Advanced in vitro exposure systems.

27. Jun. 2016

Metabolic Profiling as Well as Stable Isotope Assisted Metabolic and Proteomic Analysis of RAW 264.7 Macrophages Exposed to Ship Engine Aerosol Emissions

Different Effects of Heavy Fuel Oil and Refined Diesel Fuel

DOI:10.1371/journal.pone.0157964

Sean C. Sapcariu1,12, Tamara Kanashova2,12, Marco Dilger3,4,12, Silvia Diabaté3,12, Sebastian Oeder5,6,12,13, Johannes Passig7,12, Christian Radischat7,12, Jeroen Buters5,6,12,13, Olli Sippula8,12, Thorsten Streibel7,9,12, Hanns-Rudolf Paur4,12, Christoph Schlager4,12, Sonja Mülhopt4,12, Benjamin Stengel10,12, Rom Rabe10,12, Horst Harndorf10,12, Tobias Krebs11,12, Erwin Karg9, Thomas Gröger9, Carsten Weiss3,12, Gunnar Dittmar2,12, Karsten Hiller1,12, Ralf Zimmermann7,9,12

1 Luxembourg Centre for Systems Biomedicine 6, avenue du Swing, L-4362 Esch-sur-Alzette, Luxembourg,
2 Mass Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine Berlin-Buch, Berlin, Germany,
3 Institute of Toxicology and Genetics (ITG), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
4 Institute for Technical Chemistry (ITC), Karlsruhe Institute of Technology, Campus North, Karlsruhe, Germany,
5 Center of Allergy and Environment (ZAUM), Helmholtz Zentrum München and Technische Universität München, Munich, Germany,
6 CK-CARE, Christine Kühne Center for Allergy Research and Education, Davos, Switzerland,
7 Joint Mass Spectrometry Centre, Division of Analytical and Technical Chemistry, Institute of Chemistry, University Rostock, Rostock, Germany,
8 University of Eastern Finland, Department of Environmental Science, P.O. Box 1627, FI-70211 Kuopio, Finland,
9 Joint Mass Spectrometry Centre, CMA – Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany,
10 Chair of Piston Machines and Internal Combustion Engines, University Rostock, Rostock, Germany,
11 Vitrocell GmbH, Waldkirch, Germany,
12 HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health, Neuherberg, Rostock, Munich, Karlsruhe, Berlin, Waldkirch, Germany; Kuopio, Finland; Cardiff, United Kingdom; Esch-Belval, Luxembourg,
13 German Center for Lung Research (DZL), Munich, Germany

An automated ALI exposure system station with 18 exposure positions was used as the interface for mouse macrophage RAW 264.7 cell line exposures of the diesel engine exhaust. The evaluation is devided in LDH release assay, Metabolite extraction and GC-MS processing, Stable isotope labeling by amino acids in cell culture (SILAC), Proteome extraction and LC-MS/MS analysis of peptides and Proteomics Data An

Read more

Comments (0) Number of views (1233)

16. Jun. 2016

Nanoparticles on human lung cells

The Vitrocell Exposure System for cell cultures at the air-liquid interface

Sonja Mülhopt1, Tobias Krebs2, Dr Silvia Diabaté3, Christoph Schlager1, Dr Hanns-Rudolf Paur1

1 Institute for Technical Chemistry, Karlsruhe Institute of Technology, 
2 Vitrocell Systems GmbH, 
3 Institute of Toxicology and Genetics, Karlsruhe Institute of Technology

 

This article shows the development of an automated exposure system which allows both reproducible sampling and conditioning of aerosols and exposure of the cell cultures under conditions imitating those of the human lung. Bioassays were developed and used for toxicological analysis of particulate emissions from the industry as well as of nanoparticles.

Read more

Comments (0) Number of views (1412)

4. Apr. 2016

Cutting-edge in vitro Exposure Technologies for Conventional and E-cigarettes

IIVS Workshop. April 4-6, 2016. Washington

GAITHERSBURG, MD – April 12, 2016 – The Institute for In Vitro Sciences (IIVS) brought together stakeholders from industry, government and academia to discuss considerations for exposure and dosimetry for non-animal testing during its 2.5-day workshop, “In Vitro Exposure Systems and Dosimetry Assessment Tools for Inhaled Tobacco Products”, held April 4-6, 2016 in Bethesda, MD. Uniquely focused on in vitro testing, the workshop is the only known event of its kind. VITROCELL Systems had the opportunity to present Cutting-edge in vitro Exposure Technologies for Conventional and E-cigarettes.

Read more

Comments (0) Number of views (1261)

14. Mar. 2016

Cellular Effects in an In Vitro Human 3D Cellular Airway Model and A549/BEAS-2B In Vitro Cell Cultures Following Air Exposure to Cerium Oxide Particles at an Air–Liquid Interface

DOI: 10.1089/aivt.2015.0030

Ingeborg M. Kooter,1 Mariska Gröllers-Mulderij,1 Maaike Steenhof,1 Evert Duistermaat,2 Frederique A.A. van Acker,2 Yvonne C.M. Staal,2 Peter C. Tromp,1 Eric Schoen,1 C. Frieke Kuper,1 and Eugene van Someren1

1The Netherlands Organisation for Applied Scientific Research, TNO, Utrecht, The Netherlands.
2TNO Triskelion BV, Zeist, The Netherlands.

BEAS-2B, A549, and MucilAir cells were exposed to CeO2 particles and are cultured at air–liquid interface. LDH, cytokine analysis, gene expression analyses, HO-1 analyses and Comet assay were used for evaluation. Cell responses in the MucilAir model exposed via air to CeO2 were minimal compared to those of the cell lines.

Read more

Comments (0) Number of views (1064)

9. Mar. 2016

Toxicity testing of combustion aerosols at the air-liquid interface with a self-contained and easy-to-use exposure system

doi:10.1016/j.jaerosci.2016.02.005

Sonja Mülhopt a, i, Marco Dilger a, b, i, Silvia Diabaté b, i, Christoph Schlager a, i, Tobias Krebs c, i, Ralf Zimmermann d, h, i, Jeroen Buters e, i, Sebastian Oeder e, g, i, Thomas Wäscher f, Carsten Weiss b, i, Hanns-Rudolf Paur a, i

a Karlsruhe Institute of Technology, Institute for Technical Chemistry, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
b Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
c Vitrocell Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany
d University of Rostock, Institute of Chemistry, Dr.-Lorenz-Weg 1, 18051 Rostock, Germany
e Center of Allergy & Environment (ZAUM), Technische Universität and Helmholtz Zentrum München, Biedersteiner Str. 29, 80802 München, Germany
f Ingenieurbüro für Energie- und Verfahrenstechnik, Von-Dalheim-Str. 2, 69231 Rauenberg, Germany
g Kühne Foundation, Christine Kühne Center for Allergy Research and Education (CK- CARE), München, Germany
h Cooperation Group “Comprehensive Molecular Analytics” – CMA, Helmholtz Zentrum München, 85764 Oberschleißheim/ Germany
i HICE – Helmholtz Virtual Institute of Complex Molecular Systems in Environmental Health – Aerosols and Health

 

Highlights

• We present a fully automated Air-Liquid-Interface Exposure System.
• System characterisation shows a high reproducibility.
• Wood combustion aerosol causes cytotoxicity in human lung epithelial cells.
• Gene regulation caused by ship diesel emissions in human lung epithelial cells.

Read more

Comments (0) Number of views (1535)

8. Jan. 2016

Carbonbeton – eine neue Art des Bauens

Projekt C³-Carbon Concrete Composite

VITROCELL® Systems GmbH ist Teil dieses größten Bauforschungsprojektes Deutschlands, dem Projekt C³-Carbon Concrete Composite. Zusammen mit mehr als 130 Partnern aus Forschung, Unternehmen und Verbänden wird im C³-Projekt einen neuer Materialverbund aus Carbonfasern und Hochleistungsbeton mit unserer Technologie getestet.

Read more

Comments (0) Number of views (3071)

31. Oct. 2015

Roflumilast partially reverses smoke-induced mucociliary dysfunction

DOI 10.1186/s12931-015-0294-3

Andreas Schmid, Nathalie Baumlin, Pedro Ivonnet, John S. Dennis, Michael Campos, Stefanie Krick and Matthias Salathe*

* Correspondence: msalathe@med.miami.edu
Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Miami Miller School of Medicine, 1600 NW 10th Ave, RMSB #7058, Miami, FL 33136, USA

 

Primary normal human bronchial epithelial cells (NHBE) from non-smokers, cultured at the air-liquid interface (ALI), were exposed to cigarette smoke in a Vitrocell VC-10 smoking robot. The results show that roflumilast can rescue smoke-induced mucociliary dysfunction.Therefor the ELIZA Assay and qPCR were used.

Read more

Comments (0) Number of views (1444)

29. Sep. 2015

Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure

DOI 10.1186/s12989-015-0104-6

Linda C. Stoehr1,2†, Carola Endes3†, Isabella Radauer-Preiml1, Matthew S. P. Boyles1, Eudald Casals4, Sandor Balog5, Markus Pesch2, Alke Petri-Fink3, Barbara Rothen-Rutishauser3, Martin Himly1, Martin J. D. Clift3 and Albert Duschl1*

1Department of Molecular Biology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria.

2Grimm Aerosol Technik GmbH & Co. KG, Ainring, Germany.
3BioNanomaterials, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
4Institut Català de Nanotecnologia (ICN), Bellaterra, Spain.
5Soft Matter Scattering, Adolphe Merkle Institute, Université de Fribourg, Fribourg, Switzerland.
* Correspondence: albert.duschl@sbg.ac.at


The use of fluorescence-based reporter cell lines can provide a useful tool in screening the pro-inflammatory response following NP exposure in the air-liquid interface. The results are shown in the IL-8 assay, qRT-PCR ans ELISA.

Read more

Comments (0) Number of views (1639)

23. Sep. 2015

Airway epithelium co-cultured with immune cells for a better assessment of the low dose effects of environmental pollutants

EUROTOX 13.-16. September 2015

Authors

Emilie RICQUEBOURG, Gabrielle VERNOUILLET, Isabelle MOMAS, Nathalie SETA, Sophie ACHARD

Public Health and Environment Laboratory, EA4064 - Paris Descartes University


Exposure of airborne pollutants to a 3D airway epithelial cell modell in the air-liquid interface to measure the epithelial thickness and the inflammatory respone with the IL-8 assay.

Winner of the price "AstraZeneca Young Investigator Prize for Innovation in Drug Discovery and Development"

Read more

Comments (0) Number of views (1417)

23. Sep. 2015

A comparative assessment of cigarette smoke aerosols using an in vitro air-liquid interface cytotoxicity test.

DOI:10.3109/08958378.2015.1080773

Authors

Thorne D, Dalrymple A, Dillon D, Duke M, Meredith C

British American Tobacco, Group R&D, Southampton, Hampshire, UK.

 

Evaluation of a modified air-liquid interface BALB/c 3T3 cytotoxicity method for the assessment of smoke aerosols in vitro. 

Read more

Comments (0) Number of views (1322)
RSS
First234567891011Last
Back to Top