Advanced in vitro exposure systems.

30. Apr. 2002

Exposure of human lung cells to native diesel motor exhaust-development of an optimized in vitro test strategy

Toxicology in Vitro

Volume 16, Issue 2, pp. 185-192, April, 2002

J.W. Knebel, D. Ritter, M. Aufderheide


The experimental set-up allows the investigation of native diesel exhaust using short distances for the transportation of the gas to the target cells. Cell viability was not influenced by the exposure to clean air, whereas dose-dependent cytotoxicity was found contingent on the dosage of exhaust.

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31. Mar. 2002

Responses of cultured lung epithelial cells to airborne diesel emissions

Diesel Engine Emissions Reduction Workshop

Paper issued March, 2002

J. C. Seagrave, J. D. McDonald, and J. L. Mauderly

Lovelace Respiratory Research Institute

Investigation of diesel exhaust on human lung cells. Results were evaluated by measurement of ATP, WST-1, LDH release, IL-8, Glutathione and alkaline phosphatase.

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1. Aug. 2001

In vitro exposure of isolated cells to native gaseous compounds - Development and validation of an optimized system for human lung cells


Exp Toxic Pathol 2001; 53: 373-386 accepted August 2, 2001

Detlef Ritter, Jan W. Knebel, and Michaela Aufderheide


Exposures of human lung fibroblasts (Lk004 cells) and human lung epithelial cells (HFBE-21 cells) to synthetic air, ozone and nitrogen dioxide established that cells could be treated long enough to detect biological effects of environmentally relevant gas mixtures. The analysis of viability (viable cell number, tetrazoliumsalt cleavage) and intracellular end-points (oxidized/reduced glutathione, ATP/ADP) showed that both gases induced relevant cellular changes. In exploratory experiments without cells, the reproducibility and quality of the gas/membrane contact in the air-liquid interface could be demonstrated.

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Tags: inhalation exposure

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