Advanced in vitro exposure systems.

15. Jun. 2018

ACE nano: pursuing analytical and characterisation excellence in nanomaterial risk assessment

Nanomaterials are man-made materials of a size thousands of times smaller than the width of a human hair. They have fascinated scientists and industry with their unique and unpredictable properties, which have given rise to an endless variety of new applications in every sector of technology and medicine. As a result, an ever-increasing number of nanomaterials are entering the market in everyday products spanning from healthcare and leisure to electronics, cosmetics and foodstuffs. However, the novelty in exploitable properties may be mirrored by new hazards and, in order to manage these, a well-founded and robust legislative framework that will ensure safe development of nano-enabled products is needed.

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Categories: Product News

Tags: nanoparticles

6. Jun. 2018

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

DOI: 10.2217/nnm-2017-0358

Savvina Chortarea1,2, Kleanthis Fytianos1,3,4, Laura Rodriguez-Lorenzo1, Alke Petri-Fink1,5 & Barbara Rothen-Rutishauser1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
2Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
3Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
4Department of Clinical Research, University of Bern, Bern, Switzerland
5Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

 

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1. Jun. 2018

Charakterisierung von Partikelemissionen aus dem Trennschleifprozess von kohlefaserverstärktem Beton (Carbonbeton)

Autoren:
Hillemann, L.; Stintz, M.; Streibel, T.; Zimmermann, R.; Öder, S.; Kasurinen, S.; di Bucchianico, S.; Kanashova, T.; Dittmar, G.; Konzack, D.; Große, S.; Rudolph, A.; Berger, M.; Krebs, T.; Saraji-Bozorgzad, M. R.; Walte, A.

Die Wirkung von Emissionen aus dem Trennen von Carbonbeton werden in einer Kombination aus Trennstaubgenerator, Verdünnungssystem und Expositionssystem an Zellkulturen (A549 und THP-1) gegenüber den entstehenden Stäuben bestimmt (Zytotoxizität).

 

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26. May. 2018

Acute effects of multi-walled carbon nanotubes on primary bronchial epithelial cells from COPD patients

DOI: 10.1080/17435390.2018.1472310

Beyeler S1,2, Chortarea S3,4, Rothen-Rutishauser B3, Petri-Fink A3, Wick P4, Tschanz SA5, von Garnier C1,2, Blank F1,2.

1 Department of BioMedical Research , University of Bern , Bern , Switzerland.
2 Department of Pulmonary Medicine , University Hospital of Bern , Bern , Switzerland.
3 BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg , Switzerland.
4 Laboratory for Particles-Biology Interactions, Empa Swiss Federal Laboratories for Material Science and Technology , St. Gallen , Switzerland.
5 Institute of Anatomy, University of Bern , Bern, Switzerland.

This paper shows the investigation of acute effects of occupational multi-walled carbon nanotubes exposure in primary epithelial lung cell cultures from potentially susceptible individuals with COPD.

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11. Mar. 2018

Update: Deposition Enhancement via Thermophoresis

User Group Meeting 2018

Dr. Dirk Brogell

Federal Institute for Occupational Safety and Health (BAuA), Unit 4.5, 10317 Berlin, Germany

The poster demonstrates the particle deposition via thermal precipitation (particle precipitation by thermophoresis) on cells hosted at the air-liquid interface (ALI).
Advantages of thermophoresis
• Diffusion-like mechanism, gentle particle deposition 
• Independent from particle size (for nanoparticles)
• Homogenous deposition density profile 
• No need to manipulate aerosol properties for deposition (charge, size)

 

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11. Mar. 2018

VITROCELL® Powder Chamber

User Group Meeting 2018

Katharina Knoth1, Marius Hittinger1, Tobias Krebs2

1PharmBioTec GmbH, Science Park 1, 66123 Saarbrücken, Germany  

2VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The poster shows two customer examples for exposures to smallest quantities of dry powders.

 

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11. Mar. 2018

New Products at VITROCELL® User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2018.

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11. Mar. 2018

Elucidating the impact of nanomaterials on genomic stability using high-throughput RT-qPCR and Alkaline Unwinding

User Group Meeting 2018

Matthias Hufnagel, Prof. Dr. Andrea Hartwig 

KIT - Karsruhe Institute of Technology

The poster shows the establisment of the use of a high-throughput RT-qPCR method and the quantitative detection of DNA strand breaks (Alkaline Unwinding) for cells exposed in the Air-Liquid Interface.

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29. Nov. 2017

Biodistribution of single and aggregated gold nanoparticles exposed to the human lung epithelial tissue barrier at the air-liquid interface

DOI: 10.1186/s12989-017-0231-3

Estelle Durantie1, Dimitri Vanhecke1, Laura Rodriguez-Lorenzo1, Flavien Delhaes1, Sandor Balog1, Dedy Septiadi1, Joel Bourquin1Alke Petri-Fink1,2 and Barbara Rothen-Rutishauser1

1BioNanomaterials Group, Adolphe Merkle Institute, Université de Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland. 

2Chemistry Department, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.

 

In this study, the biodistribution of aerosolized single and aggregated nanoparticles was investigated using a 3D model of the human epithelial tissue barrier.  The cells were exposed at the air-liquid interface using the Vitrocell® Cloud exposure system. It consists of three main parts: a nebulizer, an aerosol chamber and a base module constituted of 12-well size inserts and connected to a controlled heating unit. Robust characterization was used to evaluate the exact delivered dose onto the cell surface and to determine the cellular uptake and translocation across the barrier.


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15. May. 2017

Human Asthmatic Bronchial Cells Are More Susceptible to Subchronic Repeated Exposures of Aerosolized Carbon Nanotubes At Occupationally Relevant Doses Than Healthy Cells

DOI: 10.1021/acsnano.7b01992

Savvina Chortarea,1 Hana Barosova,1 Martin James David Clift,2 Peter Wick,3 Alke Petri-Fink,1,4 and Barbara Rothen-Rutishauser,1

1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, CH-1700 Fribourg, Switzerland
2In Vitro Toxicology Group, Swansea University Medical School, Swansea SA2 8PP, Wales, U.K.
3Laboratory for Materials-Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science and Technology, 9014 St Gallen, Switzerland
4Department of Chemistry, University of Fribourg, CH-1700 Fribourg, Switzerland

The aim of this study was to realistically mimic subchronic inhalation of multiwalled carbone nanotubes in vitro, using the air−liquid interface cell exposure system for aerosol exposures on reconstituted human bronchial tissue from healthy and asthmatic donors. Cytotoxicity, cell morphology, cilia beating frequency, mucociliary clearance, gene expression analysis of oxidative stress and (pro‑)inflammatory markers as well as (pro‑)inflammatory cytokine secretion were successfully observed.

 

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