VITROCELL® News

30. Nov. 2019

Dosimetric Analysis of Aerosol Generated by a Vitrocell® VC10® Smoking Robot – Investigations on Dose Dependency and Consistency of Application

Adam Seymour, Michael Hollings, Joanne Larner and Julie Clements;

Covance Laboratories Ltd, Harrogate, UK

QCMs were used to assess the deposition of cigarette aerosol from a Vitrocell® VC10® in Vitrocell® Ames, 6/4, 12/4 and 24/4 exposure modules. The power to resolve difference between 8 and 1 SLPM is statistically significant in all module types assessed. Uniformity within each module was confirmed (P >0.98) in all module types, at all airflows. Repeatability was assessed and found to be consistent (no significant difference) in all module types at airflows of 10, 8 and 4 SLPM, however, at 1 SLPM a significant difference was noted in 9 out of 12 experiments – likely an artifact of high deposition.
These results show that exposures utilizing the Vitrocell® VC10® and associated modules are robust; but indicate the necessity of dosimetry measures to aid in the quantification of delivered dose.

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Categories: Publications

Tags: tobacco smoke, VITROCELL 24, AMES, VC 10 smoking robots, VITROCELL 6, microbalance, VITROCELL 12

27. Nov. 2019

State-of-the-art methods and devices for the generation, exposure, and collection of aerosols from heat-notburn tobacco products

Stéphanie Boué¹, Didier Goedertier¹, Julia Hoeng¹ , Arkadiusz Kuczaj¹, Shoaib Majeed¹, Carole Mathis¹, Anne May² , Blaine Phillips³, Manuel C Peitsch¹, Falk Radtke¹, Walter K Schlage⁴, Wei Teck Tan³ and Patrick Vanscheeuwijck¹

¹ Philip Morris International (PMI) Research & Development, Philip Morris Products S.A., Neuchâtel, Switzerland
² Consultants in Science, Epalinges, Switzerland
³ Philip Morris International (PMI) Research & Development, Philip Morris International Research Laboratories Pte. Ltd, Science Park II, Singapore
⁴ Biology Consultant, Bergisch Gladbach, Germany

The VC 24/48 exposure system is being validated for the exposure process of three-dimensional, organotypic cell culture inserts with CS and with aerosols generated from HNB tobacco products and e-liquids.
They aerosol deposition of different CS concentrations as determined by three different approaches were assessed and compared : (1) a WST-1 colorimetric assay; (2) the determination of eight carbonyls trapped in PBS; and (3) QCM-determined particle mass deposition.

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Categories: Publications

Tags: e-cigarettes, VITROCELL 24, air-liquid Interface, gas analysis

17. Nov. 2019

Society of Toxicology's 59th Annual Meeting and ToxExpo

March 15–19, 2020. Anaheim, California, USA

The 2020 scientific program has been designed to maximize exposure to groundbreaking research and opportunities for networking and professional development.

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Categories: Exhibitions

Tags: congress

4. Nov. 2019

Investigation of multiple whole smoke dosimetry techniques using a VITROCELL®VC10® smoke exposure system

https://doi.org/10.1016/j.toxrep.2019.10.011

Brian M. Keyser^a, Robert Leverette^a, Michael Hollings^b, Adam Seymour^b, Lesley Reeve^b, Wanda Fields^a
^a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC, 27101, USA1
^b Covance Laboratories Ltd., North Yorkshire, UK

Highlights
• Investigation of dose-determining methods using the Vitrocell® VC10® system.
• Dose assessment using Quartz Crystal Microbalances (QCM) and aerosol photometers.
• Dose assessment using fluorescence of DMSO-captured smoke constituents.
• QCM, photometer AUC, and DMSO-captured matter were consistent and reproducible

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Categories: Publications

Tags: tobacco smoke, VC 10 smoking robots, VITROCELL 6, microbalance, VITROCELL 12, photometer, air-liquid Interface

1. Nov. 2019

Electronic Cigarette Vapor With Nicotine Causes Airway Mucociliary Dysfunction Preferentially via TRPA1 Receptors

DOI: 10.1164/rccm.201811-2087OC

Samuel Chung ^{1 2}, Nathalie Baumlin ^{1 2}, John S Dennis ^{1 2}, Robert Moore², Sebastian F Salathe², Phillip L Whitney ², Juan Sabater ³, William M Abraham³, Michael D Kim ^{1 2}, Matthias Salathe ^{1 2}
¹Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Kansas.
²Division of Pulmonary, Critical Care and Sleep Medicine, University of Miami School of Medicine, Miami, Florida; and.
³Department of Research, Mount Sinai Medical Center, Miami Beach, Florida.

The transient receptor potential ankyrin 1 (TRPA1) is a molecular target for vape effects due to its expression in airway epithelia and its reported gating by nicotine, reactive oxidants, and flavors, especially cinnamaldehyde. To test whether nicotine had effects independent of other e-cig vapor constituents, the Vitrocell® CLOUD exposure system was utilized to nebulize fixed nicotine doses onto the apical surface of ALI cultures. A549 cell cultures were exposed to nicotine containing e-cig vapor, produced by the VC-1 smoke exposure robot, in the air-liquid interface.

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Categories: Publications

Tags: inhalation exposure, e-cigarettes, microbalance, VITROCELL Cloud, VC 1 smoking machine, air-liquid Interface

31. Oct. 2019

Deposition efficiency and uniformity of monodisperse solid particle deposition in the Vitrocell® 24/48 Air–Liquid-Interface in vitro exposure system

Aerosol Science and Technology

DOI: 10.1080/02786826.2019.1676877

Michael J. Oldham^a , Nicolas Castro^a, Jingjie Zhang^a, Ali Rostami^a, Francesco Lucci^b, Yezdi Pithawalla^a,
Arkadiusz K. Kuczaj^b,c , I. Gene Gilman^d, Pasha Kosachevsky^e, Julia Hoeng^b, and K. Monica Lee^a
^aAltria Client Services, Richmond, Virginia, USA;
^bPhilip Morris International Research & Development, Philip Morris Products S.A. (part of Philip Morris International group of companies), Neuchatel, Switzerland;
^cMultiscale Modeling & Simulation, Department of Applied Mathematics, University of Twente, AE Enschede, The Netherlands;
^dEnthalpy Analytical, Durham, North Carolina, USA; eEnthalpy Analytical, Richmond, Virginia, USA

Monodispersed solid particles were used to investigate experimental measurement of deposition efficiency, variability of deposition efficiency within and between rows and uniformity of deposition across all cell culture inserts of the Vitrocell 24/48 ALI in vitro exposure system.

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Categories: Publications

Tags: VITROCELL 24, nanoparticles, air-liquid Interface

25. Oct. 2019

VITROCELL® High Throughput Exposure Module: Deposition and Cytotoxicity of Smoke/Aerosol from Different Tobacco Product Types

User Group Meeting 2019

¹Leverette R, ¹Keyser B, ²Seymour A, ²Hollings M and ¹Bombick B
¹Scientific & Regulatory Affairs, RAI Services Company Winston-Salem, NC 27102
²Covance Laboratories Ltd Otley Road, Harrogate, North Yorkshire HG3 1PY, UK