Advanced in vitro exposure systems.

19. Sep. 2014

Silica nanoparticles are less toxic to human lung cells when deposited at the air-liquid interface compared to conventional submerged exposure

Beilstein Journal of Nanotechnology

2014, 5, 1590–1602 (doi:10.3762/bjnano.5.171)

Authors
Alicja Panas1, Andreas Comouth2, Harald Saathoff2, Thomas Leisner2, Marco Al-Rawi1, Michael Simon3, Gunnar Seemann3, Olaf Dössel3, Sonja Mülhopt4, Hanns-Rudolf Paur4, Susanne Fritsch-Decker1, Carsten Weiss1and Silvia Diabaté1

1 Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Campus North, Hermann- von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
2 Institute for Meteorology and Climate Research, Karlsruhe Institute of Technology, Campus North, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
3 Institute of Biomedical Engineering, Karlsruhe Institute of Technology, Campus South, Kaiserstraße 12, 76128 Karlsruhe, Germany
4 Institute for Technical Chemistry, Karlsruhe Institute of Technology, Campus North, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany

Exposition of humidified nanoparticles on A549 lung epithelia cells using the air-liquid interface. The experiments shows less cytotoxic and inflammatory responses of the cells to silica nanoparticles.

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21. Jul. 2014

In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures

informa healthcare

informa healthcare, posted online on July 21, 2014. (doi:10.3109/15376516.2014.943441)

Authors
Walter K. Schlage*, Anita R. Iskandar*, Radina Kostadinova*, Yang Xiang*, Alain Sewer*, Shoaib Majeed, Diana Kuehn, Stefan Frentzel, Marja Talikka, Marcel Geertz, Carole Mathis, Nikolai Ivanov, Julia Hoeng, and Manuel C. Peitsch

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

*Equal contribution

Exposition of cigarette smoke to tissue cultures at the air–liquid interface shows in combination of the microarray technology, gene-set analysis and a novel computational modeling an increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity. The usefulness of in vitro exposure to buccal and gingival organotypic epithelial tissue cultures for the impact assessment of cigarette smoke is demonstrated. 

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30. Jun. 2014

Transforming Growth Factor β1 and Cigarette Smoke Inhibit the Ability of β2-Agonists to Enhance Epithelial Permeability

www.atsjournals.org published on June 30, 2014 (doi: 10.1165/rcmb.2013-0538OC)

Authors
Hoshang J. Unwalla1, Pedro Ivonnet1, John S. Dennis, Gregory E. Conner1,2, and Matthias Salathe1
1Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, and
2Department of Cell Biology, University of Miami, Miller School of Medicine, Miami, Florida

Exposition of whole smoke on human bronchial epithelial cells in the air-liquid interface with attention on growth factor TGF-β1.

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21. Jun. 2014

A model of human nasal epithelial cells adapted for direct and repeated exposure to airborne pollutants

Toxicology Letters

2014 Jun 21;229(1):144-149. doi: 10.1016/j.toxlet.2014.05.023. Epub ahead of print.

Authors
Bardet G.1, Achard S.2, Loret T.3, Desauziers V.4, Momas I.5, Seta N.6

1 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France; Agence de l'Environnement et de la Maîtrise de l'Energie, Angers, France.
2 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France.
3 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France.
4 Centre des Matériaux des Mines d'Alès, Ecole des Mines d'Alès, Pau, France.
5 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France
6 Université Paris Descartes, EA 4064, Laboratoire de Santé Publique et Environnement, 4, Avenue de l'Observatoire, 75006 Paris, France; AP-HP, Hôpital Bichat, Biochimie, Paris, France.

The exposure to gaseous pollutants at the air-liquid interface shows an effect on the respiratory tract. The repeated exposition enables a model that gets closer to physiologically relevant conditions. Inflammatory marker IL-6 and IL-8, cellular viability and cytokine production were assessed.

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17. Jun. 2014

Development of a BALB/c 3T3 neutral red uptake cytotoxicity test using a mainstream cigarette smoke exposure system

BioMed Central

BMC Research Notes 2014, 7:367 (doi:10.1186/1756-0500-7-367)

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Linsey Haswell1, Annette Dalrymple1, Clive Meredith1 and Deborah Dillon1

1 British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

Mainstream cigarette smoke and the gase vapour phase, generated with the VC 10® smoke exposure system, indicates significant differently in-vitro cytotoxicity profiles. The used method on the air-liquid interface takes into account the synergies of both the particulate and vapour phase of tobacco smoke in the NRU assay.

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28. Apr. 2014

Efficient bioactive delivery of aerosolized drugs to human pulmonary epithelial cells cultured at air-liquid interface conditions

Am J Respir Cell Mol Biol

First published online 28 Apr 2014 (doi: 10.1165/rcmb.2013-0479OC)

Authors
Anke-Gabriele Lenz1, Tobias Stoeger1, Daniele Cei2, Martina Schmidmeir1, Nora Pfister1, Gerald Burgstaller1, Bernd Lentner1, Oliver Eickelberg3, Silke Meiners1 and Otmar Schmid1

1 Helmholtz Zentrum München, Institute of Lung Biology and Disease, Comprehensive Pneumology Center, Member of the German Center for Lung Research, Neuherberg/Munich, Germany
2 University of Pisa, Interdepartmental Research Center E.Piaggio”, Faculty of Engineering, Pisa, Italy
3,1 Ludwig-Maximilians-University, University Hospital, Munich, Germany

Introduction of the VITROCELL®-CLOUD (air-liquid interface cell exposure system-cloud) technology, which utilizes principles of cloud motion for fast and quantitative delivery of aerosolized liquid drugs to pulmonary cells cultured under realistic air-liquid interface conditions. These data indicate that the VITROCELL®-CLOUD is a reliable tool for aerosolized drug screening on cells.

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27. Mar. 2014

Potential toxicity of electronic cigarette liquids and aerosols as measured by four in vitro assays

SOT Phoenix, USA

24-27 March, 2014

Authors
Leverette, R.D., Misra, M., Cooper, B.T. and Bennett, M.B.

Lorillard Tobacco Company, A.W. Spears Research Center, Greensboro, NC 27405, USA

Exposition of tobacco and e-cigarette smoke, that were smoked on a VC 10® smoking robot in combination with a Vitrocell laser photometer for caculation of the delivered dose. The cytotoxicity was measured in four different endpoints: AMES, NRU, IL-8 Release and Micronucleus Assay.

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27. Mar. 2014

Cerium oxide nanoparticles air exposure: a comparison study using a human 3D airway model and A549 and Beas-2B cell lines

SOT Phoenix, USA

24-27 March, 2014

Authors
Ingeborg M. Kooter1, Mariska Grollers-Mulderij1, Maaike Steenhof1, Evert Duistermaat2, Frederique van Acker2, Yvonne Staal2, Eric Schoen1, Rob Stierum1, Linette Pellis1, Frieke Kuper1

1 The Netherlands Organisation for Applied Scientific Research, TNO, Zeist, The Netherlands
2 TNO Triskelion, Zeist, the Netherlands

Exposition of nano and macro particles on 3D lungcell models in comparison to normal lung cell lines in the air-liqid interface. Differentially effects are shown in the endpoints HO1, IL8, LDH, Comet Assay and gene expression.

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1. Nov. 2013

A review of in vitro cigarette smoke exposure systems

Experimental and Toxicologic Pathology

Volume 65, Issues 7-8, November 2013, Pages 1183-1193. pii: S0940-2993(13)00081-X. doi: 10.1016/j.etp.2013.06.001. [Epub ahead of print]

Authors
Thorne D, Adamson J.

British American Tobacco, Group R&D, Southampton SO15 8TL, UK.

In this review, the authors present a summary of the major tobacco smoke exposure systems available and critically review their function, set-up and application for in vitro exposure scenarios.

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30. Sep. 2013

Modelling and measurement of particle deposition for cell exposure at the air-liquid interface

Journal of Aerosol Science

Volume 63, September 2013, Pages 103-114

Authors
Andreas Comoutha, Harald Saathoffa, Karl-Heinz Naumanna, Sonja Muelhoptb, Hanns-Rudolf Paurb, Thomas Leisnera

a Institute for Meteorology and Climate Research, KIT, Karlsruhe, Germany
b Institute for Technical Chemistry, KIT, Karlsruhe, Germany

Presentation of a nanoparticle model to predict the size and particle deposition efficiencies for the air-liquid interface exposure system, that allows easy and accurate dose estimations. 

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