Advanced in vitro exposure systems.

10. Mar. 2011

In vitro exposure of organotypical 3D epithelial tissues to cigarette smoke as a potential alternative to rodent inhalation studies

SOT Washington DC, USA

March 06-10, 2011

Authors
Weisensee, D., Kurkowsky, B., Hebestreit, M., Wagner, S. and Schueller J. 

Philip Morris Research Laboratories GmbH, Cologne, Germany, Abstract #998

 

Here we describe an in vitro test system that examines the toxicological effects of single or repeated cigarette smoke exposure on human organo- typically reconstituted 3D epithelial tissues. These tissues were exposed at the air liquid interface to either fresh air or to mainstream smoke from the Reference Cigarette 3R4F in the VITROCELL® system. 

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30. Jul. 2010

New on IUTOX

VITROCELL® Microbalance

The ideal tool for monitoring of partcle deposition in the exposure module.

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30. Jul. 2010

New on IUTOX

VITROCELL® Spiking Systems

The new tool to produce gas mixtures in the ppb range.

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23. Jul. 2010

Toxicological investigation of laser printer emissions - Effects on human cells

IUTOX. Barcelona, Spain.

July 16-23, 2010

Authors
Tao Tang, Richard Gminski, Rebecca Kuhn, Mathias Könczöl, Carsten Gründemann, Benedikt Armbruster, Volker Mersch-Sundermann

Institute of Environmental medicine and hospital epidemiology (IUK), University Hospital Freiburg, Breisacher Str. 115b, 79106 Freiburg, Germany

 

For this poster, human lung epithelial A549 cells and peripheral mononuclear blood cells were exposed to laser printer emissions on transwell inserts in a Vitrocell® airliquid interface exposure system. The results are shown with the WST-1-assay, cell proliferation, MNA, IL-2, IL-10 and IL-12.

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20. Jul. 2010

Untersuchungen zur genetischen Toxizität von Emissionen aus Laserdruckern in A549-Zellen im Vitrocell®-Transwell-Expositionssystem

Freiburg University Medical Centre

Authors
Tao Tang, Richard Gminski und Volker Mersch-Sundermann

Department of Environmental Health Sciences, Institute of Environmental Medicine and Hospital Hygiene (IUK), Freiburg University Medical Centre / Germany (Director: Univ.-Prof. Dr. med. habil. Volker H. Mersch-Sundermann)

 

In this german publication, human lung epithelial A549 cells and peripheral mononuclear blood cells were exposed to laser printer emissions on transwell inserts in a Vitrocell® airliquid interface exposure system. The results are shown with the WST-1-assay, cell proliferation, MNA, IL-2, IL-10 and IL-12.

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30. Jun. 2010

Effects of acute in vitro exposure of murine precision-cut lung slices to gaseous nitrogen dioxide and ozone in an air-liquid interface (ALI) culture

Toxicology Letters

2010 Jul 1;196(2):117-24. doi: 10.1016/j.toxlet.2010.04.004. [epub ahead of print]

Authors
S. Switalla, J. Knebel, D. Ritter, N. Krug, A. Braun, K. Sewald∗

Fraunhofer Institute for Toxicology and Experimental Medicine, Nikolai-Fuchs-Str. 1, 30625 Hannover, Germany

 

The publications shows a direct exposure to O(3) and NO(2) induced cytotoxicity and pro-inflammatory responses in precision-cut lung slices in an air-liquid interface culture. This provides a model that more closely resembles in vivo exposure of airborne contaminants.

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31. Mar. 2010

Cytotoxicity and genotoxicity in human lung epithelial A549 cells caused by airborne volatile organic compounds emitted from pine wood and oriented strand boards

Toxicology Letters

2010 Jun 16;196(1):33-41. doi: 10.1016/j.toxlet.2010.03.015. [epub ahead of print]

Authors
Gminski R, Tang T, Mersch-Sundermann V.

Institute of Environmental Medicine and Hospital Epidemiology, Department of Environmental Health Sciences, University Medical Center, Breisacher Strasse 115b, D-79106 Freiburg, Germany. richard.gminski@uniklinik-freiburg.de



The main constituents of wood-based materials, terpenes and aldehydes, shows an effect on A549 cells in cytotoxicity (erythrosine B staining) and genotoxicity (comet assay).

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31. Mar. 2010

SOT Meeting Salt Lake City, USA

VITROCELL® Quartz Crystal Microbalance System for nano particle dose measurement in turnkey in vitro exposure systems.

VITROCELL® presented advanced solutions for the exposure of cells to airborne nano particles, gases and complex mixtures at the 2010 SOT Annual Meeting in Salt Lake City / USA.

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25. Mar. 2010

A novel in vitro micronucleus (MN) test procedure after exposure of human derived lung cells to volatile chemicals at the air/liquid interface in vitro

Congress of the DGPT. Mainz, Germany.

March 23-25, 2010

Authors
Richard Gminski, Christoph Modest, Benedikt Armbruster, Tao Tang, Mathias Könczöl, Volker Mersch-Sundermann

Department of Environmental Health Sciences Institute of Environmental Medicine and Hospital Hygiene (IUK), Freiburg University Medical Centre / Germany

 

To develop a new bioassay for the air-liquid interface system, they optimized the conditions for the cytochalasin-block in vitro micronucleus test procedure using commercially available PTFE cell culture inserts. After exposure, cells were evaluated for cell viability (WST-1 assay) and for the induction of micronuclei.

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10. Mar. 2010

An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells

Toxicology Letters

2010 Jun 2;195(2-3):99-105. doi: 10.1016/j.toxlet.2010.03.003. [epub ahead of print]

Authors
Persoz C., Achard S., Leleu C., Momas I., Seta N.

Université Paris Descartes, Laboratoire de Santé Publique et Environnement - EA 4064, Paris, France

 

This study was thus set up to design an in vitro model, using a direct exposure device to study the cellular effects of air pollutants at environmental doses on lung epithelial cells and apply this to gaseous formaldehyde. Therefore A549 cells were exposed using the air-liquid interface to formaldehyde. After exposure, cellular viability (XTT) and inflammation (IL-6, IL-8 and MCP-1) were assessed.

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