Advanced in vitro exposure systems.

27. Jun. 2021

Impact of Nanocomposite Combustion Aerosols on A549 Cells and a 3D Airway Model

https://doi.org/10.3390/nano11071685

Matthias Hufnagel 1, Nadine May 2, Johanna Wall 1, Nadja Wingert 3, Manuel Garcia-Käufer 3, Ali Arif 3, Christof Hübner 4, Markus Berger 5, Sonja Mülhopt 2, Werner Baumann 2, Frederik Weis 6, Tobias Krebs 5, Wolfgang Becker 4, Richard Gminski 3, Dieter Stapf 2, and Andrea Hartwig 1,


1 Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany;
2 Institute for Technical Chemistry, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany; 
3 Institute for Infection Prevention and Hospital Epidemiology, Medical Center—University of Freiburg, Faculty of Medicine, University of Freiburg, 79110 Freiburg, Germany;
4 Fraunhofer Institute of Chemical Technology, 76327 Pfinztal, Germany; 
5 Vitrocell® Systems GmbH, 79183 Waldkirch, Germany; 
6 Palas GmbH, 76229 Karlsruhe, Germany; 

 

This study was the first to investigate the toxicological effects of well characterized aerosols released during combustion of thermoplastic nanocomposites using an air–liquid interface exposure system. Even though studies on the toxicological potential of combustion-generated particulate matter as well as VOCs have been published, none of them was designed to investigate the effect of the native aerosol using appropriate realistic lung cell culture models. In the current study we investigated the combustion behavior of PE-based nanocomposites on a lab-scale burner. As nanoscaled fillers TiO2 NP, CuO NP, as well as CNT were chosen for this study, with TiO2 NP representing a commonly used insoluble and inert nanomaterial, CuO NP as a known in vitro cyto- as well as genotoxic nanomaterial, and CNT as a fiber-shaped nanomaterial.

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21. Jun. 2021

In vitro hazard characterization of simulated aircraft cabin bleed-air contamination in lung models using an air-liquid interface (ALI) exposure system

https://doi.org/10.1016/j.envint.2021.106718

Rui-Wen He a,b, Marc M.G. Houtzager c, W.P. Jongeneel a, Remco H.S. Westerink b, Flemming R. Cassee a,b

a National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, the Netherlands
b Institute for Risk Assessment Sciences (IRAS), Toxicology Division, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80177, 3508 TD Utrecht, the Netherlands
c The Netherlands Organisation for Applied Scientific Research, TNO, P.O. Box 80015, 3508 TA Utrecht, the Netherlands

 

This unique experimental “Mini-BACS + AES” setup is able to provide steady conditions to perform in vitro exposure under ALI conditions to aircraft engine oil and hydraulic fluid fumes, generated at respectively 350 ◦C and 200 ◦C. Exposure of the Calu-3 monoculture and Calu-3 + MDM co-culture lung cell models to high levels of aircraft engine oil and hydraulic fluid fumes under ALI conditions can reduce TEER and viabilities of the cells, induce cytotoxicity, and increase production of proinflammatory cytokines. Hydraulic fluid fumes are more toxic than engine oil fumes on the mass concentration of fume basis, which may be related to higher abundance of OPs and smaller particle size of hydraulic fluid fumes. The toxicological data clearly reflect the potential health risks during fume events in aircraft cabins.

 

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19. Apr. 2021

Product News 04/2021

The new VITROCELL® Millicell Holder

How to use Millicell 24-well Standing Inserts in the VITROCELL® 12 Module Series

The revolutionary new holder system enables for the use of standing inserts in all modules of the VITROCELL® 12 Series for 12-well sized inserts. As an additional advantage, the media com­partment is sealed towards the aerosol exposure head to avoid contact of the test substance with the cell culture media.

VITROCELL® Millicell Holder – How to use Millicell 24-well Standing inserts in the VITROCELL® 12 Module Series

 

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3. Mar. 2021

Vitrocell® Leak Tester

Must-have device for aerosol research – easy and efficient leak detection

Regular leak tests are mandatory in aerosol research. A leak caused by a forgotten connection or defective o-ring may have a significant influence on the aerosol exposure process. We recommend to carry out a leak test prior to the experiment and as part of cleaning or service routine.

VITROCELL Application Note

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23. Feb. 2021

Product News 02/2021

The New VITROCELL® RH/T-Controller

Reliable digital Humidity- and Temperature Measurement

The VITROCELL® RH/T-Controller system was designed to measure temperature and relative humidity in numerous applications at high precision. It can be fitted to humidification systems, aerosol ducts and climatic chambers.

VITROCELL® RH/T-Controller – Reliable digital Humidity- and Temperature Measurement

 

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15. Feb. 2021

Impact of Sea Breeze Dynamics on Atmospheric Pollutants and Their Toxicity in Industrial and Urban Coastal Environments

doi:10.3390/rs12040648

Patrick Augustin 1, Sylvain Billet 2 , Suzanne Crumeyrolle 3, Karine Deboudt 1, Elsa Dieudonné 1 , Pascal Flament 1 , Marc Fourmentin 1 , Sarah Guilbaud 1, Benjamin Hanoune 4 , Yann Landkocz 2, Clémence Méausoone 2, Sayahnya Roy 5, François G. Schmitt 5, Alexei Sentchev 5 and Anton Sokolov 1
1 Univ. Littoral Côte d’Opale, UR 4493—LPCA—Laboratoire de Physico-Chimie de l’Atmosphère, 59140 Dunkerque, France;
2 Univ. Littoral Côte d’Opale, SFR Condorcet FR CNRS 3417, UR 4492—UCEiV—Unité de Chimie Environnementale et Interactions sur le Vivant, 59140 Dunkerque, France; 
3 Univ. Lille, CNRS, UMR 8518—LOA—Laboratoire d’Optique Atmosphérique, 59000 Lille, France;
4 Univ. Lille, CNRS, UMR 8522—PC2A—Physico-Chimie des Processus de Combustion et de l’Atmosphère, 59000 Lille, France; 
5 Univ. Lille, Univ. Littoral Côte d’Opale, CNRS, UMR 8187—LOG—Laboratoire d’Océanologie et de Géosciences, F 62930 Wimereux, France; 

 

An atmospheric mobile unit was implemented during a field campaign performed at a representative site of urbanized and industrialized coastal environment of the North Sea (Northern France), to study the impact of sea breeze dynamics on aerosol properties, and especially their toxicity. This unit combined aerosol samplers, two scanning lidars (Doppler and elastic), two aerosol particle sizers and an air-liquid interface (ALI, Vitrocell) in vitro cell exposure device. This study is one of the first to bring cell cultures into the field to evaluate the harmfulness of a real environmental compartment. Atmospheric toxicity in the presence or absence of sea breeze was demonstrated in human bronchial cells exposed in the field using an ALI exposure system. The study showed the sensitivity of the developed device to discriminate the mechanisms of toxic action activated when exposed to different atmospheres.

 

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22. Jan. 2021

Product News 01/2021

The New VITROCELL® Cloud Alpha MAX

Smallest nebulization volumes and high deposition efficiency

The VITROCELL® Cloud Alpha MAX is designed for small nebulization volumes and very high deposition efficiency. This is important when only small quantities of material are available or when expensive test substances need to be tested.

VITROCELL® Cloud Alpha MAX – Ideal for small quantities of test substance

 

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15. Jan. 2021

New Video: VITROCELL Cloud Alpha – Product Family Overview

For virus research, testing of inhaled drugs, chemicals and nanoparticles at the Air/Liquid Interface (ALI). The systems are suitable for nebulized solutions and suspensions.

The VITROCELL® Cloud Alpha series is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines reliable exposure of cell cultures from the respiratory tract with ease of use. The series comprises the Cloud Alpha 6, Cloud Alpha 12, Cloud Alpha 96 and Cloud Alpha MAX – they represent an optimal solution for everyday experiments at the Air/Liquid Interface using 6-well, 12-well, 24-well or 96-sized inserts.

This video showcases available product configurations.

 

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29. Dec. 2020

Air–Liquid Interface Exposure of Lung Epithelial Cells to Low Doses of Nanoparticles to Assess Pulmonary Adverse Effects

https://doi.org/10.3390/nano11010065

 

Silvia Diabaté 1, Lucie Armand 2, Sivakumar Murugadoss 1 , Marco Dilger 1 , Susanne Fritsch-Decker 1, Christoph Schlager 3, David Béal 2, Marie-Edith Arnal 2, Mathilde Biola-Clier 2, Selina Ambrose 4, Sonja Mülhopt 3, Hanns-Rudolf Paur 3, Iseult Lynch 5 , Eugenia Valsami-Jones 5 , Marie Carriere 2, and Carsten Weiss 1

1 Karlsruhe Institute of Technology, Institute of Biological and Chemical Systems–Biological Information Processing, 76344 Eggenstein-Leopoldshafen, Germany;
2 CEA, CNRS, IRIG, SyMMES, University Grenoble Alpes, 38054 Grenoble, France;
3 Karlsruhe Institute of Technology, Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, Germany; 
4 Promethean Particles Ltd., Nottingham NG7 3EF, UK;
5 School of Geography Earth & Environmental Sciences (GEES), University of Birmingham (UoB), Edgbaston, Birmingham B15 2TT, UK;

 

KIT, together with VITROCELL SYSTEMS, set up a first Automated Exposure Station, which has been used for the assessment of nanoscale particle emissions from combustion sources such as ship diesel and wood burners. The system was further developed and offers a compact solution for toxicity testing of nanoparticle (NP) aerosols including sample conditioning, reproducible deposition, integrated dose determination by a quartz crystal microbalance (QCM), flow control, automated processes and data acquisition. The device was also tested with partner laboratories with the aim of potentially standardizing and achieving regulatory acceptance of the method.

 

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30. Nov. 2020

Product News 12/2020

VITROCELL® 12/12 now available with Climatic Chamber

For reliable aerosol exposure of cell cultures using 12- or 24-well sized inserts

The VITROCELL® 12/12 module system has been designed to facilitate the exposure of mammalian cell cultures to airborne substances such as gases, complex mixtures, nanoparticles and fibers. It features a throughput of 3 dilution steps @ 3 replicates and 1 clean air control at 3 replicates. In order to optimize the exposure for liquid aerosols and to maximize the performance of humidified air supply it is now also available in a user-friendly climatic chamber.

VITROCELL® 12/12 now available with Climatic Chamber

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