Advanced in vitro exposure systems.

27. Mar. 2014

Cerium oxide nanoparticles air exposure: a comparison study using a human 3D airway model and A549 and Beas-2B cell lines

SOT Phoenix, USA

24-27 March, 2014

Authors
Ingeborg M. Kooter1, Mariska Grollers-Mulderij1, Maaike Steenhof1, Evert Duistermaat2, Frederique van Acker2, Yvonne Staal2, Eric Schoen1, Rob Stierum1, Linette Pellis1, Frieke Kuper1

1 The Netherlands Organisation for Applied Scientific Research, TNO, Zeist, The Netherlands
2 TNO Triskelion, Zeist, the Netherlands

Exposition of nano and macro particles on 3D lungcell models in comparison to normal lung cell lines in the air-liqid interface. Differentially effects are shown in the endpoints HO1, IL8, LDH, Comet Assay and gene expression.

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1. Nov. 2013

A review of in vitro cigarette smoke exposure systems

Experimental and Toxicologic Pathology

Volume 65, Issues 7-8, November 2013, Pages 1183-1193. pii: S0940-2993(13)00081-X. doi: 10.1016/j.etp.2013.06.001. [Epub ahead of print]

Authors
Thorne D, Adamson J.

British American Tobacco, Group R&D, Southampton SO15 8TL, UK.

In this review, the authors present a summary of the major tobacco smoke exposure systems available and critically review their function, set-up and application for in vitro exposure scenarios.

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3. Oct. 2013

A modified Ames methodology for the assessment of mainstream cigarette smoke genotoxicity using an aerosol-based exposure system

Poster CORESTA SSPT Meeting. Seville, Spain.

29 September - 3 October 2013

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Annette Dalrymple1, Julie Clements2, Clive Meredith1, Debbie Dillon1

1 British American Tobacco, Group R&D, Southampton, United Kingdom
2 Covance Laboratories Ltd, Harrogate, North Yorkshire, United Kingdom

 

The successfull exposure of whole smoke on a modified AMES assay using the the air-liquid interface exposure system. 

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30. Sep. 2013

Modelling and measurement of particle deposition for cell exposure at the air-liquid interface

Journal of Aerosol Science

Volume 63, September 2013, Pages 103-114

Authors
Andreas Comoutha, Harald Saathoffa, Karl-Heinz Naumanna, Sonja Muelhoptb, Hanns-Rudolf Paurb, Thomas Leisnera

a Institute for Meteorology and Climate Research, KIT, Karlsruhe, Germany
b Institute for Technical Chemistry, KIT, Karlsruhe, Germany

Presentation of a nanoparticle model to predict the size and particle deposition efficiencies for the air-liquid interface exposure system, that allows easy and accurate dose estimations. 

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30. Sep. 2013

In vitro reconstituted human airway epithelium model to assess the impact of indoor air pollutants on the inflammatory response

Poster EUROTOX 2013. Interlaken, Switzerland.

September 2013

Authors
Sophie Achard, Gaëlle Bardet, Sophie Grellet, Isabelle Momas, Nathalie Seta

Université Paris Descartes, PRES Cité Sorbonne, Faculté de Pharmacie; EA4064 Impact sanitaire des pollutions, 75006 Paris, France

 

The exposition of Formaldehyde as a major indoor air pollutant on a 3D nasal cell modell is demonstrated in the results if IL-8 and MCP assay.

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30. Sep. 2013

Comparison of epithelial cells inflammatory response from the human respiratory tract after formaldehyde exposure

Poster EUROTOX 2013. Interlaken, Switzerland.

September 2013

Authors
Gaëlle Bardet1,2, Charles Persoz1, Thomas Loret1, Isabelle Momas1, Sophie Achard1, Nathalie Seta1

1 Université Paris Descartes, Faculté de Pharmacie, EA4064 Impact sanitaire des pollutions, 75006 Paris, France
2 Agence de l’environnement et de la Maîtrise de l’Energie 20, avenue du Grésillé, BP 90406, 49004 Angers Cedex 01 France
Correspondence: gaelle.bardet@etu.parisdescartes.fr

 

The Exposition of Formaldehyde as a major indoor air pollutant on epithelial cells from different parts of the human respiratory tract is demonstrated in the results if IL-8 and MCP assay.

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30. Sep. 2013

A 4D lung multi-culture system mimicking alveolar cellular organization to study the toxic potential of airborne particles

Poster EUROTOX 2013. Interlaken, Switzerland.

September 2013

Authors
T. Serchi1,∗,S.G. Klein1,2, A. Jehanno1, S. Legay1, S. Contal1, J. Hennen2, A.C. Gutleb1, L. Hoffmann1 and B. Blömeke2

1 Dept. of Environment and Agro-biotechnologies (EVA), Centre de Recherche Public - Gabriel Lippmann, Luxembourg.
2 Department of Environmental Toxicology, University Trier, Germany.
*Corresponding author: serchi@lippmann.lu

 

A multicellular system of different cell types, that is exposed to airborne particles, shows its results in confocal laser scanning experiments.

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30. Sep. 2013

Evaluation of a human in vitro 3D respiratory epithelium model in bioavailability and safety assessment of pharmaceutical and chemical compounds

Poster EUROTOX 2013. Interlaken, Switzerland.

September 2013

Authors
Joost Vos1, Yvonne Staal1, Jos van Triel1, Frédérique van Acker1, Astrid Reus1, Evert Duistermaat1, Wilfred Maas1, Mariska Gröllers-Mulderij2, Maaike Steenhof2, Rob Stierum2, Linette Pellis2, Eugene van Someren2, Eric Schoen2, Frieke Kuper2 Ingeborg Kooter2

1 TNO Triskelion B.V., P.O.BOX 844, 3700 AV Zeist, The Netherlands
2 TNO, P.O.BOX 360, 3700 AJ Zeist, The Netherlands
For further information: joost.vos@tno.triskelion.nl

 

The poster shows that the respiratory epithelium model allows the assessment of bioavailability and safety of pharmaceutical and chemical compounds in the airways and lungs. The radio-labelled aerolosized nano particles are exposed to the cells on the air-liquid interface with the endpoints of TEER, HO-1, LDH, Comet-Assay and Illumina gene array expression analysis.

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6. Sep. 2013

Comet assay and air-liquid interface exposure system: A new combination to evaluate genotoxic effects of cigarette whole smoke in human lung cell lines

Toxicology in Vitro

Volume 27, Issue 6, September 2013, Pages 1987-1991. pii: S0887-2333(13)00168-9. doi: 10.1016/j.tiv.2013.06.016. [Epub ahead of print]

Authors
Weber S.1, Hebestreit M.1,3, Conroy L.L.1, Rodrigo G.2

1 Philip Morris International R&D, Philip Morris Research Laboratories GmbH, Fuggerstr.3, 51149 Cologne, Germany
2 Philip Morris International R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
3 Current address: VITROCELL Systems GmbH, Germany

 

Human lung cell lines A549 and BEAS-2B were exposed to diluted whole smoke in the VITROCELL® 24 and DNA damage was evaluated using the comet assay.

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3. Sep. 2013

Characterisation of a Vitrocell® VC 10 in vitro smoke exposure system using dose tools and biological analysis

Chemistry Central Journal 2013, 7:146

doi:10.1186/1752-153X-7-146

Authors
David Thorne1, Joanne Kilford2, Rebecca Payne2, Jason Adamson1, Ken Scott1, Annette Dalrymple1, Clive Meredith1 and Deborah Dillon1

1 British American Tobacco, Group R&D, Southampton, United Kingdom
2 Covance Laboratories Ltd, Harrogate, North Yorkshire, United Kingdom

 

The study demonstrated that the VC 10® can produce stable tobacco smoke dilutions by particulate deposition, measured vapour phase, smoke marker delivery and biological responses from two independent in vitro test systems. 

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