Advanced in vitro exposure systems.

7. Mar. 2010

Aspects of nitrogen dioxide toxicity in environmental urban concentrations in human nasal epithelium.

Toxicol Appl Pharmacol.

2010 Jun 1;245(2):219-25. doi: 10.1016/j.taap.2010.03.003. [Epub ahead of print]

Authors
Koehler C., Ginzkey C., Friehs G., Hackenberg S., Froelich K., Scherzed A., Burghartz M., Kessler M., Kleinsasser N.

Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Germany

 

Nasal epithelial cells of were cultured in an air-liquid interface and exposed to NO(2). After exposure, genotoxicity was evaluated by the Comet assay and the micronucleus test. Depression of proliferation and cytotoxic effects were determined using the micronucleus assay and trypan blue exclusion assay.

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3. Mar. 2010

Evaluation of Dicarbonyls Generated in a Simulated Indoor Air Environment Using an in Vitro Exposure System

Toxicol Sci.

2010 Jun;115(2):453-61. doi: 10.1093/toxsci/kfq067. [Epub ahead of print]

Authors
Anderson SE, Jackson LG, Franko J., Wells JR.

National Institute for Occupational Safety and Health (NIOSH), 1095 Willowdale Drive, Morgantown, WV 26505

 

The air-liquid interface was used to determine whether compounds formed from indoor chemistry were capable of inducing inflammatory cytokine expression by exposed pulmonary epithelial cells (A549). Results are shown by measured IL-6 and IL-8, granulocyte-macrophage colony-stimulating factor and tumor necrosis factor alpha.

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14. Jan. 2010

A biological test for environmental toxins

BioMedical technology

Highlights from Baden-Württemberg, January 14, 2010

Authors
Dr. rer. nat. Richard Gminski

Institute of Environmental Medicine and Hospital Hygiene (IUK), Freiburg University Medical Centre / Germany

 

The researchers exposed human cells to the same environmental conditions to which we are exposed in our daily life.The objective is to ascertain how many cells survive and what happens inside the cells, and have discovered that this leads to comet-like effects on the DNA level. They also exposed human peripheral blood mononuclear cells and determined the type and quantity of cytokines when they were exposed to the gas. 

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16. Sep. 2009

Cytokine Production after Acute Exposure of Precision Cut Lung Slices to NO2 and O3

EUROTOX. Dresden, Germany.

September 13-16, 2009

Authors
K. Sewald, S. Switalla, D. Ritter, N. Krug, J. Knebel, A. Braun

Fraunhofer Institute for Toxicology and Environmental Medicine, Hannover, Germany

 

Precise cut lung slices were washed and exposed to synthetic air, O3 and NO2 in an air-liquid interface. Viability was controlled by Wst-1 and LIVE/DEAD® staining. Cytokines were quantified by ELISA or Luminex technology.

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16. Sep. 2009

An in vitro model to assess the impact on respiratory cells (A549) of air pollution

EUROTOX. Dresden, Germany.

September 13-16, 2009. Bo Holmstedt

Authors
Charles Persoz, Sophie Achard, Christopher Leleu, Isabelle Momas, Nathalie Seta

Laboratoire Sante Publique et Environment - EA 4064 - Universite Paris Descartes, France

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11. Sep. 2009

Biological effects in human lung cells exposed to platinum nanoparticle aerosol

European Aerosol Conference. Karlsruhe, Germany.

September 06-11, 2009

Authors
S. Diabaté, C. Weiss, S. Mülhopt, H.-R. Paur, V. Niedetzky, M. Seipenbusch

Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe, 76021, Karlsruhe, Germany
Institute for Technical Chemistry, Forschungszentrum Karlsruhe, 76021, Karlsruhe, Germany
Institute for Mechanical Process Engineering and Mechanics, University Karlsruhe, 76131, Karlsruhe, Germany

 

For the bioassay they exposed platinum nanoparticles to BEAS-2B and A549 cells, both co-cultured with differentiated THP-1 macrophages, growing on Transwell inserts. The responses of the cells were analyzed by measuring the viability, LDH and IL-8. 

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11. Sep. 2009

Toxic effects of nanoparticles from biomass combustion

European Aerosol Conference. Karlsruhe, Germany.

September 06-11, 2009

Authors
Werner Fr. Dreher, Frank Weise, Karen Böhme, Steffen Lutz, Michael Struschka, Johannes Brodbeck, Sonja Mülhopt, Hanns-Rudolf Paur, Silvia Diabaté

Materials Development and Testing, NMI Naturwissenschaftliches und Medizinisches Institut, Reutlingen, Germany
Institute of Process Engineering and Power Plant Technology (IVD), Universität Stuttgart, Stuttgart, Germany
Institute for Technical Chemistry, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
Institute for Toxicology and Genetics, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany

 

The toxicity of particulate matters, from the incomplete combustion of biomass in fire places, will be investigated. A549 human lung cell cultures are subjected to a constant flow of a conditioned aerosol. After exposure, the responses of the cells are analysed to measure the biological responses such as viability, inflammatory or oxidative stress. 

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11. Sep. 2009

The QCM - Online Dose Measurement with high relative Humidity

European Aerosol Conference. Karlsruhe, Germany.

September 06-11, 2009

Authors
S. Mülhopt, A. Comouth, A. Grotz, T. Krebs, H.-R. Paur

Institute for Technical Chemistry, Thermal Waste Treatment Division, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
Institute for Meteorology and Climate Research, Atmospheric Aerosol Research Department, Forschungszentrum Karlsruhe GmbH, Karlsruhe, Germany
VITROCELL Systems GmbH, Waldkirch, Germany

 

The shown method first time provides an online dose measurement for in vitro exposure experiments at the air-liquid interface also under high humidity conditions.  Human lung cell cultures were tested for different biological responses like the viability (LDH, AlamarBlue) as well as the release of IL-8.

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3. Sep. 2009

Air/Liquid Culture and Exposure Technique: Preliminary Results of a Prevalidation Study

World Congress on Alternatives and Animal Use in the Life Sciences. Rome, Italy.

August 30 - September 3, 2009

Authors
Smirnova, L.1, Liebsch, M.1, Tharmann, J.1, Pirow, R.1, Luch, A.1, Bauer, M.2, Graebsch, C.2, Linsel, G.3, Siemers, R.3, Berger-Preiß, E.4, Kock, H.4, Oertel, A.4, Riiter, D.4, Knebel, J.4

1 Federal Institute for Risk Assessment (BfR), Unit 37, Centre for Alternative Methods to Animal Experiments, ZEBET, Berlin, Germany
2 Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany
3 Federal Institute for Occupational Safety and Health (BAuA), Berlin, Germany
4 Fraunhofer Institute of Toxicology and Experimental Medicine (ITEM), Hannover, Germany

 

The aim of this study is to determine the in vitro vs. in vivo dose-response relationships. A549 cell were grwon in the air-liquid interface and exposed to NO2, SO2, formaldehyde and ozone. The results are measured in cytotoxicity (viability) and genotoxicity (COMET assay). The project has proved satisfying. transferability of the test method.

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31. Aug. 2009

SOT Meeting Baltimore

SOT Conference from March 15-19, 2009 in Baltimore/USA

VITROCELL® presented turnkey in vitro test systems for nano particles, gases and complex mixtures at the 2009 SOT Annual Meeting in Baltimore.

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