Advanced in vitro exposure systems.

27. Jun. 2021

Impact of Nanocomposite Combustion Aerosols on A549 Cells and a 3D Airway Model

https://doi.org/10.3390/nano11071685

Matthias Hufnagel 1, Nadine May 2, Johanna Wall 1, Nadja Wingert 3, Manuel Garcia-Käufer 3, Ali Arif 3, Christof Hübner 4, Markus Berger 5, Sonja Mülhopt 2, Werner Baumann 2, Frederik Weis 6, Tobias Krebs 5, Wolfgang Becker 4, Richard Gminski 3, Dieter Stapf 2, and Andrea Hartwig 1,


1 Department of Food Chemistry and Toxicology, Institute of Applied Biosciences, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany;
2 Institute for Technical Chemistry, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany; 
3 Institute for Infection Prevention and Hospital Epidemiology, Medical Center—University of Freiburg, Faculty of Medicine, University of Freiburg, 79110 Freiburg, Germany;
4 Fraunhofer Institute of Chemical Technology, 76327 Pfinztal, Germany; 
5 Vitrocell® Systems GmbH, 79183 Waldkirch, Germany; 
6 Palas GmbH, 76229 Karlsruhe, Germany; 

 

This study was the first to investigate the toxicological effects of well characterized aerosols released during combustion of thermoplastic nanocomposites using an air–liquid interface exposure system. Even though studies on the toxicological potential of combustion-generated particulate matter as well as VOCs have been published, none of them was designed to investigate the effect of the native aerosol using appropriate realistic lung cell culture models. In the current study we investigated the combustion behavior of PE-based nanocomposites on a lab-scale burner. As nanoscaled fillers TiO2 NP, CuO NP, as well as CNT were chosen for this study, with TiO2 NP representing a commonly used insoluble and inert nanomaterial, CuO NP as a known in vitro cyto- as well as genotoxic nanomaterial, and CNT as a fiber-shaped nanomaterial.

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21. Jun. 2021

In vitro hazard characterization of simulated aircraft cabin bleed-air contamination in lung models using an air-liquid interface (ALI) exposure system

https://doi.org/10.1016/j.envint.2021.106718

Rui-Wen He a,b, Marc M.G. Houtzager c, W.P. Jongeneel a, Remco H.S. Westerink b, Flemming R. Cassee a,b

a National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, the Netherlands
b Institute for Risk Assessment Sciences (IRAS), Toxicology Division, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80177, 3508 TD Utrecht, the Netherlands
c The Netherlands Organisation for Applied Scientific Research, TNO, P.O. Box 80015, 3508 TA Utrecht, the Netherlands

 

This unique experimental “Mini-BACS + AES” setup is able to provide steady conditions to perform in vitro exposure under ALI conditions to aircraft engine oil and hydraulic fluid fumes, generated at respectively 350 ◦C and 200 ◦C. Exposure of the Calu-3 monoculture and Calu-3 + MDM co-culture lung cell models to high levels of aircraft engine oil and hydraulic fluid fumes under ALI conditions can reduce TEER and viabilities of the cells, induce cytotoxicity, and increase production of proinflammatory cytokines. Hydraulic fluid fumes are more toxic than engine oil fumes on the mass concentration of fume basis, which may be related to higher abundance of OPs and smaller particle size of hydraulic fluid fumes. The toxicological data clearly reflect the potential health risks during fume events in aircraft cabins.

 

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20. Apr. 2021

An In Vitro Air-Liquid Interface Inhalation Platform for Petroleum Substances and Constituents

DOI: 10.14573/altex.2010211 


Sandra Verstraelen1, An Jacobs1, Jo Van Laer1, Karen Hollanders1, Masha Van Deun2, Diane Bertels2, Rob Brabers1, Hilda Witters1, Sylvie Remy1,3, Lieve Geerts1, Lize Deferme4 and Evelien Frijns1
1VITO NV (Flemish Institute for Technological Research), Unit HEALTH, Mol, Belgium; 
2VITO NV, Unit Separation and Conversion Technology (SCT), Mol, Belgium; 
3University of Antwerp, Department of Biomedical Sciences, Antwerp, Belgium; 
4ExxonMobil Petroleum and Chemical BV, Machelen, Belgium
 

This pilot study exposed a frequently used in vitro model (A549 cells) at the ALI to assess inhalation toxicity of the single compound EB. Experimental conditions using the VITROCELL® 24/48 exposure system were optimized to achieve a (low) delivery efficiency that resulted in dose-dependent biological changes. The data demonstrate consistency in effect levels when comparing cell viability in the ALI experiments with known in vivo non-lethal effects in humans. It can be concluded that QIVIVE from in vitro air concentrations applied for testing cell viability to in vivo air concentrations may be a promising method for screening for acute inhalation toxicity. 

 

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19. Apr. 2021

Product News 04/2021

The new VITROCELL® Millicell Holder

How to use Millicell 24-well Standing Inserts in the VITROCELL® 12 Module Series

The revolutionary new holder system enables for the use of standing inserts in all modules of the VITROCELL® 12 Series for 12-well sized inserts. As an additional advantage, the media com­partment is sealed towards the aerosol exposure head to avoid contact of the test substance with the cell culture media.

VITROCELL® Millicell Holder – How to use Millicell 24-well Standing inserts in the VITROCELL® 12 Module Series

 

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3. Mar. 2021

Vitrocell® Leak Tester

Must-have device for aerosol research – easy and efficient leak detection

Regular leak tests are mandatory in aerosol research. A leak caused by a forgotten connection or defective o-ring may have a significant influence on the aerosol exposure process. We recommend to carry out a leak test prior to the experiment and as part of cleaning or service routine.

VITROCELL Application Note

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23. Feb. 2021

Product News 02/2021

The New VITROCELL® RH/T-Controller

Reliable digital Humidity- and Temperature Measurement

The VITROCELL® RH/T-Controller system was designed to measure temperature and relative humidity in numerous applications at high precision. It can be fitted to humidification systems, aerosol ducts and climatic chambers.

VITROCELL® RH/T-Controller – Reliable digital Humidity- and Temperature Measurement

 

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29. Jan. 2021

A Bioinspired in vitro Lung Model to Study Particokinetics of Nano-/Microparticles Under Cyclic Stretch and Air-Liquid Interface Conditions

DOI: 10.3389/fbioe.2021.616830 


Ali Doryab 1,2, Mehmet Berat Taskin 3, Philipp Stahlhut 3, Andreas Schröppel 1,2, Sezer Orak 1,2, Carola Voss 1,2, Arti Ahluwalia 4,5, Markus Rehberg 1,2, Anne Hilgendorff 1,2,6, Tobias Stöger 1,2, Jürgen Groll 3 and Otmar Schmid 1,2

1 Comprehensive Pneumology Center Munich, Member of the German Center for Lung Research, Munich, Germany,
2 Helmholtz Zentrum München—German Research Center for Environmental Health, Institute of Lung Biology and Disease, Munich, Germany, 
3 Department of Functional Materials in Medicine and Dentistry, Bavarian Polymer Institute, University of Würzburg, Würzburg, Germany, 
4 Research Center “E. Piaggio”, University of Pisa, Pisa, Italy, 
5 Department of Information Engineering, University of Pisa, Pisa, Italy, 
6 Center for Comprehensive Developmental Care (CDeCLMU), Dr. von Haunersches Children’s Hospital University, Hospital of the Ludwig-Maximilians University, Munich, Germany
 

We have recently introduced a novel porous and elastic membrane for in vitro cell-stretch models of the lung cultured under ALI conditions (Doryab et al., 2020). This innovative hybrid biphasic membrane, henceforth referred to as Biphasic Elastic Thin for Air-liquid culture conditions (BETA) membrane, was developed to optimize membrane characteristics for  the two phases of cell-stretch experiments under ALI conditions, namely the initial cell seeding, attachment and growth phase under submerged cell culture conditions  (phase  I)  followed  by an ALI acclimatization and cell-stretch phase at the ALI (phase II). This patented aerosol-cell exposure unit has recently been made  commercially  available  as  VITROCELL® Cloud  MAX.

 

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22. Jan. 2021

Product News 01/2021

The New VITROCELL® Cloud Alpha MAX

Smallest nebulization volumes and high deposition efficiency

The VITROCELL® Cloud Alpha MAX is designed for small nebulization volumes and very high deposition efficiency. This is important when only small quantities of material are available or when expensive test substances need to be tested.

VITROCELL® Cloud Alpha MAX – Ideal for small quantities of test substance

 

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15. Jan. 2021

New Video: VITROCELL Cloud Alpha – Product Family Overview

For virus research, testing of inhaled drugs, chemicals and nanoparticles at the Air/Liquid Interface (ALI). The systems are suitable for nebulized solutions and suspensions.

The VITROCELL® Cloud Alpha series is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines reliable exposure of cell cultures from the respiratory tract with ease of use. The series comprises the Cloud Alpha 6, Cloud Alpha 12, Cloud Alpha 96 and Cloud Alpha MAX – they represent an optimal solution for everyday experiments at the Air/Liquid Interface using 6-well, 12-well, 24-well or 96-sized inserts.

This video showcases available product configurations.

 

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29. Dec. 2020

Air–Liquid Interface Exposure of Lung Epithelial Cells to Low Doses of Nanoparticles to Assess Pulmonary Adverse Effects

https://doi.org/10.3390/nano11010065

 

Silvia Diabaté 1, Lucie Armand 2, Sivakumar Murugadoss 1 , Marco Dilger 1 , Susanne Fritsch-Decker 1, Christoph Schlager 3, David Béal 2, Marie-Edith Arnal 2, Mathilde Biola-Clier 2, Selina Ambrose 4, Sonja Mülhopt 3, Hanns-Rudolf Paur 3, Iseult Lynch 5 , Eugenia Valsami-Jones 5 , Marie Carriere 2, and Carsten Weiss 1

1 Karlsruhe Institute of Technology, Institute of Biological and Chemical Systems–Biological Information Processing, 76344 Eggenstein-Leopoldshafen, Germany;
2 CEA, CNRS, IRIG, SyMMES, University Grenoble Alpes, 38054 Grenoble, France;
3 Karlsruhe Institute of Technology, Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, Germany; 
4 Promethean Particles Ltd., Nottingham NG7 3EF, UK;
5 School of Geography Earth & Environmental Sciences (GEES), University of Birmingham (UoB), Edgbaston, Birmingham B15 2TT, UK;

 

KIT, together with VITROCELL SYSTEMS, set up a first Automated Exposure Station, which has been used for the assessment of nanoscale particle emissions from combustion sources such as ship diesel and wood burners. The system was further developed and offers a compact solution for toxicity testing of nanoparticle (NP) aerosols including sample conditioning, reproducible deposition, integrated dose determination by a quartz crystal microbalance (QCM), flow control, automated processes and data acquisition. The device was also tested with partner laboratories with the aim of potentially standardizing and achieving regulatory acceptance of the method.

 

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