Advanced in vitro exposure systems.

25. Feb. 2003

An improved in vitro model for testing the pulmonary toxicity of complex mixtures such as cigarette smoke

URBAN & FISCHER

Exp Toxic Pathol 2003; 55: 51-57

Authors
Michaela Aufderheide, Jan W. Knebel, and Detlef Ritter

 

The study presented a system that enables the direct exposure of human lung cells to native, unmodified cigarette mainstream smoke and also the demonstration of reproducible particle deposition depending on smoke concentration. Furthermore, it was shown that the exposed cells maintained their viability. The results were shown in measuring the cellular viability, ATP and glutathione content. 

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31. Dec. 2002

A Method for the In Vitro Exposure of Human Cells to Environmental and Complex Gaseous Mixtures: Application to Various Types of Atmosphere

ATLA

Issue 30, pp. 433-441, 2002

Authors
Michaela Aufderheide, Jan W. Knebel and Detlef Ritter

 

This study presents the testing of various generated atmospheres under the same cell-exposure conditions and demonstartion of studying dose-dependent cytotoxic effects in human lung epithelial cells exposed to air contaminated with single gases or complex mixtures, such as diesel exhaust fumes and side-stream cigarette smoke.

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30. Jun. 2002

In vitro genotoxicity assay of sidestream smoke using a human bronchial epithelial cell line

Food and Chemical Toxicology

Volume 40, Issue 6, pp. 845-850, June, 2002

Authors
L. Wolz, G. Krause, G. Scherer, M. Aufderheide, U. Mohr

 

In this study, genotoxic effects of sidestream cigarette smoke at different concentrations were assessed using the alkaline comet assay. HFBE 21 cells were exposed to clean air, nitrogen dioxide or sidestream smoke. 

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30. Apr. 2002

Exposure of human lung cells to native diesel motor exhaust-development of an optimized in vitro test strategy

Toxicology in Vitro

Volume 16, Issue 2, pp. 185-192, April, 2002

Authors
J.W. Knebel, D. Ritter, M. Aufderheide

 

The experimental set-up allows the investigation of native diesel exhaust using short distances for the transportation of the gas to the target cells. Cell viability was not influenced by the exposure to clean air, whereas dose-dependent cytotoxicity was found contingent on the dosage of exhaust.

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31. Mar. 2002

Responses of cultured lung epithelial cells to airborne diesel emissions

Diesel Engine Emissions Reduction Workshop

Paper issued March, 2002

Authors
J. C. Seagrave, J. D. McDonald, and J. L. Mauderly

Lovelace Respiratory Research Institute


Investigation of diesel exhaust on human lung cells. Results were evaluated by measurement of ATP, WST-1, LDH release, IL-8, Glutathione and alkaline phosphatase.

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Comments (0) Number of views (1292)
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