https://doi.org/10.1007/s00204-019-02565-9

Anita R. Iskandar, Filippo Zanetti, Diego Marescotti, Bjorn Titz, Alain Sewer, Athanasios Kondylis, Patrice Leroy, Vincenzo Belcastro, Laura Ortega Torres, Stefano Acali, Shoaib Majeed, Sandro Steiner, Keyur Trivedi, Emmanuel Guedj, Celine Merg, Thomas Schneider, Stefan Frentzel, Florian Martin, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland

Previous experimental setups shows the effects of e-liquids on cell viability (first layer), followed by investigating the potential mechanisms of toxicity elicited by e-liquids (second layer) and finally assessing the impacts of aerosols (third layer). In this present work shows how the three-layer framework is leveraged to evaluate the potential toxicity and biological effects of the MESH Classic Tobacco and Base e-liquids/aerosols compared with those of 3R4F CS.

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Albert Giralt, Florian Martin, Anita R. Iskandar, Alain Sewer, Laura Ortega Torres, AthanasiosKondylis, Patrice Leroy, Celine Merg, ShoaibMajeed, Emmanuel Guedj, Thomas Schneider, KeyurTrivedi, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng

PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud5, CH-2000 Neuchâtel, Switzerland
 

In contrast to 3R4F CS exposure, exposure to IQOS MESH™ Classic Tobacco aerosols did not cause tissue damage or have an impact on ciliary beating functionality in bronchial epithelial cell cultures despite resulting in greater concentrations of deposited nicotine. Cultures exposed to IQOS MESH™ Classic Tobacco aerosols showed fewer changes in proteins involved in xenobiotic metabolism than those exposed to CS.

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DOI: 10.1021/acsnano.9b01823

Ingeborg Kooter¹, Marit Ilves ², Mariska Gröllers-Mulderij ¹, Evert Duistermaat ³, Peter C. Tromp ¹, Frieke Kuper ¹, Pia Kinaret ^4,5, Kai Savolainen ⁶, Dario Greco ^4,5, Piia Karisola ², Joseph Ndika ², and Harri Alenius <sup>2,7

1</sup>The Netherlands Organization for Applied Scientific Research, TNO, P.O. Box 80015, Utrecht 3584 CB, The Netherlands
²Human Microbiome Research, Faculty of Medicine, University of Helsinki, P.O. Box 21, Helsinki 00290, Finland
³Triskelion B.V., P.O. Box 844, Zeist 3704 HE, The Netherlands
⁴Faculty of Medicine and Life Sciences, University of Tampere, Tampere FI-33014, Finland
⁵Institute of Biotechnology, University of Helsinki, P.O. Box 56, Helsinki 00014, Finland
⁶Finnish Institute of Occupational Health, P.O. Box 40, Helsinki 00014, Finland
⁷Institute of Environmental Medicine, Karolinska Institutet, P.O. Box 210, Stockholm SE-17176, Sweden

3D human bronchial epithelial cells were cultured at the air−liquid interface that mimics relevant inhalatory exposure were exposed to aerosols of pristine (nCuO) and carboxylated (nCuOCOOH) copper oxide nanoparticles. This paper shows that the existence of asthma enhances sensitivity of the airways to nanoparticle aerosols, possibly as a combined result of a hyperactive airway and inefficient mucociliary clearance mechanisms in asthmatics. The test results are shown in cell viabilty (LDH), Inflammation (IL6, IL8, MCP1) and Transcroptomics.

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The congress will offer a change to tackle with the burning chemical safety challenges of the next decade. The theme of the Congress is “Toxicology – Science Providing Solutions”.

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Weber P. ¹, Chary A.^1,2, Saibene M. ¹, Serchi T.1, Cambier S.¹ , Moschini E.¹, Contal S.¹, Hennen J.², Ezendam J.³, Blömeke B.², Gutleb A.C.^1
1 Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), Luxembourg. 
² Department of Environmental Toxicology, Trier University, Germany.
³Centre for Environmental Protection, National Institute for Public Health and the Environment (RIVM), The Netherlands.

The Aim of this study is the adaptation of a 3D-co-culture system previously developed by Klein et al.2013. Inclusion of dendritic cells to study respiratory sensitization processes and identification and evaluation of markers to assess the respiratory sensitizing potential of inhaled compounds.

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