Advanced in vitro exposure systems.

27. May. 2019

Characterization of the Vitrocell® Cloud SEQ 24 System and its Application in Exposure to Aerosolized Bortezomib and Ibuprofen

S. Steiner1, Sandra Ferreira1, Moran Morelli1, Audrey Baldi1, Arkadiusz K. Kuczaj1, Stefan Frentzel1, Marco van der Toorn1, Manuel Peitsch1, Julia Hoeng1
1 PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

This poster shows the investigation how aerosols are delivered within the Cloud SEQ 24 and how cell cultures respond to exposures in the system. Ibuprofen and Bortezomib was exposed to three dimensional organotypic tissue cultures of the human bronchial epithelium and biological endpoints were measured: Cytotoxicity, Interleukin 8 and Proteasome activity.

 

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5. Apr. 2019

Assessing the respiratory toxicity of dihydroxyacetone using an in vitro human airway epithelial tissue model

https://doi.org/10.1016/j.tiv.2019.04.007
Yiying Wanga, Qiangen Wub, Levan Muskhelishvilic, Kelly Davisc, Matthew Bryantb, Xuefei Caoa
a Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
b Division of Biochemistry Toxicology, National Center for Toxicological Research, Jefferson, AR 72079, United States of America
c Toxicologic Pathology Associates, Jefferson, AR 72079, United States of America

Unintentional exposure of respiratory mucosal surfaces to DHA aerosols is highly possible during sunless tanning sessions, a possibility for which it is not approved. In this study, the potential respiratory toxicity of DHA using the ALI airway tissue model is evaluated. To mimic in vivo exposure conditions, a liquid aerosol generation and exposure system was used and exposed ALI cultures to a range of non-cytotoxic doses of DHA aerosols at the air-liquid interface.


 

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6. Mar. 2019

Carbendazim induces death in alveolar epithelial cells: A comparison between submerged and at the air-liquid interface cell culture.

DOI: 10.1016/j.tiv.2019.03.004

Tollstadius BF, da Silva ACG, Oliveira BC, Pedralli, Valadares MC.

Laboratory of Education and Research in In vitro Toxicology, Faculty of Pharmacy, Universidade Federal de Goiás, Goiânia, GO, Brazil.

In this work, the toxicity of the fungicide Carbendazim upon A549 alveolar cells was tested in comparison of monolayer and air-liquid interface cell system exposition. 


 

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26. Jul. 2018

Hybrid Lipid/Polymer Nanoparticles for Pulmonary Delivery of siRNA: Development and Fate Upon In Vitro Deposition on the Human Epithelial Airway Barrier

DOI: 10.1089/jamp.2017.1364

Ivana d’Angelo, PhD,1 Gabriella Costabile, PhD,2,3 Estelle Durantie, PhD,3 Paola Brocca, PhD,4 Valeria Rondelli, PhD,4 Annapina Russo, PhD,5 Giulia Russo, PhD,5 Agnese Miro, PharmD,2 Fabiana Quaglia, PhD,2 Alke Petri-Fink, PhD,3 Barbara Rothen-Rutishauser, PhD,3 and Francesca Ungaro, PhD2
1 Di.S.T.A.Bi.F., University of Campania ‘‘Luigi Vanvitelli,’’ Caserta, Italy.
2 Laboratory of Drug Delivery, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.
3 Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland.
4 Applied Physics, Department of Medical Biotechnology and Translational Medicine, University of Milano, Milano, Italy.
5 Laboratory of Biochemistry, Department of Pharmacy, University of Napoli Federico II, Napoli, Italy.

The Vitrocell Cloud was used for the exposure to aerosolized nanoparticles on a in vitro three-dimensional cell cluture, mimicking the human epithelial airway barrier comprising human bronchial epithelial cells,human blood monocyte-derived macrophages and dendritic cells to show the optimized siRNA transport and delivery at lung.

 

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11. Mar. 2018

A setup to test toxicity of alcohol inhalation from consumer products

User Group Meeting 2018

Dr. Monique Manche1, Dr. Fabrice Nesslany2, Tobias Krebs3
1Laboratoires Anios, 59260 Lille-Hellemmes, France
2Laboratoire de toxicologie génétique, Institut Pasteur de Lille, 59000 Lille, France
3VITROCELL Systems GmbH, 79183 Waldkirch, Germany

 

Genotoxicity tests (in vitro micronucleus test and comet assay) have been conducted on p53 competent pulmonary cells, with alcohol administration as vapours to obtain data under conditions more representative of actual exposure conditions via the pulmonary route.

 

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11. Mar. 2018

Photometer Calibration Method

User Group Meeting 2018

Johannes Sood

VITROCELL Systems GmbH, 79183 Waldkirch, Germany

The poster shows a reliable method for the photometer calibration.

 

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11. Mar. 2018

New Products at VITROCELL® User Group Meeting 2018

10 March 2018, Grand Hyatt San Antonio, Texas, USA

Following the VITROCELL® User Group Meeting 2017 in Baltimore, international scientists from Canada, Europe, Japan and USA reconvened in March 2018.

The informal event organized into short presentations and posters was followed by open discussions. It was an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange your experience in working with the equipment and to meet other fellow researchers. The meeting took place prior to the international Society of Toxicology 57th Annual Meeting and ToxExpo, March 11 – 15, 2018, in San Antonio, USA - one of the largest international conferences related to toxicology.

Focus of the event was to share VITROCELL® activities since the last meeting, to exchange latest research results, to give an update on VITROCELL’s participation in major research programs as well as the presentation of new products for 2018.

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31. Aug. 2011

VITROCELL® Bio Aerosol Generator for use in turnkey in vitro exposure systems

New on WC8, Montreal and EUROTOX, Paris

An aerosol generator for smaller quantities of test aerosol operating at lower air flows.

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