Advanced in vitro exposure systems.

31. Dec. 2006

Dynamic in-vitro Exposure of Human Derived Cells to Indoor Priority Pollutants


Report EUR 22285 EN, 2006

F. Pariselli, M. G. Sacco, D. Rembges

Institute for Health and Consumer Protection, Physical and Chemical Exposure Unit, Ispra, Italy


The aim of this work was to investigate toxicity of single volatile chemicals and their mixtures. Developing a reproducible in vitro dynamic exposure model where A549 and HaCaT cell lines representative of VOC’s target tissues (lungs and skin) were exposed in an airlifted interface to air pollutants. Several endpoints were evaluated: cytotoxicity (LDH), proliferation (WST-1), CFU (Colony Forming Units), inflammatory response (IL-8) and oxidative stress (ROS).

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31. Oct. 2005

Exposure System for Bioassays Measurement of the Toxicity of Airborne Nanoparticles

Research Center Karlsruhe

Dr. Paur, S. Mülhopt and Dr. Diabate


The exposure of human lung cells towards nano particles the correlation between particle diameter, material properties, number concentration and biological response could be determined.

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25. Apr. 2005

Experimental Assessment of the Toxicological Effects of Inhaled Complex Mixtures on the Respiratory System - Feasibility and Limitations


Exp Toxic Pathol 2005, Supplement issued July, 2005

Workshop 23 - 25 April, 2005 in Barcelona, Spain

In this Workshop data were shown that demonstrate the response of cultured non-tumorigenic human bronchial epithelial cells (BEAS-2B) exposed to air pollution particles that were similar to the response seen in humans following exposure to the same particles. A presentation of the work with the air-liquid interface were given to compare in-vitro and in-vivo methods.

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31. Dec. 2004

Comparative Assessment of Toxicities of Mainstream Smoke from Commercial Cigarettes

Taylor & Francis

Inhalation Toxicology 16:1-10, 2004

Detlef Ritter, Jan W. Knebel, and Michaela Aufderheide


Online monitoring of generated smoke aerosols with respect to particulate and gas-phase components, and direct exposure of A549 cell line using exposure conditions relevant to human smoking.Results are evaluated in measuring glutathione and ED50.

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31. Dec. 2003

Novel approaches for studying pulmonary toxicity on vitro

Toxicology Letters

ELSEVIER, 140-141 (2003) 205-211

Michaela Aufderheide, Jan W. Knebel, Detlef Ritter


The goal of this studiy is to analyze the biological effects of test atmospheres under environmental conditions without humidifying the atmosphere or adding additional CO2.

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30. Mar. 2003

Exposure of human lung cells to inhalable substances

Taylor & Francis

Inhalation Toxicology, 15:67-84, 2003

Detlef Ritter, Jan W. Knebel, Michaela Aufderheide


The experimental setup included online monitoring of the gas phase and particulate phase. A cell exposure of different smoke and synthetic air periods was sufficient to cause different effects as measured by intracellular glutathione content. The fact that the system could differentiate between different types of cigarette smoke demonstrated its high sensitivity and specificity. 

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25. Feb. 2003

An improved in vitro model for testing the pulmonary toxicity of complex mixtures such as cigarette smoke


Exp Toxic Pathol 2003; 55: 51-57

Michaela Aufderheide, Jan W. Knebel, and Detlef Ritter


The study presented a system that enables the direct exposure of human lung cells to native, unmodified cigarette mainstream smoke and also the demonstration of reproducible particle deposition depending on smoke concentration. Furthermore, it was shown that the exposed cells maintained their viability. The results were shown in measuring the cellular viability, ATP and glutathione content. 

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31. Dec. 2002

A Method for the In Vitro Exposure of Human Cells to Environmental and Complex Gaseous Mixtures: Application to Various Types of Atmosphere


Issue 30, pp. 433-441, 2002

Michaela Aufderheide, Jan W. Knebel and Detlef Ritter


This study presents the testing of various generated atmospheres under the same cell-exposure conditions and demonstartion of studying dose-dependent cytotoxic effects in human lung epithelial cells exposed to air contaminated with single gases or complex mixtures, such as diesel exhaust fumes and side-stream cigarette smoke.

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30. Jun. 2002

In vitro genotoxicity assay of sidestream smoke using a human bronchial epithelial cell line

Food and Chemical Toxicology

Volume 40, Issue 6, pp. 845-850, June, 2002

L. Wolz, G. Krause, G. Scherer, M. Aufderheide, U. Mohr


In this study, genotoxic effects of sidestream cigarette smoke at different concentrations were assessed using the alkaline comet assay. HFBE 21 cells were exposed to clean air, nitrogen dioxide or sidestream smoke. 

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30. Apr. 2002

Exposure of human lung cells to native diesel motor exhaust-development of an optimized in vitro test strategy

Toxicology in Vitro

Volume 16, Issue 2, pp. 185-192, April, 2002

J.W. Knebel, D. Ritter, M. Aufderheide


The experimental set-up allows the investigation of native diesel exhaust using short distances for the transportation of the gas to the target cells. Cell viability was not influenced by the exposure to clean air, whereas dose-dependent cytotoxicity was found contingent on the dosage of exhaust.

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