Autoren:
Hillemann, L.; Stintz, M.; Streibel, T.; Zimmermann, R.; Öder, S.; Kasurinen, S.; di Bucchianico, S.; Kanashova, T.; Dittmar, G.; Konzack, D.; Große, S.; Rudolph, A.; Berger, M.; Krebs, T.; Saraji-Bozorgzad, M. R.; Walte, A.

Die Wirkung von Emissionen aus dem Trennen von Carbonbeton werden in einer Kombination aus Trennstaubgenerator, Verdünnungssystem und Expositionssystem an Zellkulturen (A549 und THP-1) gegenüber den entstehenden Stäuben bestimmt (Zytotoxizität).

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https://doi.org/10.3390/pharmaceutics10020056 

Julia Metz ^1,2, Katharina Knoth ¹, Henrik Groß ¹, Claus-Michael Lehr ^1,2,3, Carolin Stäbler ⁴, Udo Bock ⁵, and Marius Hittinger ^1
1Department of Drug Delivery, PharmBioTec GmbH, 66123 Saarbrücken, Germany
²Department of Biopharmaceutics and Pharmaceutical Technology, Department of Pharmacy, Saarland University, 66123 Saarbrücken, Germany
³Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Center for Infection Research (HZI), 66123 Saarbrücken, Germany
⁴Department of Scientific Affairs Consumer Health, Bayer Vital GmbH, 51368 Leverkusen, Germany
⁵Bock Project Management, 54456 Tawern, Germany

In this study two innovative in vitro techniques were combined: The Vitrocell® Powder Chamber and the human cell based MucilAir™ system. Their combined use allows the investigation of the effect of aerosolized pollen and appropriate drugs on a human mucosal tissue, a similar scenario finding in humans. The release of pro-inflammatory cytokines (IL-6, IL-8, and TNF-α) was quantified as an initial cellular response to pollen contact and to confirm the protective effect of drugs.

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https://doi.org/10.1016/j.toxrep.2018.04.003

Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a,
^a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA2
^b Covance Laboratories Ltd., North Yorkshire, UK

The article shows the successfull qualification of the Vitrocell VC10 smoking robot. The robot was used to  expose bacterial cells to cigarette whole smoke generated from 3R4F reference cigarettes and Eclipse cigarettes. A comparison was made between whole smoke from a tobacco heating and a combustible cigarette.

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DOI: 10.1089/aivt.2018.0001
Holger Behrsing,¹ Mario Aragon,¹ Jason Adamson,² Devin Sheehan,¹ Marianna Gaca,² Rodger Curren,¹ and Erin Hill^1
1Respiratory Toxicology, Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.
²R&D, British American Tobacco, Southampton, United Kingdom.

The study shows an assessment of VC1 aerosol generation that was conducted by measuring puff-by-puff in vitro nicotine concentration of 3R4F cigarettes (ISO and HCI regimes), e-cigarette (CRM81 puffing profile), and THP (HCIm regime). The aerosols were applied to in vitro or ex vivo cellular and tissue systems that model the respiratory tract, such as reconstructed human airways (RHuA), which are grown at the air–liquid interface (ALI). Additionally a comparison between the Vitrocell VC1 (U.S. Laboratory) and the VC10 (UK Laboratory) Smoking Robot was done.

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Dr. Monique Manche¹, Dr. Fabrice Nesslany², Tobias Krebs^3
1Laboratoires Anios, 59260 Lille-Hellemmes, France
²Laboratoire de toxicologie génétique, Institut Pasteur de Lille, 59000 Lille, France
³VITROCELL Systems GmbH, 79183 Waldkirch, Germany

Genotoxicity tests (in vitro micronucleus test and comet assay) have been conducted on p53 competent pulmonary cells, with alcohol administration as vapours to obtain data under conditions more representative of actual exposure conditions via the pulmonary route.

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