Advanced in vitro exposure systems.

11. Mar. 2018

The use of human 3D reconstructed bronchial tissue to study the effects of cigarette smoke and e-cigarette aerosol on a wide range of cellular endpoints

SOT 57th Annual Meeting, 11 –15th March 2018

Lukasz Czekala1, Matthew Stevenson1, Liam Simms1, Nicole Tschierske1, Anna G. Maione2, Tanvir Walele3
1 Imperial Tobacco Ltd, 121 Winterstoke Road, Bristol, BS3 2LL UK
2 MatTek, 200 Homer Ave., Ashland, MA, USA
3 Fontem Ventures B.V., an Imperial Brands PLC Company, Radarweg60, 1043 NT Amsterdam

A 3D in vitro organotypic model of the human respiratory epithelium was cultured at the Air-Liquid Interface (ALI) and exposed to cigarette smoke and e-cigarette aerosol, generated by using a VITROCELL® VC1 smoking machine under Health Canada Intense and CORESTA conditions.
The results are shown in tissue viability (MTT), barrier integrity (TEER), Barrier function, tissue secretion of the pro-inflammatory cytokines (IL-6 and IL-8). The presence of 8-isoprostane is used as an indicator of oxidative stress and antioxidant deficiency. Additionally the tissue morphology was assessed by H&E staining and immunofluorescent staining was conducted for specific markers.

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1. Mar. 2018

Comparative biological impacts of an aerosol from carbon-heated tobacco and smoke from cigarettes on human respiratory epithelial cultures: A systems toxicology assessment
Anita R. Iskandar, Florian Martin, Patrice Leroy, Walter K. Schlage, Carole Mathis, Bjorn Titz, Athanasios Kondylis, Thomas Schneider, Grégory Vuillaume, Alain Sewer, Emmanuel Guedj, Keyur Trivedi, Ashraf Elamin, Stefan Frentzel, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng
PMI R&D, Philip Morris Products S.A., Part of Philip Morris International group of companies, Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland

• Aerosols of a heat-not-burn tobacco product elicited lower impacts compared with smoke of 3R4F reference cigarettes.
• Systems toxicology revealed changes at the cellular–molecular levels not otherwise apparent from morphological features.
• The reduced impact of heated tobacco aerosols on small airway was similar than that on nasal epithelial cultures.

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1. Feb. 2018

Comparative Effect of a Candidate Modified Risk Tobacco Product Aerosol and Cigarette Smoke on Color Stability of Enamel, Dentin and Dental Composite Resins

SRNT 2018

Filippo Zanetti1, Xiaoyi Zhao2,3, Shoaib Majeed1, Hans Malmstrom2, Manuel C. Peitsch1, Yanfang Ren2, Julia Hoeng1

1 PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland (part of Philip Morris International group of companies)
University of Rochester Eastman Institute for Oral Health
, Rochester, New York
Peking University School of Stomatology
, Peking, China

In this study, the effects of 3R4F cigarette smoke and THS2.2 aerosol on color stability and surface roughness of premolar human teeth were investigated.

The poster was presented at SRNT 2018,

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18. Jan. 2018

Extreme testing of undiluted e-cigarette aerosol in vitro using an Ames air-agar-interface technique

D. Thornea, M. Hollingsb, A. Seymourb, J. Adamsona, A. Dalrymplea, M. Ballantyneb, M. Gacaa

a British American Tobacco, R&D, Southampton, Hampshire, SO15 8TL, UK
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

This study investigated the in vitro mutagenicity of e-cigarettes and designed a study to deliver e-cigarette aerosol on an undiluted per puff basis under extreme testing conditions, using the Ames assay across a broader range of bacterial tester strains. The Vitrocell VC 10 smoking robot, previously used to generate and dilute cigarette smoke aerosols was modified to enable the testing of an undiluted e-cigarette aerosol.

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15. Jan. 2018

An approach to testing undiluted e-cigarette aerosol in vitro using 3D reconstituted human airway epithelium
E. Bishop, L. Haswell, J. Adamson, S. Costigan, D. Thorne, M. Gaca
British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK

A reconstituted 3D human airway epithelium was exposed to undiluted aerosols at the air-liquid interface, using a Vitrocell VC 10 under ISO and HCI conditions. A 3R4F reference cigarette and a commercially available e-cigarette product were tested on their effect on CBF and TEER.

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13. Dec. 2017

The Society of Toxicology's 57th Annual Meeting and ToxExpo

March 11-15, 2018. Henry B. Gonzalez Convention Center – San Antonio, Texas, USA

The 57th Annual Meeting of the Society of Toxicology and ToxExpo will attract approximately 6.500 attendees in the fields of Medicine, Health, Biology, Chemistry, Pharmacology, Toxicology, Pathology and Anatomy.

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Categories: Exhibitions

Tags: congress

29. Nov. 2017

Biodistribution of single and aggregated gold nanoparticles exposed to the human lung epithelial tissue barrier at the air-liquid interface

DOI: 10.1186/s12989-017-0231-3

Estelle Durantie1, Dimitri Vanhecke1, Laura Rodriguez-Lorenzo1, Flavien Delhaes1, Sandor Balog1, Dedy Septiadi1, Joel Bourquin1Alke Petri-Fink1,2 and Barbara Rothen-Rutishauser1

1BioNanomaterials Group, Adolphe Merkle Institute, Université de Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland. 

2Chemistry Department, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland.


In this study, the biodistribution of aerosolized single and aggregated nanoparticles was investigated using a 3D model of the human epithelial tissue barrier.  The cells were exposed at the air-liquid interface using the Vitrocell® Cloud exposure system. It consists of three main parts: a nebulizer, an aerosol chamber and a base module constituted of 12-well size inserts and connected to a controlled heating unit. Robust characterization was used to evaluate the exact delivered dose onto the cell surface and to determine the cellular uptake and translocation across the barrier.

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26. Oct. 2017

12th International Conference and 5th Asian Congress on Environmental Mutagens (ICEM-ACEM 2017)

Nov 12–16, 2017. Incheon (Airport city of Seoul), Korea. Booth #12

It is a great pleasure to invite you to the 12th International Conference and 5th Asian Congress on Environmental Mutagens (ICEM-ACEM 2017), hosted by the Korean Environmental Mutagen Society (KEMS), to be held November 12-16, 2017, in Korea.

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Categories: Exhibitions

Tags: congress

26. Sep. 2017

8th National Congress of Toxicology (COST-VIII)

Oct 15–18, 2017 in Jinan, Shandong Province, China.

The congress is organized by Chinese Society of Toxicology (CSOT), and co-hosted by Shandong Academy of Medical Sciences and Shandong Society of Toxicology.

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Categories: Exhibitions

Tags: congress

17. Sep. 2017

Integration of Targeted Proteomics into Systems Toxicology Approach: Candidate Modified-Risk Tobacco Product Assessment using Nasal Cultures

HUPO 2017, Dublin

T. Schneider, A. Elamin, A.R. Iskandar, F. Zanetti, C. Merg, A. Sewer, B. Titz, P. Leroy, E. Guedj, S. Majeed, L. Ortega-Torres, W. Schlage, C. Mathis, F. Martin, S. Frentzel, N. V. Ivanov, M.C. Peitsch, J. Hoeng

Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland (Part of Philip Morris International group of companies)

The objectives of this study included to demonstrate robustness and reproducibility of systems biology data obtained from an organotypic in vitro nasal culture model for cigarette smoke and aerosol exposure assessment. Cellular stress and pro-inflammatory responses were investigated on the primary nasal epithelial cells of a non-smoker.

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