Advanced in vitro exposure systems.

11. Mar. 2018

Characterization of a Vitrocell VC1 Using Nicotine Dosimetry: An Essential Component Towards Standardised In Vitro Aerosol Exposure of Tobacco and Next Generation Nicotine Delivery Products

IIVS at SOT 2018

D. Sheehan1, M. Aragon1, J. Adamson2, M. Gaca2, H. Raabe1, and H. Behrsing1.
1Institute for In Vitro Sciences, Inc., Gaithersburg, MD; and 2British American Tobacco, Southampton, United Kingdom.

This study demonstrates successful Vitrocell VC1 aerosol generation and delivery across multiple nicotine product categories (3R4F cigarette smoke, e-liquid and THP), as characterised using nicotine as a dosimetry marker. The data suggests the VC1 can reproducibly generate and deliver tobacco product and NGP aerosols for future in vitro assessment and matches the performance of reported exposure systems.

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11. Mar. 2018

VITROCELL® 6/48: Changeover set for 24-well sized inserts

User Group Meeting 2018

Tobias Krebs

VITROCELL Systems GmbH, 79183 Waldkirch, Germany 

 

The poster shows the VITROCEL® 48 exposure system with a 6- and a 24-well setup.

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11. Mar. 2018

VITROCELL® VC 1 Smoking Machine with Shisha Option

User Group Meeting 2018

Markus Berger, Marco Hebestreit

VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The Poster shows a demonstration of the VC 10 smoking machine with Shisha option

 

 

 

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11. Mar. 2018

New Products at VITROCELL® User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2018.

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11. Mar. 2018

The use of human 3D reconstructed bronchial tissue to study the effects of cigarette smoke and e-cigarette aerosol on a wide range of cellular endpoints

SOT 57th Annual Meeting, 11 –15th March 2018

Lukasz Czekala1, Matthew Stevenson1, Liam Simms1, Nicole Tschierske1, Anna G. Maione2, Tanvir Walele3
1 Imperial Tobacco Ltd, 121 Winterstoke Road, Bristol, BS3 2LL UK
2 MatTek, 200 Homer Ave., Ashland, MA, USA
3 Fontem Ventures B.V., an Imperial Brands PLC Company, Radarweg60, 1043 NT Amsterdam

A 3D in vitro organotypic model of the human respiratory epithelium was cultured at the Air-Liquid Interface (ALI) and exposed to cigarette smoke and e-cigarette aerosol, generated by using a VITROCELL® VC1 smoking machine under Health Canada Intense and CORESTA conditions.
The results are shown in tissue viability (MTT), barrier integrity (TEER), Barrier function, tissue secretion of the pro-inflammatory cytokines (IL-6 and IL-8). The presence of 8-isoprostane is used as an indicator of oxidative stress and antioxidant deficiency. Additionally the tissue morphology was assessed by H&E staining and immunofluorescent staining was conducted for specific markers.


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1. Mar. 2018

Comparative biological impacts of an aerosol from carbon-heated tobacco and smoke from cigarettes on human respiratory epithelial cultures: A systems toxicology assessment

https://doi.org/10.1016/j.fct.2018.02.063
Anita R. Iskandar, Florian Martin, Patrice Leroy, Walter K. Schlage, Carole Mathis, Bjorn Titz, Athanasios Kondylis, Thomas Schneider, Grégory Vuillaume, Alain Sewer, Emmanuel Guedj, Keyur Trivedi, Ashraf Elamin, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng
PMI R&D, Philip Morris Products S.A., Part of Philip Morris International group of companies, Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland


Highlights
• Aerosols of a heat-not-burn tobacco product elicited lower impacts compared with smoke of 3R4F reference cigarettes.
• Systems toxicology revealed changes at the cellular–molecular levels not otherwise apparent from morphological features.
• The reduced impact of heated tobacco aerosols on small airway was similar than that on nasal epithelial cultures.

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18. Jan. 2018

Extreme testing of undiluted e-cigarette aerosol in vitro using an Ames air-agar-interface technique

https://doi.org/10.1016/j.mrgentox.2018.01.008

D. Thornea, M. Hollingsb, A. Seymourb, J. Adamsona, A. Dalrymplea, M. Ballantyneb, M. Gacaa

a British American Tobacco, R&D, Southampton, Hampshire, SO15 8TL, UK
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

This study investigated the in vitro mutagenicity of e-cigarettes and designed a study to deliver e-cigarette aerosol on an undiluted per puff basis under extreme testing conditions, using the Ames assay across a broader range of bacterial tester strains. The Vitrocell VC 10 smoking robot, previously used to generate and dilute cigarette smoke aerosols was modified to enable the testing of an undiluted e-cigarette aerosol.

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15. Jan. 2018

An approach to testing undiluted e-cigarette aerosol in vitro using 3D reconstituted human airway epithelium

https://doi.org/10.1016/j.tiv.2018.01.010
E. Bishop, L. Haswell, J. Adamson, S. Costigan, D. Thorne, M. Gaca
British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK

A reconstituted 3D human airway epithelium was exposed to undiluted aerosols at the air-liquid interface, using a Vitrocell VC 10 under ISO and HCI conditions. A 3R4F reference cigarette and a commercially available e-cigarette product were tested on their effect on CBF and TEER.

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3. Jun. 2017

Delivery of glycerol-based aerosols in the Vitrocell® 24/48 aerosol exposure system

ISAM, THE 21ST INTERNATIONAL SOCIETY OF AEROSOLS IN MEDICINE CONGRESS, 03-07 June, 2017

Sandro Steiner, Shoaib Majeed, Gilles Kratzer, Mounir Rhouma, Quentin Dutertre, Grégory Vuillaume, Arno Knorr, Julia Hoeng, Stefan Frentzel

Philip Morris International R&D, Philip Morris Products S.A.

Characterization of the Vitrocell® 24/48 aerosol exposure system for its use in conjunction with liquid aerosols shows a high feasibility for an easy detection of aerosol deposition.

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3. Jun. 2017

In Vitro Testing of Aerosolized Substances with Primary 3D Human Airway Epithelia: Application of ALICE-Cloud Technology to e-liquids (e-cigarettes)

ISAM, THE 21ST INTERNATIONAL SOCIETY OF AEROSOLS IN MEDICINE CONGRESS, 03-07 June, 2017

S. Constant1, A. Stelzl2,3, O. Eickelberg2,3,4, O. Schmid2,3
1Epithelix, Genève, Switzerland
2Comprehensive Pneumology Center (CPC-M), Member of the German Center for Lung Research (DZL), Munich, Germany
3Institute of Lung Biology and Disease, Helmholtz Zentrum München, Munich/Neuherberg, Germany
4University Hospital of the Ludwig Maximilians University (LMU), Munich, Germany

The poster shows the ALICE-Cloud technology combined with primary ALI 3D cell cultures. The effect of nebulized e-liquids were investigated in terms of tissue integrity, celia beating frequency, mucin secretion and IL-8 secretion.

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