S. Steiner¹ , M. Hittinger² , K. Knoth² , H. Gross² , S. Frentzel¹ , A. Kuczaj¹ , J. Hoeng¹ , T. Krebs³ , M. Peitsch^1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland
² PharmBioTec GmbH, Science Park 1, 66123 Saarbrücken, Germany
³ Vitrocell^® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany

The poster shows the use of the Vitrocell® Powder Chamber for dry powder testing by online monitoring of the powder mass deposition, by QCMs Nicotine quantification, particle number deposition and particle size distribution.

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DOI: 10.2217/nnm-2017-0358

Savvina Chortarea^1,2, Kleanthis Fytianos^1,3,4, Laura Rodriguez-Lorenzo¹, Alke Petri-Fink^1,5 & Barbara Rothen-Rutishauser^1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
²Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
³Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
⁴Department of Clinical Research, University of Bern, Bern, Switzerland
⁵Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

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DOI: 10.1089/aivt.2017.0042

David Thorne, Emma Bishop, Linsey Haswell, and Marianna Gaca
British American Tobacco Group R&D, Southampton, United Kingdom

This case study has demonstrated that dosimetry techniques using particulate markers can allow in vitro biological data generated on five independent aerosol exposure system studies, under contrasting experimental designs and with different cell types, to be directly compared.

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Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

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Marius Hittinger,¹ Sarah Barthold,² Lorenz Siebenbürger,¹ Kilian Zäh,³ Alexander Gress,¹ Sabrina Guenther,¹ Birgit Wiegand,¹ Carsten Boerger,¹ Markus Berger,⁴ Tobias Krebs,⁴ Henrik Groß,¹ and Claus-Michael Lehr¹,<sup>5

1</sup>PharmBioTec GmbH, Saarbrücken, Germany
²Saarland University, Department of Pharmacy, Saarbrücken, Germany
³Scienceneering UG, Blieskastel, Germany
⁴Vitrocell Systems GmbH, Waldkirch, Germany
⁵Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), HZI, Saarbrücken, Germany

The VITROCELL® Dry Powder Chamber is designed for aerosol deposition of different sized particles separated by their time of flight. For the development of this new instrument salbutamol sulphate was successfully exposed to human pulmonary epithelial cell culture by sedimentation and diffusion in vitro.

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