DOI: 10.1164/rccm.201811-2087OC

Samuel Chung ^{1 2}, Nathalie Baumlin ^{1 2}, John S Dennis ^{1 2}, Robert Moore ², Sebastian F Salathe ², Phillip L Whitney ², Juan Sabater ³, William M Abraham ³, Michael D Kim ^{1 2}, Matthias Salathe ^{1 2
1}Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Kansas.
²Division of Pulmonary, Critical Care and Sleep Medicine, University of Miami School of Medicine, Miami, Florida; and.
³Department of Research, Mount Sinai Medical Center, Miami Beach, Florida.
 

The transient receptor potential ankyrin 1 (TRPA1) is a molecular target for vape effects due to its expression in airway epithelia and its reported gating by nicotine, reactive oxidants, and flavors, especially cinnamaldehyde. To test whether nicotine had effects independent of other e-cig vapor constituents, the Vitrocell® CLOUD exposure system was utilized to nebulize fixed nicotine doses onto the apical surface of ALI cultures. A549 cell cultures were exposed to nicotine containing e-cig vapor, produced by the VC-1 smoke exposure robot, in the air-liquid interface.

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¹Leverette R, ¹Keyser B, ²Seymour A, ²Hollings M and ¹Bombick B
¹Scientific & Regulatory Affairs, RAI Services Company Winston-Salem, NC 27102
²Covance Laboratories Ltd Otley Road, Harrogate, North Yorkshire HG3 1PY, UK

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Oliver Wisser¹, Bastian Gutmann¹, Adam Seymour^2
1VITROCELL Systems GmbH 79183 Waldkirch, Germany
²Covance Laboratories Ltd Harrogate, UK

The poster shows the new media inserts.

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DOI: 10.1016/j.chemosphere.2019.125126

Autors

King BM¹, Janechek NJ¹, Bryngelson N¹, Adamcakova-Dodd A², Lersch T³, Bunker K³, Casuccio G³, Thorne PS², Stanier CO⁴, Fiegel J⁵.

¹ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.
² Department of Occupational and Environmental Health, The University of Iowa, 145 N. Riverside Dr., Iowa City, IA, 52242, USA.
³ RJ Lee Group, 350 Hochberg Road, Monroeville, PA, 15146, USA.
⁴ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: charles-stanier@uiowa.edu.
⁵ Department of Chemical and Biochemical Engineering, The University of Iowa, 4133 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA. Electronic address: jennifer-fiegel@uiowa.edu.

Highlights
• Oxidative flow reactor used to study effects of secondary aerosols on lung cells.
• Nanoparticulate aerosols generated from OH oxidation of D5, a cyclic siloxane.
• Acute exposures to 54–116 ng/cm² achieved using the air-liquid interface (ALI) system.
• Cytotoxic and proinflammatory effects marginal or absent at these doses.

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Gaithersburg, Md. – The PETA International Science Consortium Ltd., Imperial Brands PLC, Altria Client Services (ALCS), British American Tobacco (BAT) PLC, and Philip Morris International Inc have joined together to donate equipment that can help to replace the use of animals in respiratory testing with more human-relevant, non-animal test methods.

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