Advanced in vitro exposure systems.

21. Oct. 2020

New Video: VITROCELL Cloud α – for Aerosol Research and Cell Exposure at the Air/Liquid Interface (ALI)

Cell exposure system for virus research, testing of inhaled drugs, chemicals and nanoparticles at the Air/Liquid Interface (ALI).

The VITROCELL® Cloud Alpha series is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines reliable exposure of cell cultures from the respiratory tract with ease of use. This series is our new take on the well-known and frequently published VITROCELL® Cloud systems (6-, 12- and 24-well). Its functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the air/liquid interface have never been easier. The Cloud system is suitable for nebulization of solutions and suspensions. Possible fields of application are screening of inhaled drugs, virus research toxicity testing of inhaled substances such as chemicals or nanoparticles.

In response to the scientific need to expose in physiologically relevant conditions, the VITROCELL® Cloud Alpha exposure devices have been specifically designed to enable direct exposure of mammalian cells or tissue at the Air/Liquid Interface. Here the cell cultures are not covered with media as opposed to submerged conditions which cause an undesired inter­action of the formerly airborne substances with the culture media.

The video explains the procedures of the experiment.

 

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28. Sep. 2020

NanoCELL - Comprehensive characterization and human toxicological assessment of cellulose nanocrystals along their life cycle for reliable risk assessment

Yvonne Kohl1, Roland Drexel2, Christine Herrmann3, Stephan Dähnhardt-Pfeiffer4, Siegfried Fürtauer5, Michelle Hesler1, Christoph Metzger3, Marielle Fink6, Dominik Selzer7, Thorsten Lehr7, Tobias Krebs6, Sven van Lengen8, Sylvia Wagner1, Hagen von Briesen1, Felix Grimm9, Petra Weißhaupt10, Heiko Briesen3, Florian Meier2


1Fraunhofer-Institut für Biomedizinische Technik IBMT, Sulzbach, Germany; 
2Postnova Analytics GmbH, Landsberg, Germany, 
3Technische Universität München, Lehrstuhl für Systemverfahrenstechnik, Freising, Germany 
4Microscopy Services Dähnhardt GmbH, Flintbek, Germany
5Fraunhofer-Institut für Verfahrenstechnik und Verpackung IVV, Freising, Germany
6VITROCELL Systems GmbH, Waldkirch, Germany
7Universität des Saarlands, Klinische Pharmazie, Saarbrücken, Germany 
8GRÜNPERGA Papier GmbH, Grünhainichen, Germany 
9INFIANA Germany GmbH & Co. KG, Forchheim, Germany
10Umweltbundesamt, Dessau-Roßlau, Germany

 

This poster shows the hazard studies on the effect of oral and pulmonary CNC uptake. Therefore a miniaturized cloud exposure system has been developed to create an in vitro model simulating the lung and the GI tract.

 

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18. Sep. 2020

VITROCELL® Shisha Testing System for Cell Cultures

For in vitro testing of water pipe tobacco at the air / liquid interface

New designs of electronic cigarettes such as ENDS (Electronic Nicotine Delivery Systems) products or HTP (Heated Tobacco Products) lead to a large variety of different shapes which make the insertion into conventional holders with labyrinth seals impossible. VITROCELL® has developed a new holder system which is flexible to adjust to different shapes.

VITROCELL Application Note

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24. Aug. 2020

Product News 08/2020

VITROCELL® Cloud Alpha 96

96-well Air/Liquid Interface (ALI) exposure. For reliable high throughput testing of cell cultures.

The VITROCELL® Cloud Alpha 96 is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines high throughput testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12- and 24-well). It’s functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the air/liquid interface have never been easier.

VITROCELL® Cloud Alpha 96

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27. Jul. 2020

Vitrocell® holder systems for e-cigarettes

Secure and tight connection of any device to the smoking machine.

New designs of electronic cigarettes such as ENDS (Electronic Nicotine Delivery Systems) products or HTP (Heated Tobacco Products) lead to a large variety of different shapes which make the insertion into conventional holders with labyrinth seals impossible. VITROCELL® has developed a new holder system which is flexible to adjust to different shapes.

VITROCELL Application Note

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25. Jun. 2020

Novel TEM Grid holders for dose determination of airborne particles during in vitro Exposure at the air / liquid interface

The assessment of toxicological effects of airborne particles to the human organism is of major importance in disease research. The presence of nano and ultrafine particles can be found both in indoor and outdoor atmospheres which include workplaces and living areas. Over the last years, the degree of contribution to diseases of such particles is analyzed ever more frequent by the use of in vitro methods. The required equipment for these studies consisting of aerosol generation and exposure systems are nowadays developed more and more to proven levels.

VITROCELL Application Note.

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13. May. 2020

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

DOI:10.3791/61210 


Hedwig M. Braakhuis1, Ruiwen He1,2, Rob J. Vandebriel1, Eric R. Gremmer1, Edwin Zwart1, Jolanda P. Vermeulen1, Paul Fokkens1, John Boere1, Ilse Gosens1, Flemming R. Cassee1,2 
1National Institute for Public Health and the Environment (RIVM) 
2Institute for Risk Assessment Sciences (IRAS) 


This article provides a method for culturing and exposing the human bronchial epithelial cell line Calu-37 at the Air-liquid Interface that mimics realistic, repeated inhalation exposure conditions that can be used for toxicity testing. By applying a continuous airflow using the Automated Exposure System, the cell model can be exposed to a low concentration of particles over a longer time period, reflecting realistic exposure conditions. Characteristics of both the cell model and of  the exposure system are essential for achieving a realistic inhalation exposure model that can be used for repeated exposures. 

 

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27. Mar. 2020

Reliable in vitro Exposure Systems for Screening of Antiviral Lead Compounds against Coronavirus (COVID-19 / SARS-CoV-2)

VITROCELL Application Note for Highly efficient and realistic application of aerosolized drugs to ­human cells of the respiratory tract under physiologic conditions

In inhalation therapy, drugs are deposited as aerosols on cells of the respiratory tract from the nasal or lung region. Physiologically realistic in vitro cell culture models of the pulmonary epithelium and the air-blood barrier as well as from the nasal region are commercially available. Recently, these models have been refined to mimic  SARS-CoV-2 infections.

VITROCELL Application Note.

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26. Mar. 2020

Screening antiviraler pharmazeutischer Leitwirkstoffe gegen das Coronavirus (COVID-19 / SARS-CoV-2)

VITROCELL in vitro Expositionssysteme für hocheffiziente und realistische Applikation aerosolisierter Medikamente auf menschliche Zellen des Atemtraktes unter physiologischen Bedingungen.

In der Inhalationstherapie werden pharmazeutische Präparate als Aerosole auf Zellen des Atemtraktes im Nasen- oder Lungenbereich appliziert. Zur präklinischen Entwicklung neuer Arzneimittel werden dafür oft kommerziell erhältliche, physiologisch relevante in vitro Zellkulturmodelle des Nasen-, Rachen- oder Lungenepithels verwendet. In jüngster Zeit wurden diese Modelle verfeinert, um SARS-CoV-2 Infektionen nachzuahmen.

VITROCELL Anwendungshinweis.

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24. Mar. 2020

Comparison of Vapor and Liquid Phase Acrolein Exposures to Air Liquid Interface (ALI) Cell Cultures

David H. Brandwein, F. Adam Bettmann, Michael P. DeLorme, Alan T. Eveland, Lawrence M. Milchak 
3M Corporate Toxicology and Environmental Science, St. Paul, MN
 

The STL is working to develop an in vitro screening ALI model to assess the acute respiratory irritation potential for new chemicals. These experiments examined multiple aspects of the model, including different cell culture systems (A549 and EpiAirway), different exposure methods (dynamic vapor and liquid phase), and different post exposure periods, all using acrolein as a model respiratory irritant. The goal was to better understand the critical parameters of the cell systems and exposure methods to enable the development of a consistent screening model, while gaining clarity of the dosimetry. 

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