DOI: 10.1021/acsnano.9b01823

Ingeborg Kooter¹, Marit Ilves ², Mariska Gröllers-Mulderij ¹, Evert Duistermaat ³, Peter C. Tromp ¹, Frieke Kuper ¹, Pia Kinaret ^4,5, Kai Savolainen ⁶, Dario Greco ^4,5, Piia Karisola ², Joseph Ndika ², and Harri Alenius <sup>2,7

1</sup>The Netherlands Organization for Applied Scientific Research, TNO, P.O. Box 80015, Utrecht 3584 CB, The Netherlands
²Human Microbiome Research, Faculty of Medicine, University of Helsinki, P.O. Box 21, Helsinki 00290, Finland
³Triskelion B.V., P.O. Box 844, Zeist 3704 HE, The Netherlands
⁴Faculty of Medicine and Life Sciences, University of Tampere, Tampere FI-33014, Finland
⁵Institute of Biotechnology, University of Helsinki, P.O. Box 56, Helsinki 00014, Finland
⁶Finnish Institute of Occupational Health, P.O. Box 40, Helsinki 00014, Finland
⁷Institute of Environmental Medicine, Karolinska Institutet, P.O. Box 210, Stockholm SE-17176, Sweden

3D human bronchial epithelial cells were cultured at the air−liquid interface that mimics relevant inhalatory exposure were exposed to aerosols of pristine (nCuO) and carboxylated (nCuOCOOH) copper oxide nanoparticles. This paper shows that the existence of asthma enhances sensitivity of the airways to nanoparticle aerosols, possibly as a combined result of a hyperactive airway and inefficient mucociliary clearance mechanisms in asthmatics. The test results are shown in cell viabilty (LDH), Inflammation (IL6, IL8, MCP1) and Transcroptomics.

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DOI: 10.1016/j.tiv.2019.03.030
Méausoone C¹, El Khawaja R¹, Tremolet G¹, Siffert S¹, Cousin R¹, Cazier F², Billet S¹, Courcot D³, Landkocz Y¹.
¹ UCEIV - EA4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.
² Centre Commun de Mesure, Université du Littoral Côte d'Opale, Dunkerque, France.
³ UCEIV - EA4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.. Electronic address: dominique.courcot@univ-littoral.fr.

BEAS-2B cells were exposed using an Air-Liquid Interface (ALI) System (Vitrocell®) to gaseous flows of toluene vapors and outflow after catalytic oxidation of toluene. After exposure to gaseous flow, cytotoxicity, inflammatory response and Xenobiotic Metabolism Enzymes (XME) gene expression were investigated.

Highlights
• Toxicity of toluene increases with time when cells are repeatedly exposed.
• Toxicological analysis helps for validation of catalysts used in treatment of VOCs.
• Cellular response supports the identification of chemically undetected by-products.

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DOI:10.1007/s11739-019-02055-x
Filippo Zanetti¹, Athanasios Kondylis¹, Florian Martin¹, Patrice Leroy¹, Shoaib Majeed¹, Sandro Steiner¹, Yang Xiang¹, Laura Ortega Torres¹, Keyur Trivedi¹, Emmanuel Guedj¹, Celine Merg¹, Stefan Frentzel1, Nikolai V. Ivanov¹, Utkarsh Doshi², Kyeonghee Monica Lee², Willie J. McKinneyJr², Manuel C. Peitsch¹, Julia Hoeng¹

¹ Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
² Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

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DOI: 10.1016/j.yrtph.2019.01.036

Czekala L¹, Simms L², Stevenson M², Tschierske N², Maione AG³, Walele T².
¹ Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK. Electronic address: Lukasz.Czekala@uk.imptob.com.
² Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK.
³ MatTek, 200 Homer Ave., Ashland, MA, USA.

In this research article a respiratory epithelial model, EpiAirway, was used to examine the biological effects of nicotine-containing blu PLUS + e-cigarettes, in comparison to conventional cigarette smoke. Tissues were exposed at the air-liquid interface to cigarette smoke or e-cigarette aerosol generated using a VITROCELL VC1 smoking/vaping robot. 

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DOI: 10.1089/jamp.2017.1364

Ivana d’Angelo, PhD,¹ Gabriella Costabile, PhD,^2,3 Estelle Durantie, PhD,³ Paola Brocca, PhD,⁴ Valeria Rondelli, PhD,⁴ Annapina Russo, PhD,⁵ Giulia Russo, PhD,⁵ Agnese Miro, PharmD,² Fabiana Quaglia, PhD,² Alke Petri-Fink, PhD,³ Barbara Rothen-Rutishauser, PhD,³ and Francesca Ungaro, PhD^2
1 Di.S.T.A.Bi.F., University of Campania ‘‘Luigi Vanvitelli,’’ Caserta, Italy.
² Laboratory of Drug Delivery, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.
³ Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
⁴ Applied Physics, Department of Medical Biotechnology and Translational Medicine, University of Milano, Milano, Italy.
⁵ Laboratory of Biochemistry, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.

The Vitrocell Cloud was used for the exposure to aerosolized nanoparticles on a in vitro three-dimensional cell cluture, mimicking the human epithelial airway barrier comprising human bronchial epithelial cells,human blood monocyte-derived macrophages and dendritic cells to show the optimized siRNA transport and delivery at lung.

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