Brian M. Keyser¹, John Wertman¹, Michael Hollings², Robert Bedford², and Kristen Jordan^1
1 Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC; 
² Labcorp Early Development Laboratories Ltd., Harrogate, UK

In this study whole smoke from a marketed combustible cigarette and whole aerosol from four different ENDS were evaluated on cell viability and Nrf2 response in a 3D human airway model transfected with a luciferase Nrf2 promoter.
Whole aerosol was generated using a Vitrocell ® VC10® Smoke Exposure System. The tissues were exposed to whole smoke from a market combustible generated under Health Canada Intense regime.These data show that the 3D Nrf2 EpiAirway™ in vitro model can be used to assess and discriminate responses from a biomarker (oxidative stress) for disease pathways associated with tobacco product usage.

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Thomas Shutsky¹, Brian M. Keyser¹, Kristen Jordan¹, Michael Hollings², and Emma Rothwell^2
1Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC; 
²Labcorp Early Development Laboratories Ltd., Harrogate, UK

In this study, EpiAirway™ tissues were exposed to whole aerosol/smoke generated from six different Vuse Alto electronic nicotine delivery system test articles with various nicotine concentrations and a combustible cigarette. A Vitrocell® VC10® robot was used to generate whole aerosol/smoke with either a modified ISO or Health Canada Intense smoking regimen. Whole smoke/aerosol was puffed with clean air with a series of different air flows to achieve the delivered dose range. 

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Regular leak tests are mandatory in aerosol research. A leak caused by a forgotten connection or defective o-ring may have a significant influence on the aerosol exposure process. We recommend to carry out a leak test prior to the experiment and as part of cleaning or service routine.

VITROCELL Application Note

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https://doi.org/10.1016/j.toxlet.2020.11.006

Albert Giralt^a, Anita R Iskandar^a, FlorianMartin^a, ElisaMoschini^b, TomassoSerchi^b, AthanasiosKondylis^a, DiegoMarescotti^a, PatriceLeroy^a, LauraOrtega-Torres^a, ShoaibMajeed^a, CelineMerg^a, KeyurTrivedi^a, EmmanuelGuedj^a,StefanFrentzel^a, Nikolai V Ivanov^a, Manuel C Peitsch^a, Arno C Gutleb^b, JuliaHoeng^a

^a Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000, Neuchâtel, Switzerland
^b Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Luxembourg

Highlights
• Biological impact of exposure to an aerosol of an e-vapor device was investigated using human bronchial and alveolar models.
• The biological impact of e-vapor aerosol exposure was lower than that of cigarette smoke at similar nicotine levels.
• The exposure effects at the molecular levels were evaluated using a systems toxicology approach.

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https ://doi.org/10.1186/s1293 1-020-01539-1

Rakeysha Pinkston^1,2, Hasan Zaman², Ekhtear Hossain², Arthur L. Penn² and Alexandra Noël^2
1 Department of Environmental Toxicology, College of Sciences and Engineering, Southern University and A&M College, Baton Rouge, LA 70813, USA. 
² Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA.

There are thousands of flavors and flavoring combinations of e-liquids on the market with the potential to produce harmful effects when aerosolized through an ENDS device. While more research is needed regarding the potential toxicity associated with inhaling flavoring additives in combination with nicotine salt for future regulation of ENDS products, the present study provides laboratory-based evidence that should be considered regarding regulation of nicotine salt-based products.

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