Advanced in vitro exposure systems.

14. Aug. 2016

Copper oxide nanoparticles: Impact on alveolar epithelial-like cells following air-liquid interface exposure

EEMGS Annual Meeting: 14 – 18 August 2016, Copenhagen, Denmark

doi:10.1007/s00204-015-1621-7

Matthias Hufnagel, Sarah Schoch, Bettina Fischer, Andrea Hartwig
Karlsruhe Institute of Technology, Institute of Applied Biosciences, Department of Food Chemistry and Toxicology

This is work is part of ProCycle (No. 03XP0009), a project funded by the German Federal Ministry of Education and Research.and shows the exposition of copper oxide (CuO) nanoparticles on A549 cells at different doses in the VITROCELL® Cloud, equipped with a quartz crystal microbalance. The effects were determined using the colony formation assay (CFA) and high-throughput RT-qPCR gene expression analyses.

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27. Jun. 2016

Metabolic Profiling as Well as Stable Isotope Assisted Metabolic and Proteomic Analysis of RAW 264.7 Macrophages Exposed to Ship Engine Aerosol Emissions

Different Effects of Heavy Fuel Oil and Refined Diesel Fuel

DOI:10.1371/journal.pone.0157964

Sean C. Sapcariu1,12, Tamara Kanashova2,12, Marco Dilger3,4,12, Silvia Diabaté3,12, Sebastian Oeder5,6,12,13, Johannes Passig7,12, Christian Radischat7,12, Jeroen Buters5,6,12,13, Olli Sippula8,12, Thorsten Streibel7,9,12, Hanns-Rudolf Paur4,12, Christoph Schlager4,12, Sonja Mülhopt4,12, Benjamin Stengel10,12, Rom Rabe10,12, Horst Harndorf10,12, Tobias Krebs11,12, Erwin Karg9, Thomas Gröger9, Carsten Weiss3,12, Gunnar Dittmar2,12, Karsten Hiller1,12, Ralf Zimmermann7,9,12

1 Luxembourg Centre for Systems Biomedicine 6, avenue du Swing, L-4362 Esch-sur-Alzette, Luxembourg,
2 Mass Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine Berlin-Buch, Berlin, Germany,
3 Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
4 Institute for Technical Chemistry (ITC), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
5 Center of Allergy and Environment (ZAUM), Helmholtz Zentrum München and Technische Universität München, Munich, Germany,
6 CK-CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland,
7 Joint Mass Spectrometry Centre, Division of Analytical and Technical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany,
8 University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland,
9 Joint Mass Spectrometry Centre, CMA – Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany,
10 Chair of Piston Machines and Internal Combustion Engines, University Rostock, Rostock, Germany,
11 Vitrocell GmbH, Waldkirch, Germany,
12 HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany; Kuopio, Finland; Cardiff, United Kingdom; Esch-Belval, Luxembourg,
13 German Center for Lung Research (DZL), Munich, Germany

An automated ALI exposure system station with 18 exposure positions was used as the interface for mouse macrophage RAW 264.7 cell line exposures of the diesel engine exhaust. The evaluation is devided in LDH release assay, Metabolite extraction and GC-MS processing, Stable isotope labeling by amino acids in cell culture (SILAC), Proteome extraction and LC-MS/MS analysis of peptides and Proteomics Data An

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16. Jun. 2016

Nanoparticles on human lung cells

The Vitrocell Exposure System for cell cultures at the air-liquid interface

Sonja Mülhopt1, Tobias Krebs2, Dr Silvia Diabaté3, Christoph Schlager1, Dr Hanns-Rudolf Paur1

1 Institute for Technical Chemistry, Karlsruhe Institute of Technology, 
2 Vitrocell Systems GmbH, 
3 Institute of Toxicology and Genetics, Karlsruhe Institute of Technology

 

This article shows the development of an automated exposure system which allows both reproducible sampling and conditioning of aerosols and exposure of the cell cultures under conditions imitating those of the human lung. Bioassays were developed and used for toxicological analysis of particulate emissions from the industry as well as of nanoparticles.

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15. Mar. 2016

A Proposed In Vitro Method to Assess Effects of Inhaled Particles on Lung Surfactant Function

DOI: 10.1165/rcmb.2015-0294MA

Jorid B. Sørli1, Emilie Da Silva1, Per Bäckman2, Marcus Levin1, Birthe L. Thomsen1, Ismo K. Koponen1, and Søren T. Larsen1
1The National Research Centre for the Working Environment, Copenhagen, Denmark
2AstraZeneca, Mölndal, Sweden

This study has demonstrated that the modified CDS, a novel method that mimics the conditions for the lung surfactant, is a useful bioassay to assess the acute effects of dry powder aerosols on the lung. The in vitro method, based on the constrained drop surfactometer, different Turbuhaler as well as empty Turbuhaler filled with albumin or lactose were tested. A quartz crystal microbalance was fitted in the chamber to estimate the dose of the deposited particles.


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9. Mar. 2016

Toxicity testing of combustion aerosols at the air-liquid interface with a self-contained and easy-to-use exposure system

doi:10.1016/j.jaerosci.2016.02.005

Sonja Mülhopt a, i, Marco Dilger a, b, i, Silvia Diabaté b, i, Christoph Schlager a, i, Tobias Krebs c, i, Ralf Zimmermann d, h, i, Jeroen Buters e, i, Sebastian Oeder e, g, i, Thomas Wäscher f, Carsten Weiss b, i, Hanns-Rudolf Paur a, i

a Karlsruhe Institute of Technology, Institute for Technical Chemistry, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
b Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
c Vitrocell Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany
d University of Rostock, Institute of Chemistry, Dr.-Lorenz-Weg 1, 18051 Rostock, Germany
e Center of Allergy & Environment (ZAUM), Technische Universität and Helmholtz Zentrum München, Biedersteiner Str. 29, 80802 München, Germany
f Ingenieurbüro für Energie- und Verfahrenstechnik, Von-Dalheim-Str. 2, 69231 Rauenberg, Germany
g Kühne Foundation, Christine Kühne Center for Allergy Research and Education (CK- CARE), München, Germany
h Cooperation Group “Comprehensive Molecular Analytics” – CMA, Helmholtz Zentrum München, 85764 Oberschleißheim/ Germany
i HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health

 

Highlights

• We present a fully automated Air-Liquid-Interface Exposure System.
• System characterisation shows a high reproducibility.
• Wood combustion aerosol causes cytotoxicity in human lung epithelial cells.
• Gene regulation caused by ship diesel emissions in human lung epithelial cells.

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30. Oct. 2014

An inter-machine comparison of tobacco smoke particle deposition in vitro from six independent smoke exposure systems

Toxicology in Vitro

Volume 28, Issue 7, October 2014, Pages 1320-1328 (doi: 10.1016/j.tiv.2014.06.012)

Authors
J. Adamsona, D. Thornea, G. Erringtona, W. Fieldsb, X. Lic, , R. Payned, T. Krebse, A. Dalrymplea, K. Fowlerb, D. Dillona, F. Xiec, , C. Mereditha

a British American Tobacco, Group R&D, Southampton SO15 8TL, UK
b R.J. Reynolds Tobacco Co., P.O. Box 1487, Winston-Salem, NC 27102, USA
c Zhengzhou Tobacco Research Institute of China National Tobacco Corporation,
No.2 Fengyang Street, High-Tech Zone, Zhengzhou, PR China
d Covance Laboratories Ltd., Otley Road, Harrogate HG3 1PY, UK
e Vitrocell® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany

 

Comparison of different smoke exposure systems to demonstrate VC 10® suitability for tobacco smoke exposure in vitro. The results are shown in the particle deposition across the modules.

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15. Jul. 2014

A method for assessment of the genotoxicity of mainstream cigarette-smoke by use of the bacterial reverse-mutation assay and an aerosol-based exposure system

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

Volume 769, 15 July 2014, Pages 20–28, [epub ahead of print]

Authors
Joanne Kilforda, David Thorneb, Rebecca Paynea, Annette Dalrympleb, Julie Clementsa, Clive Meredithb, Debbie Dillonb

a Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK
b British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK

Successfull modifikation of the Ames assay for the testing of a mainstream cigarette smoke aerosol that is generated with a VC 10® exposure system. The reproducebility of the response on a second VC 10® system is shown.

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17. Jun. 2014

Development of a BALB/c 3T3 neutral red uptake cytotoxicity test using a mainstream cigarette smoke exposure system

BioMed Central

BMC Research Notes 2014, 7:367 (doi:10.1186/1756-0500-7-367)

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Linsey Haswell1, Annette Dalrymple1, Clive Meredith1 and Deborah Dillon1

1 British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

Mainstream cigarette smoke and the gase vapour phase, generated with the VC 10® smoke exposure system, indicates significant differently in-vitro cytotoxicity profiles. The used method on the air-liquid interface takes into account the synergies of both the particulate and vapour phase of tobacco smoke in the NRU assay.

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31. May. 2014

New VITROCELL® Automated Exposure Station delivered to NanoMile consortium

The NanoMILE project intends to establish a fundamental understanding of the mechanisms of nanomaterial interactions with living systems and the environment. It consists of 28 reseach partners. VITROCELL supllies the ALI technology.

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20. May. 2014

VITROCELL User Group Meeting

Meeting featuring the new VITROCELL® Automated Exposure Station

International scientists from Europe, Japan, Korea and USA followed the invitation of VITROCELL® Systems to the 2014 User Group Meeting in the Europa-Park Rust, Germany.

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