Advanced in vitro exposure systems.

22. May. 2015

Testing particle delivery of e-cigarettes

VITROCELL® Application Note for VC 10® S-TYPE Smoking Machine and Particle Photometers

The new application note shows a successfull monitoring of the particle delivery of different e-cigarettes on the setup of a VITROCELL VC 10® S-TYPE Smoking Machine combined with the VITROCELL® Particle Photometer and Monitor Software.

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22. May. 2015

Testing of conventional and e-cigarettes: using EpiAirway and EpiAlveolar with the VITROCELL® in vitro Exposure System

VITROCELL® VC1 Smoking Machine and 12/6 CF Exposure Module at the Air/Liquid Interface.

New MatTek application note shows a state-of-the-art in vitro set up by VITROCELL® Systems for testing combustion cigarettes as well as electronic cigarettes with the endpoints toxicity, oxidative stress, TEER, beating cilia, gene expression and mucus secretion.

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21. Mar. 2015

Automatisiertes Expositionssystem für Zellkulturen an der Gas-Flüssigkeits-Grenzschicht

Feinstaub in der Lunge

(Automated exposure system for cell cultures at the air/liquid interface / article in German)

Sonja Mülhopt, Tobias Krebs, Dr. Silvia Diabaté, Christoph Schlager, Dr. Hanns-Rudolf Paur


Zur Analyse von gasgetragenen Nano- und Feinstpartikel werden Bioassays mit menschlichen Lungenzellkulturen durchgeführt. Mit dem automatisierten Vitrocell-Expositionssystem können Aerosole reproduzierbar auf die Zellen aufgebracht werden.

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5. Mar. 2015

The mutagenic assessment of mainstream cigarette smoke using the Ames assay: A multi-strain approach

Mutation Research/Genetic Toxicology and Environmental Mutagenesis


David Thornea, Joanne Kilfordb, Michael Hollingsb, Annette Dalrymplea, Mark Ballantyneb, Clive Mereditha, Deborah Dillona

a British American Tobacco, Group R&D, Southampton, Hampshire SO15 8TL, UK
b Covance Laboratories Ltd., Otley Road, Harrogate, North Yorkshire HG3 1PY, UK


Wholesmoke Exposure with the VITROCELL® smoking robot VC 10® and the quartz crystal microbalance technology for maintaining the standard Ames Assay

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20. Feb. 2015

Repeated exposure to carbon nanotube-based aerosols does not affect the functional properties of a 3D human epithelial airway model

Nanotoxicology, Early Online: 1–11 (doi: 10.3109/17435390.2014.993344)

Savvina Chortarea1, Martin J. D. Clift1, Dimitri Vanhecke1, Carola Endes1, Peter Wick2, Alke Petri-Fink1, and Barbara Rothen-Rutishauser1
1Department of BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Marly, Switzerland and
2Laboratory for Materials-Biology Interactions, EMPA, Swiss Federal Laboratories for Materials, Science and Technology, St. Gallen, Switzerland

Exposition of Carbone Nanotubes on 3D lung models in the air-liquid interface exposure system to measure oxydative stress and inflammatory response.

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12. Feb. 2015

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

New video protocol Exposure of 3D cell cultures using VITROCELL® 24/48 system

jove, 12 Februray 2015, Issue 96 (doi: 10.3791/52325)

Diana Kuehn1, Shoaib Majeed1, Emmanuel Guedj1, Remi Dulize1, Karine Baumer1, Anita Iskandar1, Stephanie Boue1, Florian Martin1, Radina Kostadinova1, Carole Mathis1, Nikolai V. Ivanov1, Stefan Frentzel1, Julia Hoeng1, Manuel C. Peitsch1

1 Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A

The aim of this video protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke at the air-liquid interface to measure ciliary beating and TEER.

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12. Nov. 2014

Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke

Chemistry Central Journal 2014, 8:62


Shoaib Majeed1, Stefan Frentzel1, Sandra Wagner2, Diana Kuehn1, Patrice Leroy1, Philippe A. Guy1, Arno Knorr1, Julia Hoeng1 and Manuel C. Peitsch1

1 Philip Morris Research and Development, Quai Jeanrenaud 5, 2000 Neuchâtel, Switzerland
2 Eurofins Umwelt West GmbH, Vorgebirgsstraße20, D-50389 Wesseling, Germany


The connection of a VITROCELL® 24/48 system to a 30-port carousel smoking machine is shown. The exposure is to verify the cigarette smoke aerosol distribution, the utility of VITROCELL® crystal quartz microbalances and the amount of particles deposited per surface area. The results are shown in cell viability and WST-1 oxidation.

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30. Oct. 2014

Comparative In Vitro Toxicity Profile of Electronic and Tobacco Cigarettes, Smokeless Tobacco and Nicotine Replacement Therapy Products: E-Liquids, Extracts and Collected Aerosols

International Journal of Environmental Research and Public Health 2014

11325-11347; doi:10.3390/ijerph111111325

Manoj Misra, Robert D. Leverette, Bethany T. Cooper, Melanee B. Bennett and Steven E. Brown

Lorillard Tobacco Company, A.W. Spears Research Center, 420 North English Street, Greensboro, North Carolina 27405, USA


The in vitro exposure of tobacco and e-cigarettes that were smoked on a VITROCELL VC 10® smoking robot. The Results are shown with the NRU, IL-8, Ames and Micronucleus assay.

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30. Oct. 2014

An inter-machine comparison of tobacco smoke particle deposition in vitro from six independent smoke exposure systems

Toxicology in Vitro

Volume 28, Issue 7, October 2014, Pages 1320-1328 (doi: 10.1016/j.tiv.2014.06.012)

J. Adamsona, D. Thornea, G. Erringtona, W. Fieldsb, X. Lic, , R. Payned, T. Krebse, A. Dalrymplea, K. Fowlerb, D. Dillona, F. Xiec, , C. Mereditha

a British American Tobacco, Group R&D, Southampton SO15 8TL, UK
b R.J. Reynolds Tobacco Co., P.O. Box 1487, Winston-Salem, NC 27102, USA
c Zhengzhou Tobacco Research Institute of China National Tobacco Corporation,
No.2 Fengyang Street, High-Tech Zone, Zhengzhou, PR China
d Covance Laboratories Ltd., Otley Road, Harrogate HG3 1PY, UK
e Vitrocell® Systems GmbH, Fabrik Sonntag 3, 79183 Waldkirch, Germany


Comparison of different smoke exposure systems to demonstrate VC 10® suitability for tobacco smoke exposure in vitro. The results are shown in the particle deposition across the modules.

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8. Oct. 2014

The Response of Human Nasal and Bronchial Organotypic Tissue Cultures to Repeated Whole Cigarette Smoke Exposure

International Journal of Toxicology (October 8, 2014)

Marja Talikka1, Radina Kostadinova1, Yang Xiang1, Carole Mathis1, Alain Sewer1, Shoaib Majeed1, Diana Kuehn1, Stefan Frentzel1, Celine Merg1, Marcel Geertz1, Florian Martin1, Nikolai V. Ivanov1, Manuel C. Peitsch1, Julia Hoeng1

1 Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland


Whole Smoke Exposure on nasal and bronchial cells using a VITROCELL®24 system in comparison to animal testings.

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