using the VITROCELL® Cloud12.
Inter-laboratory comparison among seven different laboratories in Europe.

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https://doi.org/10.1016/j.tiv.2021.105258

A. T. Juarez Facio¹, J. Yon², C. Corbière¹, T. Rogez-Florent¹, C. Castilla³, H. Lavanant³, M. Mignot³, C. Devouge-Boyer³, C. Logie¹, L. Chevalier ⁴, J.-M. Vaugeois¹, C. Monteil ^1
1 Normandie Univ, UNIROUEN, UNICAEN ABTE, 76000 Rouen, France
² Normandie Univ, UNIROUEN, INSA Rouen, CNRS, CORIA, 76000 Rouen, France.
³ Normandie Univ, INSA Rouen, UMR 6014 CNRS, COBRA, 76801, Saint Etienne Du Rouvray, France
⁴ Normandie Univ, UNIROUEN, INSA Rouen, CNRS, GPM-UMR6634, 76000 Rouen, France

Highlights

• BEAS-2B cells were successfully exposed at ALI to primary UFPs emissions
• PAHs-rich UFPs induced an acute cellular toxicity at low exposed dose
• PAHs-rich UFPs activated oxidative stress and xenobiotic metabolism
• This methodological approach offers new perspectives to evaluate different kinds of UFPs

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For fully automated and repeated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts. Testing of up to 12 dose ranges in one experiment.

The VITROCELL® Cloud Alpha MOVE is our newest innovation and presents another great leap forward in fully automated exposure of cell cultures. It is the ideal device to obtain a dose-response relationship in one experiment.

VITROCELL® Cloud Alpha MOVE  (PDF)

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https://doi.org/10.3390/nano12152609

Laura Maria Azzurra Camassa ¹, Elisabeth Elje ^2,3, Espen Mariussen ^2,4, Eleonora Marta Longhin ², Maria Dusinska ², Shan Zienolddiny-Narui ¹, and Elise Rundén-Pran ^2
1 National Institute of Occupational Health in Norway, 0033 Oslo, Norway
² NILU—Norwegian Institute for Air Research, 2027 Kjeller, Norway
³ Institute of Basic Medical Sciences, Department of Molecular Medicine, University of Oslo, 0372 Oslo, Norway
⁴ Norwegian Institute of Public Health, FHI, 0456 Oslo, Norway
 

This study aimed at testing the robustness and sensitivity, characterizing, and optimizing an advanced respiratory model built on human alveolar epithelial A549 cells and evaluating its response to aerosol exposure of silver NM-300K. In more detail, we compared the responses of A549 cells cultured at the ALI in the VITROCELL® 6 Cloud system in monoculture, in coculture with EA.hy926 cells, and in triculture with EA.hy926 cells and dTHP-1, and performed an interlaboratory trial across two laboratories.

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https://doi.org/10.3390/nano12081362

Mélanie M. Leroux ¹ , Romain Hocquel ¹, Kevin Bourge ¹ , Boštjan Kokot ² , Hana Kokot ² , Tilen Koklič ², Janez Štrancar ², Yaobo Ding ³ , Pramod Kumar ³, Otmar Schmid ³ , Bertrand H. Rihn ¹, Luc Ferrari ¹ and Olivier Joubert ^1
1 Institut Jean Lamour, UMR CNRS 7198, Université de Lorraine, CNRS, IJL, F-54000 Nancy, France;
² Jožef Stefan Institute, Department of Condensed Matter Physics, 1000 Ljubljana, Slovenia;
³ Institute of Lung Health and Immunity, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany;

This study reports the optimization of an innovative air–liquid interface system using a cloud settling exposure system by VITROCELL^® Systems for pre-coating of the cells with surfactant lung lining fluid and subsequent NP exposure, combined with an accurate micrometric balance to measure cell exposure in real-time.

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