DOI: 10.1016/j.tiv.2019.03.030
Méausoone C¹, El Khawaja R¹, Tremolet G¹, Siffert S¹, Cousin R¹, Cazier F², Billet S¹, Courcot D³, Landkocz Y¹.
¹ UCEIV - EA4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.
² Centre Commun de Mesure, Université du Littoral Côte d'Opale, Dunkerque, France.
³ UCEIV - EA4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, Dunkerque, France.. Electronic address: dominique.courcot@univ-littoral.fr.

BEAS-2B cells were exposed using an Air-Liquid Interface (ALI) System (Vitrocell®) to gaseous flows of toluene vapors and outflow after catalytic oxidation of toluene. After exposure to gaseous flow, cytotoxicity, inflammatory response and Xenobiotic Metabolism Enzymes (XME) gene expression were investigated.

Highlights
• Toxicity of toluene increases with time when cells are repeatedly exposed.
• Toxicological analysis helps for validation of catalysts used in treatment of VOCs.
• Cellular response supports the identification of chemically undetected by-products.

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DOI:10.1007/s11739-019-02055-x
Filippo Zanetti¹, Athanasios Kondylis¹, Florian Martin¹, Patrice Leroy¹, Shoaib Majeed¹, Sandro Steiner¹, Yang Xiang¹, Laura Ortega Torres¹, Keyur Trivedi¹, Emmanuel Guedj¹, Celine Merg¹, Stefan Frentzel1, Nikolai V. Ivanov¹, Utkarsh Doshi², Kyeonghee Monica Lee², Willie J. McKinneyJr², Manuel C. Peitsch¹, Julia Hoeng¹

¹ Philip Morris International R&D, Philip Morris Products S.A.NeuchâtelSwitzerland
² Altria Client Services LLCRichmondUSA

Human organotypic buccal and small airway cultures were exposed in two independent exposure systems (Vitrocell® 24/48), one for 3R4F reference cigarette smoke and the other for e-cigarette aerosol exposures to summarize the exposure-induced impacts into four main cellular processes: the cell fate, cell proliferation, cell stress, and inflammatory process.

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DOI: 10.1016/j.yrtph.2019.01.036

Czekala L¹, Simms L², Stevenson M², Tschierske N², Maione AG³, Walele T².
¹ Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK. Electronic address: Lukasz.Czekala@uk.imptob.com.
² Imperial Brands PLC, 121 Winterstoke Road, Bristol, BS3 2LL, UK.
³ MatTek, 200 Homer Ave., Ashland, MA, USA.

In this research article a respiratory epithelial model, EpiAirway, was used to examine the biological effects of nicotine-containing blu PLUS + e-cigarettes, in comparison to conventional cigarette smoke. Tissues were exposed at the air-liquid interface to cigarette smoke or e-cigarette aerosol generated using a VITROCELL VC1 smoking/vaping robot. 

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DOI: 10.1089/jamp.2017.1364

Ivana d’Angelo, PhD,¹ Gabriella Costabile, PhD,^2,3 Estelle Durantie, PhD,³ Paola Brocca, PhD,⁴ Valeria Rondelli, PhD,⁴ Annapina Russo, PhD,⁵ Giulia Russo, PhD,⁵ Agnese Miro, PharmD,² Fabiana Quaglia, PhD,² Alke Petri-Fink, PhD,³ Barbara Rothen-Rutishauser, PhD,³ and Francesca Ungaro, PhD^2
1 Di.S.T.A.Bi.F., University of Campania ‘‘Luigi Vanvitelli,’’ Caserta, Italy.
² Laboratory of Drug Delivery, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.
³ Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
⁴ Applied Physics, Department of Medical Biotechnology and Translational Medicine, University of Milano, Milano, Italy.
⁵ Laboratory of Biochemistry, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.

The Vitrocell Cloud was used for the exposure to aerosolized nanoparticles on a in vitro three-dimensional cell cluture, mimicking the human epithelial airway barrier comprising human bronchial epithelial cells,human blood monocyte-derived macrophages and dendritic cells to show the optimized siRNA transport and delivery at lung.

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DOI: 10.2217/nnm-2017-0358

Savvina Chortarea^1,2, Kleanthis Fytianos^1,3,4, Laura Rodriguez-Lorenzo¹, Alke Petri-Fink^1,5 & Barbara Rothen-Rutishauser^1
1BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
²Laboratory for Particles – Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
³Department of Pulmonary Medicine, University of Bern, Bern, Switzerland
⁴Department of Clinical Research, University of Bern, Bern, Switzerland
⁵Department of Chemistry, University of Fribourg, Fribourg, Switzerland

The distribution and impact of aerosol-delivered functionalized AuNPs upon repeated administration were explored in a complex in vitro human lung epithelial tissue barrier model applying an air–liquid interface exposure approach. Nanoparticles were aerosolized using the Air-Liquid Interface system consisting of a nebulizer, an exposure and an incubation chamber connected to an air-flow system to provide optimum conditions for cell cultivation as well as a quartz crystal microbalance for online measurements of the NP dose deposited on the cells surface.

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