Advanced in vitro exposure systems.

3. Nov. 2016

Air-liquid interface exposure to aerosols of poorly soluble nanomaterials induces different biological activation levels compared to exposure to suspensions

DOI 10.1186/s12989-016-0171-3

Thomas Loret1,2, Emmanuel Peyret1, Marielle Dubreuil1, Olivier Aguerre-Chariol3, Christophe Bressot3, Olivier le Bihan3, Tanguy Amodeo3, Bénédicte Trouiller1, Anne Braun1, Christophe Egles2,4 and Ghislaine Lacroix1


1
Institut National de l’Environnement Industriel et des Risques (INERIS), (DRC/VIVA/TOXI), Parc Technologique ALATA—BP 2, Verneuil-en-Halatte F-60550, France.
2Laboratoire BioMécanique et BioIngénierie (BMBI), Université de Technologie de Compiègne (UTC), UMR CNRS 7338, Compiègne 60205, France.
3Institut National de l’Environnement Industriel et des Risques (INERIS), (DRC/CARA/NOVA), Parc Technologique ALATA—BP 2, Verneuil-en-Halatte F-60550, France.
4Department of Biomedical Engineering, Tufts University, Medford, MA, USA.
 

A549 alveolar cells in monocultures and in co-cultures with THP-1 macrophages were exposed to aerosols of inhalable and poorly soluble nanomaterials. The results are evaluated by using the Alamar blue®, LDH, Dichlorofluorescein (DCF) and ELISA Assay and shows that the air-liquid interface represents a valid and sensitive method to assess the toxicity of several poorly soluble nanomaterials.

 

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31. Oct. 2016

Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

doi: 10.1155/2016/9461289
Patrick Geraghty,1,2 Nathalie Baumlin,3 Matthias A. Salathe,3 Robert F. Foronjy,1,2 and Jeanine M. D’Armiento4
1Division of Pulmonary & Critical Care Medicine, Department of Medicine, State University of New York Downstate Medical Center, Brooklyn, NY, USA
2Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY, USA
3Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Miami, Miami, FL, USA
4Center for Pulmonary Disease, Department of Anesthesiology, College of Physicians and Surgeons,Columbia University, New York, NY, USA

 

The Expression of ER stress markers after exposure to cigarette smoke was investigated in fully differentiated normal human bronchial epithelial (NHBE) cells isolated from nonsmoking, smoking, and COPD donors and redifferentiated at the air liquid interface.

 

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28. Oct. 2016

Evaluation of an in vitro screening model to assess phosgene inhalation injury

DOI: 10.1080/15376516.2016.1243183

Dorian S. Olivera, Heidi Hoard-Fruchey & Alfred M. Sciuto
Analytical Toxicology Devision, US Army, Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, USA

In vitro studies have clearly shown that phosgene exposure can have overwhelming effects on cellular homeostasis with regard to mediator release, activation of inflammatory pathways and compromised lung function.
In this study an in vitro exposure model with which to evaluate potential therapeutics for edemagenic gas inhalation exposure was tested.
Human bronchial epithelial (16HBE) cell cultures were exposed in the Vitrocell System to phosgene.
Transepithelial electrical resistance (TEER), Cellular viability assay (XTT assay), [14C]-Glutamine oxidation assay, Glucose metabolism assays and Lactate production measurements were taken.

 

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27. Oct. 2016

Usefulness of toxicological validation of VOCs catalytic degradation by airliquid interface exposure system

doi: 10.1016/j.envres.2016.10.027.

Margueritta Al Zallouha, Yann Landkocz, Julien Brunet, Renaud Cousin, Eric Genty, Dominique Courcot, Stéphane Siffert, Pirouz Shirali, Sylvain Billet
Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, Université du Littoral Côte d′Opale, 189 A Avenue Maurice Schumann, 59140 Dunkerque, France


The exposure of A549 cells to VOCs and by-products at the Air-Liquid Interface exposure system were tested and showed toxic effects. Additionally a catalyst setup coupled to the Vitrocell system for testing by-products of toluene oxidation has been validated. The toxicological analysis is done with a cell viability test (LDH) and a TaqMan gene expression kit (RT-qPCR).

 

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9. Oct. 2016

The mutagenic assessment of electronic-cigarettes and tobacco smoke using the Ames assay in strains TA98 and TA100

CORESTA Congress 2016. 9-13 October 2016 - Berlin, Germany

CORESTA Congress, 9-13th October 2016, Germany
Poster No:STPOST34

 

Thorne, D1., Crooks, I1., Hollings, M2., Seymour, A2., Meredith, C1., Gaa, M1.

1 British American Tobacco, R&D Centre, Southampton, SO15 8TL, UK

2 Covance Laboratories Ltd, Otley Road, Harrogate, North Yorkshire HG3 1PY, UK.

 

The Aim of this study was to assess the mutagenicity of an e-cigarette aerosol, compared to cigarette smoke in tester strains TA98 and TA100 using two different exposure matrices, TPM/eTPM (or ACM – aerosol collected matter) and whole aerosol. A Vitrocell® VC 10 Smoking Robot was used to generate aerosol streams from a traditional reference cigarette (3R4F) and e-cigarette (Vype® ePen). 

 

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9. Oct. 2016

5 out of 7 Tobacco Companies have chosen VITROCELL® for research on combustion and e-cigarettes: A review of aerosol exposure systems relative to the analysis of cytotoxicity

CORESTA Congress 2016. 9-13 October 2016 - Berlin, Germany

CORESTA Congress, 9-13th October 2016, Germany

Poster No:STPOST26

 

David Thorne1, Roman Wieczorek2,Toshiro Fukushima3, Han-Jae Shin4, Robert Leverette5, Mark Ballantyne6, Xiang Li 7, Betsy Bombick5

1 British American Tobacco, Group R&D, Southampton, Hampshire, SO15 8TL, UK;
2 Imperial Brands PLC Company, Reemtsma Cigarettenfabriken GmbH, Albert-Einstein-Ring 7, 22761 Hamburg, Germany;
3 Japan Tobacco Inc, Scientific Product Assessment Centre, 6-2 Umegaoka, Aoba-ku Yokohama, Kanagawa 227-8512, Japan;
4 Korean Tobacco & Ginseng Corporation, 30 Gajeong-ro, Yuseong-gu, Daejeon, 305-805, Republic of Korea;
5 RAI Services Company, 401North Main Street, Winston-Salem, NC 27101, USA;
6 Covance Laboratories Ltd, Otley Road, Harrogate HG3 1PY, UK;
7 Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, No.2 Fengyang Street, High-tech Zone, Zhengzhou, PR China

 

Cytotoxicity data (NRU and MTT) from Kentucky reference 3R4F cigarette smoke were assessed on Balb/C 3T3, CHO-K1, A549, Beas-2B, NCI-H292 and HepG2 cell lines on the Vitrocell Exposure System.

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5. Oct. 2016

2nd International Conference on Nanomedicine 2016 (ChinaNanomedicine 2016)

18-21 Oct., 2016. Wuhan, China.

The ChinaNanomedicine conference in series will provide an insightful and multidisciplinary platform for discussions on novel functional nanomaterials with innovative capabilities for cutting-edge nanomedicine studies and applications, and their numerous challenges in clinical translation. Leading scientists and experts from academic community, clinical fields and industrial sectors worldwide will participate in the conference.

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Categories: Exhibitions

Tags: congress

9. Sep. 2016

VITROCELL® 产品中文彩页 / New VITROCELL® catalog in Chinese language

吸入式染毒设备与透皮仪器设备中文彩页,请至产品中心下载。
VITROCELL® is proud to present the first catalog with turnkey in vitro exposure systems for inhalation toxicology and skin exposure in Chinese language.

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9. Sep. 2016

21st Century Toxicology & In Vitro-Based Testing Methodologies

Julia Hoeng, Anita Iskandar, Filippo Zanetti

Philip Morris International


The studies used human cells grown in three-dimensional culture systems. The cultures were grown on top of an artificial membrane at the air-liquid interface, allowing them to develop ‘organotypic’ tissue complexity. Testcigarette is the Tobacco Heating System 2.2 (THS), a new technology that heats tobacco without burning it. The endpoints are shown in measurements of cytotoxicity, alterations in tissue morphology (histology), impact on processes involved in the metabolism of toxicants (xenobiotic metabolism response), secretion of pro-inflammatory mediators, and perturbation of genome-wide gene profiles. The nasal study also looked at ciliary function.



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8. Sep. 2016

11th International Particle Toxicology Conference

Sep 26 – 30, 2016 in Singapore

The International Particle Toxicology Conference is one of the longest standing scientific meeting series in its field.

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Categories: Exhibitions

Tags: congress

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