Advanced in vitro exposure systems.

3. Nov. 2022

Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module

https://doi.org/10.1016/j.toxrep.2022.11.001

Brian M. Keyser1, Robert Leverette1, Michael Hollings2, Adam Seymour2, Randy A. Weidman3, Carlton J. Bequette3, Kristen Jordan1
1 RAI Services Company; Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA
2 Labcorp Early Development Laboratories Ltd., Harrogate, North Yorkshire, UK
3 RJ Reynolds Tobacco Company; 950 Reynolds Blvd., Winston-Salem, NC 27106, USA

 

Highlights

  • Characterization of two 48 well exposure modules with three different product types
  • Comparison of these exposure modules using four different dosimetry techniques
  • Ability to differentiate the aerosol deliveries between different dilution airflows

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6. Apr. 2022

Product News 04-2/2022

VITROCELL® Smoking Machine VC 1 2.0

Manual smoking machine with high tech features

The VC 1 smoking machine is used for a controlled aerosol generation of combustion cigarettes as well as of next generation devices such as Heated Tobacco Products (HTP) and electronic nicotine delivery systems (ENDS). Special attention was given to guarantee very flexible programming of the smoking parameters. The properties of the aerosol are maintained due to small dead volumes of the system.

VITROCELL® Smoking Machine VC 1 2.0  (PDF)

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27. Mar. 2022

Nrf2 Responses of Commercial Cigarette Whole Smoke and Aerosol from Six Electronic Nicotine Delivery Systems (ENDS) in a 3D Human Airway Model

Poster SOT 2022, 61st Annual Meeting and ToxExpo, March 27-31, San Diego, USA

Brian M. Keyser1, John Wertman1, Michael Hollings2, Robert Bedford2, and Kristen Jordan1
1 Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC; 
2 Labcorp Early Development Laboratories Ltd., Harrogate, UK

In this study whole smoke from a marketed combustible cigarette and whole aerosol from four different ENDS were evaluated on cell viability and Nrf2 response in a 3D human airway model transfected with a luciferase Nrf2 promoter.
Whole aerosol was generated using a Vitrocell ® VC10® Smoke Exposure System. The tissues were exposed to whole smoke from a market combustible generated under Health Canada Intense regime.These data show that the 3D Nrf2 EpiAirway™ in vitro model can be used to assess and discriminate responses from a biomarker (oxidative stress) for disease pathways associated with tobacco product usage.

 

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27. Mar. 2022

Cytotoxicity Assessment of Electronic Nicotine Delivery Systems (ENDS) and Combustible Cigarette Aerosols Utilizing Standard and Whole Aerosol Exposure Approaches in the Neutral Red Uptake Assay

Poster SOT 2022, 61st Annual Meeting and ToxExpo, March 27-31, San Diego, USA

Robert Leverette1, Thomas Shutsky1, John Wertman1, Katarina Aleksa2, Dhatri Lakshmanan2, Rebecca Payne3, Kristen Jordan1
1Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC, USA; 
2Labstat International Inc. Kitchener, ON, Canada;
3Labcorp Early Development Laboratories Ltd., Harrogate, UK

This poster shows whole aerosol exposures of combustible and next generation tobacco products, including Electronic Nicotine Delivery Systems. A Vitrocell® VC10® robot generated and delivered aerosols to the Mammalian 6/48 aerosol dilution and exposure system, with up to 7 concurrent doses plus a clean air control. Dosimetry module allowed the capture and quantification of deposited aerosol constituents (nicotine, glycerol and carbonyls). The study utilized the Neutral Red Uptake (NRU) assay in which mammalian cells were exposed to either combined TPM + GVP (submerged culture) or Whole Aerosol (ALI).

 

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27. Mar. 2022

In Vitro Cytotoxicity Assessment of 3D Human Airway Tissue Following Exposure to Whole Aerosol/Smoke Generated from Electronic and Combustible Cigarettes

Poster SOT 2022, 61st Annual Meeting and ToxExpo, March 27-31, San Diego, USA

Thomas Shutsky1, Brian M. Keyser1, Kristen Jordan1, Michael Hollings2, and Emma Rothwell2
1Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC; 
2Labcorp Early Development Laboratories Ltd., Harrogate, UK

In this study, EpiAirway™ tissues were exposed to whole aerosol/smoke generated from six different Vuse Alto electronic nicotine delivery system test articles with various nicotine concentrations and a combustible cigarette. A Vitrocell® VC10® robot was used to generate whole aerosol/smoke with either a modified ISO or Health Canada Intense smoking regimen. Whole smoke/aerosol was puffed with clean air with a series of different air flows to achieve the delivered dose range. 

 

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22. Mar. 2022

Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium

https://doi.org/10.1172/jci.insight.155589

Kristine M. Abo,1,2 Julio Sainz de Aja,3,4,5 Jonathan Lindstrom-Vautrin,1 Konstantinos-Dionysios Alysandratos,1,2 Alexsia Richards,6 Carolina Garcia-de-Alba,3,4,5 Jessie Huang,1,2 Olivia T. Hix,1,2 Rhiannon B. Werder,1,2 Esther Bullitt,7 Anne Hinds,2 Isaac Falconer,8 Carlos Villacorta-Martin,1 Rudolf Jaenisch,6,9 Carla F. Kim,3,4,5 Darrell N. Kotton,1,2 and Andrew A. Wilson1,2
1Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, Massachusetts, USA. 
2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA.
3Stem Cell Program and Divisions of Hematology/Oncology and Pulmonary & Respiratory Diseases, Boston Children’s Hospital, Boston, Massachusetts, USA. 
4Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. 
5Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. 
6Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA. 
7Department of Physiology & Biophysics, Boston University, Boston, Massachusetts, USA. 
8Boston University School of Medicine, Boston, Massachusetts, USA. 
9Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

This study comprehensively characterize the effects of ALI culture on iAT2s and benchmark their transcriptional profile relative to both freshly sorted and cultured primary human fetal and adult AT2s. They find that iAT2s cultured at ALI maintain an AT2 phenotype while upregulating expression of transcripts associated with AT2 maturation. Then they leverage this platform to assay the effects of exposure to clinically significant, inhaled toxicants including cigarette smoke and electronic cigarette vapor.

 

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18. Mar. 2022

Fit-for-purpose characterization of air-liquid-interface (ALI) in vitro exposure systems for e-vapor aerosol

https://doi.org/10.1016/j.tiv.2022.105352

J. Zhang1, U. Doshi1, R.L. Wolz2, P. Kosachevsky2, M.J. Oldham1,2, I.G. Gillman2,2 , K.M. Lee1
1Altria Client Services LLC, Richmond, VA
2Enthalpy Analytical, Richmond, VA

Highlights:

  • We characterized an VITROCELL Ames 48 and a VC 24/48 for e-vapor aerosol toxicity evaluation.
  • The Ames 48 delivered 0.8 – 3.4 mg aerosol per insert under testing conditions.
  • The VC 24/48 delivered 1.0 – 6.4 mg aerosol per insert under testing conditions.
  • Delivered nicotine ranged up to approximately 200 μg per insert in both exposure systems.
  • The PSD as measured by cascade impactor were comparable prior to and after VC 24/48 or Ames 48.

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7. Feb. 2022

Pulmonary Delivery of Aerosolized Chloroquine and Hydroxychloroquine to Treat COVID-19: In Vitro Experimentation to Human Dosing Predictions

Aditya R. Kolli, 1 Tanja Zivkovic Semren, 1 David Bovard, 1 Shoaib Majeed, 1 Marco van der Toorn, 1 Sophie Scheuner, 1 Philippe A. Guy, 1 Arkadiusz Kuczaj, 1 Anatoly Mazurov, 1 Stefan Frentzel, 1 Florian Calvino-Martin, 1 Nikolai V. Ivanov, 1 John O’Mullane, 1 Manuel C. Peitsch, 1 and Julia Hoeng 1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000, Neuchâtel, Switzerland

This study shows formulated CQ and HCQ aerosols with respirable particles for use in the treatment of COVID-19. An in vitro assessment in human bronchial epithelial cells showed no adverse effects on cell viability, TEER, or ciliary beating. Modeling CQ and HCQ exposures in vitro in the Vitrocell 24 exposure system and in an IPML model enabled the validation of transport kinetics across the airway epithelial barrier.

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2. Feb. 2022

Smoking Machine VC 10 S-TYPE for Heat-not-Burn Tobacco Devices

Now Heated Tobacco Products (HTP) can be continuously smoked with automatic button actuation. The heat-and-turn feature for heat-not-burn devices enables automatic smoking at all 10 ports of the VC 10 S-TYPE Robot. Selective guidance of the aerosol to max. 6 exhaust lines is possible as well.

 

VITROCELL Application Note

Watch the video on Youtube

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3. Mar. 2021

Vitrocell® Leak Tester

Must-have device for aerosol research – easy and efficient leak detection

Regular leak tests are mandatory in aerosol research. A leak caused by a forgotten connection or defective o-ring may have a significant influence on the aerosol exposure process. We recommend to carry out a leak test prior to the experiment and as part of cleaning or service routine.

VITROCELL Application Note

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