Advanced in vitro exposure systems.

31. Dec. 2002

A Method for the In Vitro Exposure of Human Cells to Environmental and Complex Gaseous Mixtures: Application to Various Types of Atmosphere

ATLA

Issue 30, pp. 433-441, 2002

Authors
Michaela Aufderheide, Jan W. Knebel and Detlef Ritter

 

This study presents the testing of various generated atmospheres under the same cell-exposure conditions and demonstartion of studying dose-dependent cytotoxic effects in human lung epithelial cells exposed to air contaminated with single gases or complex mixtures, such as diesel exhaust fumes and side-stream cigarette smoke.

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30. Jun. 2002

In vitro genotoxicity assay of sidestream smoke using a human bronchial epithelial cell line

Food and Chemical Toxicology

Volume 40, Issue 6, pp. 845-850, June, 2002

Authors
L. Wolz, G. Krause, G. Scherer, M. Aufderheide, U. Mohr

 

In this study, genotoxic effects of sidestream cigarette smoke at different concentrations were assessed using the alkaline comet assay. HFBE 21 cells were exposed to clean air, nitrogen dioxide or sidestream smoke. 

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30. Apr. 2002

Exposure of human lung cells to native diesel motor exhaust-development of an optimized in vitro test strategy

Toxicology in Vitro

Volume 16, Issue 2, pp. 185-192, April, 2002

Authors
J.W. Knebel, D. Ritter, M. Aufderheide

 

The experimental set-up allows the investigation of native diesel exhaust using short distances for the transportation of the gas to the target cells. Cell viability was not influenced by the exposure to clean air, whereas dose-dependent cytotoxicity was found contingent on the dosage of exhaust.

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31. Mar. 2002

Responses of cultured lung epithelial cells to airborne diesel emissions

Diesel Engine Emissions Reduction Workshop

Paper issued March, 2002

Authors
J. C. Seagrave, J. D. McDonald, and J. L. Mauderly

Lovelace Respiratory Research Institute


Investigation of diesel exhaust on human lung cells. Results were evaluated by measurement of ATP, WST-1, LDH release, IL-8, Glutathione and alkaline phosphatase.

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1. Aug. 2001

In vitro exposure of isolated cells to native gaseous compounds - Development and validation of an optimized system for human lung cells

URBAN & FISCHER

Exp Toxic Pathol 2001; 53: 373-386 accepted August 2, 2001

Authors
Detlef Ritter, Jan W. Knebel, and Michaela Aufderheide

 

Exposures of human lung fibroblasts (Lk004 cells) and human lung epithelial cells (HFBE-21 cells) to synthetic air, ozone and nitrogen dioxide established that cells could be treated long enough to detect biological effects of environmentally relevant gas mixtures. The analysis of viability (viable cell number, tetrazoliumsalt cleavage) and intracellular end-points (oxidized/reduced glutathione, ATP/ADP) showed that both gases induced relevant cellular changes. In exploratory experiments without cells, the reproducibility and quality of the gas/membrane contact in the air-liquid interface could be demonstrated.

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Categories: Publications

Tags: inhalation exposure

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