Advanced in vitro exposure systems.

7. Mar. 2010

Aspects of nitrogen dioxide toxicity in environmental urban concentrations in human nasal epithelium.

Toxicol Appl Pharmacol.

2010 Jun 1;245(2):219-25. doi: 10.1016/j.taap.2010.03.003. [Epub ahead of print]

Authors
Koehler C., Ginzkey C., Friehs G., Hackenberg S., Froelich K., Scherzed A., Burghartz M., Kessler M., Kleinsasser N.

Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Germany

 

Nasal epithelial cells of were cultured in an air-liquid interface and exposed to NO(2). After exposure, genotoxicity was evaluated by the Comet assay and the micronucleus test. Depression of proliferation and cytotoxic effects were determined using the micronucleus assay and trypan blue exclusion assay.

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3. Mar. 2010

Evaluation of Dicarbonyls Generated in a Simulated Indoor Air Environment Using an in Vitro Exposure System

Toxicol Sci.

2010 Jun;115(2):453-61. doi: 10.1093/toxsci/kfq067. [Epub ahead of print]

Authors
Anderson SE, Jackson LG, Franko J., Wells JR.

National Institute for Occupational Safety and Health (NIOSH), 1095 Willowdale Drive, Morgantown, WV 26505

 

The air-liquid interface was used to determine whether compounds formed from indoor chemistry were capable of inducing inflammatory cytokine expression by exposed pulmonary epithelial cells (A549). Results are shown by measured IL-6 and IL-8, granulocyte-macrophage colony-stimulating factor and tumor necrosis factor alpha.

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11. Sep. 2009

Biological effects in human lung cells exposed to platinum nanoparticle aerosol

European Aerosol Conference. Karlsruhe, Germany.

September 06-11, 2009

Authors
S. Diabaté, C. Weiss, S. Mülhopt, H.-R. Paur, V. Niedetzky, M. Seipenbusch

Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe, 76021, Karlsruhe, Germany
Institute for Technical Chemistry, Forschungszentrum Karlsruhe, 76021, Karlsruhe, Germany
Institute for Mechanical Process Engineering and Mechanics, University Karlsruhe, 76131, Karlsruhe, Germany

 

For the bioassay they exposed platinum nanoparticles to BEAS-2B and A549 cells, both co-cultured with differentiated THP-1 macrophages, growing on Transwell inserts. The responses of the cells were analyzed by measuring the viability, LDH and IL-8. 

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11. Sep. 2009

Toxic effects of nanoparticles from biomass combustion

European Aerosol Conference. Karlsruhe, Germany.

September 06-11, 2009

Authors
Werner Fr. Dreher, Frank Weise, Karen Böhme, Steffen Lutz, Michael Struschka, Johannes Brodbeck, Sonja Mülhopt, Hanns-Rudolf Paur, Silvia Diabaté

Materials Development and Testing, NMI Naturwissenschaftliches und Medizinisches Institut, Reutlingen, Germany
Institute of Process Engineering and Power Plant Technology (IVD), Universität Stuttgart, Stuttgart, Germany
Institute for Technical Chemistry, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany

 

The toxicity of particulate matters, from the incomplete combustion of biomass in fire places, will be investigated. A549 human lung cell cultures are subjected to a constant flow of a conditioned aerosol. After exposure, the responses of the cells are analysed to measure the biological responses such as viability, inflammatory or oxidative stress. 

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11. Sep. 2009

The QCM - Online Dose Measurement with high relative Humidity

European Aerosol Conference. Karlsruhe, Germany.

September 06-11, 2009

Authors
S. Mülhopt, A. Comouth, A. Grotz, T. Krebs, H.-R. Paur

Institute for Technical Chemistry, Thermal Waste Treatment Division, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
Institute for Meteorology and Climate Research, Atmospheric Aerosol Research Department, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
VITROCELL Systems GmbH, Waldkirch, Germany

 

The shown method first time provides an online dose measurement for in vitro exposure experiments at the air-liquid interface also under high humidity conditions.  Human lung cell cultures were tested for different biological responses like the viability (LDH, AlamarBlue) as well as the release of IL-8.

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3. Sep. 2009

Air/Liquid Culture and Exposure Technique: Preliminary Results of a Prevalidation Study

World Congress on Alternatives and Animal Use in the Life Sciences. Rome, Italy.

August 30 - September 3, 2009

Authors
Smirnova, L.1, Liebsch, M.1, Tharmann, J.1, Pirow, R.1, Luch, A.1, Bauer, M.2, Graebsch, C.2, Linsel, G.3, Siemers, R.3, Berger-Preiß, E.4, Kock, H.4, Oertel, A.4, Riiter, D.4, Knebel, J.4

1 Federal Institute for Risk Assessment (BfR), Unit 37, Centre for Alternative Methods to Animal Experiments, ZEBET, Berlin, Germany
2 Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany
3 Federal Institute for Occupational Safety and Health (BAuA), Berlin, Germany
4 Fraunhofer Institute of Toxicology and Experimental Medicine (ITEM), Hannover, Germany

 

The aim of this study is to determine the in vitro vs. in vivo dose-response relationships. A549 cell were grwon in the air-liquid interface and exposed to NO2, SO2, formaldehyde and ozone. The results are measured in cytotoxicity (viability) and genotoxicity (COMET assay). The project has proved satisfying. transferability of the test method.

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23. Mar. 2009

Lung toxicity determination by in vitro exposure at the air-liquid interface with an integrated online dose measurement

Nanosafe 2008: International Conference on Safe production and use of nanomaterials

Journal of Physics: Conference Series 170 (2009) 012008 (doi:10.1088/1742-6596/170/1/012008)

Authors
Sonja Mülhopt, S. Diabaté, T. Krebs, C. Weiss and H.-R. Paur

Institute for Technical Chemistry, Thermal Waste Treatment Division, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
VITROCELL Systems GmbH, 79183, Waldkirch, Germany

 

Human lung cell cultures are exposed to airborne nanoparticles in VITROCELL®system modules. After exposure the cells are analyzed to measure the biological responses of the viability (LDH, AlamarBlue) as well as the release of IL-8. Using fluorescein sodium particles shows a very good correlation between the sensor signal of the quartz crystal microbalance and the deposited mass on the membranes shown by spectroscopy. This system for the first time provides an online dose measurement for in vitro experiments with nanoparticles. 

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19. Mar. 2009

Identification of Indoor Air Contaminants Using an in Vitro Exposure System

SOT Baltimore, USA

March 15-19, 2009

Authors
Laurel G. Jackson, J. R. Wells, Jason Ham, Albert Munson, and Stacey E. Anderson

National Institute for Occupational Safety and Health, (CDC-NIOSH), Morgantown, USA

 

A549 cells were exposed to volatile organic compounds in the air-liquid interface. The results are measured in RNA isolation and  gene expression analysis.

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8. Oct. 2008

Online dose determination for in vitro experiments with nano particles in the Karlsruhe Exposure System

EUROTOX. Rhodes, Greece.

October 5-8, 2008

Authors
S. Mülhopt, H.-R. Paur, T. Krebs

Forschungszentrum Karlsruhe, Eggenstein – Leopoldshafen, Germany
VITROCELL Systems GmbH, Waldkirch, Germany

 

Poster about the exposition of airborne nanoparticles to human lung cell cultures in VITROCELL®system modules. After exposure the cells are analyzed to measure the biological responses of the viability (LDH, AlamarBlue) as well as the release of IL-8. Using fluorescein sodium particles shows a very good correlation between the sensor signal of the quartz crystal microbalance and the deposited mass on the membranes shown by spectroscopy. This system for the first time provides an online dose measurement for in vitro experiments with nanoparticles. 

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10. Mar. 2008

The Response of a Co-culture Lung Model to Fine and Ultrafine Particles of Incinerator Fly Ash at the Air-liquid Interface

ATLA

ATLA, Issue 36, pp. 285-298, 2008

Authors
Silvia Diabaté, Sonja Mülhopt, Hanns-Rudolf Paur and Harald F. Krug

Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein - Leopoldshafen, Germany

 

BEAS-2B cells were exposed to an aerosol, generated from fly ash. The cells were co-cultured with differentiated THP-1 macrophages growing on Transwell inserts. Analyses of cell viability and IL-8 release were performed.

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