DOI:10.3791/61210 

Hedwig M. Braakhuis^₁, Ruiwen He^1,2, Rob J. Vandebriel¹, Eric R. Gremmer¹, Edwin Zwart¹, Jolanda P. Vermeulen¹, Paul Fokkens¹, John Boere¹, Ilse Gosens¹, Flemming R. Cassee^1,2 
1National Institute for Public Health and the Environment (RIVM) 
²Institute for Risk Assessment Sciences (IRAS) 

This article provides a method for culturing and exposing the human bronchial epithelial cell line Calu-37 at the Air-liquid Interface that mimics realistic, repeated inhalation exposure conditions that can be used for toxicity testing. By applying a continuous airflow using the Automated Exposure System, the cell model can be exposed to a low concentration of particles over a longer time period, reflecting realistic exposure conditions. Characteristics of both the cell model and of  the exposure system are essential for achieving a realistic inhalation exposure model that can be used for repeated exposures. 

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Autoren:
Hillemann, L.; Stintz, M.; Streibel, T.; Zimmermann, R.; Öder, S.; Kasurinen, S.; di Bucchianico, S.; Kanashova, T.; Dittmar, G.; Konzack, D.; Große, S.; Rudolph, A.; Berger, M.; Krebs, T.; Saraji-Bozorgzad, M. R.; Walte, A.

Die Wirkung von Emissionen aus dem Trennen von Carbonbeton werden in einer Kombination aus Trennstaubgenerator, Verdünnungssystem und Expositionssystem an Zellkulturen (A549 und THP-1) gegenüber den entstehenden Stäuben bestimmt (Zytotoxizität).

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Nala Rogers for 'Inside Science'

A report about artificial lung reveals surprising dangers from pellet burners and diesel-fueled ships at the University of Rostock and Helmholtz Zentrum München in Germany.

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DOI:10.1371/journal.pone.0157964

Sean C. Sapcariu^1,12, Tamara Kanashova^2,12, Marco Dilger^3,4,12, Silvia Diabaté^3,12, Sebastian Oeder^5,6,12,13, Johannes Passig^7,12, Christian Radischat^7,12, Jeroen Buters^5,6,12,13, Olli Sippula^8,12, Thorsten Streibel^7,9,12, Hanns-Rudolf Paur^4,12, Christoph Schlager^4,12, Sonja Mülhopt^4,12, Benjamin Stengel^10,12, Rom Rabe^10,12, Horst Harndorf^10,12, Tobias Krebs^11,12, Erwin Karg⁹, Thomas Gröger⁹, Carsten Weiss^3,12, Gunnar Dittmar^2,12, Karsten Hiller^1,12, Ralf Zimmermann^7,9,12

¹ Luxembourg Centre for Systems Biomedicine 6, avenue du Swing, L-4362 Esch-sur-Alzette, Luxembourg,
² Mass Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine Berlin-Buch, Berlin, Germany,
³ Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
⁴ Institute for Technical Chemistry (ITC), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
⁵ Center of Allergy and Environment (ZAUM), Helmholtz Zentrum München and Technische Universität München, Munich, Germany,
⁶ CK-CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland,
⁷ Joint Mass Spectrometry Centre, Division of Analytical and Technical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany,
⁸ University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland,
⁹ Joint Mass Spectrometry Centre, CMA – Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany,
¹⁰ Chair of Piston Machines and Internal Combustion Engines, University Rostock, Rostock, Germany,
¹¹ Vitrocell GmbH, Waldkirch, Germany,
¹² HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany; Kuopio, Finland; Cardiff, United Kingdom; Esch-Belval, Luxembourg,
¹³ German Center for Lung Research (DZL), Munich, Germany

An automated ALI exposure system station with 18 exposure positions was used as the interface for mouse macrophage RAW 264.7 cell line exposures of the diesel engine exhaust. The evaluation is devided in LDH release assay, Metabolite extraction and GC-MS processing, Stable isotope labeling by amino acids in cell culture (SILAC), Proteome extraction and LC-MS/MS analysis of peptides and Proteomics Data An

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doi:10.1016/j.jaerosci.2016.02.005

Sonja Mülhopt ^{a, i}, Marco Dilger ^{a, b, i}, Silvia Diabaté ^{b, i}, Christoph Schlager ^{a, i}, Tobias Krebs ^{c, i}, Ralf Zimmermann ^{d, h, i}, Jeroen Buters ^{e, i}, Sebastian Oeder ^{e, g, i}, Thomas Wäscher ^f, Carsten Weiss ^{b, i}, Hanns-Rudolf Paur ^{a, i}, 

^a Karlsruhe Institute of Technology, Institute for Technical Chemistry, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
^b Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
^c Vitrocell Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany
^d University of Rostock, Institute of Chemistry, Dr.-Lorenz-Weg 1, 18051 Rostock, Germany
^e Center of Allergy & Environment (ZAUM), Technische Universität and Helmholtz Zentrum München, Biedersteiner Str. 29, 80802 München, Germany
^f Ingenieurbüro für Energie- und Verfahrenstechnik, Von-Dalheim-Str. 2, 69231 Rauenberg, Germany
^g Kühne Foundation, Christine Kühne Center for Allergy Research and Education (CK- CARE), München, Germany
^h Cooperation Group “Comprehensive Molecular Analytics” – CMA, Helmholtz Zentrum München, 85764 Oberschleißheim/ Germany
ⁱ HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health

• We present a fully automated Air-Liquid-Interface Exposure System.
• System characterisation shows a high reproducibility.
• Wood combustion aerosol causes cytotoxicity in human lung epithelial cells.
• Gene regulation caused by ship diesel emissions in human lung epithelial cells.

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