Joana Candeiasa, Elias J. Zimmermannbc, Christoph Bisigb, Nadine Gawlittabc, Sebastian Oederb, Thomas Grögerb, Ralf Zimmermannbc, Carsten B. Schmidt-Webera, Jeroen Butersa
a Center Allergy & Environment (ZAUM), Member of the German Center for Lung Research (DZL), Technical University Munich / Helmholtz Center Munich, Germany
b Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics (CMA), Helmholtz Center Munich, Ingolstädter Landstraße 1, D-85764, Neuherberg, Germany
c Joint Mass Spectrometry Center (JMSC) at Analytical Chemistry, Institute of Chemistry, University of Rostock, Dr. Lorenzweg 2, D-18051, Rostock, Germany
Human bronchial epithelial BEAS-2B cells were exposed to native birch pollen (real life intact pollen, not pollen extracts) at the air-liquid interface (pollen-ALI). BEAS-2B cells were also pre-exposed in a diesel-ALI to diesel CAST for 2 h (a model for diesel exhaust) and then to pollen in the pollen-ALI 24 h later. Effects were analysed by genome wide transcriptome analysis after 2 h 25 min, 6 h 50 min and 24 h. Selected genes were confirmed by qRT-PCR.