https://doi.org/10.3390/ijms24031927

Alexandra Friesen ¹, Susanne Fritsch-Decker ², Sonja Mülhopt ³ , Caroline Quarz ¹, Jonathan Mahl ³, Werner Baumann ³, Manuela Hauser ³, Manuela Wexler ³, Christoph Schlager ⁴, Bastian Gutmann ⁴, Tobias Krebs ⁴, Ann-Kathrin Goßmann ⁵ , Frederik Weis ⁵ , Matthias Hufnagel ¹, Dieter Stapf ³ , Andrea Hartwig ¹, and Carsten Weiss ²,
¹ Karlsruhe Institute of Technology (KIT), Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, 76131 Karlsruhe, Germany
² Karlsruhe Institute of Technology (KIT), Institute of Biological and Chemical Systems, Biological Information Processing, 76344 Eggenstein-Leopoldshafen, Germany
³ Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, 76344 Eggenstein-Leopoldshafen, Germany
⁴ Vitrocell Systems GmbH, 79183 Waldkirch, Germany
⁵ Palas GmbH, 76229 Karlsruhe, Germany

In this study, we assessed the toxicological responses to mechanically (mCF) and thermo-mechanically treated PAN-based carbon fibers (tmCF), applied to BEAS-2B mono-, BEAS-2B/dTHP-1 co-, and BEAS-2B/dTHP-1/CCD-33Lu triple-cultures at an air–liquid interface. The results emphasize the need to further characterize the hazard of CF and the importance of determining exposure levels at workplaces as well as safety measures during the use of CF-containing materials. Moreover, the development and application of more complex in vitro models of the human lung help to establish new approach methodologies in the general field of particle and fiber toxicology and contribute to a reduction in in vivo investigations in the context of the 3R initiative.

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The Quartz Crystal Microbalance is a powerful tool for dosimetry in VITROCELL Exposure Systems. However, interpreting live-data from a sQCM can be misleading, as there is a lot going on in parallel during measurements.

VITROCELL Application Note

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https://doi.org/10.1016/j.tox.2022.153340

Marjory Moreau, Jeff Fisher, Melvin E. Andersen, Asayah Barnwell, Sage Corzine, Aarati Ranade, Patrick D. McMullen, Scott D. Slattery
ScitoVation, LLC, 6 Davis Drive, Suite 146, Durham, NC 27709, USA

This case study has implemented a NAM-based chemical risk assessment approach that uses in vitro and in silico methods to account for pharmacodynamic and pharmacokinetic factors of in vivo toxicity. The experiments were performed with the immortalized cell lines A549 and BEAS-2B, of human alveolar and bronchial origin, respectively, to identify a dose range likely to produce a toxic effect in the differentiated cultures. A549 and BEAS-2B cells, grown at the ALI on Transwell permeable supports, were exposed to vapor for 4 h in a Vitrocell 12/12 exposure system, and viability was assessed 24 h after the initiation of exposure.

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For fully automated and repeated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts. Testing of up to 12 dose ranges in one experiment.

The VITROCELL® Cloud Alpha MOVE is our newest innovation and presents another great leap forward in fully automated exposure of cell cultures. It is the ideal device to obtain a dose-response relationship in one experiment.

VITROCELL® Cloud Alpha MOVE  (PDF)

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https://doi.org/10.1016/j.envint.2022.107366

Michal Pardo^a, Svenja Offer^bc, Elena Hartner^bc, Sebastiano Di Bucchianico^b, Christoph Bisig^b, Stefanie Bauer^b, Jana Pantzke^bc, Elias J. Zimmermann^bc, XinCao^bc, Stephanie Binder^bc, Evelyn Kuhn^b, Anja Huber^b, Seongho Jeong^bc, Uwe Käfer^bc, Eric Schneider^c,[...]

^a Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, 234 Herzl Street, POB 26, ISR-7610001 Rehovot, Israel
^b Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics (CMA), Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
^c Joint Mass Spectrometry Center (JMSC) at Analytical Chemistry, Institute of Chemistry, University of Rostock, Dr.-Lorenz-Weg 2, D-18059 Rostock, Germany
^d Department of Environmental and Biological Sciences, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland
^e University of the Bundeswehr Munich, Institute for Chemistry and Environmental Engineering, Werner- Heisenberg-Weg 39, D-85577 Neubiberg, Germany
^f Institute for Environmental Studies, Faculty of Science, Charles University, Albertov 6, CZE-12800 Prague, Czech Republic
^g Department of Applied Physics, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland
^h Institute of Energy and Climate Research, Troposphere (IEK-8), Forschungszentrum Jülich GmbH, Wilhelm-Johen-Str., D-52428 Jülich, Germany
ⁱ Department of Environmental Sciences, University of Basel, Klingelbergstr. 27, CH-4056 Basel, Switzerland
^j Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Str. 10, D-13125 Berlin, Germany
^k Institute of Computational Biology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
^l The Mantoux Bioinformatics Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot 76100, Israel

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