Chary A.^1,2, Serchi T.1, Cambier S.1, Moschini E.1, Contal S.¹, Hennen J.², Ezendam J.³, Blömeke B.², Gutleb A.C.^1
1 Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology (LIST), Luxembourg.
² Department of Environmental Toxicology, Trier University, Germany. 3Centre for Environmental Protection,National Institute for Public Health and the Environment (RIVM), The Netherlands.

Aim
- Adapt a 3D-coculture system previously developed by us (Klein et al. 2013) allowing the study of respiratory sensitization processes by including dendritic cells, which have a crucial role in sensitization
- Find markers for the assessment of the respiratory sensitizing potential of inhaled compounds by measuring the activation of DCs as well as the release of cytokines

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Lukasz Czekala¹, Matthew Stevenson¹, Liam Simms¹, Nicole Tschierske¹, Anna G. Maione², Tanvir Walele^3
1 Imperial Tobacco Ltd, 121 Winterstoke Road, Bristol, BS3 2LL UK
² MatTek, 200 Homer Ave., Ashland, MA, USA
³ Fontem Ventures B.V., an Imperial Brands PLC Company, Radarweg60, 1043 NT Amsterdam

A 3D in vitro organotypic model of the human respiratory epithelium was cultured at the Air-Liquid Interface (ALI) and exposed to cigarette smoke and e-cigarette aerosol, generated by using a VITROCELL® VC1 smoking machine under Health Canada Intense and CORESTA conditions.
The results are shown in tissue viability (MTT), barrier integrity (TEER), Barrier function, tissue secretion of the pro-inflammatory cytokines (IL-6 and IL-8). The presence of 8-isoprostane is used as an indicator of oxidative stress and antioxidant deficiency. Additionally the tissue morphology was assessed by H&E staining and immunofluorescent staining was conducted for specific markers.

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Filippo Zanetti¹, Xiaoyi Zhao^2,3, Shoaib Majeed¹, Hans Malmstrom², Manuel C. Peitsch¹, Yanfang Ren², Julia Hoeng¹

¹ PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland (part of Philip Morris International group of companies)
² University of Rochester Eastman Institute for Oral Health, Rochester, New York
³ Peking University School of Stomatology, Peking, China

In this study, the effects of 3R4F cigarette smoke and THS2.2 aerosol on color stability and surface roughness of premolar human teeth were investigated.

The poster was presented at SRNT 2018, https://www.srnt.org/

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https://doi.org/10.1016/j.tiv.2018.01.010
E. Bishop, L. Haswell, J. Adamson, S. Costigan, D. Thorne, M. Gaca
British American Tobacco, R&D, Southampton, Hampshire SO15 8TL, UK

A reconstituted 3D human airway epithelium was exposed to undiluted aerosols at the air-liquid interface, using a Vitrocell VC 10 under ISO and HCI conditions. A 3R4F reference cigarette and a commercially available e-cigarette product were tested on their effect on CBF and TEER.

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DOI: 10.1186/s12989-017-0231-3

Estelle Durantie¹, Dimitri Vanhecke¹, Laura Rodriguez-Lorenzo¹, Flavien Delhaes¹, Sandor Balog¹, Dedy Septiadi¹, Joel Bourquin¹, Alke Petri-Fink^1,2 and Barbara Rothen-Rutishauser¹

¹BioNanomaterials Group, Adolphe Merkle Institute, Université de Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland. 

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