Advanced in vitro exposure systems.

27. Mar. 2022

Cytotoxicity Assessment of Electronic Nicotine Delivery Systems (ENDS) and Combustible Cigarette Aerosols Utilizing Standard and Whole Aerosol Exposure Approaches in the Neutral Red Uptake Assay

Poster SOT 2022, 61st Annual Meeting and ToxExpo, March 27-31, San Diego, USA

Robert Leverette1, Thomas Shutsky1, John Wertman1, Katarina Aleksa2, Dhatri Lakshmanan2, Rebecca Payne3, Kristen Jordan1
1Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC, USA; 
2Labstat International Inc. Kitchener, ON, Canada;
3Labcorp Early Development Laboratories Ltd., Harrogate, UK

This poster shows whole aerosol exposures of combustible and next generation tobacco products, including Electronic Nicotine Delivery Systems. A Vitrocell® VC10® robot generated and delivered aerosols to the Mammalian 6/48 aerosol dilution and exposure system, with up to 7 concurrent doses plus a clean air control. Dosimetry module allowed the capture and quantification of deposited aerosol constituents (nicotine, glycerol and carbonyls). The study utilized the Neutral Red Uptake (NRU) assay in which mammalian cells were exposed to either combined TPM + GVP (submerged culture) or Whole Aerosol (ALI).

 

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27. Mar. 2022

In Vitro Cytotoxicity Assessment of 3D Human Airway Tissue Following Exposure to Whole Aerosol/Smoke Generated from Electronic and Combustible Cigarettes

Poster SOT 2022, 61st Annual Meeting and ToxExpo, March 27-31, San Diego, USA

Thomas Shutsky1, Brian M. Keyser1, Kristen Jordan1, Michael Hollings2, and Emma Rothwell2
1Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC; 
2Labcorp Early Development Laboratories Ltd., Harrogate, UK

In this study, EpiAirway™ tissues were exposed to whole aerosol/smoke generated from six different Vuse Alto electronic nicotine delivery system test articles with various nicotine concentrations and a combustible cigarette. A Vitrocell® VC10® robot was used to generate whole aerosol/smoke with either a modified ISO or Health Canada Intense smoking regimen. Whole smoke/aerosol was puffed with clean air with a series of different air flows to achieve the delivered dose range. 

 

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1. Mar. 2022

The priming effect of diesel exhaust on native pollen exposure at the air-liquid interface

https://doi.org/10.1016/j.envres.2022.112968


Joana Candeiasa, Elias J. Zimmermannbc, Christoph Bisigb, Nadine Gawlittabc, Sebastian Oederb, Thomas Grögerb, Ralf Zimmermannbc, Carsten B. Schmidt-Webera, Jeroen Butersa
a Center Allergy & Environment (ZAUM), Member of the German Center for Lung Research (DZL), Technical University Munich / Helmholtz Center Munich, Germany
b Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics (CMA), Helmholtz Center Munich, Ingolstädter Landstraße 1, D-85764, Neuherberg, Germany
c Joint Mass Spectrometry Center (JMSC) at Analytical Chemistry, Institute of Chemistry, University of Rostock, Dr. Lorenzweg 2, D-18051, Rostock, Germany

 

Human bronchial epithelial BEAS-2B cells were exposed to native birch pollen (real life intact pollen, not pollen extracts) at the air-liquid interface (pollen-ALI). BEAS-2B cells were also pre-exposed in a diesel-ALI to diesel CAST for 2 h (a model for diesel exhaust) and then to pollen in the pollen-ALI 24 h later. Effects were analysed by genome wide transcriptome analysis after 2 h 25 min, 6 h 50 min and 24 h. Selected genes were confirmed by qRT-PCR.

 

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3. Feb. 2022

Product News 02-1/2022

VITROCELL® Cloud Advanced Nebulizer

For liquid droplet sizes 9 – 12 μm with diffusor

Certain chemicals or formulations, for example nasal sprays, often have larger particle sizes than the mesh size of standard nebulizers (4.0 – 6.0 μm). The VITROCELL Cloud Advanced Nebulizer is enlarging the application areas of Cloud Exposure Systems. This special type offers the possibility to produce an aerosol containing particles in the range of 9.0 – 12.0 μm.
Our patented Cloud diffusor ensures a homogeneous distribution of even the larger droplet sizes in the Cloud Exposure Chamber. To avoid effects from larger droplet formations it has an integrated drain channel.

VITROCELL® Cloud Advanced Nebulizer  (PDF)

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3. Feb. 2022

Effect of Atmospheric Aging on Soot Particle Toxicity in Lung Cell Models at the Air–Liquid Interface: Differential Toxicological Impacts of Biogenic and Anthropogenic Secondary Organic Aerosols (SOAs

https://doi.org/10.1289/EHP9413

Svenja Offer,1,2Elena Hartner,1,2Sebastiano Di Bucchianico,1Christoph Bisig,1Stefanie Bauer,1Jana Pantzke,1,2Elias J. Zimmermann,1,2Xin Cao,1,2Stefanie Binder,1,2Evelyn Kuhn,1Anja Huber,1Seongho Jeong,1,2Uwe Käfer,1,2Patrick Martens,2Arunas Mesceriakovas,3Jan Bendl,1,4,5Ramona Brejcha,1Angela Buchholz,6Daniella Gat,[...]

1 Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
2 JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany
3 Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland
4 Institute for Chemistry and Environmental Engineering, University of the Bundeswehr Munich, Neubiberg, Germany
5 Institute for Environmental Studies, Faculty of Science, Charles University, Prague, Czech Republic
6 Department of Applied Physics, University of Eastern Finland, Kuopio, Finland
7 Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, Rehovot, Israel
8 Institute of Energy and Climate Research, Troposphere, Forschungszentrum Jülich GmbH, Jülich, Germany
9 Department of Environmental Sciences, University of Basel, Basel, Switzerland
10 Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany
11 Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany

 

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2. Feb. 2022

LIVe 2022 – Lung in vitro event for innovative & predictive models

June 13 – June 14, 2022, Nice (France)

Lung in vitro event for innovative & predictive models

After a successful first edition in 2018, LIVe2022 is back in a bigger location: Hyatt Regency, Nice, France from Monday 13th to Tuesday 14th of June 2022. This edition will be a full two-days congress.

 

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Categories: Exhibitions

Tags: congress

2. Feb. 2022

MPS World Summit 2022

Inaugural Conference: May 30th – June 3rd, 2022, New Orleans, USA (Hybrid)

Connect, Exchange, Educate

Microphysiological System (MPS): cell culture systems replicating (patho)physiology through engineered organ architecture and functionality. This includes especially 3D-(co-)cultures such as organoids, organ-on-chip models, and multi-organ models, as well as the technologies to engineer and analyze these systems.

 

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Categories: Exhibitions

Tags: congress

2. Feb. 2022

2022 International Particulate Toxicology Conference IPTC

August 28–31, 2022, Santa Fe (USA)

 

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Categories: Exhibitions

Tags: congress

1. Feb. 2022

Automation and Standardization—A Coupled Approach Towards Reproducible Sample Preparation Protocols for Nanomaterial Analysis

https://doi.org/10.3390/molecules27030985

Jörg Radnik 1, Vasile-Dan Hodoroaba 1, Harald Jungnickel 2, Jutta Tentschert 2, Andreas Luch 2, Vanessa Sogne 3, Florian Meier 3, Loïc Burr 4, David Schmid 4, Christoph Schlager 5, Tae Hyun Yoon 6,7, Ruud Peters 8, Sophie M. Briffa 9 and Eugenia Valsami-Jones 9


1 Division 6.1, Federal Institute for Material Testing and Research (BAM), Unter den Eichen 44–46, 12203 Berlin, Germany;
2 Department of Chemical & Product Safety, German Federal Institute for Risk Assessment, Max-Dohrn-Strasse 8–10, 10589 Berlin, Germany; 
3 Postnova Analytics GmbH, Rankine-Strasse 1, 86899 Landsberg, Germany;
4 Centre Suisse d’Electronique et de Microtechnique (CSEM), Bahnhofstrasse 1, 7302 Landquart, Switzerland;
5 Vitrocell Systems GmbH, Fabrik Sonntag 3, 78193 Waldkirch, Germany; c.schlager@vitrocell.com
6 Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, Korea;
7 Institute of Next Generation Material Design, Hanyang University, Seoul 04673, Korea
8 Wageningen Food Safety Research, Wageningen University & Research, Akkermaalsbos 2, 6708 Wageningen, The Netherlands;
9 School of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UK;

 

The aim of this publication is to discuss how and when automated preparation can enhance the quality of the measurement results. For modern apparatuses, the measurement conditions are recorded and saved in the metadata automatically. As a result, the main reason for varying results is the different sample preparation. An actual interlaboratory comparison is ongoing to investigate the effect of different preparation methods systematically for ToF-SIMS. An air–liquid interface was developed to show that automation is possible for rather complex samples. Biological samples can be prepared in a reproducible manner, under exactly the same conditions on a TEM grid for the analysis of size and shape. Furthermore, chemical analysis can be performed by means of mass spectrometry.

 

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19. Jan. 2022

Product News 01/2022

VITROCELL® Photometer 2.0

Online measurement of particle concentrations

VITROCELL® Photometers are specifically designed and developed to enable online measurements of particle concen­trations. The working principe is light-scattering. They may be placed inline at various locations of the exposure system without particles losses – even at low flow rates.

VITROCELL® Photometer 2.0  (PDF)

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