Savvina Chortarea,1 Hana Barosova,1 Martin James David Clift,2 Peter Wick,3 Alke Petri-Fink,1,4 and Barbara Rothen-Rutishauser,1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, CH-1700 Fribourg, Switzerland
2In Vitro Toxicology Group, Swansea University Medical School, Swansea SA2 8PP, Wales, U.K.
3Laboratory for Materials-Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science and Technology, 9014 St Gallen, Switzerland
4Department of Chemistry, University of Fribourg, CH-1700 Fribourg, Switzerland
The aim of this study was to realistically mimic subchronic inhalation of multiwalled carbone nanotubes in vitro, using the air−liquid interface cell exposure system for aerosol exposures on reconstituted human bronchial tissue from healthy and asthmatic donors. Cytotoxicity, cell morphology, cilia beating frequency, mucociliary clearance, gene expression analysis of oxidative stress and (pro‑)inflammatory markers as well as (pro‑)inflammatory cytokine secretion were successfully observed.