DOI 10.1186/s12989-017-0186-4

Sebastian G. Klein^1,2, Sébastien Cambier¹, Jennifer Hennen², Sylvain Legay¹, Tommaso Serchi¹, Inge Nelissen³, Aline Chary¹, Elisa Moschini¹, Andreas Krein¹, Brunhilde Blömeke² and Arno C. Gutleb<sup>1

1</sup>Luxembourg Institute of Science and Technology (LIST), Environmental Research and Innovation (ERIN) Department, 41, rue du Brill, L-4422 Belvaux, Grand Duchy of Luxembourg
²Department of Environmental Toxicology, University Trier, Universitätsring 15, 54296 Trier, Germany

In this study tetracultures of alveolar epithelial cells (A549) were exposed to different doses of DEPM (SRM2975) at the air-liquid-interface to present the first approach to combine a complex 3D tetraculture in vitro system with native aerosol exposure equipment to mimic the exposure situation in vivo as closely as possible.

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DOI:10.1371/journal.pone.0157964

Sean C. Sapcariu^1,12, Tamara Kanashova^2,12, Marco Dilger^3,4,12, Silvia Diabaté^3,12, Sebastian Oeder^5,6,12,13, Johannes Passig^7,12, Christian Radischat^7,12, Jeroen Buters^5,6,12,13, Olli Sippula^8,12, Thorsten Streibel^7,9,12, Hanns-Rudolf Paur^4,12, Christoph Schlager^4,12, Sonja Mülhopt^4,12, Benjamin Stengel^10,12, Rom Rabe^10,12, Horst Harndorf^10,12, Tobias Krebs^11,12, Erwin Karg⁹, Thomas Gröger⁹, Carsten Weiss^3,12, Gunnar Dittmar^2,12, Karsten Hiller^1,12, Ralf Zimmermann^7,9,12

¹ Luxembourg Centre for Systems Biomedicine 6, avenue du Swing, L-4362 Esch-sur-Alzette, Luxembourg,
² Mass Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine Berlin-Buch, Berlin, Germany,
³ Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
⁴ Institute for Technical Chemistry (ITC), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
⁵ Center of Allergy and Environment (ZAUM), Helmholtz Zentrum München and Technische Universität München, Munich, Germany,
⁶ CK-CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland,
⁷ Joint Mass Spectrometry Centre, Division of Analytical and Technical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany,
⁸ University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland,
⁹ Joint Mass Spectrometry Centre, CMA – Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany,
¹⁰ Chair of Piston Machines and Internal Combustion Engines, University Rostock, Rostock, Germany,
¹¹ Vitrocell GmbH, Waldkirch, Germany,
¹² HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany; Kuopio, Finland; Cardiff, United Kingdom; Esch-Belval, Luxembourg,
¹³ German Center for Lung Research (DZL), Munich, Germany

An automated ALI exposure system station with 18 exposure positions was used as the interface for mouse macrophage RAW 264.7 cell line exposures of the diesel engine exhaust. The evaluation is devided in LDH release assay, Metabolite extraction and GC-MS processing, Stable isotope labeling by amino acids in cell culture (SILAC), Proteome extraction and LC-MS/MS analysis of peptides and Proteomics Data An

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February 5-6, 2013

Authors
Rebecca Greenan¹, Tak Chan², Mathias Könczöl³, Julie Bourdon¹, Debbie Rosenblatt², Marie-Josée Poitras²and Paul A. White¹

¹ Mechanistic Studies Division, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada
² Emissions Research and Measurement Section, Environment Canada, Ottawa, ON, Canada
³ Department of Pharmaceutical Biology and Biotechnology, University of Freiburg, Germany

Exposition of emissions from different diesel blends on A549 cells with endpoints for genotoxicity (WST-1, NRU, Caspase III/VII) and oxidative stress (TBARS).

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Volume 16, Issue 2, pp. 185-192, April, 2002

Authors
J.W. Knebel, D. Ritter, M. Aufderheide

The experimental set-up allows the investigation of native diesel exhaust using short distances for the transportation of the gas to the target cells. Cell viability was not influenced by the exposure to clean air, whereas dose-dependent cytotoxicity was found contingent on the dosage of exhaust.

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