DOI: 10.1080/17435390.2018.1472310

Beyeler S^1,2, Chortarea S^3,4, Rothen-Rutishauser B³, Petri-Fink A³, Wick P⁴, Tschanz SA⁵, von Garnier C^1,2, Blank F^1,2.

Read more

https://doi.org/10.1016/j.yrtph.2018.05.004

Shinkichi Ishikawa∗, Kazushi Matsumura, Nobumasa Kitamura, Kanae Ishimori, Yuichiro Takanami, Shigeaki Ito

The experimental setup for the aerosol exposure consisted of a VC 10 smoking robot, a dilution system, and a Vitrocell 12/3 glass module the exposure of nicotine and TPM in the mainstream aerosols from 3R4F and novel tobacco vapor on MucilAir™ cells. The results are shown in cytotoxicity, IL-8, IL-1ß and gene expression.

Read more

https://doi.org/10.1016/j.toxrep.2018.04.003

Kathy Fowler^a, Wanda Fields^a, Victoria Hargreaves^b, Lesley Reeve^b, Betsy Bombick^a,
^a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA2
^b Covance Laboratories Ltd., North Yorkshire, UK

The article shows the successfull qualification of the Vitrocell VC10 smoking robot. The robot was used to  expose bacterial cells to cigarette whole smoke generated from 3R4F reference cigarettes and Eclipse cigarettes. A comparison was made between whole smoke from a tobacco heating and a combustible cigarette.

Read more

DOI: 10.1089/aivt.2018.0001
Holger Behrsing,¹ Mario Aragon,¹ Jason Adamson,² Devin Sheehan,¹ Marianna Gaca,² Rodger Curren,¹ and Erin Hill^1
1Respiratory Toxicology, Institute for In Vitro Sciences, Inc., Gaithersburg, Maryland.
²R&D, British American Tobacco, Southampton, United Kingdom.

The study shows an assessment of VC1 aerosol generation that was conducted by measuring puff-by-puff in vitro nicotine concentration of 3R4F cigarettes (ISO and HCI regimes), e-cigarette (CRM81 puffing profile), and THP (HCIm regime). The aerosols were applied to in vitro or ex vivo cellular and tissue systems that model the respiratory tract, such as reconstructed human airways (RHuA), which are grown at the air–liquid interface (ALI). Additionally a comparison between the Vitrocell VC1 (U.S. Laboratory) and the VC10 (UK Laboratory) Smoking Robot was done.

Read more

Shinkichi Ishikawa, Kazushi Matsumura, Nobumasa Kitamura, Kanae Ishimori, Yuichiro Takanami, Shigeaki Ito
Scientific Product Assessment Center, Japan Tobacco Inc., 6-2, Umegaoka, Aoba-Ku, Yokohama, Japan

This Poster shows the exposure of nicotine and TPM in the mainstream aerosols from 3R4F and novel tobacco vapor on MucilAir™ cells using the Vitrocell® exposure system. The results are shown in cytotoxicity, IL-8, IL-1ß and gene expression.

Read more