Advanced in vitro exposure systems.

11. Mar. 2018

A setup to test toxicity of alcohol inhalation from consumer products

User Group Meeting 2018

Dr. Monique Manche1, Dr. Fabrice Nesslany2, Tobias Krebs3
1Laboratoires Anios, 59260 Lille-Hellemmes, France
2Laboratoire de toxicologie génétique, Institut Pasteur de Lille, 59000 Lille, France
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

Genotoxicity tests (in vitro micronucleus test and comet assay) have been conducted on p53 competent pulmonary cells, with alcohol administration as vapours to obtain data under conditions more representative of actual exposure conditions via the pulmonary route.

 

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11. Mar. 2018

Update on FTIR integration

User Group Meeting 2018

Dr. Martin Kieninger1, Tobias Krebs2

1ANSYCO GmbH, Gasmet Group, 76131 Karlsruhe, Germany

2VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

The poster shows a reliable, easy to use online analysis tool with fast response and a wide range of components due to Calcmet library. Ideal for quality control and dose monitoring.


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11. Mar. 2018

VITROCELL® 6/48: Changeover set for 24-well sized inserts

User Group Meeting 2018

Tobias Krebs

VITROCELL Systems GmbH, 79183 Waldkirch, Germany 

 

The poster shows the VITROCEL® 48 exposure system with a 6- and a 24-well setup.

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11. Mar. 2018

New Products at VITROCELL® User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2018.

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20. Aug. 2017

Development of a 3D co-culture system to assess the respiratory sensitizing potential of chemicals

10th Congress on Alternatives and Animal Use in the Life Sciences August 20–24, 2017. Seattle, Washington, USA.

A. Chary 1,2, T. Serchi1, J. Hennen2, B. Bloemeke2, J. Ezendam3, S. Cambier1, E. Moschini1, A.C. Gutleb1
1Department of Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg,
2Department of Environmental Toxicology, University of Trier, Trier, Germany,
3RIVM Centre for Environmental Protection (GZB), National Institute for Public Health and the Environment (RIVM), Utrecht, The Netherlands.

The aim of this study is to adapt the 3D-co-culture system previously developed by LIST (Klein et al.1,2) to allow the in vitro study of respiratory sensitization processes and to
find markers for the assessment of the respiratory sensitizing potential of inhaled compounds and mimicking the alveolar barrier to study cell-to-cell communication and inflammatory effects of nano particles at the air-liquid-interface.

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28. Oct. 2016

Evaluation of an in vitro screening model to assess phosgene inhalation injury

DOI: 10.1080/15376516.2016.1243183

Dorian S. Olivera, Heidi Hoard-Fruchey & Alfred M. Sciuto
Analytical Toxicology Devision, US Army, Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, USA

In vitro studies have clearly shown that phosgene exposure can have overwhelming effects on cellular homeostasis with regard to mediator release, activation of inflammatory pathways and compromised lung function.
In this study an in vitro exposure model with which to evaluate potential therapeutics for edemagenic gas inhalation exposure was tested.
Human bronchial epithelial (16HBE) cell cultures were exposed in the Vitrocell System to phosgene.
Transepithelial electrical resistance (TEER), Cellular viability assay (XTT assay), [14C]-Glutamine oxidation assay, Glucose metabolism assays and Lactate production measurements were taken.

 

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27. Oct. 2016

Usefulness of toxicological validation of VOCs catalytic degradation by airliquid interface exposure system

doi: 10.1016/j.envres.2016.10.027.

Margueritta Al Zallouha, Yann Landkocz, Julien Brunet, Renaud Cousin, Eric Genty, Dominique Courcot, Stéphane Siffert, Pirouz Shirali, Sylvain Billet
Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d′Opale, 189 A Avenue Maurice Schumann, 59140 Dunkerque, France


The exposure of A549 cells to VOCs and by-products at the Air-Liquid Interface exposure system were tested and showed toxic effects. Additionally a catalyst setup coupled to the Vitrocell system for testing by-products of toluene oxidation has been validated. The toxicological analysis is done with a cell viability test (LDH) and a TaqMan gene expression kit (RT-qPCR).

 

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23. Sep. 2015

Toxicological validation during the development of new catalytic systems using air / liquid interface cell exposure system

EUROTOX 13.-16. September 2015

Authors

Y. Landkocz1, M. Al Zallouha1, J. Brunet2, R. Cousin2, J.M. Halket3, E. Genty2, D. Courcot1, S. Siffert2, P. Shirali1, S. Billet1

(1) UCEIV, Chimie et Toxicologie des Emissions Atmosphériques, EA4492, Dunkerque, France; Sylvain.Billet@univ-littoral.fr
(2) UCEIV, Traitement Catalytique et Energie Propre, EA4492, Dunkerque, France
(3) Mass Spectrometry Facility, King’s College, London, United Kingdom

 

The aim of this study was to determine the most efficient catalyst for toluene oxidation. Thereby A549 lung cells were exposed using an air/liquid interface (ALI) system and the results are performed with the LDH cytotoxicity test and XME gene expression measurement.

The suitability of using Vitrocell® system as an innovative, direct and dynamic model of ALI exposure in the development of new catalysts is successfully shown.

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21. Jun. 2014

A model of human nasal epithelial cells adapted for direct and repeated exposure to airborne pollutants

Toxicology Letters

2014 Jun 21;229(1):144-149. doi: 10.1016/j.toxlet.2014.05.023. Epub ahead of print.

Authors
Bardet G.1, Achard S.2, Loret T.3, Desauziers V.4, Momas I.5, Seta N.6

1 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France; Agence de l'Environnement et de la Maîtrise de l'Energie, Angers, France.
2 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France.
3 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France.
4 Centre des Matériaux des Mines d'Alès, Ecole des Mines d'Alès, Pau, France.
5 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France
6 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France; AP-HP, Hôpital Bichat, Biochimie, Paris, France.

The exposure to gaseous pollutants at the air-liquid interface shows an effect on the respiratory tract. The repeated exposition enables a model that gets closer to physiologically relevant conditions. Inflammatory marker IL-6 and IL-8, cellular viability and cytokine production were assessed.

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2. Jun. 2014

Application of a modified gaseous exposure system to the in vitro toxicological assessment of tobacco smoke toxicants

Environmental and Molecular Mutagenesis

Volume 55, Issue 8, Article first published online: 2 JUN 2014 (doi: 10.1002/em.21876)

Authors
Damien Breheny1, Fiona Cunningham1, Joanne Kilford2, Rebecca Payne2, Deborah Dillon1 and Clive Meredith1

1 British American Tobacco, Group R&D, Southampton, Hampshire, United Kingdom
2 Covance Laboratories Ltd, North Yorkshire, United Kingdom

Demonstration of the suitability of the VC 10® exposure system for the assesment of the mutagenic potential of gases using the AMES Assay. Not only cigarette smoke, also a wide range of gaseous compounds and aerosol mixtures can be studied.

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