Advanced in vitro exposure systems.

15. Mar. 2017

New Products 2017 presented at VITROCELL® User Group Meeting

10 March 2017, Hilton Baltimore Inner Harbor Hotel, USA

Following the VITROCELL® User Group Meeting 2016 in New Orleans, international scientists from Canada, Europe, Japan, Korea and USA reconvened in March 2017.

The meeting offered an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange experiences in working with VITROCELL® devices and to meet fellow researchers. It was held prior to the International Society of Toxicology 56th Annual Meeting and ToxExpo – one of the largest international conferences related to toxicology.

Focus of the event was to exchange latest research results, to give an update on VITROCELL’s participation in major European and German research programs as well as the presentation of new products for 2017.

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1. Mar. 2017

Characterization and Application of the VITROCELL VC1 Smoke Exposure System and 3D EpiAirway Models for Toxicological and e-Cigarette Evaluations

DOI: 10.1089/aivt.2016.0035
Wanda Fields,1 Anna Maione,2 Brian Keyser,1 and Betsy Bombick1

1RAI Services Company, Scientific and Regulatory Affairs, Winston-Salem, North Carolina.
2MatTek Corporation, Ashland, Massachusetts.

The aim of this study was to assess the effects of aerosol from two reference combustible cigarettes and two e-cigarettes by using the VC1 smoking machine with the air-liquid interface and to evaluate donor-to-donor variability of three EpiAirway tissue donors with the endpoints viability, barrier integrity, and gene promoter/expression regulation.


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31. Jan. 2017

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

DOI: 10.1089/aivt.2016.0025

Jason Adamson1,* Xiang Li2,* Huapeng Cui2, David Thorne1, Fuwei Xie2, and Marianna Gaca1
1British American Tobacco, R&D, Southampton, United Kingdom.
2Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, PR China.
*Joint first authors.

A VC 10 Smoking Robot was designated to look at the generated aerosol nicotine concentration from different e-cigarettes compared with the 3R4F reference cigarette in two different laboratories. The aerosol was trapped and the nicotine concentration was analysed by different kinds of mass spectometry.
Nicotine assessment across the tested e-cigarette categories showed consistent delivery of nicotine per puff within products and that the method was sensitive enough to detect different levels of nicotine across products. Good overlap in nicotine results were obtained in the two different laboratories.

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3. Jan. 2017

Development, qualification, validation and application of the neutral red uptake assay in Chinese Hamster Ovary (CHO) cells using a VITROCELL® VC10® smoke exposure system

Wanda Fields a, Kathy Fowler a, Victoria Hargreaves b, Lesley Reeve b, Betsy Bombick a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street,Winston-Salem, NC 27101, USA
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG1 3PY, UK

This study discribes the development, validation and application of the NRU assay in Chinese Hamster Ovary (CHO) cells, following exposure to fresh whole smoke generated with the VITROCELL®VC10®system. The VITROCELL® VC10® successfully provided consistent generation and delivery of whole smoke from a cigarette (3R4F) and a primarily “heat-not-burn” cigarette (Eclipse).

 

 

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7. Dec. 2016

The comparative in vitro assessment of e-cigarette and cigarette smoke aerosols using the gH2AX assay and applied dose measurements

http://dx.doi.org/10.1016/j.toxlet.2016.12.006

David Thorne, Sophie Larard, Andrew Baxter, Clive Meredith, Marianna Gaҫa

British American Tobacco, R&D Centre, Southampton, Hampshire, SO15 8TL, UK

 

H I G H L I G H T S
Cigarette smoke generates a dose dependent increase in double strand breaks.
E-cigarette aerosols did not generate any increases in double strand breaks above control levels.
E-cigarette exposures were approximately 28-fold greater than that of cigarette smoke exposures.
In vitro dosimetry ensures accurate interpretation of the data, and confirms delivery of test agent.

In vitro dosimetry allows cross-product, study and system comparisons.

 

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9. Oct. 2016

The mutagenic assessment of electronic-cigarettes and tobacco smoke using the Ames assay in strains TA98 and TA100

CORESTA Congress 2016. 9-13 October 2016 - Berlin, Germany

CORESTA Congress, 9-13th October 2016, Germany
Poster No:STPOST34

 

Thorne, D1., Crooks, I1., Hollings, M2., Seymour, A2., Meredith, C1., Gaa, M1.

1 British American Tobacco, R&D Centre, Southampton, SO15 8TL, UK

2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK.

 

The Aim of this study was to assess the mutagenicity of an e-cigarette aerosol, compared to cigarette smoke in tester strains TA98 and TA100 using two different exposure matrices, TPM/eTPM (or ACM – aerosol collected matter) and whole aerosol. A Vitrocell® VC 10 Smoking Robot was used to generate aerosol streams from a traditional reference cigarette (3R4F) and e-cigarette (Vype® ePen). 

 

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12. Jul. 2015

Development of an in vitro cytotoxicity model for aerosol exposure using 3D reconstructed human airway tissue; application for assessment of e-cigarette aerosol

DOI:10.1016/j.tiv.2015.05.018

Authors

Louise Neilsona, Courtney Mankusb, David Thornea, George Jacksonb, Jason DeBayb, Clive Mereditha

a British American Tobacco, Group Research and Development, Regents Park Road, Southampton, Hampshire SO15 8TL, United Kingdom
b MatTek Corporation, 200 Homer Avenue, Ashland, MA 01721, United States

 

The presentation of a new highly differentiated 3D airway culture, grown and exposed in the VITROCELL® system, shows an successful compatibility of the tissue and the VITROCELL® system for testing e-cigarettes. The results are shown with the MTT assay for cell viability and measurement of TEER.

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15. Jun. 2015

Presentation of new Smoking Machine VITROCELL VC 10® S-TYPE

Cutting edge technologies make the VITROCELL® smoking machine a perfect tool for evaluation of conventional and e-cigarettes.

This smoking machine features all special requirements for high-class research: compliance with ISO, Health Canada and square puff profile protocols as well as ease of handling, fast cleaning and high speed product change capabilities.

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2. Jun. 2015

VITROCELL User Group Meeting 2015

Presentation of new VITROCELL® products and experience exchange among international scientists from Europe, Japan, Korea and USA.

The focus of the event was to present new routes for dosimetry, to give an update on VITROCELL’s participation in major European research programs related to nanoparticles as well as the presentation of new products for 2015.

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22. May. 2015

Testing particle delivery of e-cigarettes

VITROCELL® Application Note for VC 10® S-TYPE Smoking Machine and Particle Photometers

The new application note shows a successfull monitoring of the particle delivery of different e-cigarettes on the setup of a VITROCELL VC 10® S-TYPE Smoking Machine combined with the VITROCELL® Particle Photometer and Monitor Software.

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