Vitrocell Systems GmbH - microbalance

4. Nov. 2019

Investigation of multiple whole smoke dosimetry techniques using a VITROCELL®VC10® smoke exposure system

https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keyser^a, Robert Leverette^a, Michael Hollings^b, Adam Seymour^b, Lesley Reeve^b, Wanda Fields^a
^a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
^b Covance Laboratories Ltd., North Yorkshire, UK

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

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Categories: Publications

Tags: tobacco smoke, VC 10 smoking robots, VITROCELL 6, microbalance, VITROCELL 12, photometer, air-liquid Interface

1. Nov. 2019

Electronic Cigarette Vapor With Nicotine Causes Airway Mucociliary Dysfunction Preferentially via TRPA1 Receptors

DOI: 10.1164/rccm.201811-2087OC

Samuel Chung ^{1 2}, Nathalie Baumlin ^{1 2}, John S Dennis ^{1 2}, Robert Moore², Sebastian F Salathe², Phillip L Whitney ², Juan Sabater ³, William M Abraham³, Michael D Kim ^{1 2}, Matthias Salathe ^{1 2}
¹Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Kansas.
²Division of Pulmonary, Critical Care and Sleep Medicine, University of Miami School of Medicine, Miami, Florida; and.
³Department of Research, Mount Sinai Medical Center, Miami Beach, Florida.

The transient receptor potential ankyrin 1 (TRPA1) is a molecular target for vape effects due to its expression in airway epithelia and its reported gating by nicotine, reactive oxidants, and flavors, especially cinnamaldehyde. To test whether nicotine had effects independent of other e-cig vapor constituents, the Vitrocell® CLOUD exposure system was utilized to nebulize fixed nicotine doses onto the apical surface of ALI cultures. A549 cell cultures were exposed to nicotine containing e-cig vapor, produced by the VC-1 smoke exposure robot, in the air-liquid interface.

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Categories: Publications

Tags: inhalation exposure, e-cigarettes, microbalance, VITROCELL Cloud, VC 1 smoking machine, air-liquid Interface

5. Apr. 2019

Assessing the respiratory toxicity of dihydroxyacetone using an in vitro human airway epithelial tissue model

https://doi.org/10.1016/j.tiv.2019.04.007
Yiying Wang^a, Qiangen Wu^b, Levan Muskhelishvili^c, Kelly Davis^c, Matthew Bryant^b, Xuefei Cao^a
^aDivision of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
^bDivision of Biochemistry Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
^c Toxicologic Pathology Associates, Jefferson, AR 72079, United States of America

Unintentional exposure of respiratory mucosal surfaces to DHA aerosols is highly possible during sunless tanning sessions, a possibility for which it is not approved. In this study, the potential respiratory toxicity of DHA using the ALI airway tissue model is evaluated. To mimic in vivo exposure conditions, a liquid aerosol generation and exposure system was used and exposed ALI cultures to a range of non-cytotoxic doses of DHA aerosols at the air-liquid interface.

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Categories: Publications

Tags: microbalance, VITROCELL Cloud, aerosol generator, air-liquid Interface

10. Mar. 2019

New Products at VITROCELL® User Group Meeting 2019

09 March 2019, Hilton Inner Harbour Hotel, Baltimore, USA

Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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20. Dec. 2018

Establishing the Vitrocell® Powder Chamber as a particle size-selective platform for in vitro dry powder testing

S. Steiner¹ , M. Hittinger² , K. Knoth² , H. Gross² , S. Frentzel¹ , A. Kuczaj¹ , J. Hoeng¹ , T. Krebs³ , M. Peitsch¹
¹ PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland
² PharmBioTec GmbH, Science Park 1, 66123 Saarbrücken, Germany
³ Vitrocell^® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany

The poster shows the use of the Vitrocell® Powder Chamber for dry powder testing by online monitoring of the powder mass deposition, by QCMs Nicotine quantification, particle number deposition and particle size distribution.

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Categories: Publications

Tags: microbalance, powder chamber

18. Dec. 2018

Development of a quantitative method for assessment of dose in in vitro evaluations using a VITROCELL® VC10® smoke exposure system

https://doi.org/10.1016/j.tiv.2018.12.010

Brian M. Keyser^a, Robert Leverette^a, Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a
^aRAI Services Company1, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
^b Covance Laboratories Ltd., North Yorkshire, UK

The aims of this study were to investigate dosimetry during whole smoke exposure in the VITROCELL® VC10® smoking robot using QCMs and to determine whether photometers could be used for concurrent assessment of dose during in vitro whole smoke exposures.

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Categories: Publications

Tags: tobacco smoke, VC 10 smoking robots, microbalance, photometer

6. Jun. 2018

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

DOI: 10.2217/nnm-2017-0358

Savvina Chortarea^1,2, Kleanthis Fytianos^1,3,4, Laura Rodriguez-Lorenzo¹, Alke Petri-Fink^1,5 & Barbara Rothen-Rutishauser¹
¹BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
²Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
³Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
⁴Department of Clinical Research, University of Bern, Bern, Switzerland
⁵Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, microbalance, liquid aerosol, VITROCELL Cloud, air-liquid Interface, airborne particles

26. May. 2018

Acute effects of multi-walled carbon nanotubes on primary bronchial epithelial cells from COPD patients

DOI: 10.1080/17435390.2018.1472310

Beyeler S^1,2, Chortarea S^3,4, Rothen-Rutishauser B³, Petri-Fink A³, Wick P⁴, Tschanz SA⁵, von Garnier C^1,2, Blank F^1,2.

¹ Department of BioMedical Research , University of Bern , Bern , Switzerland.
² Department of Pulmonary Medicine , University Hospital of Bern , Bern , Switzerland.
³ BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg , Switzerland.
⁴ Laboratory for Particles-Biology Interactions, Empa Swiss Federal Laboratories for Material Science and Technology , St. Gallen , Switzerland.
⁵Institute of Anatomy, University of Bern , Bern, Switzerland.

This paper shows the investigation of acute effects of occupational multi-walled carbon nanotubes exposure in primary epithelial lung cell cultures from potentially susceptible individuals with COPD.

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Categories: Publications

Tags: inhalation exposure, nanoparticles, microbalance, liquid aerosol, VITROCELL Cloud, air-liquid Interface, airborne particles

5. Apr. 2018

A Case Study for the Comparison of In Vitro Data Across Multiple Aerosol Exposure Studies with Extrapolation to Human Dose

DOI: 10.1089/aivt.2017.0042

David Thorne, Emma Bishop, Linsey Haswell, and Marianna Gaca
British American Tobacco Group R&D, Southampton, United Kingdom

This case study has demonstrated that dosimetry techniques using particulate markers can allow in vitro biological data generated on five independent aerosol exposure system studies, under contrasting experimental designs and with different cell types, to be directly compared.

Comments (0) Number of views (883)

Categories: Publications

Tags: inhalation exposure, tobacco smoke, e-cigarettes, VC 10 smoking robots, VITROCELL 6, microbalance, VITROCELL 12, VC 1 smoking machine, air-liquid Interface