Advanced in vitro exposure systems.

14. Jan. 2021

Toxic effects of gunshot fumes from different ammunitions for small arms on lung cells exposed at the air liquid interface

https://doi.org/10.1016/j.tiv.2021.105095


Espen Mariussen a,b, Lise Fjellsbø a,c, Tomas Roll Frømyr b, Ida Vaa Johnsen b, Tove Engen Karsrud b, Øyvind Albert Voie b
a Norwegian Institute for Air Research (NILU), PO Box 100, NO-2027 Kjeller, Norway
b Norwegian Defence Research Establishment (FFI), PO Box 25, NO-2027 Kjeller, Norway
c Norwegian University of Life Sciences (NMBU), PO Box 5003, NO-1432 Ås, Norway

 

Highlights
•Gunshot fumes are cytotoxic and induce DNA-damage in lung cells exposed at the air liquid interface.
•Shooting from small arms generate fumes with substantial amounts of nanosized materials.
•Toxicity of the gunshot fumes differed between different types of ammunition.

 

Read more

Comments (0) Number of views (99)

19. Nov. 2020

Sub‑ohm vaping increases the levels of carbonyls, is cytotoxic, and alters gene expression in human bronchial epithelial cells exposed at the air–liquid interface

https://doi.org/10.1186/s12931-020-01571-1


Alexandra Noël, Ekhtear Hossain, Zakia Perveen, Hasan Zaman and Arthur L. Penn
Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA

 

Human bronchial epithelial cells (H292) were exposed to either butter-flavored or cinnamon-flavored e-cig aerosols at the ALI in a Vitrocell exposure system connected to a third-generation e-cig device.

 

Read more

Comments (0) Number of views (192)

29. Sep. 2020

Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives

https://doi.org/10.1007/s11626-020-00517-7


Xuefei Cao1, Jayme P. Coyle2, Rui Xiong1, Yiying Wang1, Robert H. Heflich1, Baiping Ren1, William M. Gwinn3, Patrick Hayden4, Liying Rojanasakul2

1 Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Rd., AR Jefferson, USA
2 Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers forDisease Control and Prevention,Morgantown,WV, USA
3 Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Durham, NC, USA
4 BioSurfaces Inc., Ashland, MA, USA


One important element for validating any new assay for making regulatory decisions is determining its performance relative to an accepted standard. Conducting in vivo inhalation toxicity studies using whole-body or nose-only exposure systems is expensive and time-consuming and typically requires a large number of animals. The goal of using alternative methods, like human in vitro ALI airway cultures, ultimately is to replace inhalation toxicity testing in animals with in vitro approaches. Transition from animal- to human-based models is ultimately expected to lead to faster and better predictive toxicity assessments and therapeutic development at lower cost.  This study shows the development and validation of alternative in vitro methods for acute toxicity testing, including acute inhalation toxicity testing.
 

Read more

Comments (0) Number of views (234)

16. Sep. 2020

Quartz crystal microbalances (QCM) are suitable for real-time dosimetry in nanotoxicological studies using VITROCELL®Cloud cell exposure systems

https://doi.org/10.1186/s12989-020-00376-w


Yaobo Ding1,2 , Patrick Weindl1,2,3, Anke-Gabriele Lenz1,2, Paula Mayer1,2, Tobias Krebs3 and Otmar Schmid1,2
1Institute of Lung Biology and Disease, Helmholtz Zentrum München, 85764 Neuherberg, Germany
2Comprehensive Pneumology Center, Munich (CPC-M) - Member of the German Center for Lung Research (DZL), 81377 Munich, Germany
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany.

 

This study provides evidence that QCMs are suitable for real-time dosimetry in particle toxicology studies with cell cultures under air-liquid interface conditions. An experimental method for determination of LoD (lower limit of detection), accuracy and precision of QCMs using a fluorescent tracer (fluorescein salt) was presented and applied to the QCMs integrated in the VITROCELL® Cloud 6 and Cloud 12 aerosol-cell exposure systems.

 

Read more

Comments (0) Number of views (257)

24. Aug. 2020

Product News 08/2020

VITROCELL® Cloud Alpha 96

96-well Air/Liquid Interface (ALI) exposure. For reliable high throughput testing of cell cultures.

The VITROCELL® Cloud Alpha 96 is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines high throughput testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12- and 24-well). It’s functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the air/liquid interface have never been easier.

VITROCELL® Cloud Alpha 96

Read more

Comments (0) Number of views (772)

27. Jul. 2020

An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols

doi:10.3390/ijms21155335


Hana Barosova 1,2 , Bedia Begum Karakocak 1 , Dedy Septiadi 1 , Alke Petri-Fink 1,3, Vicki Stone 4 and Barbara Rothen-Rutishauser 1,
1 BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, 
2 Institute of Experimental Medicine of the Czech Academy of Sciences, 142 20 Prague, Czech Republic
3 Department of Chemistry, University of Fribourg, 1700 Fribourg, Switzerland
4 Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh EH14 4AS, UK


The in vitro co-culture model consisting of three human cell lines were exposed at the ALI using the VITROCELL® Cloud system, equiped with QCM allowing to measure and record the deposited dose online. The nebulizer was ideal for the aerosols to suffciently mix within the entire chamber, hence resulting in uniform droplet deposition. This study shows, that this model is not limited to testing potentially hazardous nanomaterials to human cell line co-culture models.

 

Read more

Comments (0) Number of views (195)

24. Feb. 2020

In vitro advances in whole aerosol approaches for e-cigarette testing

IIVS Workshop 3, Feb 24-26th 2020

Autor

David Thorne, British American Tobacco

 

Workshop series to identify, discuss and develop recommendations for the optimal generation and use of in vitro data for product regulation.

 

Read more

Comments (0) Number of views (271)

30. Nov. 2019

Dosimetric Analysis of Aerosol Generated by a Vitrocell® VC10® Smoking Robot – Investigations on Dose Dependency and Consistency of Application


Adam Seymour, Michael Hollings, Joanne Larner and Julie Clements;

Covance Laboratories Ltd, Harrogate, UK


QCMs were used to assess the deposition of cigarette aerosol from a Vitrocell® VC10® in Vitrocell® Ames, 6/4, 12/4 and 24/4 exposure modules. The power to resolve difference between 8 and 1 SLPM is statistically significant in all module types assessed. Uniformity within each module was confirmed (P >0.98) in all module types, at all airflows. Repeatability was assessed and found to be consistent (no significant difference) in all module types at airflows of 10, 8 and 4 SLPM, however, at 1 SLPM a significant difference was noted in 9 out of 12 experiments – likely an artifact of high deposition.
These results show that exposures utilizing the Vitrocell® VC10® and associated modules are robust; but indicate the necessity of dosimetry measures to aid in the quantification of delivered dose.
 

Read more

Comments (0) Number of views (133)

4. Nov. 2019

Investigation of multiple whole smoke dosimetry techniques using a VITROCELL®VC10® smoke exposure system

https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keysera, Robert Leverettea, Michael Hollingsb, Adam Seymourb, Lesley Reeveb, Wanda Fieldsa
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
b Covance Laboratories Ltd., North Yorkshire, UK

 

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

 

Read more

Comments (0) Number of views (1827)

1. Nov. 2019

Electronic Cigarette Vapor With Nicotine Causes Airway Mucociliary Dysfunction Preferentially via TRPA1 Receptors

DOI: 10.1164/rccm.201811-2087OC


Samuel Chung 1 2, Nathalie Baumlin 1 2, John S Dennis 1 2, Robert Moore 2, Sebastian F Salathe 2, Phillip L Whitney 2, Juan Sabater 3, William M Abraham 3, Michael D Kim 1 2, Matthias Salathe 1 2
1Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Kansas.
2Division of Pulmonary, Critical Care and Sleep Medicine, University of Miami School of Medicine, Miami, Florida; and.
3Department of Research, Mount Sinai Medical Center, Miami Beach, Florida.
 

The transient receptor potential ankyrin 1 (TRPA1) is a molecular target for vape effects due to its expression in airway epithelia and its reported gating by nicotine, reactive oxidants, and flavors, especially cinnamaldehyde. To test whether nicotine had effects independent of other e-cig vapor constituents, the Vitrocell® CLOUD exposure system was utilized to nebulize fixed nicotine doses onto the apical surface of ALI cultures. A549 cell cultures were exposed to nicotine containing e-cig vapor, produced by the VC-1 smoke exposure robot, in the air-liquid interface.

 

Read more

Comments (0) Number of views (207)
RSS
12345
Back to Top