Advanced in vitro exposure systems.

11. Sep. 2009

The QCM - Online Dose Measurement with high relative Humidity

European Aerosol Conference. Karlsruhe, Germany.

September 06-11, 2009

Authors
S. Mülhopt, A. Comouth, A. Grotz, T. Krebs, H.-R. Paur

Institute for Technical Chemistry, Thermal Waste Treatment Division, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
Institute for Meteorology and Climate Research, Atmospheric Aerosol Research Department, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
VITROCELL Systems GmbH, Waldkirch, Germany

 

The shown method first time provides an online dose measurement for in vitro exposure experiments at the air-liquid interface also under high humidity conditions.  Human lung cell cultures were tested for different biological responses like the viability (LDH, AlamarBlue) as well as the release of IL-8.

Read more

Comments (0) Number of views (1492)

3. Sep. 2009

Air/Liquid Culture and Exposure Technique: Preliminary Results of a Prevalidation Study

World Congress on Alternatives and Animal Use in the Life Sciences. Rome, Italy.

August 30 - September 3, 2009

Authors
Smirnova, L.1, Liebsch, M.1, Tharmann, J.1, Pirow, R.1, Luch, A.1, Bauer, M.2, Graebsch, C.2, Linsel, G.3, Siemers, R.3, Berger-Preiß, E.4, Kock, H.4, Oertel, A.4, Riiter, D.4, Knebel, J.4

1 Federal Institute for Risk Assessment (BfR), Unit 37, Centre for Alternative Methods to Animal Experiments, ZEBET, Berlin, Germany
2 Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany
3 Federal Institute for Occupational Safety and Health (BAuA), Berlin, Germany
4 Fraunhofer Institute of Toxicology and Experimental Medicine (ITEM), Hannover, Germany

 

The aim of this study is to determine the in vitro vs. in vivo dose-response relationships. A549 cell were grwon in the air-liquid interface and exposed to NO2, SO2, formaldehyde and ozone. The results are measured in cytotoxicity (viability) and genotoxicity (COMET assay). The project has proved satisfying. transferability of the test method.

Read more

Comments (0) Number of views (1297)

23. Mar. 2009

Lung toxicity determination by in vitro exposure at the air-liquid interface with an integrated online dose measurement

Nanosafe 2008: International Conference on Safe production and use of nanomaterials

Journal of Physics: Conference Series 170 (2009) 012008 (doi:10.1088/1742-6596/170/1/012008)

Authors
Sonja Mülhopt, S. Diabaté, T. Krebs, C. Weiss and H.-R. Paur

Institute for Technical Chemistry, Thermal Waste Treatment Division, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
VITROCELL Systems GmbH, 79183, Waldkirch, Germany

 

Human lung cell cultures are exposed to airborne nanoparticles in VITROCELL®system modules. After exposure the cells are analyzed to measure the biological responses of the viability (LDH, AlamarBlue) as well as the release of IL-8. Using fluorescein sodium particles shows a very good correlation between the sensor signal of the quartz crystal microbalance and the deposited mass on the membranes shown by spectroscopy. This system for the first time provides an online dose measurement for in vitro experiments with nanoparticles. 

Read more

Comments (0) Number of views (1174)

19. Mar. 2009

Identification of Indoor Air Contaminants Using an in Vitro Exposure System

SOT Baltimore, USA

March 15-19, 2009

Authors
Laurel G. Jackson, J. R. Wells, Jason Ham, Albert Munson, and Stacey E. Anderson

National Institute for Occupational Safety and Health, (CDC-NIOSH), Morgantown, USA

 

A549 cells were exposed to volatile organic compounds in the air-liquid interface. The results are measured in RNA isolation and  gene expression analysis.

Read more

Comments (0) Number of views (1260)

8. Oct. 2008

Online dose determination for in vitro experiments with nano particles in the Karlsruhe Exposure System

EUROTOX. Rhodes, Greece.

October 5-8, 2008

Authors
S. Mülhopt, H.-R. Paur, T. Krebs

Forschungszentrum Karlsruhe, Eggenstein – Leopoldshafen, Germany
VITROCELL Systems GmbH, Waldkirch, Germany

 

Poster about the exposition of airborne nanoparticles to human lung cell cultures in VITROCELL®system modules. After exposure the cells are analyzed to measure the biological responses of the viability (LDH, AlamarBlue) as well as the release of IL-8. Using fluorescein sodium particles shows a very good correlation between the sensor signal of the quartz crystal microbalance and the deposited mass on the membranes shown by spectroscopy. This system for the first time provides an online dose measurement for in vitro experiments with nanoparticles. 

Read more

Comments (0) Number of views (1310)

10. Mar. 2008

The Response of a Co-culture Lung Model to Fine and Ultrafine Particles of Incinerator Fly Ash at the Air-liquid Interface

ATLA

ATLA, Issue 36, pp. 285-298, 2008

Authors
Silvia Diabaté, Sonja Mülhopt, Hanns-Rudolf Paur and Harald F. Krug

Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein - Leopoldshafen, Germany

 

BEAS-2B cells were exposed to an aerosol, generated from fly ash. The cells were co-cultured with differentiated THP-1 macrophages growing on Transwell inserts. Analyses of cell viability and IL-8 release were performed.

Read more

Comments (0) Number of views (1178)

10. Mar. 2008

Exposure of a multi-cellular in vitro model of human airway tissue to the whole cigarette smoke

Respiratory and Critical Care Medicine

Vol 177 (Abstracts Issue): pA728 (2008)

Authors
Walsh, P.T., Chalupowicz, D.G., Frankowski, R., Barnette, M.

Discovery Biology, Respiratory CEDD, GlaxoSmithKline, Upper Merion, PA19406, USA

 

HBE cells were exposed in a single and repeated whole smoke situation. The cells were analysed for changes in gene expression (RT-PCR) and cell viability was confirmed using the MTT assay and release of IL-1, IL-6 and IL-8.

Read more

Comments (0) Number of views (1187)

10. Mar. 2008

Cellular responses of primary human bronchial epithelial cells to whole cigarette smoke exposure

Respiratory and Critical Care Medicine

Vol 177 (Abstracts Issue): pA199 (2008)

Authors
Chalupowicz, D.G., Frankowski, R., Sanchez, Y., Kou, J., Barnette, M., and Walsh, P.T.

GlaxoSmithKline


Primary human bronchial epithelial cells were exposed in a single and repeated whole smoke situation. The cells were analysed for changes in gene expression (RT-PCR) and cell viability was confirmed using the MTT assay and release of IL-1, IL-6 and IL-8.

Read more

Comments (0) Number of views (1436)

31. Oct. 2007

In Vitro Testing of Inhalable Fly Ash at the Air Liquid Interface

Advanced Environmental Monitoring

October 2007, pp 402-414

Authors
Sonja Mülhopt, Hanns-Rudolf Paur, Silvia Diabaté and Harald F. Krug

Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein - Leopoldshafen, Germany

 

Exposure experiments with the fine and ultrafine fraction of fly ash on human lung cells in the air-liquid interface have shown an increased release of IL-8. 

Read more

Comments (0) Number of views (1291)

7. Oct. 2007

Toxicological investigation of nanoparticles - effects on human cells

EUROTOX. Amsterdam, Netherlands.

October 7-10, 2007

Authors
Letizia Farmer, Alexander Graff, Sandra Szameit, Eva Valic, Helga Tuschl

Austrian Research Centers GmbH - ARC, Life Sciences, Toxicology, A-2444 Seibersdorf, Austria

 

Differentiated macrophages were seeded on human lung epithelial cells for co-culturing. As endpoints for particle exposure cell viability (WST-1 assay), oxidative stress (DHRAssay) and pro-inflammatory cytokines (BDTM CBA-Assay) were evaluated.

Read more

Comments (0) Number of views (1407)
RSS
First9101112131415161718
Back to Top