Advanced in vitro exposure systems.

28. Sep. 2011

Aerosolized ZnO nanoparticles induce toxicity in alveolar type II epithelial cells at the air-liquid interface

Toxicol Sci

2012 Feb;125(2):450-61 (doi:10.1093/toxsci/kfr251) [Epub ahead of print]

Authors
Xie Y, Williams NG, Tolic A, Chrisler WB, Teeguarden JG, Maddux BL, Pounds JG, Laskin A, Orr G.

Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA

 

The exposure of alveolar type II epithelial cells (C10) to aerosolized nano particles at the ALI is shown in this article. Cell proliferation, cell viability, membrane integrity and oxidative stress were used as endpoints. 

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31. Aug. 2011

Effect of Formaldehyde On Corneal Epithelial Cells In An Air-Liquid Culture Model

EUROTOX Paris, France

August 28-31, 2011; Poster #P1198

Authors
Françoise Brignole-Baudouin1,2,4, Sophie Achard3, Charles Persoz3, Alice Emptoz1,2, Nathalie Seta3, Christophe Baudouin1,2,4

1 UMR_S968 INSERM UPMC; UMR 7210 CNRS; Institut de la Vision, Paris, France
2 Laboratoire de Toxicologie, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes
3 Laboratoire de Santé Publique et d’Environnement, EA 4064, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes
4 Centre Hospitalier National d’Ophtalmologie des Quinze-Vingts, 75012 Paris

 

The aim of this poster is to show the inflammatory effect of gaseous formaldehyde on human corneal epithelial cells in an air-liquid culture.The Results are shown in protein assay, LDH, IL-8, MCP-1 and flow cytometry assays.

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31. Aug. 2011

In vitro model of repeated exposure to different contaminants of human respiratory cells

EUROTOX Paris, France

August 28-31, 2011; Poster #P1361

Authors
Charles PERSOZ 1, Christopher LELEU 2, Sophie ACHARD 1, Magali FASSEU 3, Jean MENOTTI 2, Pascale MENECEUR 2, Francis DEROUIN 2, Nathalie SETA 1

1 Laboratoire Santé Publique et Environnement, EA4064,Université Paris Descartes, Paris, France
2 Laboratoire de Parasitologie-Mycologie, EA 3520, Université Paris Diderot, Paris, France
3 Centre de Recherche Biomédicale Bichat-Beaujon, Inserm, U773, Paris, France

 

The aim of this poster is to show the development of an in vitro model to exposure two different pollutants, a chemical pollutant (formaldehyde-FA) and a biological contaminant (Aspergillus fumigatus-Asp),were assessed in vitro on epithelial respiratory cells (A549).The results are shown in cellular viability (LDH assay), local inflammation, IL-8, MCP-1 and mRNA expression quantified by RT-PCR.

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31. Aug. 2011

Comparison of two in vitro models to assess the impact of environmentail pollutant exposures on respiratory cells

EUROTOX Paris, France

August 28-31, 2011; Poster #P1362

Authors
Charles Persoz 1, Christopher Leleu 2, Sophie Achard 1, Isabelle Momas 1, Nathalie Seta 1

1 Laboratoire de Santé Publique et Environnement, EA 4064. Université Paris Descartes, Paris, France
2 Laboratoire de Parasitologie-Mycologie, EA 3520, Université Paris-Diderot, Paris, France

 

The aim of this poster is to compare two in vitro approaches. Human alveolar epithelial cells (A549) were exposed at the Liquid-Liquid or Air-Liquid interface to various concentrations of Formaldehyde. The results are shown in IL-6, IL-8, MCP-1 and mitochondial activity assay.

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31. Aug. 2011

A novel dynamic gas mixing system to assess the human lung cells response to BTXE exposure at low levels

EUROTOX Paris, France

August 28-31, 2011; Poster #P2056

Authors
Sophie ACHARD 1, Mathilde DELAVAL 1, Tobias KREBS 2, Isabelle MOMAS 1, Nathalie SETA 1

1 Laboratory of Public Health and Environment, EA 4064, Paris Descartes University, F-75006 Paris
2 VITROCELL Systems GmbH, Waldkirch, Germany

 

To set up and validate the use of the Vitrocell® Spiking System (VSS), a novel gas mixing system generating low doses of VOCs, coupled to a system of exposure in air/liquid interface, to estimate the VOCs impact on the inflammatory response of airways cells.

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25. Aug. 2011

A novel approach to assess irritant or respiratory allergenic potential of chemicals in vitro

World Congress on Alternatives and Animal Use in the Life Sciences. Montréal, Canada.

August 21-25, 2011

Authors
Singal M.1, Vitale D.1, Smith L.1, Czollner K.2, Farmer L.2, Höpfner C.2, Satzer H-P.2, Schoeffmann S.2, Schwartz B.2, Weber E.2

1 Research Institute for Fragrance Materials, Inc., Woodcliff Lake, NJ, USA
2 Seibersdorf Labor GmbH, Seibersdorf, Austria

 

This article shows a method for the identification of chemical respiratory allergens. Two respiratory allergens were applied to a 3D co-culture cell system model at the air/liquid interface, mimicking the in-vivo situation in the lung. 
The results are shown in cytotoxicity (Propidium iodide, PI), Oxidative burst (Dihydrorhodamine assay, DHR), Pro- and antiinflammatory cytokines IL-2, IL-8, IL-10, IL-13, CD54, TNF-α, IFN-γ, MCP-1, RANTES (Cytometric Bead Array, CBA) and Staining of dendritic cell maturation marker CD86

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22. Aug. 2011

Prävalidierungsstudie zur Prüfung der toxischen Wirkung von inhalativ wirksamen Stoffen (Gase)

Prevalidation study for testing toxic effects of inhalable substances (gases)

1. Auflage. Dortmund: Bundesanstalt für Arbeitsschutz und Arbeitsmedizin 2011

Authors
G. Linsel, M. Bauer, E. Berger-Preiß, C. Gräbsch, H. Kock, M. Liebsch, R. Pirow, D. Ritter, L. Smirnova, J. Knebel: ISBN: 978-3-88261-131-1, 43 Seiten, Projektnummer: F 1835, Papier, PDF-Datei

 

The aim of the study was the prevalidation of an inhalation toxicity test for gases using human lung cells exposed on the air liquid interface. Four gases, nitrogen dioxide (NO2), sulfur dioxide (SO2), formaldehyde, ozone as well as synthetic air for negative control were investigated and evaluated in cytotoxicity and genotoxicity endpoints. (german article)

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12. Aug. 2011

Ex vivo toxicity of nitrogen dioxide in human nasal epithelium at the WHO defined 1-h limit value

Toxicology Letters

2011 Nov 10;207(1):89-95. doi: 10.1016/j.toxlet.2011.08.004. [Epub ahead of print]

Authors
C. Koehler, C. Ginzkey, G. Friehs, S. Hackenberg, K. Froelich, A. Scherzed, M. Burghartz, M. Kessler, N. Kleinsasser

Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Wuerzburg, Germany

 

The present study focuses on toxic effects of NO₂. Nasal epithelial mucosa cells were cultured on the air-liquid interface. After exposure, analysis of genotoxicity was performed by the comet assay and by the micronucleus test. 

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5. Aug. 2011

Sequential air-liquid exposure of human respiratory cells to chemical and biological pollutants

Toxicology Letters

2011 Nov 10;207(1):53-9. doi: 10.1016/j.toxlet.2011.07.028 [Epub ahead of print]

Authors
Persoz C, Leleu C., Achard S., Fasseu M., Menotti J., Meneceur P., Momas I., Derouin F., Seta N. Source

Université Paris Descartes, Laboratoire de Santé Publique et Environnement - EA 4064, Paris, France

 

The aim of this study is to manage air-liquid successive exposures to different pollutants such as a chemical pollutant and a biological contaminant using human alveolar cells (A549), which were exposed at the air-liquid interface. Inflammation biomarkers (IL-8, MCP-1) were assayed by ELISA and by RT-PCR.

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Comments (0) Number of views (1944)

10. Mar. 2011

In vitro exposure of organotypical 3D epithelial tissues to cigarette smoke as a potential alternative to rodent inhalation studies

SOT Washington DC, USA

March 06-10, 2011

Authors
Weisensee, D., Kurkowsky, B., Hebestreit, M., Wagner, S. and Schueller J. 

Philip Morris Research Laboratories GmbH, Cologne, Germany, Abstract #998

 

Here we describe an in vitro test system that examines the toxicological effects of single or repeated cigarette smoke exposure on human organo- typically reconstituted 3D epithelial tissues. These tissues were exposed at the air liquid interface to either fresh air or to mainstream smoke from the Reference Cigarette 3R4F in the VITROCELL® system. 

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