August 21-25, 2011
Singal M.1, Vitale D.1, Smith L.1, Czollner K.2, Farmer L.2, Höpfner C.2, Satzer H-P.2, Schoeffmann S.2, Schwartz B.2, Weber E.2
1 Research Institute for Fragrance Materials, Inc., Woodcliff Lake, NJ, USA
2 Seibersdorf Labor GmbH, Seibersdorf, Austria
This article shows a method for the identification of chemical respiratory allergens. Two respiratory allergens were applied to a 3D co-culture cell system model at the air/liquid interface, mimicking the in-vivo situation in the lung.
The results are shown in cytotoxicity (Propidium iodide, PI), Oxidative burst (Dihydrorhodamine assay, DHR), Pro- and antiinflammatory cytokines IL-2, IL-8, IL-10, IL-13, CD54, TNF-α, IFN-γ, MCP-1, RANTES (Cytometric Bead Array, CBA) and Staining of dendritic cell maturation marker CD86