Advanced in vitro exposure systems.

19. Jan. 2023

2023 User Group Meeting

Download the Preview folder and register as participant (customers only)

We wish to invite you to participate at our User Group Meeting 2023, an excellent opportunity to discuss our latest developments.

 

In the preview flyer you find an overview of the topics that will be discussed:

User Group Meeting 2023 - Preview  (PDF)

 

VITROCELL® User Group Meeting is on March 18, 2023.
It takes place at the Omni Nashville Hotel, Nashville, Tennessee, USA

If you want to be a part of it:

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18. Jan. 2023

Comparing the Toxicological Responses of Pulmonary Air–Liquid Interface Models upon Exposure to Differentially Treated Carbon Fibers

https://doi.org/10.3390/ijms24031927

Alexandra Friesen 1, Susanne Fritsch-Decker 2, Sonja Mülhopt 3 , Caroline Quarz 1, Jonathan Mahl 3, Werner Baumann 3, Manuela Hauser 3, Manuela Wexler 3, Christoph Schlager 4, Bastian Gutmann 4, Tobias Krebs 4, Ann-Kathrin Goßmann 5 , Frederik Weis 5 , Matthias Hufnagel 1, Dieter Stapf 3 , Andrea Hartwig 1, and Carsten Weiss 2,
1 Karlsruhe Institute of Technology (KIT), Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, 76131 Karlsruhe, Germany
2 Karlsruhe Institute of Technology (KIT), Institute of Biological and Chemical Systems, Biological Information Processing, 76344 Eggenstein-Leopoldshafen, Germany
3 Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, Germany
4 Vitrocell Systems GmbH, 79183 Waldkirch, Germany
5 Palas GmbH, 76229 Karlsruhe, Germany

 

In this study, we assessed the toxicological responses to mechanically (mCF) and thermo-mechanically treated PAN-based carbon fibers (tmCF), applied to BEAS-2B mono-, BEAS-2B/dTHP-1 co-, and BEAS-2B/dTHP-1/CCD-33Lu triple-cultures at an air–liquid interface. The results emphasize the need to further characterize the hazard of CF and the importance of determining exposure levels at workplaces as well as safety measures during the use of CF-containing materials. Moreover, the development and application of more complex in vitro models of the human lung help to establish new approach methodologies in the general field of particle and fiber toxicology and contribute to a reduction in in vivo investigations in the context of the 3R initiative.

 

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18. Nov. 2022

Rescue of Ciliary Function in Cell-based Primary Ciliary Dyskinesia Models using Nebulized, LNP-formulated mRNA

Rumpa B. Bhattacharjee1, Mirko Hennig1, Daniella Ishimaru1, David Liston1, Jackson K. Eby2, Christian C. Corona1, Ishita Agarwal1 Jade E. Casillas1, Shohan Molla1, Maninder S. Sidhu1, Julia Poniatowski1, Sierra R. Comini1, Ally Ashworth1, Xueliang Yu2, Peiyang Gao2, Harriet E. Lister1, Omid Mousa1, Hannah L. Golliher3, Patrick R. Sears3, Weining Yin3, Michael J. Torres2, Daniel J. Siegwart4, Vladimir G. Kharitonov1, Lawrence E. Ostrowski3, David J. Lockhart1, Brandon A. Wustman1
1 ReCode Therapeutics, Inc., Menlo Park, CA, 
2 ReCode Therapeutics, Inc., Dallas, TX,
3 Dept of Cell Biology and Physiology, UNC-Chapel Hill, NC, 4UT Southwestern Medical Center, Dallas, TX

 

Rescue of Ciliary Function in Cell-based Primary Ciliary Dyskinesia Models using Nebulized, LNP-formulated mRNA ReCode Therapeutics is developing an mRNA-based therapy for the treatment of PCD caused by mutations in DNAI1. The DNAI1 mRNA is sequence optimized and formulated in a proprietary SORT lipid nanoparticle (LNP). The LNP formulated mRNA is nebulized and delivered as an aerosol directly into the airway by using a VITROCELL® Cloud 12. Using knockdown human bronchial epithelial (hBE) cultures and knockout mouse tracheal cultures we show that ReCode’s LNP can restore ciliary activity.

 

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27. Oct. 2022

VITROCELL® sQCM 12: Data Interpretation

The Quartz Crystal Microbalance is a powerful tool for dosimetry in VITROCELL Exposure Systems. However, interpreting live-data from a sQCM can be misleading, as there is a lot going on in parallel during measurements.

VITROCELL Application Note

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13. Oct. 2022

Toxicological impact of organic ultrafine particles (UFPs) in human bronchial epithelial BEAS-2B cells at air-liquid interface

https://doi.org/10.1016/j.tiv.2021.105258

A. T. Juarez Facio1, J. Yon2, C. Corbière1, T. Rogez-Florent1, C. Castilla3, H. Lavanant3, M. Mignot3, C. Devouge-Boyer3, C. Logie1, L. Chevalier 4, J.-M. Vaugeois1, C. Monteil 1
1 Normandie Univ, UNIROUEN, UNICAEN ABTE, 76000 Rouen, France
2 Normandie Univ, UNIROUEN, INSA Rouen, CNRS, CORIA, 76000 Rouen, France.
3 Normandie Univ, INSA Rouen, UMR 6014 CNRS, COBRA, 76801, Saint Etienne Du Rouvray, France
4 Normandie Univ, UNIROUEN, INSA Rouen, CNRS, GPM-UMR6634, 76000 Rouen, France

 

Highlights

  • BEAS-2B cells were successfully exposed at ALI to primary UFPs emissions
  • PAHs-rich UFPs induced an acute cellular toxicity at low exposed dose
  • PAHs-rich UFPs activated oxidative stress and xenobiotic metabolism
  • This methodological approach offers new perspectives to evaluate different kinds of UFPs

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30. Sep. 2022

NAM-based prediction of point-of-contact toxicity in the lung: A case example with 1,3-dichloropropene

https://doi.org/10.1016/j.tox.2022.153340

Marjory Moreau, Jeff Fisher, Melvin E. Andersen, Asayah Barnwell, Sage Corzine, Aarati Ranade, Patrick D. McMullen, Scott D. Slattery
ScitoVation, LLC, 6 Davis Drive, Suite 146, Durham, NC 27709, USA

This case study has implemented a NAM-based chemical risk assessment approach that uses in vitro and in silico methods to account for pharmacodynamic and pharmacokinetic factors of in vivo toxicity. The experiments were performed with the immortalized cell lines A549 and BEAS-2B, of human alveolar and bronchial origin, respectively, to identify a dose range likely to produce a toxic effect in the differentiated cultures. A549 and BEAS-2B cells, grown at the ALI on Transwell permeable supports, were exposed to vapor for 4 h in a Vitrocell 12/12 exposure system, and viability was assessed 24 h after the initiation of exposure.

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30. Sep. 2022

Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro

https://doi.org/10.3390/nano12193431

Kirsty Meldrum, Joana A. Moura, Shareen H. Doak and Martin J. D. Clift
In Vitro Toxicology Group, Swansea University Medical School, Swansea University, Wales SA2 8PP, UK
 

The aim of this study was to develop further an established static in vitro model used to test ENP aerosol toxicology to incorporate fluid-flow dynamics. This was achieved by implementing the QuasiVivoTM system with a characterised A459_dTHP-1 coculture exposed in the Vitrocell Cloud12 to different particle samples at the ALI. 

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26. Sep. 2022

Product News 06-9/2022

VITROCELL® Cloud Alpha MOVE

For fully automated and repeated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts. Testing of up to 12 dose ranges in one experiment.

The VITROCELL® Cloud Alpha MOVE is our newest innovation and presents another great leap forward in fully automated exposure of cell cultures. It is the ideal device to obtain a dose-response relationship in one experiment.

VITROCELL® Cloud Alpha MOVE  (PDF)

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29. Jul. 2022

Advanced Respiratory Models for Hazard Assessment of Nanomaterials—Performance of Mono-, Co- and Tricultures

https://doi.org/10.3390/nano12152609

Laura Maria Azzurra Camassa 1, Elisabeth Elje 2,3, Espen Mariussen 2,4, Eleonora Marta Longhin 2, Maria Dusinska 2, Shan Zienolddiny-Narui 1, and Elise Rundén-Pran 2
1 National Institute of Occupational Health in Norway, 0033 Oslo, Norway
2 NILU—Norwegian Institute for Air Research, 2027 Kjeller, Norway
3 Institute of Basic Medical Sciences, Department of Molecular Medicine, University of Oslo, 0372 Oslo, Norway
4 Norwegian Institute of Public Health, FHI, 0456 Oslo, Norway
 

This study aimed at testing the robustness and sensitivity, characterizing, and optimizing an advanced respiratory model built on human alveolar epithelial A549 cells and evaluating its response to aerosol exposure of silver NM-300K. In more detail, we compared the responses of A549 cells cultured at the ALI in the VITROCELL® 6 Cloud system in monoculture, in coculture with EA.hy926 cells, and in triculture with EA.hy926 cells and dTHP-1, and performed an interlaboratory trial across two laboratories.

 

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21. Jun. 2022

Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

https://doi.org/10.1016/j.envint.2022.107366

Michal Pardoa, Svenja Offerbc, Elena Hartnerbc, Sebastiano Di Bucchianicob, Christoph Bisigb, Stefanie Bauerb, Jana Pantzkebc, Elias J. Zimmermannbc, XinCaobc, Stephanie Binderbc, Evelyn Kuhnb, Anja Huberb, Seongho Jeongbc, Uwe Käferbc, Eric Schneiderc,[...]

a Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, 234 Herzl Street, POB 26, ISR-7610001 Rehovot, Israel
b Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics (CMA), Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
c Joint Mass Spectrometry Center (JMSC) at Analytical Chemistry, Institute of Chemistry, University of Rostock, Dr.-Lorenz-Weg 2, D-18059 Rostock, Germany
d Department of Environmental and Biological Sciences, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland
e University of the Bundeswehr Munich, Institute for Chemistry and Environmental Engineering, Werner- Heisenberg-Weg 39, D-85577 Neubiberg, Germany
f Institute for Environmental Studies, Faculty of Science, Charles University, Albertov 6, CZE-12800 Prague, Czech Republic
g Department of Applied Physics, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland
h Institute of Energy and Climate Research, Troposphere (IEK-8), Forschungszentrum Jülich GmbH, Wilhelm-Johen-Str., D-52428 Jülich, Germany
i Department of Environmental Sciences, University of Basel, Klingelbergstr. 27, CH-4056 Basel, Switzerland
j Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Str. 10, D-13125 Berlin, Germany
k Institute of Computational Biology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
l The Mantoux Bioinformatics Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot 76100, Israel

 

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