Advanced in vitro exposure systems.

31. Jan. 2017

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

DOI: 10.1089/aivt.2016.0025

Jason Adamson1,* Xiang Li2,* Huapeng Cui2, David Thorne1, Fuwei Xie2, and Marianna Gaca1
1British American Tobacco, R&D, Southampton, United Kingdom.
2Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, PR China.
*Joint first authors.

A VC 10 Smoking Robot was designated to look at the generated aerosol nicotine concentration from different e-cigarettes compared with the 3R4F reference cigarette in two different laboratories. The aerosol was trapped and the nicotine concentration was analysed by different kinds of mass spectometry.
Nicotine assessment across the tested e-cigarette categories showed consistent delivery of nicotine per puff within products and that the method was sensitive enough to detect different levels of nicotine across products. Good overlap in nicotine results were obtained in the two different laboratories.

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3. Jan. 2017

Development, qualification, validation and application of the neutral red uptake assay in Chinese Hamster Ovary (CHO) cells using a VITROCELL® VC10® smoke exposure system

Wanda Fields a, Kathy Fowler a, Victoria Hargreaves b, Lesley Reeve b, Betsy Bombick a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street,Winston-Salem, NC 27101, USA
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG1 3PY, UK

This study discribes the development, validation and application of the NRU assay in Chinese Hamster Ovary (CHO) cells, following exposure to fresh whole smoke generated with the VITROCELL®VC10®system. The VITROCELL® VC10® successfully provided consistent generation and delivery of whole smoke from a cigarette (3R4F) and a primarily “heat-not-burn” cigarette (Eclipse).

 

 

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16. Dec. 2016

'Clean-burning' Fuels May be Worse for Your Lungs

Nala Rogers for 'Inside Science'

A report about artificial lung reveals surprising dangers from pellet burners and diesel-fueled ships at the University of Rostock and Helmholtz Zentrum München in Germany.

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7. Dec. 2016

The comparative in vitro assessment of e-cigarette and cigarette smoke aerosols using the gH2AX assay and applied dose measurements

http://dx.doi.org/10.1016/j.toxlet.2016.12.006

David Thorne, Sophie Larard, Andrew Baxter, Clive Meredith, Marianna Gaҫa

British American Tobacco, R&D Centre, Southampton, Hampshire, SO15 8TL, UK

 

H I G H L I G H T S
Cigarette smoke generates a dose dependent increase in double strand breaks.
E-cigarette aerosols did not generate any increases in double strand breaks above control levels.
E-cigarette exposures were approximately 28-fold greater than that of cigarette smoke exposures.
In vitro dosimetry ensures accurate interpretation of the data, and confirms delivery of test agent.

In vitro dosimetry allows cross-product, study and system comparisons.

 

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3. Nov. 2016

Air-liquid interface exposure to aerosols of poorly soluble nanomaterials induces different biological activation levels compared to exposure to suspensions

DOI 10.1186/s12989-016-0171-3

Thomas Loret1,2, Emmanuel Peyret1, Marielle Dubreuil1, Olivier Aguerre-Chariol3, Christophe Bressot3, Olivier le Bihan3, Tanguy Amodeo3, Bénédicte Trouiller1, Anne Braun1, Christophe Egles2,4 and Ghislaine Lacroix1


1
Institut National de l’Environnement Industriel et des Risques (INERIS), (DRC/VIVA/TOXI), Parc Technologique ALATA—BP 2, Verneuil-en-Halatte F-60550, France.
2Laboratoire BioMécanique et BioIngénierie (BMBI), Université de Technologie de Compiègne (UTC), UMR CNRS 7338, Compiègne 60205, France.
3Institut National de l’Environnement Industriel et des Risques (INERIS), (DRC/CARA/NOVA), Parc Technologique ALATA—BP 2, Verneuil-en-Halatte F-60550, France.
4Department of Biomedical Engineering, Tufts University, Medford, MA, USA.
 

A549 alveolar cells in monocultures and in co-cultures with THP-1 macrophages were exposed to aerosols of inhalable and poorly soluble nanomaterials. The results are evaluated by using the Alamar blue®, LDH, Dichlorofluorescein (DCF) and ELISA Assay and shows that the air-liquid interface represents a valid and sensitive method to assess the toxicity of several poorly soluble nanomaterials.

 

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31. Oct. 2016

Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

doi: 10.1155/2016/9461289
Patrick Geraghty,1,2 Nathalie Baumlin,3 Matthias A. Salathe,3 Robert F. Foronjy,1,2 and Jeanine M. D’Armiento4
1Division of Pulmonary & Critical Care Medicine, Department of Medicine, State University of New York Downstate Medical Center, Brooklyn, NY, USA
2Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY, USA
3Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Miami, Miami, FL, USA
4Center for Pulmonary Disease, Department of Anesthesiology, College of Physicians and Surgeons,Columbia University, New York, NY, USA

 

The Expression of ER stress markers after exposure to cigarette smoke was investigated in fully differentiated normal human bronchial epithelial (NHBE) cells isolated from nonsmoking, smoking, and COPD donors and redifferentiated at the air liquid interface.

 

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8. Sep. 2016

A lab scale measurement technique for the air-liquid interface exposure of human lung cell cultures towards particulate emissions from combustion processes

11th International Particle Toxicology Conference, Sep 26 – 30, 2016 in Singapore


1S. Mülhopt, 3M. Berger, 1C. Schlager, 2M. Dilger, 2S. Diabaté, 3T. Krebs, 4,5Ralf Zimmermann, 4Jeroen Buters, 4Sebastian Oeder, 2C. Weiss, 1H.-R. Paur

1Institute for Technical Chemistry
2Institute of Toxicology and Genetics

3Vitrocell Systems GmbH, Germany
4Helmholtz CentreMunich, Germany
5University of Rostock, Germany
 

The Karlsruhe Exposure System is a lab scale measurement system for the air-liquid interface exposure of human lung cell cultures towards air borne nanoparticles under well defined conditions.

 

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27. Jun. 2016

Metabolic Profiling as Well as Stable Isotope Assisted Metabolic and Proteomic Analysis of RAW 264.7 Macrophages Exposed to Ship Engine Aerosol Emissions

Different Effects of Heavy Fuel Oil and Refined Diesel Fuel

DOI:10.1371/journal.pone.0157964

Sean C. Sapcariu1,12, Tamara Kanashova2,12, Marco Dilger3,4,12, Silvia Diabaté3,12, Sebastian Oeder5,6,12,13, Johannes Passig7,12, Christian Radischat7,12, Jeroen Buters5,6,12,13, Olli Sippula8,12, Thorsten Streibel7,9,12, Hanns-Rudolf Paur4,12, Christoph Schlager4,12, Sonja Mülhopt4,12, Benjamin Stengel10,12, Rom Rabe10,12, Horst Harndorf10,12, Tobias Krebs11,12, Erwin Karg9, Thomas Gröger9, Carsten Weiss3,12, Gunnar Dittmar2,12, Karsten Hiller1,12, Ralf Zimmermann7,9,12

1 Luxembourg Centre for Systems Biomedicine 6, avenue du Swing, L-4362 Esch-sur-Alzette, Luxembourg,
2 Mass Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine Berlin-Buch, Berlin, Germany,
3 Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
4 Institute for Technical Chemistry (ITC), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
5 Center of Allergy and Environment (ZAUM), Helmholtz Zentrum München and Technische Universität München, Munich, Germany,
6 CK-CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland,
7 Joint Mass Spectrometry Centre, Division of Analytical and Technical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany,
8 University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland,
9 Joint Mass Spectrometry Centre, CMA – Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany,
10 Chair of Piston Machines and Internal Combustion Engines, University Rostock, Rostock, Germany,
11 Vitrocell GmbH, Waldkirch, Germany,
12 HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany; Kuopio, Finland; Cardiff, United Kingdom; Esch-Belval, Luxembourg,
13 German Center for Lung Research (DZL), Munich, Germany

An automated ALI exposure system station with 18 exposure positions was used as the interface for mouse macrophage RAW 264.7 cell line exposures of the diesel engine exhaust. The evaluation is devided in LDH release assay, Metabolite extraction and GC-MS processing, Stable isotope labeling by amino acids in cell culture (SILAC), Proteome extraction and LC-MS/MS analysis of peptides and Proteomics Data An

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16. Jun. 2016

Nanoparticles on human lung cells

The Vitrocell Exposure System for cell cultures at the air-liquid interface

Sonja Mülhopt1, Tobias Krebs2, Dr Silvia Diabaté3, Christoph Schlager1, Dr Hanns-Rudolf Paur1

1 Institute for Technical Chemistry, Karlsruhe Institute of Technology, 
2 Vitrocell Systems GmbH, 
3 Institute of Toxicology and Genetics, Karlsruhe Institute of Technology

 

This article shows the development of an automated exposure system which allows both reproducible sampling and conditioning of aerosols and exposure of the cell cultures under conditions imitating those of the human lung. Bioassays were developed and used for toxicological analysis of particulate emissions from the industry as well as of nanoparticles.

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4. Apr. 2016

Cutting-edge in vitro Exposure Technologies for Conventional and E-cigarettes

IIVS Workshop. April 4-6, 2016. Washington

GAITHERSBURG, MD – April 12, 2016 – The Institute for In Vitro Sciences (IIVS) brought together stakeholders from industry, government and academia to discuss considerations for exposure and dosimetry for non-animal testing during its 2.5-day workshop, “In Vitro Exposure Systems and Dosimetry Assessment Tools for Inhaled Tobacco Products”, held April 4-6, 2016 in Bethesda, MD. Uniquely focused on in vitro testing, the workshop is the only known event of its kind. VITROCELL Systems had the opportunity to present Cutting-edge in vitro Exposure Technologies for Conventional and E-cigarettes.

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