Vitrocell Systems GmbH - e-cigarettes

7. Sep. 2019

Application of a multi‑layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e‑liquid and its corresponding aerosol using an e‑cigarette device

https://doi.org/10.1007/s00204-019-02565-9

Anita R. Iskandar, Filippo Zanetti, Diego Marescotti, Bjorn Titz, Alain Sewer, Athanasios Kondylis, Patrice Leroy, Vincenzo Belcastro, Laura Ortega Torres, Stefano Acali, Shoaib Majeed, Sandro Steiner, Keyur Trivedi, Emmanuel Guedj, Celine Merg, Thomas Schneider, Stefan Frentzel, Florian Martin, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

Previous experimental setups shows the effects of e-liquids on cell viability (first layer), followed by investigating the potential mechanisms of toxicity elicited by e-liquids (second layer) and finally assessing the impacts of aerosols (third layer). In this present work shows how the three-layer framework is leveraged to evaluate the potential toxicity and biological effects of the MESH Classic Tobacco and Base e-liquids/aerosols compared with those of 3R4F CS.

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Categories: Publications

Tags: inhalation exposure, tobacco smoke, e-cigarettes, VITROCELL 24, air-liquid Interface

11. Aug. 2019

Exposure to aerosols from electronic cigarettes using the MESH™ technology has a reduced biological impact on bronchial epithelial cell cultures compared with exposure to cigarette smoke

Gordon Research Conference, Integration of Emerging Technologies in Mechanistic and Translational Toxicology,Andover, August 11–16, 2019

Albert Giralt, Florian Martin, Anita R. Iskandar, Alain Sewer, Laura Ortega Torres, AthanasiosKondylis, Patrice Leroy, Celine Merg, ShoaibMajeed, Emmanuel Guedj, Thomas Schneider, KeyurTrivedi, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng

PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud5, CH-2000 Neuchâtel, Switzerland

In contrast to 3R4F CS exposure, exposure to IQOS MESH™ Classic Tobacco aerosols did not cause tissue damage or have an impact on ciliary beating functionality in bronchial epithelial cell cultures despite resulting in greater concentrations of deposited nicotine. Cultures exposed to IQOS MESH™ Classic Tobacco aerosols showed fewer changes in proteins involved in xenobiotic metabolism than those exposed to CS.

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Categories: Publications

Tags: inhalation exposure, tobacco smoke, e-cigarettes, VITROCELL 24, air-liquid Interface

10. Mar. 2019

New Products at VITROCELL® User Group Meeting 2019

09 March 2019, Hilton Inner Harbour Hotel, Baltimore, USA

Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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10. Mar. 2019

Exposure to an aerosol from a novel electronic cigarette using the MESH™ technology elicited reduced biological impacts than exposure to cigarette smoke on buccal and small airway epithelial cultures

Society of Toxicology, Annual Meeting 2019, Baltimore, MD, USA | 10-14 March

Anita R. Iskandar, Filippo Zanetti, Athanasios Kondylis, Florian Martin, Alain Sewer, Laura Ortega Torres, Shoaib Majeed, Sandro Steiner, Emmanuel Guedj, Celine Merg, Thomas Schneider, Keyur Trivedi, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng

PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

The impacts of an acute exposure to cigarette smoke (CS) and to aerosol from a novel electronic cigarette (EC) device using MESH™ technology were assessed using human organotypic buccal epithelial cultures and small airway epithelial cultures. A paired design was implemented: in parallel to the exposure to CS or EC aerosol, cultures were also exposed to air in the same exposure module. Tissue damage was not seen in cultures exposed to the IQOS MESH™ Classic Tobacco aerosol despite resulting in greater concentrations of deposited nicotine. In buccal cultures, CS and IQOS MESH™ Classic Tobacco aerosol elicited different infammatory response.

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Categories: Publications

Tags: inhalation exposure, tobacco smoke, e-cigarettes, VITROCELL 24, air-liquid Interface

5. Mar. 2019

A lower impact of an acute exposure to electronic cigarette aerosols than to cigarette smoke in human organotypic buccal and small airway cultures was demonstrated using systems toxicology assessment

DOI:10.1007/s11739-019-02055-x
Filippo Zanetti¹, Athanasios Kondylis¹, Florian Martin¹, Patrice Leroy¹, Shoaib Majeed¹, Sandro Steiner¹, Yang Xiang¹, Laura Ortega Torres¹, Keyur Trivedi¹, Emmanuel Guedj¹, Celine Merg¹, Stefan Frentzel1, Nikolai V. Ivanov¹, Utkarsh Doshi², Kyeonghee Monica Lee², Willie J. McKinneyJr², Manuel C. Peitsch¹, Julia Hoeng¹

¹ Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
² Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

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Categories: Publications

Tags: inhalation exposure, tobacco smoke, e-cigarettes, VITROCELL 24, air-liquid Interface

2. Feb. 2019

Toxicological comparison of cigarette smoke and e-cigarette aerosol using a 3D in vitro human respiratory model.

DOI: 10.1016/j.yrtph.2019.01.036

Czekala L¹, Simms L², Stevenson M², Tschierske N², Maione AG³, Walele T².
¹Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK. Electronic address: Lukasz.Czekala@uk.imptob.com.
² Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK.
³ MatTek, 200 Homer Ave., Ashland, MA, USA.

In this research article a respiratory epithelial model, EpiAirway, was used to examine the biological effects of nicotine-containing blu PLUS + e-cigarettes, in comparison to conventional cigarette smoke. Tissues were exposed at the air-liquid interface to cigarette smoke or e-cigarette aerosol generated using a VITROCELL VC1 smoking/vaping robot.

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Categories: Publications

Tags: tobacco smoke, e-cigarettes, VC 1 smoking machine, air-liquid Interface

28. Oct. 2018

Big tobacco embraces in vitro toxicology

Manufacturers invest in nonanimal tests to evaluate health risks of e-cigarettes and other next-generation tobacco products

c&en, Volume 96, Issue 43

Britt E. Erickson

The complexity of next-generation tobacco products has increased by a number of factors since FDA was given responsibility to regulate tobacco. Testing e-liquids with animals is not practical, economically and not that accurate. The Vitrocell system generates airborne materials from test products, such as smoke from reference cigarettes and vapor from e-cigarettes, in a manner that simulates how a person would puff. This artcile shows, how IIVS scientists use human donor tissues, obtained from organs, to create cell cultures and in vitro test systems.

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Categories: Publications

Tags: tobacco smoke, e-cigarettes, VITROCELL 24, VC 10 smoking robots, VITROCELL 12, air-liquid Interface, airborne particles

3. May. 2018

Application of a direct aerosol exposure system for the assessment of biological effects of cigarette smoke and novel tobacco product vapor on human bronchial epithelial cultures

https://doi.org/10.1016/j.yrtph.2018.05.004

Shinkichi Ishikawa∗, Kazushi Matsumura, Nobumasa Kitamura, Kanae Ishimori, Yuichiro Takanami, Shigeaki Ito

Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2 Umegaoka, Aoba-ku, Yokohama, Kanagawa 227-8512, Japan

The experimental setup for the aerosol exposure consisted of a VC 10 smoking robot, a dilution system, and a Vitrocell 12/3 glass module the exposure of nicotine and TPM in the mainstream aerosols from 3R4F and novel tobacco vapor on MucilAir™ cells. The results are shown in cytotoxicity, IL-8, IL-1ß and gene expression.

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Categories: Publications

Tags: inhalation exposure, tobacco smoke, e-cigarettes, VC 10 smoking robots, VITROCELL 12

5. Apr. 2018

A Case Study for the Comparison of In Vitro Data Across Multiple Aerosol Exposure Studies with Extrapolation to Human Dose

DOI: 10.1089/aivt.2017.0042

David Thorne, Emma Bishop, Linsey Haswell, and Marianna Gaca
British American Tobacco Group R&D, Southampton, United Kingdom

This case study has demonstrated that dosimetry techniques using particulate markers can allow in vitro biological data generated on five independent aerosol exposure system studies, under contrasting experimental designs and with different cell types, to be directly compared.

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Categories: Publications

Tags: inhalation exposure, tobacco smoke, e-cigarettes, VC 10 smoking robots, VITROCELL 6, microbalance, VITROCELL 12, VC 1 smoking machine, air-liquid Interface

28. Mar. 2018

Characterization of a Vitrocell VC1 Using Nicotine Dosimetry: An Essential Component Toward Standardized In Vitro Aerosol Exposure of Tobacco and Next Generation Nicotine Delivery Products

DOI: 10.1089/aivt.2018.0001
Holger Behrsing,¹Mario Aragon,¹ Jason Adamson,² Devin Sheehan,¹ Marianna Gaca,² Rodger Curren,¹ and Erin Hill¹
¹Respiratory Toxicology, Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.
²R&D, British American Tobacco, Southampton, United Kingdom.

The study shows an assessment of VC1 aerosol generation that was conducted by measuring puff-by-puff in vitro nicotine concentration of 3R4F cigarettes (ISO and HCI regimes), e-cigarette (CRM81 puffing profile), and THP (HCIm regime). The aerosols were applied to in vitro or ex vivo cellular and tissue systems that model the respiratory tract, such as reconstructed human airways (RHuA), which are grown at the air–liquid interface (ALI). Additionally a comparison between the Vitrocell VC1 (U.S. Laboratory) and the VC10 (UK Laboratory) Smoking Robot was done.

Comments (0) Number of views (399)

Categories: Publications

Tags: inhalation exposure, tobacco smoke, e-cigarettes, VC 10 smoking robots, VITROCELL 6, VITROCELL 12, VC 1 smoking machine, air-liquid Interface

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Gordon Research Conference, Integration of Emerging Technologies in Mechanistic and Translational Toxicology,Andover, August 11–16, 2019

09 March 2019, Hilton Inner Harbour Hotel, Baltimore, USA

Society of Toxicology, Annual Meeting 2019, Baltimore, MD, USA | 10-14 March

Manufacturers invest in nonanimal tests to evaluate health risks of e-cigarettes and other next-generation tobacco products

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