Improved Sensor for High Precision Real-Time Dose Monitoring

The VITROCELL® sQCM 12 is the next step in the evolution of dosimetry methods in cell culture exposure systems. It simplifies the usage of conventional Quartz Crystal Microbalances suitable for VITROCELL® 12 modules while maintaining the working principle, precision and accuracy of the balances you’re used to.

VITROCELL® S-QCM

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https://doi.org/10.1016/j.envint.2021.106718

Rui-Wen He ^a,b, Marc M.G. Houtzager ^c, W.P. Jongeneel ^a, Remco H.S. Westerink ^b, Flemming R. Cassee ^a,b

^a National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, the Netherlands
^b Institute for Risk Assessment Sciences (IRAS), Toxicology Division, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80177, 3508 TD Utrecht, the Netherlands
^c The Netherlands Organisation for Applied Scientific Research, TNO, P.O. Box 80015, 3508 TA Utrecht, the Netherlands

This unique experimental “Mini-BACS + AES” setup is able to provide steady conditions to perform in vitro exposure under ALI conditions to aircraft engine oil and hydraulic fluid fumes, generated at respectively 350 ◦C and 200 ◦C. Exposure of the Calu-3 monoculture and Calu-3 + MDM co-culture lung cell models to high levels of aircraft engine oil and hydraulic fluid fumes under ALI conditions can reduce TEER and viabilities of the cells, induce cytotoxicity, and increase production of proinflammatory cytokines. Hydraulic fluid fumes are more toxic than engine oil fumes on the mass concentration of fume basis, which may be related to higher abundance of OPs and smaller particle size of hydraulic fluid fumes. The toxicological data clearly reflect the potential health risks during fume events in aircraft cabins.

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https://doi.org/10.1016/j.jes.2021.06.001

Ana Teresa Juarez-Facio ¹, Clément Castilla ², Cécile Corbière ¹, Hélène Lavanant ², Carlos Afonso ², Christophe Morin ², Nadine Merlet-Machour ², Laurence Chevalier ³, Jean-Marie Vaugeois ¹, Jérôme Yon ⁴, Christelle Monteil ¹,
¹Normandie Univ, UNIROUEN, UNICAEN ABTE, 76000 Rouen, France
²Normandie Univ, UNIROUEN, INSA Rouen, CNRS, COBRA, 76000 Rouen, France
³Normandie Univ, UNIROUEN, INSA Rouen, CNRS, GPM-UMR6634, 76000 Rouen, France
⁴Normandie Univ, UNIROUEN, INSA Rouen, CNRS, CORIA, 76000 Rouen, France

The present study illustrate a methodological approach which allows comparing, in an original and innovative way, the physical, chemical, and toxicological properties of different fine and ultrafine particles. In this study, two different operating conditions were used to obtain model particles containing either high (CAST3) or low (CAST1) organic contents, and exposed differentiated NHBE cells “on-line” at the air-liquid interface to be closer to realistic conditions.

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How to use Millicell 24-well Standing Inserts in the VITROCELL® 12 Module Series

The revolutionary new holder system enables for the use of standing inserts in all modules of the VITROCELL® 12 Series for 12-well sized inserts. As an additional advantage, the media com­partment is sealed towards the aerosol exposure head to avoid contact of the test substance with the cell culture media.

VITROCELL® Millicell Holder – How to use Millicell 24-well Standing inserts in the VITROCELL® 12 Module Series

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doi:10.3390/rs12040648

Patrick Augustin ¹, Sylvain Billet ² , Suzanne Crumeyrolle ³, Karine Deboudt ¹, Elsa Dieudonné ¹ , Pascal Flament ¹ , Marc Fourmentin ¹ , Sarah Guilbaud ¹, Benjamin Hanoune ⁴ , Yann Landkocz ², Clémence Méausoone ², Sayahnya Roy ⁵, François G. Schmitt ⁵, Alexei Sentchev ⁵ and Anton Sokolov ^1
1 Univ. Littoral Côte d’Opale, UR 4493—LPCA—Laboratoire de Physico-Chimie de l’Atmosphère, 59140 Dunkerque, France;
² Univ. Littoral Côte d’Opale, SFR Condorcet FR CNRS 3417, UR 4492—UCEiV—Unité de Chimie Environnementale et Interactions sur le Vivant, 59140 Dunkerque, France; 
³ Univ. Lille, CNRS, UMR 8518—LOA—Laboratoire d’Optique Atmosphérique, 59000 Lille, France;
⁴ Univ. Lille, CNRS, UMR 8522—PC2A—Physico-Chimie des Processus de Combustion et de l’Atmosphère, 59000 Lille, France; 
⁵ Univ. Lille, Univ. Littoral Côte d’Opale, CNRS, UMR 8187—LOG—Laboratoire d’Océanologie et de Géosciences, F 62930 Wimereux, France; 

An atmospheric mobile unit was implemented during a field campaign performed at a representative site of urbanized and industrialized coastal environment of the North Sea (Northern France), to study the impact of sea breeze dynamics on aerosol properties, and especially their toxicity. This unit combined aerosol samplers, two scanning lidars (Doppler and elastic), two aerosol particle sizers and an air-liquid interface (ALI, Vitrocell) in vitro cell exposure device. This study is one of the first to bring cell cultures into the field to evaluate the harmfulness of a real environmental compartment. Atmospheric toxicity in the presence or absence of sea breeze was demonstrated in human bronchial cells exposed in the field using an ALI exposure system. The study showed the sensitivity of the developed device to discriminate the mechanisms of toxic action activated when exposed to different atmospheres.

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