Lukasz Czekala1, Matthew Stevenson1, Liam Simms1, Nicole Tschierske1, Anna G. Maione2, Tanvir Walele3
1 Imperial Tobacco Ltd, 121 Winterstoke Road, Bristol, BS3 2LL UK
2 MatTek, 200 Homer Ave., Ashland, MA, USA
3 Fontem Ventures B.V., an Imperial Brands PLC Company, Radarweg60, 1043 NT Amsterdam
A 3D in vitro organotypic model of the human respiratory epithelium was cultured at the Air-Liquid Interface (ALI) and exposed to cigarette smoke and e-cigarette aerosol, generated by using a VITROCELL® VC1 smoking machine under Health Canada Intense and CORESTA conditions.
The results are shown in tissue viability (MTT), barrier integrity (TEER), Barrier function, tissue secretion of the pro-inflammatory cytokines (IL-6 and IL-8). The presence of 8-isoprostane is used as an indicator of oxidative stress and antioxidant deficiency. Additionally the tissue morphology was assessed by H&E staining and immunofluorescent staining was conducted for specific markers.