Advanced in vitro exposure systems.

High Throughput Air Liquid Interface Exposure Modules: Characterization of Smoke/Aerosol Dosimetry and in vitro Mutagenicity and Cytotoxicity of Two Tobacco Product Types

31. Dec. 2019

Robert Leverette1, Brian Keyser1, Michael Hollings2 and Adam Seymour2
1RAI Services Company, Winston−Salem, NC 27101 USA
2Covance Laboratories Ltd., Harrogate, North Yorkshire HG3 1PY, UK

 

• Freshly generated whole smoke / aerosol from three different tobacco product types (3R4F, THP and ENDS) was consistently delivered within the AMES 48 and 6/48 exposure modules.
• Biological endpoints: 3R4F whole smoke induced increased revertant counts (Ames) and cytotoxicity (NRU) in the AMES 48 and 6/48 modules, respectively.
• Revertant counts (Ames) and cytotoxicity (IC50) values were comparable to those from the standard exposure modules when run under similar exposure conditions.
• Overall, the AMES 48 and 6/48 modules are deemed “Fit for Use" for the in vitrO evaluation of different tobacco product types (combustible, THP, ENDS).
 

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