Advanced in vitro exposure systems.

1. Jun. 2022

Product News 05-4/2022

VITROCELL® PowderX

For cell culture exposure to smallest quantities of dry powders

The VITROCELL® PowderX allows for aerosolisation of small quantities of dry powders and particle deposition on cells cultured on 12- or 24-well sized inserts. The system features biological barrier integration and continuous sampling from the basolateral compartment.

VITROCELL® PowderX  (PDF)

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11. Apr. 2022

Product News 04-3/2022

VITROCELL® Cloud Alpha AX12

Aerosol exposure for breathing AX Lung-on-Chip

The VITROCELL® Cloud Alpha AX12 is our newest innovation in the Cloud family and presents a great leap forward in automated exposure of cell cultures with breathing function. It combines highly efficient testing with ease of use. The development is based on the well-known and frequently published VITROCELL® Cloud formats (6-, 12-, 24- and 96-well).
Its functionality enables fully automated processes with an all-in-one control unit. Everyday experiments at the Air/Liquid Interface have never been easier.

It is suitable for the nebulization of solutions and suspensions. Fields of application are, but are not limited to, screening of inhaled drugs and toxicity testing of inhaled substances such as chemicals, nanoparticles and airborne pathogens.

VITROCELL® Cloud Alpha AX 12  (PDF)

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6. Apr. 2022

Product News 04-2/2022

VITROCELL® Smoking Machine VC 1 2.0

Manual smoking machine with high tech features

The VC 1 smoking machine is used for a controlled aerosol generation of combustion cigarettes as well as of next generation devices such as Heated Tobacco Products (HTP) and electronic nicotine delivery systems (ENDS). Special attention was given to guarantee very flexible programming of the smoking parameters. The properties of the aerosol are maintained due to small dead volumes of the system.

VITROCELL® Smoking Machine VC 1 2.0  (PDF)

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2. Feb. 2022

Smoking Machine VC 10 S-TYPE for Heat-not-Burn Tobacco Devices

Now Heated Tobacco Products (HTP) can be continuously smoked with automatic button actuation. The heat-and-turn feature for heat-not-burn devices enables automatic smoking at all 10 ports of the VC 10 S-TYPE Robot. Selective guidance of the aerosol to max. 6 exhaust lines is possible as well.

 

VITROCELL Application Note

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9. Dec. 2021

Iota-carrageenan extracted from red algae is a potent inhibitor of SARS-CoV-2 infection in reconstituted human airway epithelia

https://doi.org/10.1016/j.bbrep.2021.101187

David Bovard b, Marco van der Toorn b, Walter K. Schlage a, Samuel Constant c, Kasper Renggli b, Manuel C. Peitsch b, Julia Hoeng b,
a Biology Consultant, Max-Baermann-Str. 21, 51429, Bergisch Gladbach, Germany
b PMI R&D, Philip Morris Products S.A, Quai Jeanrenaud 5, 2000, Neuchaˆtel, Switzerland
c Epithelix Sarl, 18 Chemin des Aulx, Plan-les-Ouates, 1228, Geneva, Switzerland

 

This study successfully demonstrates the exposure of human bronchial epithelial cultures to defined doses of nebulized Iota-carrageenan which were reproducibly generated and administered using the VITROCELL Cloud 12 system. 
The aim of this study was to further investigate the efficacy and safety of IC treatment on SARS-CoV-2 infection by using advanced in vitro models of human respiratory epithelium, the primary target and entry port of SARS-CoV-2. The experimental models were 3D cultures of reconstituted bronchial and nasal epithelia, representing the surface of the human upper respiratory tract. This apical exposure of reconstructed epithelia more closely mimics real-life exposure conditions and the absence of toxicity or any functional or structural impairment of the bronchial mucociliary epithelium demonstrates that topical treatment with nebulized IC is well tolerated at the effective concentrations. 

 

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21. Jun. 2022

Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

https://doi.org/10.1016/j.envint.2022.107366

Michal Pardoa, Svenja Offerbc, Elena Hartnerbc, Sebastiano Di Bucchianicob, Christoph Bisigb, Stefanie Bauerb, Jana Pantzkebc, Elias J. Zimmermannbc, XinCaobc, Stephanie Binderbc, Evelyn Kuhnb, Anja Huberb, Seongho Jeongbc, Uwe Käferbc, Eric Schneiderc,[...]

a Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, 234 Herzl Street, POB 26, ISR-7610001 Rehovot, Israel
b Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics (CMA), Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
c Joint Mass Spectrometry Center (JMSC) at Analytical Chemistry, Institute of Chemistry, University of Rostock, Dr.-Lorenz-Weg 2, D-18059 Rostock, Germany
d Department of Environmental and Biological Sciences, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland
e University of the Bundeswehr Munich, Institute for Chemistry and Environmental Engineering, Werner- Heisenberg-Weg 39, D-85577 Neubiberg, Germany
f Institute for Environmental Studies, Faculty of Science, Charles University, Albertov 6, CZE-12800 Prague, Czech Republic
g Department of Applied Physics, University of Eastern Finland, Yliopistonranta 1, P.O. Box 1627, FI-70210 Kuopio, Finland
h Institute of Energy and Climate Research, Troposphere (IEK-8), Forschungszentrum Jülich GmbH, Wilhelm-Johen-Str., D-52428 Jülich, Germany
i Department of Environmental Sciences, University of Basel, Klingelbergstr. 27, CH-4056 Basel, Switzerland
j Max-Delbrück-Centrum für Molekulare Medizin (MDC), Robert-Rössle-Str. 10, D-13125 Berlin, Germany
k Institute of Computational Biology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, D-85764 Neuherberg, Germany
l The Mantoux Bioinformatics Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot 76100, Israel

 

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15. Jun. 2022

Characterization of Aerosol Deliveries from Combustible Cigarettes, Heated Tobacco Products, and Electronic Nicotine Delivery Systems Using the Vitrocell Ames 48

DOI: 10.1089/aivt.2022.0001

Brian M. Keyser,1 Robert Leverette,1 Michael Hollings,2 Adam Seymour,2 Randy J. Weidman,3 Carlton J. Bequette,3 and Kristen Jordan1
1 Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, North Carolina, USA.
2 Labcorp Early Development Laboratories Ltd., Harrogate, North Yorkshire, United Kingdom.
3 RJ Reynolds Tobacco Company, Winston-Salem, North Carolina, USA

 

This study utilized a Vitrocell Ames 48 exposure module climatic chamber. Cigarette smoke and eHTP/ENDS aerosol was generated using a Vitrocell VC10. Different concentrations of whole smoke or aerosol were achieved by altering the diluting airflow using mass flow controllers  The dosimetry measure used for assessing both combustible and NGTP aerosols in this characterization exercise was the laser photometer purchased from Vitrocell Systems and harmonized for each tobacco product type used in this study. To evaluate the multiple dosimetry techniques, pairwise comparisons were made between the two Vitrocell Ames 48 exposure modules for each of the three test articles. 

 

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Categories: Publications

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15. Apr. 2022

Aerosol–Cell Exposure System Applied to Semi-Adherent Cells for Aerosolization of Lung Surfactant and Nanoparticles Followed by High Quality RNA Extraction

https://doi.org/10.3390/nano12081362


Mélanie M. Leroux 1 , Romain Hocquel 1, Kevin Bourge 1 , Boštjan Kokot 2 , Hana Kokot 2 , Tilen Kokliˇc 2, Janez Štrancar 2, Yaobo Ding 3 , Pramod Kumar 3, Otmar Schmid 3 , Bertrand H. Rihn 1, Luc Ferrari 1 and Olivier Joubert 1
1 Institut Jean Lamour, UMR CNRS 7198, Université de Lorraine, CNRS, IJL, F-54000 Nancy, France
2 Jožef Stefan Institute, Department of Condensed Matter Physics, 1000 Ljubljana, Slovenia
3 Institute of Lung Health and Immunity, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany

The goal of this work was to refine an ALI pre-established protocol for the VITROCELL® Cloud 6 system. Therefore, after addressing each crucial step, (i.e., the use of very low concentrations of NP, the deposition of a homogeneous layer of surfactant, the specific use of semi-adherent cells, and finally the enhancement of the extraction yield of RNA to carry out transcriptomic studies), we present here for the first time a robust almost ready-to-use protocol. This one will be helpful for nanotoxicologists interested in developing such methodologies.

 

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4. Apr. 2022

Product News 04-1/2022

VITROCELL® Cloud Alpha PowerVent 

Increase of nebulizations and evacuation of residual gaseous compounds

A typical Cloud exposure allows 3 consecutive nebulizations. The PowerVent option enables an increase of the dose by adding further nebulizations. After each nebulization the humidity is removed by a short venting period before starting the new one. The venting function can be edited in the software. The PowerVent unit is delivered ready-to-use with an integrated vacuum pump.

VITROCELL® Cloud Alpha PowerVent  (PDF)

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27. Mar. 2022

Nrf2 Responses of Commercial Cigarette Whole Smoke and Aerosol from Six Electronic Nicotine Delivery Systems (ENDS) in a 3D Human Airway Model

Poster SOT 2022, 61st Annual Meeting and ToxExpo, March 27-31, San Diego, USA

Brian M. Keyser1, John Wertman1, Michael Hollings2, Robert Bedford2, and Kristen Jordan1
1 Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, NC; 
2 Labcorp Early Development Laboratories Ltd., Harrogate, UK

In this study whole smoke from a marketed combustible cigarette and whole aerosol from four different ENDS were evaluated on cell viability and Nrf2 response in a 3D human airway model transfected with a luciferase Nrf2 promoter.
Whole aerosol was generated using a Vitrocell ® VC10® Smoke Exposure System. The tissues were exposed to whole smoke from a market combustible generated under Health Canada Intense regime.These data show that the 3D Nrf2 EpiAirway™ in vitro model can be used to assess and discriminate responses from a biomarker (oxidative stress) for disease pathways associated with tobacco product usage.

 

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