Advanced in vitro exposure systems.

16. Apr. 2017

4th Biological and Toxicological Conference

April 25–28, 2017. Hangzhou, China

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Categories: Exhibitions

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5. Apr. 2017

7th Conference of Alternative Methods

April 11–14, 2017. Guangzhou, China

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Categories: Exhibitions

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15. Mar. 2017

New Products 2017 presented at VITROCELL® User Group Meeting

10 March 2017, Hilton Baltimore Inner Harbor Hotel, USA

Following the VITROCELL® User Group Meeting 2016 in New Orleans, international scientists from Canada, Europe, Japan, Korea and USA reconvened in March 2017.

The meeting offered an excellent opportunity to discuss the latest developments of VITROCELL®, to exchange experiences in working with VITROCELL® devices and to meet fellow researchers. It was held prior to the International Society of Toxicology 56th Annual Meeting and ToxExpo – one of the largest international conferences related to toxicology.

Focus of the event was to exchange latest research results, to give an update on VITROCELL’s participation in major European and German research programs as well as the presentation of new products for 2017.

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6. Mar. 2017

Endothelial responses of the alveolar barrier in vitro in a dose-controlled exposure to diesel exhaust particulate matter

DOI 10.1186/s12989-017-0186-4

Sebastian G. Klein1,2, Sébastien Cambier1, Jennifer Hennen2, Sylvain Legay1, Tommaso Serchi1, Inge Nelissen3, Aline Chary1, Elisa Moschini1, Andreas Krein1, Brunhilde Blömeke2 and Arno C. Gutleb1

1Luxembourg Institute of Science and Technology (LIST), Environmental Research and Innovation (ERIN) Department, 41, rue du Brill, L-4422 Belvaux, Grand Duchy of Luxembourg
2Department of Environmental Toxicology, University Trier, Universitätsring 15, 54296 Trier, Germany


In this study tetracultures of alveolar epithelial cells (A549) were exposed to different doses of DEPM (SRM2975) at the air-liquid-interface to present the first approach to combine a complex 3D tetraculture in vitro system with native aerosol exposure equipment to mimic the exposure situation in vivo as closely as possible.

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1. Mar. 2017

Characterization and Application of the VITROCELL VC1 Smoke Exposure System and 3D EpiAirway Models for Toxicological and e-Cigarette Evaluations

DOI: 10.1089/aivt.2016.0035
Wanda Fields,1 Anna Maione,2 Brian Keyser,1 and Betsy Bombick1

1RAI Services Company, Scientific and Regulatory Affairs, Winston-Salem, North Carolina.
2MatTek Corporation, Ashland, Massachusetts.

The aim of this study was to assess the effects of aerosol from two reference combustible cigarettes and two e-cigarettes by using the VC1 smoking machine with the air-liquid interface and to evaluate donor-to-donor variability of three EpiAirway tissue donors with the endpoints viability, barrier integrity, and gene promoter/expression regulation.


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31. Jan. 2017

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

DOI: 10.1089/aivt.2016.0025

Jason Adamson1,* Xiang Li2,* Huapeng Cui2, David Thorne1, Fuwei Xie2, and Marianna Gaca1
1British American Tobacco, R&D, Southampton, United Kingdom.
2Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, PR China.
*Joint first authors.

A VC 10 Smoking Robot was designated to look at the generated aerosol nicotine concentration from different e-cigarettes compared with the 3R4F reference cigarette in two different laboratories. The aerosol was trapped and the nicotine concentration was analysed by different kinds of mass spectometry.
Nicotine assessment across the tested e-cigarette categories showed consistent delivery of nicotine per puff within products and that the method was sensitive enough to detect different levels of nicotine across products. Good overlap in nicotine results were obtained in the two different laboratories.

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3. Jan. 2017

Development, qualification, validation and application of the neutral red uptake assay in Chinese Hamster Ovary (CHO) cells using a VITROCELL® VC10® smoke exposure system

Wanda Fields a, Kathy Fowler a, Victoria Hargreaves b, Lesley Reeve b, Betsy Bombick a
a RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street,Winston-Salem, NC 27101, USA
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG1 3PY, UK

This study discribes the development, validation and application of the NRU assay in Chinese Hamster Ovary (CHO) cells, following exposure to fresh whole smoke generated with the VITROCELL®VC10®system. The VITROCELL® VC10® successfully provided consistent generation and delivery of whole smoke from a cigarette (3R4F) and a primarily “heat-not-burn” cigarette (Eclipse).

 

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30. Dec. 2016

The Society of Toxicology's 56th Annual Meeting and ToxExpo

March 13 - 15, 2017. Baltimore Convention Center - Baltimore, Maryland, USA

The Society of Toxicology 56th Annual Meeting and ToxExpo promises stimulating lectures and presentations on scientific breakthroughs, important education and professional training opportunities, and time to connect with old friends and create new collaborations.

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Categories: Exhibitions

Tags: congress

16. Dec. 2016

'Clean-burning' Fuels May be Worse for Your Lungs

Nala Rogers for 'Inside Science'

A report about artificial lung reveals surprising dangers from pellet burners and diesel-fueled ships at the University of Rostock and Helmholtz Zentrum München in Germany.

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7. Dec. 2016

The comparative in vitro assessment of e-cigarette and cigarette smoke aerosols using the gH2AX assay and applied dose measurements

http://dx.doi.org/10.1016/j.toxlet.2016.12.006

David Thorne, Sophie Larard, Andrew Baxter, Clive Meredith, Marianna Gaҫa

British American Tobacco, R&D Centre, Southampton, Hampshire, SO15 8TL, UK

 

H I G H L I G H T S
Cigarette smoke generates a dose dependent increase in double strand breaks.
E-cigarette aerosols did not generate any increases in double strand breaks above control levels.
E-cigarette exposures were approximately 28-fold greater than that of cigarette smoke exposures.
In vitro dosimetry ensures accurate interpretation of the data, and confirms delivery of test agent.

In vitro dosimetry allows cross-product, study and system comparisons.

 

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