Advanced in vitro exposure systems.

7. Sep. 2019

Application of a multi‑layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e‑liquid and its corresponding aerosol using an e‑cigarette device

https://doi.org/10.1007/s00204-019-02565-9


Anita R. Iskandar, Filippo Zanetti, Diego Marescotti, Bjorn Titz, Alain Sewer, Athanasios Kondylis, Patrice Leroy, Vincenzo Belcastro, Laura Ortega Torres, Stefano Acali, Shoaib Majeed, Sandro Steiner, Keyur Trivedi, Emmanuel Guedj, Celine Merg, Thomas Schneider, Stefan Frentzel, Florian Martin, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng


Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

Previous experimental setups shows the effects of e-liquids on cell viability (first layer), followed by investigating the potential mechanisms of toxicity elicited by e-liquids (second layer) and finally assessing the impacts of aerosols (third layer). In this present work shows how the three-layer framework is leveraged to evaluate the potential toxicity and biological effects of the MESH Classic Tobacco and Base e-liquids/aerosols compared with those of 3R4F CS.

 

Read more

Comments (0) Number of views (696)

11. Aug. 2019

Exposure to aerosols from electronic cigarettes using the MESH™ technology has a reduced biological impact on bronchial epithelial cell cultures compared with exposure to cigarette smoke

Gordon Research Conference, Integration of Emerging Technologies in Mechanistic and Translational Toxicology,Andover, August 11–16, 2019

Albert Giralt, Florian Martin, Anita R. Iskandar, Alain Sewer, Laura Ortega Torres, AthanasiosKondylis, Patrice Leroy, Celine Merg, ShoaibMajeed, Emmanuel Guedj, Thomas Schneider, KeyurTrivedi, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng


PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud5, CH-2000 Neuchâtel, Switzerland
 

In contrast to 3R4F CS exposure, exposure to IQOS MESH™ Classic Tobacco aerosols did not cause tissue damage or have an impact on ciliary beating functionality in bronchial epithelial cell cultures despite resulting in greater concentrations of deposited nicotine. Cultures exposed to IQOS MESH™ Classic Tobacco aerosols showed fewer changes in proteins involved in xenobiotic metabolism than those exposed to CS.

 

Read more

Comments (0) Number of views (648)

12. Jun. 2019

Molecular Signature of Asthma-Enhanced Sensitivity to CuO Nanoparticle Aerosols from 3D Cell Model

DOI: 10.1021/acsnano.9b01823

Ingeborg Kooter1, Marit Ilves 2, Mariska Gröllers-Mulderij 1, Evert Duistermaat 3, Peter C. Tromp 1, Frieke Kuper 1, Pia Kinaret 4,5, Kai Savolainen 6, Dario Greco 4,5, Piia Karisola 2, Joseph Ndika 2, and Harri Alenius 2,7

1The Netherlands Organization for Applied Scientific Research, TNO, P.O. Box 80015, Utrecht 3584 CB, The Netherlands
2Human Microbiome Research, Faculty of Medicine, University of Helsinki, P.O. Box 21, Helsinki 00290, Finland
3Triskelion B.V., P.O. Box 844, Zeist 3704 HE, The Netherlands
4Faculty of Medicine and Life Sciences, University of Tampere, Tampere FI-33014, Finland
5Institute of Biotechnology, University of Helsinki, P.O. Box 56, Helsinki 00014, Finland
6Finnish Institute of Occupational Health, P.O. Box 40, Helsinki 00014, Finland
7Institute of Environmental Medicine, Karolinska Institutet, P.O. Box 210, Stockholm SE-17176, Sweden

3D human bronchial epithelial cells were cultured at the air−liquid interface that mimics relevant inhalatory exposure were exposed to aerosols of pristine (nCuO) and carboxylated (nCuOCOOH) copper oxide nanoparticles. This paper shows that the existence of asthma enhances sensitivity of the airways to nanoparticle aerosols, possibly as a combined result of a hyperactive airway and inefficient mucociliary clearance mechanisms in asthmatics. The test results are shown in cell viabilty (LDH), Inflammation (IL6, IL8, MCP1) and Transcroptomics.

Read more

Comments (0) Number of views (2133)

10. Mar. 2019

New Products at VITROCELL® User Group Meeting 2019

09 March 2019, Hilton Inner Harbour Hotel, Baltimore, USA

Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

Read more

Comments (0) Number of views (96)

10. Mar. 2019

Exposure to an aerosol from a novel electronic cigarette using the MESH™ technology elicited reduced biological impacts than exposure to cigarette smoke on buccal and small airway epithelial cultures

Society of Toxicology, Annual Meeting 2019, Baltimore, MD, USA | 10-14 March

Anita R. Iskandar, Filippo Zanetti, Athanasios Kondylis, Florian Martin, Alain Sewer, Laura Ortega Torres, Shoaib Majeed, Sandro Steiner, Emmanuel Guedj, Celine Merg, Thomas Schneider, Keyur Trivedi, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng


PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

 

The impacts of an acute exposure to cigarette smoke (CS) and to aerosol from a novel electronic cigarette (EC) device using MESH™ technology were assessed using human organotypic buccal epithelial cultures and small airway epithelial cultures. A paired design was implemented: in parallel to the exposure to CS or EC aerosol, cultures were also exposed to air in the same exposure module. Tissue damage was not seen in cultures exposed to the IQOS MESH™ Classic Tobacco aerosol despite resulting in greater concentrations of deposited nicotine. In buccal cultures, CS and IQOS MESH™ Classic Tobacco aerosol elicited different infammatory response.

 

Read more

Comments (0) Number of views (37)

5. Mar. 2019

A lower impact of an acute exposure to electronic cigarette aerosols than to cigarette smoke in human organotypic buccal and small airway cultures was demonstrated using systems toxicology assessment

DOI:10.1007/s11739-019-02055-x
Filippo Zanetti1, Athanasios Kondylis1, Florian Martin1, Patrice Leroy1, Shoaib Majeed1, Sandro Steiner1, Yang Xiang1, Laura Ortega Torres1, Keyur Trivedi1, Emmanuel Guedj1, Celine Merg1, Stefan Frentzel1, Nikolai V. Ivanov1, Utkarsh Doshi2, Kyeonghee Monica Lee2, Willie J. McKinneyJr2, Manuel C. Peitsch1, Julia Hoeng1

1 Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
2 Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

 

Read more

Comments (0) Number of views (214)

6. Jun. 2018

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

DOI: 10.2217/nnm-2017-0358

Savvina Chortarea1,2, Kleanthis Fytianos1,3,4, Laura Rodriguez-Lorenzo1, Alke Petri-Fink1,5 & Barbara Rothen-Rutishauser1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
2Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
3Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
4Department of Clinical Research, University of Bern, Bern, Switzerland
5Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

 

Read more

Comments (0) Number of views (452)

1. Jun. 2018

Charakterisierung von Partikelemissionen aus dem Trennschleifprozess von kohlefaserverstärktem Beton (Carbonbeton)

Autoren:
Hillemann, L.; Stintz, M.; Streibel, T.; Zimmermann, R.; Öder, S.; Kasurinen, S.; di Bucchianico, S.; Kanashova, T.; Dittmar, G.; Konzack, D.; Große, S.; Rudolph, A.; Berger, M.; Krebs, T.; Saraji-Bozorgzad, M. R.; Walte, A.

Die Wirkung von Emissionen aus dem Trennen von Carbonbeton werden in einer Kombination aus Trennstaubgenerator, Verdünnungssystem und Expositionssystem an Zellkulturen (A549 und THP-1) gegenüber den entstehenden Stäuben bestimmt (Zytotoxizität).

 

Read more

Comments (0) Number of views (547)

26. May. 2018

Acute effects of multi-walled carbon nanotubes on primary bronchial epithelial cells from COPD patients

DOI: 10.1080/17435390.2018.1472310

Beyeler S1,2, Chortarea S3,4, Rothen-Rutishauser B3, Petri-Fink A3, Wick P4, Tschanz SA5, von Garnier C1,2, Blank F1,2.

1 Department of BioMedical Research , University of Bern , Bern , Switzerland.
2 Department of Pulmonary Medicine , University Hospital of Bern , Bern , Switzerland.
3 BioNanomaterials, Adolphe Merkle Institute, University of Fribourg , Fribourg , Switzerland.
4 Laboratory for Particles-Biology Interactions, Empa Swiss Federal Laboratories for Material Science and Technology , St. Gallen , Switzerland.
5 Institute of Anatomy, University of Bern , Bern, Switzerland.

This paper shows the investigation of acute effects of occupational multi-walled carbon nanotubes exposure in primary epithelial lung cell cultures from potentially susceptible individuals with COPD.

Read more

Comments (0) Number of views (506)

10. May. 2018

Combining MucilAir™ and Vitrocell® Powder Chamber for the In Vitro Evaluation of Nasal Ointments in the Context of Aerosolized Pollen

https://doi.org/10.3390/pharmaceutics10020056 

Julia Metz 1,2, Katharina Knoth 1, Henrik Groß 1, Claus-Michael Lehr 1,2,3, Carolin Stäbler 4, Udo Bock 5, and Marius Hittinger 1
1Department of Drug Delivery, PharmBioTec GmbH, 66123 Saarbrücken, Germany
2Department of Biopharmaceutics and Pharmaceutical Technology, Department of Pharmacy, Saarland University, 66123 Saarbrücken, Germany
3Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Center for Infection Research (HZI), 66123 Saarbrücken, Germany
4Department of Scientific Affairs Consumer Health, Bayer Vital GmbH, 51368 Leverkusen, Germany
5Bock Project Management, 54456 Tawern, Germany

In this study two innovative in vitro techniques were combined: The Vitrocell® Powder Chamber and the human cell based MucilAir™ system. Their combined use allows the investigation of the effect of aerosolized pollen and appropriate drugs on a human mucosal tissue, a similar scenario finding in humans. The release of pro-inflammatory cytokines (IL-6, IL-8, and TNF-α) was quantified as an initial cellular response to pollen contact and to confirm the protective effect of drugs.

 

Read more

Comments (0) Number of views (574)
RSS
12345678910Last
Back to Top