Advanced in vitro exposure systems.

18. Sep. 2020

VITROCELL® Shisha Testing System for Cell Cultures

For in vitro testing of water pipe tobacco at the air / liquid interface

New designs of electronic cigarettes such as ENDS (Electronic Nicotine Delivery Systems) products or HTP (Heated Tobacco Products) lead to a large variety of different shapes which make the insertion into conventional holders with labyrinth seals impossible. VITROCELL® has developed a new holder system which is flexible to adjust to different shapes.

VITROCELL Application Note

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24. Aug. 2020

Product News 08/2020

VITROCELL® Cloud Alpha 96

96-well Air/Liquid Interface (ALI) exposure. For reliable high throughput testing of cell cultures.

The VITROCELL® Cloud Alpha 96 is our newest innovation and presents a great leap forward in automated exposure of cell cultures. It combines high throughput testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12- and 24-well). It’s functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the air/liquid interface have never been easier.

VITROCELL® Cloud Alpha 96

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27. Jul. 2020

Vitrocell® holder systems for e-cigarettes

Secure and tight connection of any device to the smoking machine.

New designs of electronic cigarettes such as ENDS (Electronic Nicotine Delivery Systems) products or HTP (Heated Tobacco Products) lead to a large variety of different shapes which make the insertion into conventional holders with labyrinth seals impossible. VITROCELL® has developed a new holder system which is flexible to adjust to different shapes.

VITROCELL Application Note

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26. Jul. 2020

Comparative toxicity of ultrafine particles around a major airport in human bronchial epithelial (Calu-3) cell model at the air–liquid interface

https://doi.org/10.1016/j.tiv.2020.104950

Authors

Rui-WenHeab, Miriam E.Gerlofs-Nijlanda, JohnBoerea, PaulFokkensa, DaanLesemana, Nicole A.H.Janssena, Flemming R.Casseeab
a National Institute for Public Health and the Environment (RIVM), P.O. Box, 3720, BA, Bilthoven, the Netherlands
b Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80178, 3508, TD, Utrecht, the Netherland

 

Highlights

  • Airport and road traffic UFPs can activate inflammation in Calu-3 cells.
  • Airport UFPs exert similar toxicity compared to UFPs from road traffic emission.
  • ALI condition promotes cellular responses to particles at low exposed dose.

 

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25. Jun. 2020

Novel TEM Grid holders for dose determination of airborne particles during in vitro Exposure at the air / liquid interface

The assessment of toxicological effects of airborne particles to the human organism is of major importance in disease research. The presence of nano and ultrafine particles can be found both in indoor and outdoor atmospheres which include workplaces and living areas. Over the last years, the degree of contribution to diseases of such particles is analyzed ever more frequent by the use of in vitro methods. The required equipment for these studies consisting of aerosol generation and exposure systems are nowadays developed more and more to proven levels.

VITROCELL Application Note.

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21. May. 2020

A novel TEM grid sampler for airborne particles to measure the cell culture surface dose

Sonja Mülhopt1, Christoph Schlager2, Markus Berger2, Sivakumar Murugadoss3, Peter H. Hoet3, Tobias Krebs2, Hanns-Rudolf paur1 & Dieter Stapf1
1Karlsruhe Institute of Technology (KIT), Institute for Technical Chemistry, Eggenstein-Leopoldshafen, 76344, Germany. 
2Vitrocell Systems GmbH, Waldkirch, 79183, Germany. 
3KU Leuven, Environment and Health, Leuven, 3000, Belgium.

 

The surface dose and the spatial distribution on the membrane delivers important data for  measuring dose-response relationships in toxicity studies.  Image evaluation of transmission electron  microscopy (TEM) samples is a highly sensitive method for determination of deposition. This paper reports  the development and characterization of a novel holder for film coated TEM copper grids, which allows for  sampling under identical geometric and ambient conditions as in a cell culture chamber. 

 

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13. May. 2020

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

DOI:10.3791/61210 


Hedwig M. Braakhuis1, Ruiwen He1,2, Rob J. Vandebriel1, Eric R. Gremmer1, Edwin Zwart1, Jolanda P. Vermeulen1, Paul Fokkens1, John Boere1, Ilse Gosens1, Flemming R. Cassee1,2 
1National Institute for Public Health and the Environment (RIVM) 
2Institute for Risk Assessment Sciences (IRAS) 


This article provides a method for culturing and exposing the human bronchial epithelial cell line Calu-37 at the Air-liquid Interface that mimics realistic, repeated inhalation exposure conditions that can be used for toxicity testing. By applying a continuous airflow using the Automated Exposure System, the cell model can be exposed to a low concentration of particles over a longer time period, reflecting realistic exposure conditions. Characteristics of both the cell model and of  the exposure system are essential for achieving a realistic inhalation exposure model that can be used for repeated exposures. 

 

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10. Mar. 2019

New Products at VITROCELL® User Group Meeting 2019

09 March 2019, Hilton Inner Harbour Hotel, Baltimore, USA

Following the VITROCELL® User Group Meeting 2018 in San Antonio, Texas, USA, international scientists from Europe, Japan, Korea and USA reconvened in March 2019.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers.
The meeting took place prior to the international Society of Toxicology 58th Annual Meeting and ToxExpo, March 10 – 14, 2018, in Baltimore, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2019.

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28. Oct. 2018

Big tobacco embraces in vitro toxicology

Manufacturers invest in nonanimal tests to evaluate health risks of e-cigarettes and other next-generation tobacco products

c&en, Volume 96, Issue 43

Britt E. Erickson

The complexity of next-generation tobacco products has increased by a number of factors since FDA was given responsibility to regulate tobacco. Testing e-liquids with animals is not practical, economically and not that accurate. The Vitrocell system generates airborne materials from test products, such as smoke from reference cigarettes and vapor from e-cigarettes, in a manner that simulates how a person would puff. This artcile shows, how IIVS scientists use human donor tissues, obtained from organs, to create cell cultures and in vitro test systems.


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6. Jun. 2018

Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

DOI: 10.2217/nnm-2017-0358

Savvina Chortarea1,2, Kleanthis Fytianos1,3,4, Laura Rodriguez-Lorenzo1, Alke Petri-Fink1,5 & Barbara Rothen-Rutishauser1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
2Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
3Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
4Department of Clinical Research, University of Bern, Bern, Switzerland
5Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

 

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