Advanced in vitro exposure systems.

30. Nov. 2020

Product News 12/2020

VITROCELL® 12/12 now available with Climatic Chamber

For reliable aerosol exposure of cell cultures using 12- or 24-well sized inserts

The VITROCELL® 12/12 module system has been designed to facilitate the exposure of mammalian cell cultures to airborne substances such as gases, complex mixtures, nanoparticles and fibers. It features a throughput of 3 dilution steps @ 3 replicates and 1 clean air control at 3 replicates. In order to optimize the exposure for liquid aerosols and to maximize the performance of humidified air supply it is now also available in a user-friendly climatic chamber.

VITROCELL® 12/12 now available with Climatic Chamber

Read more

Comments (0) Number of views (428)

2. Nov. 2020

Toxicological responses of BEAS-2B cells to repeated exposures to benzene, toluene, m-xylene, and mesitylene using air–liquid interface method

DOI: 10.1002/jat.4113


Clémence Méausoone1, Yann Landkocz1, Fabrice Cazier2, Marianne Seigneur1 Dominique Courcot1, Sylvain Billet1
1Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkirk, France
2Centre Commun de Mesures, Université du Littoral Côte d'Opale, Dunkirk, France

 

Benzene and toluene are solvents largely used in industries and emitted into the atmosphere, despite major and direct impacts on human health. This study shows the feasibility of observing mechanisms of toxic action during repeated exposure at ALI to doses close to human exposure. The second strength of this study was the measure of XME induction level with the initiation of several xenobiotic metabolism pathways over time. BEAS-2B cells were exposed to benzene, toluene, m-xylene, or mesitylene gaseous stream diluted in air using the Vitrocell ALI system.

 

Read more

Comments (0) Number of views (55)

2. Nov. 2020

Influence of the environmental relative humidity on the inflammatory response of skin model after exposure to various environmental pollutants

https://doi.org/10.1016/j.envres.2020.110350

Emeline Seurat a, Anthony Verdin b, Fabrice Cazier c, Dominique Courcot b, Richard Fitoussi d, Katell Vi´e d, Val´erie Desauziers e, Isabelle Momas a, Nathalie Seta a, Sophie Achard a
a Laboratoire de Sant´e Publique et Environnement, Hera “Health Environmental Risk Assessment”, Inserm UMR1153-CRESS (Centre de Recherche en Epid´emiologie et StatistiqueS), Universit´e de Paris, Facult´e de Pharmacie de Paris, 4, Avenue de L’Observatoire, 75006, Paris, France
b Unit´e de Chimie Environnementale et Interactions sur le Vivant UR4492, SFR Condorcet FR CNRS 3417, Maison de La Recherche en Environnement Industriel 2, Universit´e Du Littoral Cˆote D’Opale, 189A Avenue Maurice Schumann, 59140, Dunkerque, France
c Centre Commun de Mesures (CCM), Universit´e Du Littoral-Cˆote D’Opale, 145 Avenue Maurice Schumann, 5914, Dunkerque, France
d Laboratoires Clarins, 5 Rue Amp`ere, 95300, Pontoise, France
e IPREM, IMT Mines Ales, Universit´e de Pau et des Pays de L’Adour, E2S UPPA, CNRS, Pau, France

 

In the course of this study, they tested various pollutants with different chemical compositions, applying them to the apical side of Reconstructed Human Epidermis and being particularly interested in the effect relative humidity has on the reaction to pollutants. Investigating several cytokines and chemokines, they showed that IL-1α, IL-6, IL-8, and RANTES are the cytokines/chemokines almost systematically induced by most pollutants.

 

Read more

Comments (0) Number of views (110)

8. Oct. 2020

Alternative air–liquid interface method for inhalation toxicity testing of a petroleum-derived substance

https://doi.org/10.1016/j.mex.2020.101088


Verstraelen Sandra a , Jacobs An a , Van Laer Jo a , Van Deun Masha b , Bertels Diane b , Hilda Witters a , Remy Sylvie a , c , Geerts Lieve a , Deferme Lize d , Frijns Evelien a
a VITO NV (Flemish Institute for Technological Research), Unit HEALTH, Mol, Belgium 
b VITO NV, Unit SCT (Separation and Conversion Technology), Mol, Belgium 
c Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium 
d ExxonMobil Petroleum and Chemical B.V., Machelen, Belgium

 

A549 cells were exposed to gasoline at the ALI by passive dosing, an approach that is already used in aquatic toxicity testing. This showes a clear dose-dependent biological response. This in vitro -based new approach methodology might be promising for inhalation toxicity testing of (semi-)volatile complex substances.

 

Read more

Comments (0) Number of views (50)

29. Sep. 2020

Invited review: human air-liquid-interface organotypic airway tissue models derived from primary tracheobronchial epithelial cells—overview and perspectives

https://doi.org/10.1007/s11626-020-00517-7


Xuefei Cao1, Jayme P. Coyle2, Rui Xiong1, Yiying Wang1, Robert H. Heflich1, Baiping Ren1, William M. Gwinn3, Patrick Hayden4, Liying Rojanasakul2

1 Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, US Food and Drug Administration, 3900 NCTR Rd., AR Jefferson, USA
2 Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers forDisease Control and Prevention,Morgantown,WV, USA
3 Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Durham, NC, USA
4 BioSurfaces Inc., Ashland, MA, USA


One important element for validating any new assay for making regulatory decisions is determining its performance relative to an accepted standard. Conducting in vivo inhalation toxicity studies using whole-body or nose-only exposure systems is expensive and time-consuming and typically requires a large number of animals. The goal of using alternative methods, like human in vitro ALI airway cultures, ultimately is to replace inhalation toxicity testing in animals with in vitro approaches. Transition from animal- to human-based models is ultimately expected to lead to faster and better predictive toxicity assessments and therapeutic development at lower cost.  This study shows the development and validation of alternative in vitro methods for acute toxicity testing, including acute inhalation toxicity testing.
 

Read more

Comments (0) Number of views (88)

24. Mar. 2020

Comparison of Vapor and Liquid Phase Acrolein Exposures to Air Liquid Interface (ALI) Cell Cultures

David H. Brandwein, F. Adam Bettmann, Michael P. DeLorme, Alan T. Eveland, Lawrence M. Milchak 
3M Corporate Toxicology and Environmental Science, St. Paul, MN
 

The STL is working to develop an in vitro screening ALI model to assess the acute respiratory irritation potential for new chemicals. These experiments examined multiple aspects of the model, including different cell culture systems (A549 and EpiAirway), different exposure methods (dynamic vapor and liquid phase), and different post exposure periods, all using acrolein as a model respiratory irritant. The goal was to better understand the critical parameters of the cell systems and exposure methods to enable the development of a consistent screening model, while gaining clarity of the dosimetry. 

Read more

Comments (0) Number of views (852)

10. Mar. 2019

New Products at VITROCELL® User Group Meeting 2019

09 March 2019, Hilton Inner Harbour Hotel, Baltimore, USA

Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

Read more

Comments (0) Number of views (587)

11. Mar. 2018

A setup to test toxicity of alcohol inhalation from consumer products

User Group Meeting 2018

Dr. Monique Manche1, Dr. Fabrice Nesslany2, Tobias Krebs3
1Laboratoires Anios, 59260 Lille-Hellemmes, France
2Laboratoire de toxicologie génétique, Institut Pasteur de Lille, 59000 Lille, France
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

Genotoxicity tests (in vitro micronucleus test and comet assay) have been conducted on p53 competent pulmonary cells, with alcohol administration as vapours to obtain data under conditions more representative of actual exposure conditions via the pulmonary route.

 

Read more

Comments (0) Number of views (795)

11. Mar. 2018

Update on FTIR integration

User Group Meeting 2018

Dr. Martin Kieninger1, Tobias Krebs2

1ANSYCO GmbH, Gasmet Group, 76131 Karlsruhe, Germany

2VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

The poster shows a reliable, easy to use online analysis tool with fast response and a wide range of components due to Calcmet library. Ideal for quality control and dose monitoring.


Read more

Comments (0) Number of views (652)

11. Mar. 2018

VITROCELL® 6/48: Changeover set for 24-well sized inserts

User Group Meeting 2018

Tobias Krebs

VITROCELL Systems GmbH, 79183 Waldkirch, Germany 

 

The poster shows the VITROCEL® 48 exposure system with a 6- and a 24-well setup.

Read more

Comments (0) Number of views (500)
RSS
12345
Back to Top