Advanced in vitro exposure systems.

6. Mar. 2017

Endothelial responses of the alveolar barrier in vitro in a dose-controlled exposure to diesel exhaust particulate matter

DOI 10.1186/s12989-017-0186-4

Sebastian G. Klein1,2, Sébastien Cambier1, Jennifer Hennen2, Sylvain Legay1, Tommaso Serchi1, Inge Nelissen3, Aline Chary1, Elisa Moschini1, Andreas Krein1, Brunhilde Blömeke2 and Arno C. Gutleb1

1Luxembourg Institute of Science and Technology (LIST), Environmental Research and Innovation (ERIN) Department, 41, rue du Brill, L-4422 Belvaux, Grand Duchy of Luxembourg
2Department of Environmental Toxicology, University Trier, Universitätsring 15, 54296 Trier, Germany


In this study tetracultures of alveolar epithelial cells (A549) were exposed to different doses of DEPM (SRM2975) at the air-liquid-interface to present the first approach to combine a complex 3D tetraculture in vitro system with native aerosol exposure equipment to mimic the exposure situation in vivo as closely as possible.

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16. Dec. 2016

'Clean-burning' Fuels May be Worse for Your Lungs

Nala Rogers for 'Inside Science'

A report about artificial lung reveals surprising dangers from pellet burners and diesel-fueled ships at the University of Rostock and Helmholtz Zentrum München in Germany.

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27. Jun. 2016

Metabolic Profiling as Well as Stable Isotope Assisted Metabolic and Proteomic Analysis of RAW 264.7 Macrophages Exposed to Ship Engine Aerosol Emissions

Different Effects of Heavy Fuel Oil and Refined Diesel Fuel

DOI:10.1371/journal.pone.0157964

Sean C. Sapcariu1,12, Tamara Kanashova2,12, Marco Dilger3,4,12, Silvia Diabaté3,12, Sebastian Oeder5,6,12,13, Johannes Passig7,12, Christian Radischat7,12, Jeroen Buters5,6,12,13, Olli Sippula8,12, Thorsten Streibel7,9,12, Hanns-Rudolf Paur4,12, Christoph Schlager4,12, Sonja Mülhopt4,12, Benjamin Stengel10,12, Rom Rabe10,12, Horst Harndorf10,12, Tobias Krebs11,12, Erwin Karg9, Thomas Gröger9, Carsten Weiss3,12, Gunnar Dittmar2,12, Karsten Hiller1,12, Ralf Zimmermann7,9,12

1 Luxembourg Centre for Systems Biomedicine 6, avenue du Swing, L-4362 Esch-sur-Alzette, Luxembourg,
2 Mass Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine Berlin-Buch, Berlin, Germany,
3 Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
4 Institute for Technical Chemistry (ITC), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
5 Center of Allergy and Environment (ZAUM), Helmholtz Zentrum München and Technische Universität München, Munich, Germany,
6 CK-CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland,
7 Joint Mass Spectrometry Centre, Division of Analytical and Technical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany,
8 University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland,
9 Joint Mass Spectrometry Centre, CMA – Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany,
10 Chair of Piston Machines and Internal Combustion Engines, University Rostock, Rostock, Germany,
11 Vitrocell GmbH, Waldkirch, Germany,
12 HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany; Kuopio, Finland; Cardiff, United Kingdom; Esch-Belval, Luxembourg,
13 German Center for Lung Research (DZL), Munich, Germany

An automated ALI exposure system station with 18 exposure positions was used as the interface for mouse macrophage RAW 264.7 cell line exposures of the diesel engine exhaust. The evaluation is devided in LDH release assay, Metabolite extraction and GC-MS processing, Stable isotope labeling by amino acids in cell culture (SILAC), Proteome extraction and LC-MS/MS analysis of peptides and Proteomics Data An

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3. Jun. 2015

Particulate Matter from Both Heavy Fuel Oil and Diesel Fuel Shipping Emissions Show Strong Biological Effects on Human Lung Cells at Realistic and Comparable In Vitro Exposure Conditions

DOI: 10.1371/journal.pone.0126536

Authors

Sebastian Oeder , Tamara Kanashova , Olli Sippula , Sean C. Sapcariu , Thorsten Streibel , Jose Manuel Arteaga-Salas , Johannes Passig , Marco Dilger, Hanns-Rudolf Paur, Christoph Schlager, Sonja Mülhopt, Silvia Diabaté, Carsten Weiss, Benjamin Stengel, Rom Rabe, Horst Harndorf, Tiina Torvela, Jorma K. Jokiniemi, Maija-Riitta Hirvonen, Carsten Schmidt-Weber, Claudia Traidl-Hoffmann, Kelly A. BéruBé, Anna J. Wlodarczyk, Zoë Prytherch, Bernhard Michalke, Tobias Krebs, André S. H. Prévôt, Michael Kelbg, Josef Tiggesbäumker, Erwin Karg, Gert Jakobi, Sorana Scholtes, Jürgen Schnelle-Kreis, Jutta Lintelmann, Georg Matuschek, Martin Sklorz, Sophie Klingbeil, Jürgen Orasche, Patrick Richthammer, Laarnie Müller, Michael Elsasser, Ahmed Reda, Thomas Gröger, Benedikt Weggler, Theo Schwemer, Hendryk Czech, Christopher P. Rüger, Gülcin Abbaszade, Christian Radischat, Karsten Hiller, Jeroen T. M. Buters , Gunnar Dittmar , Ralf Zimmermann
Institutes

 

Exposition of Heavy Fuel Oil and Diesel Fuel Shipping Emissions on human lung cells in the air-liquid interface exposure system for advanced chemical analysis combined with transcriptional, proteomic and metabolomic profiling and isotope labelling methods.

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6. Feb. 2013

Optimization of the VITROCELL® Exposure System for in vitro toxicity testing of diesel emissions at the air-liquid interface

Transportation Technologies and Fuels Forum (TTFF) in Ottawa, Ontario, Canada

February 5-6, 2013

Authors
Rebecca Greenan1, Tak Chan2, Mathias Könczöl3, Julie Bourdon1, Debbie Rosenblatt2, Marie-Josée Poitras2and Paul A. White1

1 Mechanistic Studies Division, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada
2 Emissions Research and Measurement Section, Environment Canada, Ottawa, ON, Canada
3 Department of Pharmaceutical Biology and Biotechnology, University of Freiburg, Germany

 

Exposition of emissions from different diesel blends on A549 cells with endpoints for genotoxicity (WST-1, NRU, Caspase III/VII) and oxidative stress (TBARS).

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23. Mar. 2007

Responses of differentiated primary human lung epithelial cells to exposure to diesel exhaust at an air-liquid interface

Experimental Lung Research

33:1, 27 - 51, 2007

Authors
JeanClare Seagrave, Sandy Dunaway, Jacob D. McDonald, and Joe L. Mauderly
Lovelace Respiratory Research Institute, Albuquerque, New Mexico, USA

Patrick Hayden
MatTek Corp, Ashland, Massachusetts, USA

Christine Stidley
Department of Internal Medicine, University of New Mexico, Albuquerque, New Mexico, USA

 

The study evaluated responses of human primary airway epithelial cells to diesel exhaust. This model may be useful for mechanistic and comparative toxicology studies.

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30. Apr. 2002

Exposure of human lung cells to native diesel motor exhaust-development of an optimized in vitro test strategy

Toxicology in Vitro

Volume 16, Issue 2, pp. 185-192, April, 2002

Authors
J.W. Knebel, D. Ritter, M. Aufderheide

 

The experimental set-up allows the investigation of native diesel exhaust using short distances for the transportation of the gas to the target cells. Cell viability was not influenced by the exposure to clean air, whereas dose-dependent cytotoxicity was found contingent on the dosage of exhaust.

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31. Mar. 2002

Responses of cultured lung epithelial cells to airborne diesel emissions

Diesel Engine Emissions Reduction Workshop

Paper issued March, 2002

Authors
J. C. Seagrave, J. D. McDonald, and J. L. Mauderly

Lovelace Respiratory Research Institute


Investigation of diesel exhaust on human lung cells. Results were evaluated by measurement of ATP, WST-1, LDH release, IL-8, Glutathione and alkaline phosphatase.

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