Vitrocell Systems GmbH - nanoparticles

26. Jul. 2018

Hybrid Lipid/Polymer Nanoparticles for Pulmonary Delivery of siRNA: Development and Fate Upon In Vitro Deposition on the Human Epithelial Airway Barrier

DOI: 10.1089/jamp.2017.1364

Ivana d’Angelo, PhD,¹ Gabriella Costabile, PhD,^2,3 Estelle Durantie, PhD,³ Paola Brocca, PhD,⁴ Valeria Rondelli, PhD,⁴ Annapina Russo, PhD,⁵ Giulia Russo, PhD,⁵ Agnese Miro, PharmD,² Fabiana Quaglia, PhD,² Alke Petri-Fink, PhD,³ Barbara Rothen-Rutishauser, PhD,³ and Francesca Ungaro, PhD²
¹ Di.S.T.A.Bi.F., University of Campania ‘‘Luigi Vanvitelli,’’ Caserta, Italy.
²Laboratory of Drug Delivery, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.
³ Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
⁴ Applied Physics, Department of Medical Biotechnology and Translational Medicine, University of Milano, Milano, Italy.
⁵ Laboratory of Biochemistry, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.

The Vitrocell Cloud was used for the exposure to aerosolized nanoparticles on a in vitro three-dimensional cell cluture, mimicking the human epithelial airway barrier comprising human bronchial epithelial cells,human blood monocyte-derived macrophages and dendritic cells to show the optimized siRNA transport and delivery at lung.

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Categories: Publications

Tags: nanoparticles, VITROCELL Cloud, aerosol generator, air-liquid Interface

15. Jun. 2018

ACE nano: pursuing analytical and characterisation excellence in nanomaterial risk assessment

Nanomaterials are man-made materials of a size thousands of times smaller than the width of a human hair. They have fascinated scientists and industry with their unique and unpredictable properties, which have given rise to an endless variety of new applications in every sector of technology and medicine. As a result, an ever-increasing number of nanomaterials are entering the market in everyday products spanning from healthcare and leisure to electronics, cosmetics and foodstuffs. However, the novelty in exploitable properties may be mirrored by new hazards and, in order to manage these, a well-founded and robust legislative framework that will ensure safe development of nano-enabled products is needed.

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Categories: Product News

Tags: nanoparticles

6. Jun. 2018

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

DOI: 10.2217/nnm-2017-0358

Savvina Chortarea^1,2, Kleanthis Fytianos^1,3,4, Laura Rodriguez-Lorenzo¹, Alke Petri-Fink^1,5 & Barbara Rothen-Rutishauser¹
¹BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
²Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
³Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
⁴Department of Clinical Research, University of Bern, Bern, Switzerland
⁵Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, microbalance, liquid aerosol, VITROCELL Cloud, air-liquid Interface, airborne particles

1. Jun. 2018

Charakterisierung von Partikelemissionen aus dem Trennschleifprozess von kohlefaserverstärktem Beton (Carbonbeton)

Autoren:
Hillemann, L.; Stintz, M.; Streibel, T.; Zimmermann, R.; Öder, S.; Kasurinen, S.; di Bucchianico, S.; Kanashova, T.; Dittmar, G.; Konzack, D.; Große, S.; Rudolph, A.; Berger, M.; Krebs, T.; Saraji-Bozorgzad, M. R.; Walte, A.

Die Wirkung von Emissionen aus dem Trennen von Carbonbeton werden in einer Kombination aus Trennstaubgenerator, Verdünnungssystem und Expositionssystem an Zellkulturen (A549 und THP-1) gegenüber den entstehenden Stäuben bestimmt (Zytotoxizität).

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Categories: Publications

Tags: inhalation exposure, nanoparticles, exposure station, air-liquid Interface, airborne particles

26. May. 2018

Acute effects of multi-walled carbon nanotubes on primary bronchial epithelial cells from COPD patients

DOI: 10.1080/17435390.2018.1472310

Beyeler S^1,2, Chortarea S^3,4, Rothen-Rutishauser B³, Petri-Fink A³, Wick P⁴, Tschanz SA⁵, von Garnier C^1,2, Blank F^1,2.

¹ Department of BioMedical Research , University of Bern , Bern , Switzerland.
² Department of Pulmonary Medicine , University Hospital of Bern , Bern , Switzerland.
³ BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg , Switzerland.
⁴ Laboratory for Particles-Biology Interactions, Empa Swiss Federal Laboratories for Material Science and Technology , St. Gallen , Switzerland.
⁵Institute of Anatomy, University of Bern , Bern, Switzerland.

This paper shows the investigation of acute effects of occupational multi-walled carbon nanotubes exposure in primary epithelial lung cell cultures from potentially susceptible individuals with COPD.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, microbalance, liquid aerosol, VITROCELL Cloud, air-liquid Interface, airborne particles

11. Mar. 2018

Update: Deposition Enhancement via Thermophoresis

User Group Meeting 2018

Dr. Dirk Brossell

Federal Institute for Occupational Safety and Health (BAuA), Unit 4.5, 10317 Berlin, Germany

The poster demonstrates the particle deposition via thermal precipitation (particle precipitation by thermophoresis) on cells hosted at the air-liquid interface (ALI).
Advantages of thermophoresis
• Diffusion-like mechanism, gentle particle deposition
• Independent from particle size (for nanoparticles)
• Homogenous deposition density profile
• No need to manipulate aerosol properties for deposition (charge, size)

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Categories: Publications

Tags: inhalation exposure, nanoparticles, VITROCELL 6, air-liquid Interface, airborne particles

11. Mar. 2018

VITROCELL® Powder Chamber

User Group Meeting 2018

Katharina Knoth¹, Marius Hittinger¹, Tobias Krebs²

¹PharmBioTec GmbH, Science Park 1, 66123 Saarbrücken, Germany

²VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The poster shows two customer examples for exposures to smallest quantities of dry powders.

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Categories: Publications

Tags: nanoparticles, air-liquid Interface, airborne particles, powder chamber

11. Mar. 2018

New Products at VITROCELL® User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2018.

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11. Mar. 2018

Elucidating the impact of nanomaterials on genomic stability using high-throughput RT-qPCR and Alkaline Unwinding

User Group Meeting 2018

Matthias Hufnagel, Prof. Dr. Andrea Hartwig

KIT - Karsruhe Institute of Technology

The poster shows the establisment of the use of a high-throughput RT-qPCR method and the quantitative detection of DNA strand breaks (Alkaline Unwinding) for cells exposed in the Air-Liquid Interface.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, microbalance, liquid aerosol, VITROCELL Cloud

29. Nov. 2017

Biodistribution of single and aggregated gold nanoparticles exposed to the human lung epithelial tissue barrier at the air-liquid interface

DOI: 10.1186/s12989-017-0231-3

Estelle Durantie¹, Dimitri Vanhecke¹, Laura Rodriguez-Lorenzo¹, Flavien Delhaes¹, Sandor Balog¹, Dedy Septiadi¹, Joel Bourquin¹, Alke Petri-Fink^1,2 and Barbara Rothen-Rutishauser¹

¹BioNanomaterials Group, Adolphe Merkle Institute, Université de Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.

²Chemistry Department, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

In this study, the biodistribution of aerosolized single and aggregated nanoparticles was investigated using a 3D model of the human epithelial tissue barrier. The cells were exposed at the air-liquid interface using the Vitrocell® Cloud exposure system. It consists of three main parts: a nebulizer, an aerosol chamber and a base module constituted of 12-well size inserts and connected to a controlled heating unit. Robust characterization was used to evaluate the exact delivered dose onto the cell surface and to determine the cellular uptake and translocation across the barrier.

Comments (0) Number of views (230)

Categories: Publications

Tags: inhalation exposure, nanoparticles, liquid aerosol, VITROCELL Cloud, air-liquid Interface, airborne particles

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User Group Meeting 2018

User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

User Group Meeting 2018

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